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The map label for this gene is hyuA [H]

Identifier: 89056256

GI number: 89056256

Start: 3847193

End: 3849265

Strand: Direct

Name: hyuA [H]

Synonym: Jann_3765

Alternate gene names: 89056256

Gene position: 3847193-3849265 (Clockwise)

Preceding gene: 89056255

Following gene: 89056257

Centisome position: 89.1

GC content: 65.99

Gene sequence:

>2073_bases
ATGAGCCGCATGATCGGCGTCGATGTTGGCGGCACATTCACCGATGTCTTCGTACTTGATGAGGACAGCGGCGTGGCCGA
GGTCGCCAAGGTGCCGACGACGCGCCCGGATCAGTCCGGCGGTTTTCTCGACGGCATCGCGCAGCGGGTGGACGATTTGT
CGGGCGTCGATGTGGTCGTGCATGGCACGACGGCGGGCACAAACGCCCTGCTGGAGCGCAAGGGCGCGAAGATCGGCGTG
ATCACCACAGAAGGCCTGCGCGACGTGCTGGAAATGCGCCGCCGGGACCGCCCGCGCACATGGGGGTTGCGCGGAGATTT
CGACCCCGTGGTGCCGCGCGATCTGCGGCTTGAAGTGCCCGAGCGCACGCTGGCCGATGGGACGATCCGAACGCCCGTGG
ACCTGGACGCCGTGCAGGAGGCGGCACGGGCGCTGATCGCGGCAGGCTGTGAGGCGGTGGCGATCCTCTTTGCCAACGCC
TATGCCAACCCCGAAAACGAGGCCCGCGCGACCGAGGCGATCCGCGCGATCTGGCCCAATCCGCATGTCAGCTGCTCCTC
GGAAATCCTGCCGGAAATCCGCGAGTTCGAACGGTTCTCGACCACGGCCCTCAACGCCTATCTGCAACCGGAGGTCTCCG
GCTATATCGACCGGCTCGACCGCGCGCTCACGGATGGCGGCTTCGGCGGAGAGTTCATGATCGTGCAGTCCAATGGCGGT
GTCATGGGCACCGATACCGCCTGCCGCCTGCCCGTGCGCACGGCGCTGTCGGGGCCAGCTGCCGGGGTCATTGCCGCCGG
TCACATCGCCCGCGTGGCGGGGTTCGAGAACGTTGTCACCGGCGACATGGGCGGCACCAGTTTCGATGTGGCCCTGATCG
CTGAAGGCCAATCCCAGATCGCCGCCCAGAACGCCGTGGATTTCGGCATGGTCGTGCGCACCCCGATGATCGAGATCACG
ACGATCGGAGCGGGTGGCGGGTCCATCGCCTGGGTCGACAAGGGTGGCCTTCTGAATATCGGCCCCGAAAGTGCAGGCTC
CACCCCCGGCCCCGTGGCCTATGGGCAGGGCAACACACGCCCCACGGTGACGGACGCGAATGTGGTGCTGGGTCGGATTG
ACCCGGACAACCCCATCGGCGGCAAGCTGGATCGGTTGGACGTGGAAGCCGCTGGCCGCGCCATCGACGCGGAGGTGGGC
GGGGAACTTGGCCTGACTACGACCCAGGCCGCCGAGGCGATCCTGACTGTCGCCAATTCCCGCATGGCGGGGGCGATCCG
GTTGGTCTCTATCGAGCGGGGCTTTGATCCGAAGCGTTTTGCCTTCATGCCGTTTGGCGGCGGCGGTGCGCTCCATACCG
GCGCGATGCTGCGCGAGGTTGGCATCGGCTCTGCCATCGTGCCGCGATATCCCGGCGTGACCTCGGCGATGGGCTGCGTG
ATTGCCGACATGCGGCAGGATTTCGTGCAGACGATCAACGCTCTGACCGCCACGCTCGACACTGATATGTTGCGGACGGT
CATGCAGCGCCATGTCGACGACGGGCTGGCGCTTCTGGAGGCCGCGAAATCCCGGTTCGACGCACGCGATGTGCTGTTTG
AACTGGACATGGCCTATATCGGGCAGACCCACACGGTCTCGGTGCCGGTGGAGATCGCTGTGCGAAACGGCACCGTGGTC
GCGCCCAGTATCGCCGATCTCGAAGAGGCCTTCGATACGGCCTATAAGGCGACGTTCGGCAGGCTTCTGAAGGCCGGAAC
ACGGCGCGTTATCAACCTGCGGACGACGGTGATTGGACGGCGCTCGAAGTTTGACCTGACAACACTCGCGCCCGGCGCGG
GGAGCATGGCGGACGCCCAAACTGGCACACGACGGGTCCATTTCGGGGATAGCTGGCATGAGACCGCGATTTACGACCGT
TTGGCCCTGCCAGTGGGGGCTGAAATCCGCGGCCCGGCCATCCTTGTTCAACCGGATACAACCGTGCTGGTCGACCCGGG
GCTGACCGGCCGCGTGGACAGGTTCGGCAACACAATCATCACGCCCGACGCGGCACCAGAGAGCGGGGGCTGA

Upstream 100 bases:

>100_bases
ACCGTTCGCCCGAAGCCGTGGCGCGCGATGTGGCGCTTGGCTACCTGAGTGCTGAGGCGGCGACCGAAAGCTACGGGTCG
GCCTGGCAGGAGTGCGCATC

Downstream 100 bases:

>100_bases
CATGATCACCCATATTGATCCGAAACGGACGGCACTTCTGACCATCGACCTGCAAAACGATTTCCTGCACCCCGAAGGCG
CCTACGGCCGCGCCGGGCAG

Product: 5-oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSRMIGVDVGGTFTDVFVLDEDSGVAEVAKVPTTRPDQSGGFLDGIAQRVDDLSGVDVVVHGTTAGTNALLERKGAKIGV
ITTEGLRDVLEMRRRDRPRTWGLRGDFDPVVPRDLRLEVPERTLADGTIRTPVDLDAVQEAARALIAAGCEAVAILFANA
YANPENEARATEAIRAIWPNPHVSCSSEILPEIREFERFSTTALNAYLQPEVSGYIDRLDRALTDGGFGGEFMIVQSNGG
VMGTDTACRLPVRTALSGPAAGVIAAGHIARVAGFENVVTGDMGGTSFDVALIAEGQSQIAAQNAVDFGMVVRTPMIEIT
TIGAGGGSIAWVDKGGLLNIGPESAGSTPGPVAYGQGNTRPTVTDANVVLGRIDPDNPIGGKLDRLDVEAAGRAIDAEVG
GELGLTTTQAAEAILTVANSRMAGAIRLVSIERGFDPKRFAFMPFGGGGALHTGAMLREVGIGSAIVPRYPGVTSAMGCV
IADMRQDFVQTINALTATLDTDMLRTVMQRHVDDGLALLEAAKSRFDARDVLFELDMAYIGQTHTVSVPVEIAVRNGTVV
APSIADLEEAFDTAYKATFGRLLKAGTRRVINLRTTVIGRRSKFDLTTLAPGAGSMADAQTGTRRVHFGDSWHETAIYDR
LALPVGAEIRGPAILVQPDTTVLVDPGLTGRVDRFGNTIITPDAAPESGG

Sequences:

>Translated_690_residues
MSRMIGVDVGGTFTDVFVLDEDSGVAEVAKVPTTRPDQSGGFLDGIAQRVDDLSGVDVVVHGTTAGTNALLERKGAKIGV
ITTEGLRDVLEMRRRDRPRTWGLRGDFDPVVPRDLRLEVPERTLADGTIRTPVDLDAVQEAARALIAAGCEAVAILFANA
YANPENEARATEAIRAIWPNPHVSCSSEILPEIREFERFSTTALNAYLQPEVSGYIDRLDRALTDGGFGGEFMIVQSNGG
VMGTDTACRLPVRTALSGPAAGVIAAGHIARVAGFENVVTGDMGGTSFDVALIAEGQSQIAAQNAVDFGMVVRTPMIEIT
TIGAGGGSIAWVDKGGLLNIGPESAGSTPGPVAYGQGNTRPTVTDANVVLGRIDPDNPIGGKLDRLDVEAAGRAIDAEVG
GELGLTTTQAAEAILTVANSRMAGAIRLVSIERGFDPKRFAFMPFGGGGALHTGAMLREVGIGSAIVPRYPGVTSAMGCV
IADMRQDFVQTINALTATLDTDMLRTVMQRHVDDGLALLEAAKSRFDARDVLFELDMAYIGQTHTVSVPVEIAVRNGTVV
APSIADLEEAFDTAYKATFGRLLKAGTRRVINLRTTVIGRRSKFDLTTLAPGAGSMADAQTGTRRVHFGDSWHETAIYDR
LALPVGAEIRGPAILVQPDTTVLVDPGLTGRVDRFGNTIITPDAAPESGG
>Mature_689_residues
SRMIGVDVGGTFTDVFVLDEDSGVAEVAKVPTTRPDQSGGFLDGIAQRVDDLSGVDVVVHGTTAGTNALLERKGAKIGVI
TTEGLRDVLEMRRRDRPRTWGLRGDFDPVVPRDLRLEVPERTLADGTIRTPVDLDAVQEAARALIAAGCEAVAILFANAY
ANPENEARATEAIRAIWPNPHVSCSSEILPEIREFERFSTTALNAYLQPEVSGYIDRLDRALTDGGFGGEFMIVQSNGGV
MGTDTACRLPVRTALSGPAAGVIAAGHIARVAGFENVVTGDMGGTSFDVALIAEGQSQIAAQNAVDFGMVVRTPMIEITT
IGAGGGSIAWVDKGGLLNIGPESAGSTPGPVAYGQGNTRPTVTDANVVLGRIDPDNPIGGKLDRLDVEAAGRAIDAEVGG
ELGLTTTQAAEAILTVANSRMAGAIRLVSIERGFDPKRFAFMPFGGGGALHTGAMLREVGIGSAIVPRYPGVTSAMGCVI
ADMRQDFVQTINALTATLDTDMLRTVMQRHVDDGLALLEAAKSRFDARDVLFELDMAYIGQTHTVSVPVEIAVRNGTVVA
PSIADLEEAFDTAYKATFGRLLKAGTRRVINLRTTVIGRRSKFDLTTLAPGAGSMADAQTGTRRVHFGDSWHETAIYDRL
ALPVGAEIRGPAILVQPDTTVLVDPGLTGRVDRFGNTIITPDAAPESGG

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=741, Percent_Identity=30.3643724696356, Blast_Score=212, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI133901900, Length=718, Percent_Identity=26.0445682451253, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322634, Length=744, Percent_Identity=27.4193548387097, Blast_Score=210, Evalue=8e-55,
Organism=Drosophila melanogaster, GI45550492, Length=732, Percent_Identity=27.5956284153005, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 72641; Mature: 72510

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMIGVDVGGTFTDVFVLDEDSGVAEVAKVPTTRPDQSGGFLDGIAQRVDDLSGVDVVV
CCCEEEEECCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
HGTTAGTNALLERKGAKIGVITTEGLRDVLEMRRRDRPRTWGLRGDFDPVVPRDLRLEVP
ECCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECC
ERTLADGTIRTPVDLDAVQEAARALIAAGCEAVAILFANAYANPENEARATEAIRAIWPN
CHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHEEHHHHHHCCCCCHHHHHHHHHHHHCCC
PHVSCSSEILPEIREFERFSTTALNAYLQPEVSGYIDRLDRALTDGGFGGEFMIVQSNGG
CCCCCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
VMGTDTACRLPVRTALSGPAAGVIAAGHIARVAGFENVVTGDMGGTSFDVALIAEGQSQI
EEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCCHHH
AAQNAVDFGMVVRTPMIEITTIGAGGGSIAWVDKGGLLNIGPESAGSTPGPVAYGQGNTR
HHHCCCCCCEEEECCEEEEEEEECCCCEEEEEECCCEEEECCCCCCCCCCCEEECCCCCC
PTVTDANVVLGRIDPDNPIGGKLDRLDVEAAGRAIDAEVGGELGLTTTQAAEAILTVANS
CEEEECCEEEEECCCCCCCCCCCCEEEHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
RMAGAIRLVSIERGFDPKRFAFMPFGGGGALHTGAMLREVGIGSAIVPRYPGVTSAMGCV
CCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHH
IADMRQDFVQTINALTATLDTDMLRTVMQRHVDDGLALLEAAKSRFDARDVLFELDMAYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GQTHTVSVPVEIAVRNGTVVAPSIADLEEAFDTAYKATFGRLLKAGTRRVINLRTTVIGR
CCCEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHEECC
RSKFDLTTLAPGAGSMADAQTGTRRVHFGDSWHETAIYDRLALPVGAEIRGPAILVQPDT
CCCCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCCC
TVLVDPGLTGRVDRFGNTIITPDAAPESGG
EEEECCCCCCCHHHCCCEEECCCCCCCCCC
>Mature Secondary Structure 
SRMIGVDVGGTFTDVFVLDEDSGVAEVAKVPTTRPDQSGGFLDGIAQRVDDLSGVDVVV
CCEEEEECCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
HGTTAGTNALLERKGAKIGVITTEGLRDVLEMRRRDRPRTWGLRGDFDPVVPRDLRLEVP
ECCCCCCHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECC
ERTLADGTIRTPVDLDAVQEAARALIAAGCEAVAILFANAYANPENEARATEAIRAIWPN
CHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHEEHHHHHHCCCCCHHHHHHHHHHHHCCC
PHVSCSSEILPEIREFERFSTTALNAYLQPEVSGYIDRLDRALTDGGFGGEFMIVQSNGG
CCCCCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
VMGTDTACRLPVRTALSGPAAGVIAAGHIARVAGFENVVTGDMGGTSFDVALIAEGQSQI
EEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCCHHH
AAQNAVDFGMVVRTPMIEITTIGAGGGSIAWVDKGGLLNIGPESAGSTPGPVAYGQGNTR
HHHCCCCCCEEEECCEEEEEEEECCCCEEEEEECCCEEEECCCCCCCCCCCEEECCCCCC
PTVTDANVVLGRIDPDNPIGGKLDRLDVEAAGRAIDAEVGGELGLTTTQAAEAILTVANS
CEEEECCEEEEECCCCCCCCCCCCEEEHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCC
RMAGAIRLVSIERGFDPKRFAFMPFGGGGALHTGAMLREVGIGSAIVPRYPGVTSAMGCV
CCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHH
IADMRQDFVQTINALTATLDTDMLRTVMQRHVDDGLALLEAAKSRFDARDVLFELDMAYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GQTHTVSVPVEIAVRNGTVVAPSIADLEEAFDTAYKATFGRLLKAGTRRVINLRTTVIGR
CCCEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHEECC
RSKFDLTTLAPGAGSMADAQTGTRRVHFGDSWHETAIYDRLALPVGAEIRGPAILVQPDT
CCCCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCCC
TVLVDPGLTGRVDRFGNTIITPDAAPESGG
EEEECCCCCCCHHHCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]