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The map label for this gene is dacF [H]

Identifier: 89056340

GI number: 89056340

Start: 3944393

End: 3945799

Strand: Direct

Name: dacF [H]

Synonym: Jann_3849

Alternate gene names: 89056340

Gene position: 3944393-3945799 (Clockwise)

Preceding gene: 89056337

Following gene: 89056350

Centisome position: 91.35

GC content: 66.03

Gene sequence:

>1407_bases
ATGTTCGTACGGAATCTTTTCGTCGCCCTTCTGGGCGTTCAAATCCTGTTTGGGGCCGTGGCTCCTGCGCAGGTCAACGC
CGCCCCCTACGCCGCCATGGTAATGGATGCGCGCAACGGCGAAGTGCTGCATTCGCGCAACGCCGATACCCGGCTGCACC
CGGCATCCCTGACCAAGATGATGACCCTCTACATCGCGTTCGAAGCGATCCGCCTGGGCGAGATCACGTTGGACACCGAG
GTCACGGTATCGGCCAATGCCGCAGCCGAGCCGCCCTCGCGCCTCGGCCTGAGTGCGGGCACAACGATCCGCCTGCGCTA
CTTGCTGCGGGCTGCCGCCATCCGGTCCGGCAACGATGCGGCAACAGCCATCGGAGAGGCCATTTCAGGGTCAGAGGCCG
CGTTCGCACGCCGCATGACGCGGACCGCCCGCGCCCTTGGCATGACACGAACCACCTTCCGCAACGCGCATGGCTTGACC
GAAGATGGCCACCTGTCGACAGCCCGCGACATGACGATGCTGGGACGGCGGCTGGTCTATGATTATCCCCAATATTACAA
CCTGTTCTCGCGGGTGACAGCCGATGCGGGCATCGCCACCATCCGCAACACCAACCGTGTCGTGTTGACCACGTATCGCG
GTGCCGACGGCATCAAGACCGGCTACACCCGCGCCGCCGGGTTCAACCTCGTAGCCTCAGCCCAACGGGGCGAAGAGCGG
ATCATCGCGACGGTTTTTGGCGGACGAAGCTCTGCTTGGCGCAACCAGCGGGTGATGGAATTGCTGGATATGGGGTTTGA
ACGCGCGCCCAGTCACGTCGCGCTAAGGACACCTCCGCTACCCACCTATGCCGATGATGGCAGCCCCGTGGGTCGAACGG
CGACACCGCCCGGACAGCGCGCGCCGGAGCGCTCCATGCGCCCGATATCGCGGCCTGGCGGCGGAGGCGCAGGCGTGGAC
GCCGAAGTGCTCGCCGCCATCAGCGAGGCAGTCGGAGCGGCGCTTCAGCCCGGTGGCGACGTGGCCGCAGACGTGGAAGC
CGCCTTGAGTTTCGTGGCGAGGCCGACGGACAGGCCTGCGCCAAGACCTGAACCGCAAGTCGCGGCAGAACCCGAACCAG
CCCCGATCCCCCAGCCGGAGCCTGAGCTTGTGACCCGCGCCTCCTCCACCGGGTCGCGCCATTTCGGAGTGTCTCTTGGC
CTGCAACCGTCCCATCACGCCGCCGAGCGATTGCTGTTGCGCACAGCGCTGGCCGAACTCGGAACCTTCGACACCGCGCT
GCGCGAAGTGCGCAATGTCACCCGGGGGTTCGATGCACGCTTTGCGGGCATGACCGAAGATCAGGCGTCGCGTGCCTGCG
CACGGCTGCAAGCGCGCGATGTCGCCTGCGAAACGTTTGGGCCATAG

Upstream 100 bases:

>100_bases
TGGAAATTTCCTCACAACGGCATGGATTTTGGGGAACACTTGCCGTACGACTAAAGGCAATCGCCGCAACAGACGGCACT
AGAGGCAGATAGAGCAGATC

Downstream 100 bases:

>100_bases
AGCGGCAGACAGGCTTGGATCCAAACAAGGGCGGCCCATCCGGAGCCGCCGTTTTCTTGTCATCTCCCTACGGGACGGTG
GGAGGGGTGAGGTCACCGTT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFVRNLFVALLGVQILFGAVAPAQVNAAPYAAMVMDARNGEVLHSRNADTRLHPASLTKMMTLYIAFEAIRLGEITLDTE
VTVSANAAAEPPSRLGLSAGTTIRLRYLLRAAAIRSGNDAATAIGEAISGSEAAFARRMTRTARALGMTRTTFRNAHGLT
EDGHLSTARDMTMLGRRLVYDYPQYYNLFSRVTADAGIATIRNTNRVVLTTYRGADGIKTGYTRAAGFNLVASAQRGEER
IIATVFGGRSSAWRNQRVMELLDMGFERAPSHVALRTPPLPTYADDGSPVGRTATPPGQRAPERSMRPISRPGGGGAGVD
AEVLAAISEAVGAALQPGGDVAADVEAALSFVARPTDRPAPRPEPQVAAEPEPAPIPQPEPELVTRASSTGSRHFGVSLG
LQPSHHAAERLLLRTALAELGTFDTALREVRNVTRGFDARFAGMTEDQASRACARLQARDVACETFGP

Sequences:

>Translated_468_residues
MFVRNLFVALLGVQILFGAVAPAQVNAAPYAAMVMDARNGEVLHSRNADTRLHPASLTKMMTLYIAFEAIRLGEITLDTE
VTVSANAAAEPPSRLGLSAGTTIRLRYLLRAAAIRSGNDAATAIGEAISGSEAAFARRMTRTARALGMTRTTFRNAHGLT
EDGHLSTARDMTMLGRRLVYDYPQYYNLFSRVTADAGIATIRNTNRVVLTTYRGADGIKTGYTRAAGFNLVASAQRGEER
IIATVFGGRSSAWRNQRVMELLDMGFERAPSHVALRTPPLPTYADDGSPVGRTATPPGQRAPERSMRPISRPGGGGAGVD
AEVLAAISEAVGAALQPGGDVAADVEAALSFVARPTDRPAPRPEPQVAAEPEPAPIPQPEPELVTRASSTGSRHFGVSLG
LQPSHHAAERLLLRTALAELGTFDTALREVRNVTRGFDARFAGMTEDQASRACARLQARDVACETFGP
>Mature_468_residues
MFVRNLFVALLGVQILFGAVAPAQVNAAPYAAMVMDARNGEVLHSRNADTRLHPASLTKMMTLYIAFEAIRLGEITLDTE
VTVSANAAAEPPSRLGLSAGTTIRLRYLLRAAAIRSGNDAATAIGEAISGSEAAFARRMTRTARALGMTRTTFRNAHGLT
EDGHLSTARDMTMLGRRLVYDYPQYYNLFSRVTADAGIATIRNTNRVVLTTYRGADGIKTGYTRAAGFNLVASAQRGEER
IIATVFGGRSSAWRNQRVMELLDMGFERAPSHVALRTPPLPTYADDGSPVGRTATPPGQRAPERSMRPISRPGGGGAGVD
AEVLAAISEAVGAALQPGGDVAADVEAALSFVARPTDRPAPRPEPQVAAEPEPAPIPQPEPELVTRASSTGSRHFGVSLG
LQPSHHAAERLLLRTALAELGTFDTALREVRNVTRGFDARFAGMTEDQASRACARLQARDVACETFGP

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=244, Percent_Identity=38.5245901639344, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1786851, Length=251, Percent_Identity=36.6533864541833, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082022, Length=271, Percent_Identity=33.9483394833948, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082054, Length=213, Percent_Identity=30.0469483568075, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49873; Mature: 49873

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVRNLFVALLGVQILFGAVAPAQVNAAPYAAMVMDARNGEVLHSRNADTRLHPASLTKM
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHH
MTLYIAFEAIRLGEITLDTEVTVSANAAAEPPSRLGLSAGTTIRLRYLLRAAAIRSGNDA
HHHHHHHHHHEECEEEECCEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCHH
ATAIGEAISGSEAAFARRMTRTARALGMTRTTFRNAHGLTEDGHLSTARDMTMLGRRLVY
HHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
DYPQYYNLFSRVTADAGIATIRNTNRVVLTTYRGADGIKTGYTRAAGFNLVASAQRGEER
CCHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCCHHCCCCEEECCCCCCCE
IIATVFGGRSSAWRNQRVMELLDMGFERAPSHVALRTPPLPTYADDGSPVGRTATPPGQR
EEEEEECCCCCHHHHHHHHHHHHCCHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
APERSMRPISRPGGGGAGVDAEVLAAISEAVGAALQPGGDVAADVEAALSFVARPTDRPA
CCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
PRPEPQVAAEPEPAPIPQPEPELVTRASSTGSRHFGVSLGLQPSHHAAERLLLRTALAEL
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
GTFDTALREVRNVTRGFDARFAGMTEDQASRACARLQARDVACETFGP
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFVRNLFVALLGVQILFGAVAPAQVNAAPYAAMVMDARNGEVLHSRNADTRLHPASLTKM
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCHHHHHHH
MTLYIAFEAIRLGEITLDTEVTVSANAAAEPPSRLGLSAGTTIRLRYLLRAAAIRSGNDA
HHHHHHHHHHEECEEEECCEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCHH
ATAIGEAISGSEAAFARRMTRTARALGMTRTTFRNAHGLTEDGHLSTARDMTMLGRRLVY
HHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
DYPQYYNLFSRVTADAGIATIRNTNRVVLTTYRGADGIKTGYTRAAGFNLVASAQRGEER
CCHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCCHHCCCCEEECCCCCCCE
IIATVFGGRSSAWRNQRVMELLDMGFERAPSHVALRTPPLPTYADDGSPVGRTATPPGQR
EEEEEECCCCCHHHHHHHHHHHHCCHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
APERSMRPISRPGGGGAGVDAEVLAAISEAVGAALQPGGDVAADVEAALSFVARPTDRPA
CCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
PRPEPQVAAEPEPAPIPQPEPELVTRASSTGSRHFGVSLGLQPSHHAAERLLLRTALAEL
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
GTFDTALREVRNVTRGFDARFAGMTEDQASRACARLQARDVACETFGP
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]