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The map label for this gene is lpd [H]

Identifier: 89255742

GI number: 89255742

Start: 299866

End: 301278

Strand: Direct

Name: lpd [H]

Synonym: FTL_0311

Alternate gene names: 89255742

Gene position: 299866-301278 (Clockwise)

Preceding gene: 89255741

Following gene: 89257203

Centisome position: 15.82

GC content: 38.92

Gene sequence:

>1413_bases
ATGAGTGATATTAAAACACAAGTTGTAGTTTTAGGTAGTGGTCCTGGTGGATATAGTGCGGCTTTTAGAGCAGCTGACTT
AGGATTAGAAGTTGTTTTAGTAGAGAGATATGCCGAGATCGGTGGTGTGTGCCTAAATGTGGGATGTATCCCATCAAAAG
CTATGTTGCATATTGCCAAAGTTATCAATGAGGCTCGTCATCTAGAATCTCTTGGTATAATCGAGATGGGTGGCCTAAAA
ATCAATAGAGAAAACCTCTTAAAATATAAAGATGGGGTTATCGGTAAGCTTACTGGTGGTCTAAAGGGTATGGCTCAGAT
GAGAAAAGTAAAAATCGTACAAGGTTACGGTAAATTTACTTCTGATAAAGAACTTGCTGTTGAGGCTGCTGATGGTAAAG
TTACAAAAATTGCTTTTTATAACTGTATTATTGCCGCGGGTTCTAGTGTTATTAAGCTACCTTTTGTACCAGAGGACGAT
AGAATTATTGACTCTACAGGCGCTCTTGAGATGAAAGAGATTCCAGAAACTATGCTCGTAGTTGGTGGTGGAATTATCGG
TCTTGAGATGGCACAAGTATATTCTGAGTTAGGTACGAAGATCACGGTTGTTGAGTTTGCTGATCAGCTTATGAATGGTG
TCGATAAGGATCTAGTCAAAGCTTATCAAAAAGTAAATAGTCGTTATGATGTGCGCCTAAAAACAGCTGTAACAGCTATG
GAAGCTAAAGAAGATGGTATCTATGTAACTATGGAAGGCGATCATCCTGCAAAAGATGAGAGATTCGATAGAGTACTTAT
GGCTATTGGTCGTAAACCAAATGGTAAACTAATTGATGCTGAAAAAGCAGGTGTTAAAGTTGATGAGAGAGGCTTTATCC
CAGTAGATAAGCAATTACGTACAAATGTGCCTCACATTTTTGCTATTGGAGATATTGTTGGTCAACCTATGCTTGCTCAT
AAAGCTGTACCGGAAGGTAGAATAGCTGCTGAAGTAATATCAGGACTAAATCATAGTTTTGATCCTTTAGTGATTCCTTC
AGTTGCTTATACTGATCCAGAGGTTGCTTGGGTTGGTGAGACTGAGACTTCTGCAAAAGCTAAAGGTATCAAGTATGAAA
AAGGTGTATTCCCATGGGCAGCTAGTGGTAGATCGCTAAGTATCGATAGATCAGAGGGTATAACAAAAATTCTATTTGAT
GAAAATCATAAGATTATTGGAGCTTCTATCGTTGGTACTCATGCTGGCGAGCTTATTTCAGAAGCAGCTCTTGCAATAGA
AATGGGTTGTGATGCTGAAGATATAGCTCTTACAGTACATCCACATCCAACTCTATCTGAAAGCTTAATGATGGCTACAG
AAGTTTATGAAGGTACTGCTACAGATCTTCCGCCGCAAAAGAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
CATAGAGTGATAGATGGCGCATTAGCAGCTAAATTCTTAACTAGATATTGTCAGATTTTATCTGATTTACGTGAAATCAT
AATGTAAGCGGAGTTTTAAA

Downstream 100 bases:

>100_bases
GGGGCTGTCCTAGATAAGTTTACTTTAAAGTCTCTCTAACCCAGTGTCTAATTAGCTTTAGTTGCTCTTTAGAATCACTG
TGGTTAAATCTCCACTCACA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAKVINEARHLESLGIIEMGGLK
INRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFTSDKELAVEAADGKVTKIAFYNCIIAAGSSVIKLPFVPEDD
RIIDSTGALEMKEIPETMLVVGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLRTNVPHIFAIGDIVGQPMLAH
KAVPEGRIAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGETETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGITKILFD
ENHKIIGASIVGTHAGELISEAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK

Sequences:

>Translated_470_residues
MSDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAKVINEARHLESLGIIEMGGLK
INRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFTSDKELAVEAADGKVTKIAFYNCIIAAGSSVIKLPFVPEDD
RIIDSTGALEMKEIPETMLVVGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLRTNVPHIFAIGDIVGQPMLAH
KAVPEGRIAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGETETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGITKILFD
ENHKIIGASIVGTHAGELISEAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK
>Mature_469_residues
SDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAKVINEARHLESLGIIEMGGLKI
NRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFTSDKELAVEAADGKVTKIAFYNCIIAAGSSVIKLPFVPEDDR
IIDSTGALEMKEIPETMLVVGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAME
AKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLRTNVPHIFAIGDIVGQPMLAHK
AVPEGRIAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGETETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGITKILFDE
NHKIIGASIVGTHAGELISEAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=41.7391304347826, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=28.5714285714286, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI291045266, Length=444, Percent_Identity=26.3513513513513, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI22035672, Length=463, Percent_Identity=27.8617710583153, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=441, Percent_Identity=25.1700680272109, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI226437568, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI21389617, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI65787454, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=64.5569620253165, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=30.4824561403509, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=27.3730684326711, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=29.5194508009153, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=39.0086206896552, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=28.2105263157895, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=441, Percent_Identity=26.530612244898, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=441, Percent_Identity=26.530612244898, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=24.8291571753986, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=192, Percent_Identity=28.6458333333333, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=462, Percent_Identity=41.5584415584416, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=30.4166666666667, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=27.6923076923077, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=40.8791208791209, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=27.0788912579957, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=29.3361884368308, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=29.3361884368308, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=29.3361884368308, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50528; Mature: 50397

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAK
CCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEHHHHHHCCEEEEECCCCHHHHHHHHH
VINEARHLESLGIIEMGGLKINRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFT
HHHHHHHHHHCCEEEECCEEECHHHHHHHCCCCEEECCCCHHHHHHHHHEEEEECCCCCC
SDKELAVEAADGKVTKIAFYNCIIAAGSSVIKLPFVPEDDRIIDSTGALEMKEIPETMLV
CCCEEEEEECCCCEEEEEEEEEHHHCCCCEEEECCCCCCCCEECCCCCEEHHHCCCEEEE
VGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
ECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCCCCCCHHHH
TNVPHIFAIGDIVGQPMLAHKAVPEGRIAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGE
CCCCEEEEEHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCCCCEEECCEECCCCCEEEECC
TETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGITKILFDENHKIIGASIVGTHAGELIS
CCCCHHHCCCEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
EAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK
HHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDIKTQVVVLGSGPGGYSAAFRAADLGLEVVLVERYAEIGGVCLNVGCIPSKAMLHIAK
CCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEHHHHHHCCEEEEECCCCHHHHHHHHH
VINEARHLESLGIIEMGGLKINRENLLKYKDGVIGKLTGGLKGMAQMRKVKIVQGYGKFT
HHHHHHHHHHCCEEEECCEEECHHHHHHHCCCCEEECCCCHHHHHHHHHEEEEECCCCCC
SDKELAVEAADGKVTKIAFYNCIIAAGSSVIKLPFVPEDDRIIDSTGALEMKEIPETMLV
CCCEEEEEECCCCEEEEEEEEEHHHCCCCEEEECCCCCCCCEECCCCCEEHHHCCCEEEE
VGGGIIGLEMAQVYSELGTKITVVEFADQLMNGVDKDLVKAYQKVNSRYDVRLKTAVTAM
ECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
EAKEDGIYVTMEGDHPAKDERFDRVLMAIGRKPNGKLIDAEKAGVKVDERGFIPVDKQLR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCEECCCCCCCCCHHHH
TNVPHIFAIGDIVGQPMLAHKAVPEGRIAAEVISGLNHSFDPLVIPSVAYTDPEVAWVGE
CCCCEEEEEHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCCCCEEECCEECCCCCEEEECC
TETSAKAKGIKYEKGVFPWAASGRSLSIDRSEGITKILFDENHKIIGASIVGTHAGELIS
CCCCHHHCCCEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEEECCHHHHHHH
EAALAIEMGCDAEDIALTVHPHPTLSESLMMATEVYEGTATDLPPQKKKK
HHHHEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]