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The map label for this gene is gcvH [H]

Identifier: 89056112

GI number: 89056112

Start: 3693349

End: 3693711

Strand: Direct

Name: gcvH [H]

Synonym: Jann_3621

Alternate gene names: 89056112

Gene position: 3693349-3693711 (Clockwise)

Preceding gene: 89056111

Following gene: 89056113

Centisome position: 85.53

GC content: 57.58

Gene sequence:

>363_bases
ATGAAATTCACTGAAGACCACGAATGGCTGAACGCCGACGGCGACGTTATCACTGTTGGCATCACCGAACACGCCGCCAC
GCAACTGGGCGATGTCGTCTATGTCGAGTTGCCCGAGGTGGAAACCAAAGTGTCCAAGGGTGACGAAATTGTGGTGATCG
AGAGTGTCAAAGCCGCTTCCGACATCATCGCGCCGCTGGATGGTGAAATCGTGGAGGTCAACGACGCGCTCGCCGACAAA
CCCGCCCTCGTCAACGAGGACGCCATGGGCGCGGCCTGGTTCTTCAAGATCAAGGTCGAGAGCGCCGACGCGTTGGACGA
CTACATGTCCGAGGACGAATACAAGGATCTCGTCGGGGACTAA

Upstream 100 bases:

>100_bases
TGACGTGCGCGGCAAACGCCTGCCCGTCGCACAGACCAAACTTCCTTTCACACCCCCCGGCTACAAACGCTGACATCGAC
GAAACACGACGGAGACACCG

Downstream 100 bases:

>100_bases
GGGGCCAGCCTCCAACCGGGGGCATTACTGCCCAATTTCCCGCGGTTTTTTTGACAAGATGACAGCGCAAGACCTCAGAC
AGGTATTGCCGATGGAGTGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MKFTEDHEWLNADGDVITVGITEHAATQLGDVVYVELPEVETKVSKGDEIVVIESVKAASDIIAPLDGEIVEVNDALADK
PALVNEDAMGAAWFFKIKVESADALDDYMSEDEYKDLVGD

Sequences:

>Translated_120_residues
MKFTEDHEWLNADGDVITVGITEHAATQLGDVVYVELPEVETKVSKGDEIVVIESVKAASDIIAPLDGEIVEVNDALADK
PALVNEDAMGAAWFFKIKVESADALDDYMSEDEYKDLVGD
>Mature_120_residues
MKFTEDHEWLNADGDVITVGITEHAATQLGDVVYVELPEVETKVSKGDEIVVIESVKAASDIIAPLDGEIVEVNDALADK
PALVNEDAMGAAWFFKIKVESADALDDYMSEDEYKDLVGD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=47.008547008547, Blast_Score=119, Evalue=4e-28,
Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=47.008547008547, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=51.2396694214876, Blast_Score=119, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=41.7391304347826, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=44.0677966101695, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=43.2, Blast_Score=100, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=38.3333333333333, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13059; Mature: 13059

Theoretical pI: Translated: 3.72; Mature: 3.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTEDHEWLNADGDVITVGITEHAATQLGDVVYVELPEVETKVSKGDEIVVIESVKAAS
CCCCCCCCEECCCCCEEEEEECHHHHHHCCCEEEEECCCCHHHCCCCCCEEEEECCCCCH
DIIAPLDGEIVEVNDALADKPALVNEDAMGAAWFFKIKVESADALDDYMSEDEYKDLVGD
HCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MKFTEDHEWLNADGDVITVGITEHAATQLGDVVYVELPEVETKVSKGDEIVVIESVKAAS
CCCCCCCCEECCCCCEEEEEECHHHHHHCCCEEEEECCCCHHHCCCCCCEEEEECCCCCH
DIIAPLDGEIVEVNDALADKPALVNEDAMGAAWFFKIKVESADALDDYMSEDEYKDLVGD
HCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]