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The map label for this gene is rlmB [H]

Identifier: 89255916

GI number: 89255916

Start: 493358

End: 494014

Strand: Direct

Name: rlmB [H]

Synonym: FTL_0509

Alternate gene names: 89255916

Gene position: 493358-494014 (Clockwise)

Preceding gene: 89255915

Following gene: 89255917

Centisome position: 26.02

GC content: 33.64

Gene sequence:

>657_bases
ATGAGTAGTTTAATTTATGGTATTCATGCTGTTGATAGTTTGGTAGAAGCTAATCAAGCAAAAGAAATATTGGTATTTAA
GAAGTCAAATGCCAATCATAAGATCGAAGGTATTATCTCTAAAGCTGAATCAAAAGGTTTAAAAGTTACTTTTATTGATA
GTTTTAAACAGCTTCCAGGGCGAGTCAGAAAAGATGCAAATCATCAAAATATCTTTGCAGTAGAGATAAATGATTTTAAA
ACATATTCTGAGAATGATATTGAGTTTCTAGTTCCTCAGGATAAAAATGCTTTTATTTTAATATTAGATAATGTTCAAGA
TCCTCATAATTTTGGTGCGTGTATACGTAGTGCACATTCTGCAGGTATTGATTTTATAATTATCCCTAAAGATAATAGCG
CGCCAGTCAATGCAACGGTTAAAAAAGTTGCTTGTGGTGCTGCTGAGCATACAAAGATTGTAGTTGTTACTAATCTTGCT
CGAGCAATCGAAAAACTCAAACAATTAGGAGTATGGATTATTGGTTTAGCAGGTGAGGCAGATGATAGCTTATATTCGAT
GAATCTAGTAGATTCTGTAGCGATAGTTGCTGGTGCAGAAGGTAGTGGTATGCGTCAGCGTACAAAAGCTATAGTTAATC
CGAGAGTATTTTGTTAA

Upstream 100 bases:

>100_bases
TATTAACTAAGCACCAAGATAGAAATATCCAGCTTGTAATGATGAGTAATGGCTCGTTTGATGGATTAAGAGAAAAGCTA
GTTAAGCTTTTGGAGATTAA

Downstream 100 bases:

>100_bases
ACACATAAGAGATAGCATCACAAAATTTTTCAAAACTATTATTCACTTTTCTAAATATTTTTTTAAAGTTAGCCCAAACC
TTTTCAATAGGATTTAAATC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSSLIYGIHAVDSLVEANQAKEILVFKKSNANHKIEGIISKAESKGLKVTFIDSFKQLPGRVRKDANHQNIFAVEINDFK
TYSENDIEFLVPQDKNAFILILDNVQDPHNFGACIRSAHSAGIDFIIIPKDNSAPVNATVKKVACGAAEHTKIVVVTNLA
RAIEKLKQLGVWIIGLAGEADDSLYSMNLVDSVAIVAGAEGSGMRQRTKAIVNPRVFC

Sequences:

>Translated_218_residues
MSSLIYGIHAVDSLVEANQAKEILVFKKSNANHKIEGIISKAESKGLKVTFIDSFKQLPGRVRKDANHQNIFAVEINDFK
TYSENDIEFLVPQDKNAFILILDNVQDPHNFGACIRSAHSAGIDFIIIPKDNSAPVNATVKKVACGAAEHTKIVVVTNLA
RAIEKLKQLGVWIIGLAGEADDSLYSMNLVDSVAIVAGAEGSGMRQRTKAIVNPRVFC
>Mature_217_residues
SSLIYGIHAVDSLVEANQAKEILVFKKSNANHKIEGIISKAESKGLKVTFIDSFKQLPGRVRKDANHQNIFAVEINDFKT
YSENDIEFLVPQDKNAFILILDNVQDPHNFGACIRSAHSAGIDFIIIPKDNSAPVNATVKKVACGAAEHTKIVVVTNLAR
AIEKLKQLGVWIIGLAGEADDSLYSMNLVDSVAIVAGAEGSGMRQRTKAIVNPRVFC

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=155, Percent_Identity=29.6774193548387, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790623, Length=211, Percent_Identity=43.1279620853081, Blast_Score=176, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324775, Length=124, Percent_Identity=32.258064516129, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 23730; Mature: 23598

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLIYGIHAVDSLVEANQAKEILVFKKSNANHKIEGIISKAESKGLKVTFIDSFKQLPG
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHCCH
RVRKDANHQNIFAVEINDFKTYSENDIEFLVPQDKNAFILILDNVQDPHNFGACIRSAHS
HHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
AGIDFIIIPKDNSAPVNATVKKVACGAAEHTKIVVVTNLARAIEKLKQLGVWIIGLAGEA
CCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHCCEEEEEECCCC
DDSLYSMNLVDSVAIVAGAEGSGMRQRTKAIVNPRVFC
CCCEEEEHHHHEEEEEECCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSLIYGIHAVDSLVEANQAKEILVFKKSNANHKIEGIISKAESKGLKVTFIDSFKQLPG
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHCCH
RVRKDANHQNIFAVEINDFKTYSENDIEFLVPQDKNAFILILDNVQDPHNFGACIRSAHS
HHHCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHH
AGIDFIIIPKDNSAPVNATVKKVACGAAEHTKIVVVTNLARAIEKLKQLGVWIIGLAGEA
CCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHCCEEEEEECCCC
DDSLYSMNLVDSVAIVAGAEGSGMRQRTKAIVNPRVFC
CCCEEEEHHHHEEEEEECCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA