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The map label for this gene is purK [H]

Identifier: 89255810

GI number: 89255810

Start: 369170

End: 370267

Strand: Direct

Name: purK [H]

Synonym: FTL_0399

Alternate gene names: 89255810

Gene position: 369170-370267 (Clockwise)

Preceding gene: 89255809

Following gene: 89255813

Centisome position: 19.47

GC content: 36.43

Gene sequence:

>1098_bases
ATGAAAATAGGTATTATCGGAGCTGGACAACTTGCCAGGATGCTAAGCTTAGCAGGTACACCTTTAGGTTTAGAGTTTCA
TTGTCTTGGTAAAAATGGTGATTGTGCTGAAGAAGTTGTAAAAACTGTTACAGATATTGAGCTAACAAAGGTAAATGATG
TTGTAGCTTGGGCAAAGCAGTTTGATGTTATAACTTTTGAAAATGAAAATATCTCTCATGAGCTTATCAAAGCTATAAAT
CATGAGGTGAGTGTGTATCCATCAGCTAAAGCTATAGCTATTTCTCAAGATCGTTTACTTGAGAAATCTTTTATGCAAGA
TCATGGTATTGCTACTGCAAAATTTGTAAATATCGATAGTTTGGCAAAATTGCAAAGTGCTGTTGATGATCATGGTCTGC
CAGCTATACTAAAAACTCGTCGATTTGGCTATGATGGTAAGGGCCAGTTTGTGATTAGATCACAAGAGGATATTACTAAA
GCATGGGATGTGCTCAAGGATGCTCCGGATGGGCTTATTTATGAGGCTTTTGTTGATTTTGATTATGAAGTTTCACAAAT
ATGTACAGCTGATCTAAAGGGTAATATCGCCTTTTATCCATTGGCTAGAAATACTCATAAGCAGGGTATTATTGTTGAAT
CAGAAGCACCTTTTGAAAATGTTGTACTTGCTGAAAAAGCACAACAAATCGCTAAAATTCTAGTCAAGGAGTTTGCATAT
GTTGGTACTTTGGCAATTGAGTTTTTTGTCAAGGGTGATGAGCTAATAGTCAATGAAATAGCGCCACGTGTGCATAATAG
CGGTCATTGGAGTATTGATGGCGCCGTCACATCACAATTTGAAAATCATGTGCGTGCTATTGCCGGATTGATTTTAGGTG
ATACAACTAGTCGTAAGACAGTTATGCTAAATTGTATCGGTGGTATGCCGGCAACAAAAGATTTAGCAGCTCTAGATAGA
GTTAAAATTCATAGTTATAACAAGGAGCCTCGTAAGGGACGTAAAGTAGGTCATTTAAACCTTAATTTAAATGATGAAAC
TGATGAGTATCAGCTACTTCAAGCTAAAAAATTAATTGCTCTTTCTGAAGAAATTTAG

Upstream 100 bases:

>100_bases
GCAACATACAGATATAAATATTGCAAAAGCACTAGCTGAGTTTAGAGCTGAGCAAACACGATTTGTTTTGGAAAATCCTG
ATCCTAGGGAGCACTAAATA

Downstream 100 bases:

>100_bases
ATTACTCCAAAAGCATTCTTTATCCAATTAATATTACTTTCATTGATCGCAATTAAATCAAAACGACACTGATAATCTTG
GTATTGAGGATTGTGTTGTA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAIN
HEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITK
AWDVLKDAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKTVMLNCIGGMPATKDLAALDR
VKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQAKKLIALSEEI

Sequences:

>Translated_365_residues
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAIN
HEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITK
AWDVLKDAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKTVMLNCIGGMPATKDLAALDR
VKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQAKKLIALSEEI
>Mature_365_residues
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAIN
HEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITK
AWDVLKDAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKTVMLNCIGGMPATKDLAALDR
VKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQAKKLIALSEEI

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=359, Percent_Identity=33.983286908078, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788155, Length=303, Percent_Identity=27.0627062706271, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324702, Length=363, Percent_Identity=32.7823691460055, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40286; Mature: 40286

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQ
CEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEHHHHHHHHHHH
FDVITFENENISHELIKAINHEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDS
CEEEEECCCCCHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHCCCCEEEEECHHH
LAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKDAPDGLIYEAFVDF
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECC
DYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKT
HHEEEEEEEECCCEEEHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCE
VMLNCIGGMPATKDLAALDRVKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQAKKLIA
EEEEEECCCCCCHHHHHHHHHEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
LSEEI
HCCCC
>Mature Secondary Structure
MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAEEVVKTVTDIELTKVNDVVAWAKQ
CEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEHHHHHHHHHHH
FDVITFENENISHELIKAINHEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDS
CEEEEECCCCCHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHHHHCCCCEEEEECHHH
LAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKDAPDGLIYEAFVDF
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECC
DYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFENVVLAEKAQQIAKILVKEFAY
CCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKT
HHEEEEEEEECCCEEEHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCE
VMLNCIGGMPATKDLAALDRVKIHSYNKEPRKGRKVGHLNLNLNDETDEYQLLQAKKLIA
EEEEEECCCCCCHHHHHHHHHEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHH
LSEEI
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8816818; 10984043 [H]