Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 89257138

GI number: 89257138

Start: 1819695

End: 1820105

Strand: Reverse

Name: leuB [H]

Synonym: FTL_1887

Alternate gene names: 89257138

Gene position: 1820105-1819695 (Counterclockwise)

Preceding gene: 89257139

Following gene: 89257136

Centisome position: 96.0

GC content: 33.58

Gene sequence:

>411_bases
TTGTGGAGAAATATTGTCAATGAAGTCGCTAAAGATTATCCAAGTGTAAAAGTTAATCATATGTATGTTGATAATTGCGC
TATGCAAATGGTGCTTAATCCTAGTCAATTTGACGTTATGGTTACTGGTAACCTTTTTGGTGATATTATCTCTGATTTAG
CATCTGTATTACCTAGATCTATTGGCTTAGTACCATCTATAAGTTTAAATAAAGATGGTTTTGGATTATATGAGCCTTCT
GGAGGTTCTGCTTATGATATCAAAGGTCAAAACAAAGCAAATCCAATCGCACAGATATTGTCTGCATCATTAATGCTTTC
TTATTCATTTGGCTTAGTAACAGAAGCTGAGGATATTGCAAATGCGATCAACCTAACTCTTGAAGATGGCTTTAGAACTC
AAGATATATAA

Upstream 100 bases:

>100_bases
TACGATTAGAAATATTGTCATACAAGCATTCGAGAGAGCTACCCAACGTTCAAATAGACTTACATCTGTAGATAAGGCTA
ATGTTCTTGACACATCAAGG

Downstream 100 bases:

>100_bases
ACTGAAGGAACTAAACTCGCTTCTACTAAAGAATTTACTAATGAAATTATAAAAAACCTATAGCTAAGATAATCTATAAA
TTGACTCTAGTCATTTAGTC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MWRNIVNEVAKDYPSVKVNHMYVDNCAMQMVLNPSQFDVMVTGNLFGDIISDLASVLPRSIGLVPSISLNKDGFGLYEPS
GGSAYDIKGQNKANPIAQILSASLMLSYSFGLVTEAEDIANAINLTLEDGFRTQDI

Sequences:

>Translated_136_residues
MWRNIVNEVAKDYPSVKVNHMYVDNCAMQMVLNPSQFDVMVTGNLFGDIISDLASVLPRSIGLVPSISLNKDGFGLYEPS
GGSAYDIKGQNKANPIAQILSASLMLSYSFGLVTEAEDIANAINLTLEDGFRTQDI
>Mature_136_residues
MWRNIVNEVAKDYPSVKVNHMYVDNCAMQMVLNPSQFDVMVTGNLFGDIISDLASVLPRSIGLVPSISLNKDGFGLYEPS
GGSAYDIKGQNKANPIAQILSASLMLSYSFGLVTEAEDIANAINLTLEDGFRTQDI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=125, Percent_Identity=42.4, Blast_Score=100, Evalue=7e-22,
Organism=Homo sapiens, GI28178821, Length=109, Percent_Identity=41.2844036697248, Blast_Score=83, Evalue=6e-17,
Organism=Homo sapiens, GI28178819, Length=109, Percent_Identity=41.2844036697248, Blast_Score=83, Evalue=6e-17,
Organism=Homo sapiens, GI28178816, Length=109, Percent_Identity=41.2844036697248, Blast_Score=83, Evalue=7e-17,
Organism=Homo sapiens, GI4758582, Length=138, Percent_Identity=31.8840579710145, Blast_Score=68, Evalue=2e-12,
Organism=Homo sapiens, GI28178838, Length=124, Percent_Identity=33.0645161290323, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI87081683, Length=136, Percent_Identity=56.6176470588235, Blast_Score=165, Evalue=9e-43,
Organism=Escherichia coli, GI1788101, Length=109, Percent_Identity=41.2844036697248, Blast_Score=93, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=130, Percent_Identity=41.5384615384615, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17550882, Length=130, Percent_Identity=38.4615384615385, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144293, Length=139, Percent_Identity=35.9712230215827, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17505779, Length=138, Percent_Identity=35.5072463768116, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319830, Length=143, Percent_Identity=46.8531468531469, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=141, Percent_Identity=41.1347517730496, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324709, Length=141, Percent_Identity=39.7163120567376, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322097, Length=141, Percent_Identity=39.0070921985816, Blast_Score=87, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24643268, Length=130, Percent_Identity=36.9230769230769, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24643270, Length=130, Percent_Identity=36.9230769230769, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281362242, Length=131, Percent_Identity=42.7480916030534, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648872, Length=131, Percent_Identity=42.7480916030534, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI161078633, Length=138, Percent_Identity=37.6811594202899, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650122, Length=138, Percent_Identity=37.6811594202899, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161078635, Length=138, Percent_Identity=37.6811594202899, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161078637, Length=138, Percent_Identity=37.6811594202899, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161078639, Length=138, Percent_Identity=37.6811594202899, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24661184, Length=138, Percent_Identity=35.5072463768116, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 14792; Mature: 14792

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRNIVNEVAKDYPSVKVNHMYVDNCAMQMVLNPSQFDVMVTGNLFGDIISDLASVLPRS
CCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHH
IGLVPSISLNKDGFGLYEPSGGSAYDIKGQNKANPIAQILSASLMLSYSFGLVTEAEDIA
CCCCCCCEECCCCCEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECHHHHH
NAINLTLEDGFRTQDI
HHHEEEECCCEECCCC
>Mature Secondary Structure
MWRNIVNEVAKDYPSVKVNHMYVDNCAMQMVLNPSQFDVMVTGNLFGDIISDLASVLPRS
CCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHH
IGLVPSISLNKDGFGLYEPSGGSAYDIKGQNKANPIAQILSASLMLSYSFGLVTEAEDIA
CCCCCCCEECCCCCEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECHHHHH
NAINLTLEDGFRTQDI
HHHEEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]