Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 89056043

GI number: 89056043

Start: 3615322

End: 3616473

Strand: Direct

Name: mdeA [H]

Synonym: Jann_3552

Alternate gene names: 89056043

Gene position: 3615322-3616473 (Clockwise)

Preceding gene: 89056042

Following gene: 89056044

Centisome position: 83.73

GC content: 61.89

Gene sequence:

>1152_bases
ATGTCTGATGGACCGGACATTGCGACCGTCCTCGCGTCGGTGCTGGCCGACGATGGTGGGTCCATGACGCCGCCAATCGT
GCAGACGTCCCTGTTCGCCTTCGACAACTACGCGGCGTTTGAGGCGCGCATGGCCGGGGACACCGACACCGCCATCTATA
CCCGCGTGCAAAATCCCACCGTTGCGGCGTTCGAAGGGTTGATGGCGCAGGCCGAAGAGGGAGAGGCCGCTGTAGGCTTC
GCCTCGGGCATGGCGGCGATCAGCTCCACGCTTCTGGCCTTCGTCAAACCCGGCGACCGGATTGCCTGCATCGAACACGC
CTATCCCGACACCTACCGTTTTTTTGAACGGATGCTGCGGCCCTTTGGTGTCCAGATCGATTACCACCCCGCCCGCGCGT
TTGAGGATGACCCCGATCTGCTTAACGGGGTTGCCATTGCCTATCTGGAAAGTCCCAGCTCGGTCGTTTTCACGCCGATG
AATTTGGCCCGTGTGGCAGAGCACGCAACGCGCCACGGCACAGTGACAATGATCGACAATTCCTGGGCCAGCCCGGTGTT
CCAACGCCCCCTCACGCTGGGGATCGACATCGTGCTGCACTCGGCCTCGAAGTACATTTCGGGCCACTCAGACACGGTTG
CGGGCGTGGTGGTGTCCTCCCACGCCCATATCGCCCGCATCCGTGATCTGACCTTGCCGCTTCTGGGCGCGAAACTTGCG
CCGTTTGAAGCCTTTCTTCTCACCCGTGGCCTGCGCACCTTGAAGGTGCGGATGATGCAGCATCAGGCGACGGCGGATCT
GTTCGTGGACCGGTTGGCTGCGGTGCCACAGGTGCGCAGGATCAACAGCCCCGGCGCCAACGCGGTGCCCGGCCTGACGG
GCCGCTCCGGTCTGATGTCCATCGAATTCGACGACACCGTTGATATCCCAGCATTTGCCGACGCGTTCACTCATTTCCGG
CTTGGCGTCAGCTGGGGCGGTTTTGAAAGTCTCGTCCTGCCGGCGCGCGTCGCCTTGGCGCAGGCGGGCGAGCACAATTC
ACTGCAAGCGTTCGGCGTATCCCCAAATCTGGTGCGCCTGAACCTTGGCTTGGAAGATCCAGAAGACCTTTGGGCAGATT
TTGAATCTGCCCTGAGTGCAAGTCTGACCTGA

Upstream 100 bases:

>100_bases
GTTGCGGGGGACGCTGATAAAGCCGCAGCGCTCATGGATGAAATCTTGTCGATGGTGGAAGATGAAATCCGGCAGGTGAT
GCAAGACATGGGTGCGCGTG

Downstream 100 bases:

>100_bases
CAAACCGGGTCCGCATACGACAACACAGGAAGAGAAGGGAGTTGCTTATGAAACGTATTCTTATCAGTGCCAGCACCATC
GCCATGATGGTCGCCGGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSDGPDIATVLASVLADDGGSMTPPIVQTSLFAFDNYAAFEARMAGDTDTAIYTRVQNPTVAAFEGLMAQAEEGEAAVGF
ASGMAAISSTLLAFVKPGDRIACIEHAYPDTYRFFERMLRPFGVQIDYHPARAFEDDPDLLNGVAIAYLESPSSVVFTPM
NLARVAEHATRHGTVTMIDNSWASPVFQRPLTLGIDIVLHSASKYISGHSDTVAGVVVSSHAHIARIRDLTLPLLGAKLA
PFEAFLLTRGLRTLKVRMMQHQATADLFVDRLAAVPQVRRINSPGANAVPGLTGRSGLMSIEFDDTVDIPAFADAFTHFR
LGVSWGGFESLVLPARVALAQAGEHNSLQAFGVSPNLVRLNLGLEDPEDLWADFESALSASLT

Sequences:

>Translated_383_residues
MSDGPDIATVLASVLADDGGSMTPPIVQTSLFAFDNYAAFEARMAGDTDTAIYTRVQNPTVAAFEGLMAQAEEGEAAVGF
ASGMAAISSTLLAFVKPGDRIACIEHAYPDTYRFFERMLRPFGVQIDYHPARAFEDDPDLLNGVAIAYLESPSSVVFTPM
NLARVAEHATRHGTVTMIDNSWASPVFQRPLTLGIDIVLHSASKYISGHSDTVAGVVVSSHAHIARIRDLTLPLLGAKLA
PFEAFLLTRGLRTLKVRMMQHQATADLFVDRLAAVPQVRRINSPGANAVPGLTGRSGLMSIEFDDTVDIPAFADAFTHFR
LGVSWGGFESLVLPARVALAQAGEHNSLQAFGVSPNLVRLNLGLEDPEDLWADFESALSASLT
>Mature_382_residues
SDGPDIATVLASVLADDGGSMTPPIVQTSLFAFDNYAAFEARMAGDTDTAIYTRVQNPTVAAFEGLMAQAEEGEAAVGFA
SGMAAISSTLLAFVKPGDRIACIEHAYPDTYRFFERMLRPFGVQIDYHPARAFEDDPDLLNGVAIAYLESPSSVVFTPMN
LARVAEHATRHGTVTMIDNSWASPVFQRPLTLGIDIVLHSASKYISGHSDTVAGVVVSSHAHIARIRDLTLPLLGAKLAP
FEAFLLTRGLRTLKVRMMQHQATADLFVDRLAAVPQVRRINSPGANAVPGLTGRSGLMSIEFDDTVDIPAFADAFTHFRL
GVSWGGFESLVLPARVALAQAGEHNSLQAFGVSPNLVRLNLGLEDPEDLWADFESALSASLT

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=358, Percent_Identity=31.0055865921788, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI25453487, Length=356, Percent_Identity=29.2134831460674, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI299473759, Length=275, Percent_Identity=31.2727272727273, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1790375, Length=386, Percent_Identity=32.6424870466321, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789383, Length=384, Percent_Identity=30.2083333333333, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17538055, Length=364, Percent_Identity=30.2197802197802, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560056, Length=364, Percent_Identity=29.3956043956044, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=29.8387096774194, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319307, Length=381, Percent_Identity=29.6587926509186, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=27.6923076923077, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=399, Percent_Identity=25.8145363408521, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323333, Length=414, Percent_Identity=25.8454106280193, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321254, Length=397, Percent_Identity=25.6926952141058, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922554, Length=370, Percent_Identity=27.8378378378378, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 41089; Mature: 40958

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGPDIATVLASVLADDGGSMTPPIVQTSLFAFDNYAAFEARMAGDTDTAIYTRVQNPT
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHEEEECCCCCCEEEEEECCCH
VAAFEGLMAQAEEGEAAVGFASGMAAISSTLLAFVKPGDRIACIEHAYPDTYRFFERMLR
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
PFGVQIDYHPARAFEDDPDLLNGVAIAYLESPSSVVFTPMNLARVAEHATRHGTVTMIDN
HHCCEEEECCCCCCCCCHHHHCCEEEEEEECCCCEEEECHHHHHHHHHHHCCCEEEEECC
SWASPVFQRPLTLGIDIVLHSASKYISGHSDTVAGVVVSSHAHIARIRDLTLPLLGAKLA
CCCCHHHHCCHHHHHHHEEECHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCC
PFEAFLLTRGLRTLKVRMMQHQATADLFVDRLAAVPQVRRINSPGANAVPGLTGRSGLMS
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEE
IEFDDTVDIPAFADAFTHFRLGVSWGGFESLVLPARVALAQAGEHNSLQAFGVSPNLVRL
EECCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEE
NLGLEDPEDLWADFESALSASLT
EECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDGPDIATVLASVLADDGGSMTPPIVQTSLFAFDNYAAFEARMAGDTDTAIYTRVQNPT
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHEEEECCCCCCEEEEEECCCH
VAAFEGLMAQAEEGEAAVGFASGMAAISSTLLAFVKPGDRIACIEHAYPDTYRFFERMLR
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
PFGVQIDYHPARAFEDDPDLLNGVAIAYLESPSSVVFTPMNLARVAEHATRHGTVTMIDN
HHCCEEEECCCCCCCCCHHHHCCEEEEEEECCCCEEEECHHHHHHHHHHHCCCEEEEECC
SWASPVFQRPLTLGIDIVLHSASKYISGHSDTVAGVVVSSHAHIARIRDLTLPLLGAKLA
CCCCHHHHCCHHHHHHHEEECHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCC
PFEAFLLTRGLRTLKVRMMQHQATADLFVDRLAAVPQVRRINSPGANAVPGLTGRSGLMS
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEE
IEFDDTVDIPAFADAFTHFRLGVSWGGFESLVLPARVALAQAGEHNSLQAFGVSPNLVRL
EECCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEE
NLGLEDPEDLWADFESALSASLT
EECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]