Click here to switch to the map view.

The map label for this gene is mpl [H]

Identifier: 89255915

GI number: 89255915

Start: 491991

End: 493361

Strand: Direct

Name: mpl [H]

Synonym: FTL_0508

Alternate gene names: 89255915

Gene position: 491991-493361 (Clockwise)

Preceding gene: 89255912

Following gene: 89255916

Centisome position: 25.95

GC content: 31.73

Gene sequence:

>1371_bases
ATGTCAAAACATATTCATATTTTAGGTATCTGTGGTACTTTTATGGGTTCTTTAGCAGTATTAGCTAAACAAAAGGGATA
TAAAGTAACAGGTTCGGATCTTAATGTCTATCCACCAATGAGTACTTATCTCGAGTCTCAAGGCATAGAGATATTACAAG
GATTTGATTGTGATCAACTAGATACAAATCCTGATGAAATTATTATTGGTAATATCATGAAAAGAGGTATGCCAATAATA
GAAAAAATCCTCGCAGAAAAGTTAAACTATTTCTCAGGTCCAGAGTGGCTATATCAAAATATTCTTAAATATAAAAAAGT
AATTGCAATAGCTGGTACACATGGTAAAACTACAACCACTACAATGACTATCAAAATACTAGAGCAGGCTGGCTTAAATC
CGAGTTTTTTGGTTGGTGGTGTTAGTAGTGATTTTGGTGTTTCATCACGTTACACAGATTCTGAGTATTTTGTTATTGAA
GCTGATGAATATGATACGGCTTTTTTTGATAAGCGTTCAAAATTGATTCATTATGATCCAAGTATTTTTGTGATTAACAA
TATTGAATTTGATCATGCTGATATTTTCAAAGACATTGATGCAATATTTTGGCAATTTCACCAATTACTTAGAAAGATGC
CATCAACAGCAAAGATTATCTACAACGCTAAGAATGATAATGTACAAAAAATAATATCAATGGGATGTTGGTCAGAGTTG
GTTAAAGTAAATTCTGATTTAGGTATCAGTATAACCAAGCACACACTAGATTACTCAAAGTTTGAATTATGTGATATTAA
TGGTAATAGTGTTGAAGTATCATGGGGCTTAATTGGTGAGCATAATGCTCTTAATGCTATGAGTGCCTATGCTGTAGCAA
AGCAACTAAATATATCTGATGAAATGGTAAAAGACGCTTTAGAAAGCTTTAGAGGAGTTAAAAGACGTTTAGAAGTATTA
TCTCATCAAGATAATGTTACTTTATATGATGATTTTGCTCATCATCCAACCTCTATTAAGTTAACTTTAGAAGCAGTGCG
TAACAAAGCTAAAGATGCTTATGTAGTAGCTCTTATTGACCCACGTTCAAATACAATGCGTCAAGGCGATAATAAAGATA
ATTTACCGATGTCAATTATCGAAGCTGATAGAGTATTGTTGTATAATCATAGTTTATTAAAGTGGGATGCTAAAGAAGTT
CTCAAAAATAGTAATAATGTTGATTTTATAGCTGATGTTGATGATTTTGTTGATTGTGTTGATAAATTATTAACTAAGCA
CCAAGATAGAAATATCCAGCTTGTAATGATGAGTAATGGCTCGTTTGATGGATTAAGAGAAAAGCTAGTTAAGCTTTTGG
AGATTAAATGA

Upstream 100 bases:

>100_bases
CAAGTAAATATAGGTTAAGATACAATATCTATTAATATATTTAAGCTCAAGAAACTGATATAATTTAGCCACTTTATTTA
ATAGTTTTAAAAAGTTTTTA

Downstream 100 bases:

>100_bases
GTAGTTTAATTTATGGTATTCATGCTGTTGATAGTTTGGTAGAAGCTAATCAAGCAAAAGAAATATTGGTATTTAAGAAG
TCAAATGCCAATCATAAGAT

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- me so-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQLDTNPDEIIIGNIMKRGMPII
EKILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTTTMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIE
ADEYDTAFFDKRSKLIHYDPSIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSEL
VKVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISDEMVKDALESFRGVKRRLEVL
SHQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALIDPRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEV
LKNSNNVDFIADVDDFVDCVDKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLEIK

Sequences:

>Translated_456_residues
MSKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQLDTNPDEIIIGNIMKRGMPII
EKILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTTTMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIE
ADEYDTAFFDKRSKLIHYDPSIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSEL
VKVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISDEMVKDALESFRGVKRRLEVL
SHQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALIDPRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEV
LKNSNNVDFIADVDDFVDCVDKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLEIK
>Mature_455_residues
SKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQLDTNPDEIIIGNIMKRGMPIIE
KILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTTTMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIEA
DEYDTAFFDKRSKLIHYDPSIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSELV
KVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISDEMVKDALESFRGVKRRLEVLS
HQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALIDPRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEVL
KNSNNVDFIADVDDFVDCVDKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLEIK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=455, Percent_Identity=47.2527472527472, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1786279, Length=415, Percent_Identity=29.3975903614458, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 51529; Mature: 51397

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQL
CCCCEEEEEEHHHHHHHHHHHHHCCCCEEECCCCEECCCHHHHHHHCCCCEEECCCCCCC
DTNPDEIIIGNIMKRGMPIIEKILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTT
CCCCCCEEEHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCEEE
TMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIEADEYDTAFFDKRSKLIHYDP
EEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEECC
SIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
VKVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISD
HHCCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCH
EMVKDALESFRGVKRRLEVLSHQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALID
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHCCCCCEEEEEEEC
PRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEVLKNSNNVDFIADVDDFVDCV
CCCCHHHCCCCCCCCCEEEECCCEEEEEECCHHCCCHHHHHCCCCCCEEEECHHHHHHHH
DKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLEIK
HHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKHIHILGICGTFMGSLAVLAKQKGYKVTGSDLNVYPPMSTYLESQGIEILQGFDCDQL
CCCEEEEEEHHHHHHHHHHHHHCCCCEEECCCCEECCCHHHHHHHCCCCEEECCCCCCC
DTNPDEIIIGNIMKRGMPIIEKILAEKLNYFSGPEWLYQNILKYKKVIAIAGTHGKTTTT
CCCCCCEEEHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCCEEE
TMTIKILEQAGLNPSFLVGGVSSDFGVSSRYTDSEYFVIEADEYDTAFFDKRSKLIHYDP
EEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCEEEECC
SIFVINNIEFDHADIFKDIDAIFWQFHQLLRKMPSTAKIIYNAKNDNVQKIISMGCWSEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
VKVNSDLGISITKHTLDYSKFELCDINGNSVEVSWGLIGEHNALNAMSAYAVAKQLNISD
HHCCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCH
EMVKDALESFRGVKRRLEVLSHQDNVTLYDDFAHHPTSIKLTLEAVRNKAKDAYVVALID
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHCCCCCEEEEEEEC
PRSNTMRQGDNKDNLPMSIIEADRVLLYNHSLLKWDAKEVLKNSNNVDFIADVDDFVDCV
CCCCHHHCCCCCCCCCEEEECCCEEEEEECCHHCCCHHHHHCCCCCCEEEECHHHHHHHH
DKLLTKHQDRNIQLVMMSNGSFDGLREKLVKLLEIK
HHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]