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The map label for this gene is aceF [H]

Identifier: 89255741

GI number: 89255741

Start: 298257

End: 299852

Strand: Direct

Name: aceF [H]

Synonym: FTL_0310

Alternate gene names: 89255741

Gene position: 298257-299852 (Clockwise)

Preceding gene: 89255740

Following gene: 89255742

Centisome position: 15.73

GC content: 38.22

Gene sequence:

>1596_bases
ATGTCTATAGAGATAGTTAAAGTCCCTGATATTGGGGATTATGATAACGTTGATGTGATCGAGGTTAATGTTGCTGTAGG
TGATGTTATTGCTGAAGAAGATTCACTAATTACGTTAGAAACAGATAAAGCAAGTATGGAGGTTCCATCTCCATTTGCAG
GTAAGATCACTAAATTAACTGTAAAAGTTGGTGATAAAGTTTCTCAGGGAACTGCAATAATGGAAGTTGAGGTTGAGAGT
GCTGCTGATCAAGCTGCTACTACACAATCACAACCTCAAACAACTAGTTCAGCTCCTGTAGCTGCTACGACGAACCAGAT
TGTTGATGTCGAGGTTCCAGATATTGGCGACTATGATAGTGTTGATGTGATTGAGGTGTCTGTAAAAGTAGGTGATGAGA
TAGCAGAAGAGGATTCGCTAATTACGCTTGAAACAGATAAGGCTAGTATGGAAGTACCATCGCCTGTAGCGGGTAAGGTT
GTTGAAGTTATTACTAAGGTTGGTGACAAGGTTTCGCAAGGTAGTTTAATTCTTAAGGTCAAAACACAAGGTTCAGCACC
GGTAGAGCAAACTAGTTCACAACCAGCGCCAGCTAAACAAGAGCAAGCTAAACAGCAAGCTGCTACACCTGCTGCTCCAA
CGCCAGCATCAAGTTCGGTAAATGAGTATGCTGTAGATAATTCTAATGCACATGCGTCTCCTGCAGTGAGAAAGCTAGCA
CGAATTCTAAATATTGATCTAAGTAAGGTTAAAGCTACAGGGCGTAAAGGTCGTGTAACAAAAGAAGATTGTTATAACTA
TATTAAGCATGCTGTCACACAAGTTCAAACTGGTAAGGTCGCTGCTAGTGGTAGTGGTTTAGATCTTTTAGATGATCCTG
TTGTTGATTTTGCTAAGTTTGGTGAGATTGAAACTCAACCATTATCAAGAATTAACAAGATTAGTGCTAAGAATTTACAT
CGTAACTGGGTGAAGATTCCTCATGTTACATTCTATGATGATGCGGATGTCACAGACTTAGAAGAGTTCAGAAATGCTAA
GAAAGCCTTTGCTGAGAAAAAAGGTATTAAGATTACACCTTTATCATTCTTGGTTAAAGCTGCTGCAGTTGCATTACAAG
AGTTCCCAAGATTTAATAGCTCATTATCAAATGATGGTGAGAACTTAATTATCAAGAAATATTATAATATTGGTTTTGCT
GCAGATACTCCAGCTGGTTTAATGGTTCCAGTTGTCAAAGATGCTGATAAAAAGGGTATCATTGAAATATCAAAAGATAT
TATGGAGTTAGCTGGCAAAGCTCGTGATGGTAAACTTGGTGCAAAGGATATGACAGGTGCTACATTTACTATCTCAAGCT
TAGGCGTGTTAGGTACTACGTCATTTACGCCTATTATAAATATGCCAGAGGTGGCTATTATGGGTGTATCTAAGACAGCA
GTGAAGCCTATTTGGAATGGTAAAGAGTTTATTCCTAGAACTATGCTACCATTATCATTATCTACAGATCATAGAGTGAT
AGATGGCGCATTAGCAGCTAAATTCTTAACTAGATATTGTCAGATTTTATCTGATTTACGTGAAATCATAATGTAA

Upstream 100 bases:

>100_bases
GCAAAGTTGAGGCTAGCGAAGTTAAAGCTGCGATTGAGAAGTACAATATAGATCCAGAGCGTATGGCACCTTTTTATAGC
TAATTGGTAAGGAGATATTA

Downstream 100 bases:

>100_bases
GCGGAGTTTTAAAATGAGTGATATTAAAACACAAGTTGTAGTTTTAGGTAGTGGTCCTGGTGGATATAGTGCGGCTTTTA
GAGCAGCTGACTTAGGATTA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLTVKVGDKVSQGTAIMEVEVES
AADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDSVDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKV
VEVITKVGDKVSQGSLILKVKTQGSAPVEQTSSQPAPAKQEQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLA
RILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKFGEIETQPLSRINKISAKNLH
RNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNSSLSNDGENLIIKKYYNIGFA
ADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTA
VKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM

Sequences:

>Translated_531_residues
MSIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLTVKVGDKVSQGTAIMEVEVES
AADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDSVDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKV
VEVITKVGDKVSQGSLILKVKTQGSAPVEQTSSQPAPAKQEQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLA
RILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKFGEIETQPLSRINKISAKNLH
RNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNSSLSNDGENLIIKKYYNIGFA
ADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTA
VKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM
>Mature_530_residues
SIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLTVKVGDKVSQGTAIMEVEVESA
ADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDSVDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKVV
EVITKVGDKVSQGSLILKVKTQGSAPVEQTSSQPAPAKQEQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLAR
ILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKFGEIETQPLSRINKISAKNLHR
NWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNSSLSNDGENLIIKKYYNIGFAA
DTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTAV
KPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=30.6451612903226, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI31711992, Length=412, Percent_Identity=31.7961165048544, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=33.920704845815, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI203098816, Length=437, Percent_Identity=27.6887871853547, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI203098753, Length=437, Percent_Identity=27.6887871853547, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI260898739, Length=146, Percent_Identity=35.6164383561644, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786305, Length=528, Percent_Identity=53.030303030303, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1786946, Length=428, Percent_Identity=31.0747663551402, Blast_Score=191, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=30.4449648711944, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17560088, Length=443, Percent_Identity=29.5711060948081, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=206, Percent_Identity=39.3203883495146, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=33.6363636363636, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=439, Percent_Identity=30.2961275626424, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324258, Length=430, Percent_Identity=26.5116279069767, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI18859875, Length=451, Percent_Identity=32.5942350332594, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=33.0472103004292, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=33.0472103004292, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=191, Percent_Identity=37.696335078534, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56799; Mature: 56668

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLT
CCEEEEECCCCCCCCCEEEEEEEEEECCEEECCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKVGDKVSQGTAIMEVEVESAADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDS
EEECCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCC
VDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKVVEVITKVGDKVSQGSLILKV
CEEEEEEEECCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
KTQGSAPVEQTSSQPAPAKQEQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLA
ECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHH
RILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKF
HHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHC
GEIETQPLSRINKISAKNLHRNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITP
CCCCCHHHHHHHHHHHHHHHCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECH
LSFLVKAAAVALQEFPRFNSSLSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGI
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCH
IEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTA
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEECCHHH
VKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM
CCCCCCCHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLT
CEEEEECCCCCCCCCEEEEEEEEEECCEEECCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKVGDKVSQGTAIMEVEVESAADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDS
EEECCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCC
VDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKVVEVITKVGDKVSQGSLILKV
CEEEEEEEECCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
KTQGSAPVEQTSSQPAPAKQEQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLA
ECCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHH
RILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKF
HHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHC
GEIETQPLSRINKISAKNLHRNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITP
CCCCCHHHHHHHHHHHHHHHCCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECH
LSFLVKAAAVALQEFPRFNSSLSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGI
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCH
IEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTA
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEECCHHH
VKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM
CCCCCCCHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]