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The map label for this gene is caiD [H]

Identifier: 89056315

GI number: 89056315

Start: 3916807

End: 3917601

Strand: Direct

Name: caiD [H]

Synonym: Jann_3824

Alternate gene names: 89056315

Gene position: 3916807-3917601 (Clockwise)

Preceding gene: 89056314

Following gene: 89056316

Centisome position: 90.71

GC content: 62.39

Gene sequence:

>795_bases
ATGACACCCGCCGAAGACAACCCAATCCAGACCGCCCGCCGGGGCCATGTGCTGGAAGTCACACTGGACCGCGGCAAAGC
CAATGCCATCGATCTCGCGACCAGCCGGATCATGGGCGATATCTTCACAGAGTTTCGCGATGACCCGGAGCTTCGTGTCG
CCATCGTGACCGGCGCGGGTGAGAAGTTCTTTTGCCCCGGCTGGGACCTGAAAGCCGCCGCCGACGGTGATGCGGTGGAC
GGGGATTACGGGGTCGGCGGTTTTGGCGGGCTGCAAGAGCTGCGCGGGCTCAACAAGCCTGTGATCGCGGCAGTGAACGG
GATCTGCTGCGGAGGCGGGCTGGAACTGGCGCTGTCCGCCGACATTATCCTGGCCGCCGATCATGCGACTTTTGCCTTGC
CAGAGATACGGTCCGGCACTGTGGCTGATGCGGCCTCCATCAAGCTGCCCAAGCGCATTCCTTACCACATCGCGATGGAG
ATGCTGTTCACCGGGCGCTGGATCGATGCGGAGGAGGCTGCCCGTTGGGGGATGATCAACCGGATCATCCCGGCCGCGCA
ATTGATGGATCAAGCCCGTGAGATGGCGGATCTGTTGGCGAGCGGTCCGCCGCTGGTCTACGCCGCGATCAAAGAGGTTG
TCAGAGAGGCCGAGGACATGAAGTTTCAGGACGCGCTGAACAAGATCACCAAAAGCCAGTTTGAGACGGTTGAAGCGCTC
TACCGCTCGGAGGATCAACTGGAAGGCGCGCGCGCCTTTGCCGAGAAACGCGCGCCGGTCTGGCAAGGCAAGTGA

Upstream 100 bases:

>100_bases
CCAACGCGCCGGATATCTTTGAGGTCGAGATCAACCCGCTTCTCTGCACACCCACCCACGCCGTCGCCGCCGACGCCCTC
ATCCGCCTAAGGACCACTCC

Downstream 100 bases:

>100_bases
CCACGATATGGGCCACGACTTGGGGGACGGCGGGTGGGGCGAGGTTGACGGTTTTGCCCCGCAAAATCGTTGACCAGCGT
CACCCCCGGCCAAAAAAGGA

Product: carnitinyl-CoA dehydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTPAEDNPIQTARRGHVLEVTLDRGKANAIDLATSRIMGDIFTEFRDDPELRVAIVTGAGEKFFCPGWDLKAAADGDAVD
GDYGVGGFGGLQELRGLNKPVIAAVNGICCGGGLELALSADIILAADHATFALPEIRSGTVADAASIKLPKRIPYHIAME
MLFTGRWIDAEEAARWGMINRIIPAAQLMDQAREMADLLASGPPLVYAAIKEVVREAEDMKFQDALNKITKSQFETVEAL
YRSEDQLEGARAFAEKRAPVWQGK

Sequences:

>Translated_264_residues
MTPAEDNPIQTARRGHVLEVTLDRGKANAIDLATSRIMGDIFTEFRDDPELRVAIVTGAGEKFFCPGWDLKAAADGDAVD
GDYGVGGFGGLQELRGLNKPVIAAVNGICCGGGLELALSADIILAADHATFALPEIRSGTVADAASIKLPKRIPYHIAME
MLFTGRWIDAEEAARWGMINRIIPAAQLMDQAREMADLLASGPPLVYAAIKEVVREAEDMKFQDALNKITKSQFETVEAL
YRSEDQLEGARAFAEKRAPVWQGK
>Mature_263_residues
TPAEDNPIQTARRGHVLEVTLDRGKANAIDLATSRIMGDIFTEFRDDPELRVAIVTGAGEKFFCPGWDLKAAADGDAVDG
DYGVGGFGGLQELRGLNKPVIAAVNGICCGGGLELALSADIILAADHATFALPEIRSGTVADAASIKLPKRIPYHIAMEM
LFTGRWIDAEEAARWGMINRIIPAAQLMDQAREMADLLASGPPLVYAAIKEVVREAEDMKFQDALNKITKSQFETVEALY
RSEDQLEGARAFAEKRAPVWQGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=240, Percent_Identity=35, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=28.8065843621399, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=30.0380228136882, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=54.4747081712062, Blast_Score=281, Evalue=2e-77,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082183, Length=229, Percent_Identity=30.5676855895196, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=28.6290322580645, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=31.3725490196078, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788682, Length=165, Percent_Identity=30.3030303030303, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=32.046332046332, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=29.2490118577075, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=229, Percent_Identity=28.82096069869, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=268, Percent_Identity=28.3582089552239, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=185, Percent_Identity=28.1081081081081, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=166, Percent_Identity=28.9156626506024, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=33.7448559670782, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=33.7448559670782, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=30.1526717557252, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=32.4175824175824, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=30.2564102564103, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=23.4817813765182, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28572; Mature: 28441

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAEDNPIQTARRGHVLEVTLDRGKANAIDLATSRIMGDIFTEFRDDPELRVAIVTGAG
CCCCCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
EKFFCPGWDLKAAADGDAVDGDYGVGGFGGLQELRGLNKPVIAAVNGICCGGGLELALSA
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCEEECCCCEEEEEC
DIILAADHATFALPEIRSGTVADAASIKLPKRIPYHIAMEMLFTGRWIDAEEAARWGMIN
CEEEEECCCEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RIIPAAQLMDQAREMADLLASGPPLVYAAIKEVVREAEDMKFQDALNKITKSQFETVEAL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YRSEDQLEGARAFAEKRAPVWQGK
HCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TPAEDNPIQTARRGHVLEVTLDRGKANAIDLATSRIMGDIFTEFRDDPELRVAIVTGAG
CCCCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
EKFFCPGWDLKAAADGDAVDGDYGVGGFGGLQELRGLNKPVIAAVNGICCGGGLELALSA
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCEEECCCCEEEEEC
DIILAADHATFALPEIRSGTVADAASIKLPKRIPYHIAMEMLFTGRWIDAEEAARWGMIN
CEEEEECCCEEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RIIPAAQLMDQAREMADLLASGPPLVYAAIKEVVREAEDMKFQDALNKITKSQFETVEAL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YRSEDQLEGARAFAEKRAPVWQGK
HCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA