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The map label for this gene is appF [H]

Identifier: 89055784

GI number: 89055784

Start: 3347023

End: 3348279

Strand: Direct

Name: appF [H]

Synonym: Jann_3293

Alternate gene names: 89055784

Gene position: 3347023-3348279 (Clockwise)

Preceding gene: 89055783

Following gene: 89055785

Centisome position: 77.51

GC content: 62.21

Gene sequence:

>1257_bases
ATGCTTGACGATACCTCCGCGGCGGCGCGCAAGCTGGTCGAGGTGCGCAACCTGCGCATGTATTTCCCGATCCGCACCGG
GCTGCTGCGCCGGCACACCGGCGATGTGAAAGCCGTCGATGGGGTGAACTTCGATATCTATGAGGGCGAAACCCTTGGTC
TTGTGGGCGAAAGCGGGTGTGGAAAGTCCACCTGTGGCCGCGCGGTTATCCGGCTCTATGAGCCCACCGATGGCTCCATC
CAGCTGGAAAACAAGGAGCTTGCCTGGTCCAACATCAAGGCCCGCGACATTGCGCAGCGGGCCGAACGCGTCGCCTCGGT
GGTCTTCTTCGTGGCCTTCGCCCTGACCCTTGTGGGCTTGTTCTTTGCGTTTCCTGCAATTGCCCTGGGTTTTGCAACCT
ACCCGATCCTGACCGTGGTGGCGCTGACGCTGTTTTTGGCGGCGACCTCGCTCTACTATTACATCCCGAAATGGATCTAC
CGCTCCGTCGTTGCGACGCGGGAGGGGGTGTCCACCCGGGATTTTGCTCCGTCCGAGCGGGAGTTGCGGCGCATCCGGCC
TACGATGCAGATGGTCTTCCAGGACCCACAGGCCTCGCTCAACCCGCGCATGACCGTGGCCGCCATCATCGGCGAGCCGC
TGTCTGAGCATACGACCCTGTCCAAGGCCGAGCGCAATGATCGCATCTATGAGCTTCTGGACGCCGTCGGCCTCAACCGT
GATTTCGCCAACCGCTATCCGCACGAATTTTCCGGTGGGCAGCGCCAGCGGATCGGCATCGCACGGGCGCTTGCCCTCAA
TCCCAAGTTCATTGTCTGTGACGAGCCGATTGCCGCATTGGACGTGTCGATCCAGGCGCAGGTGGTAAACCTGCTGGAGG
ATTTGCAGGAAAAGCTGGGGCTGACCTATCTGTTCATCTCCCATGACCTGTCGATGGTGCGCCATATCGCGGACCGTGTG
GCGGTGATGTATCTGGGCAAAGTGGCCGAGCTGGCCCCGCGCGACGCGCTGTTCAGCGATCCGCTGCACCCCTACACCAA
GGCGCTTCTGTCCGCCGTGCCCGAGCCCGATCCGGCGTCCCACGACGTGGCGGAGCGGGTGATTTTGCAAGGCGATGTCC
CGTCGCCGTCCAACCCACCCAAGGGTTGCAACTTCTGCACACGCTGCCCCGCAGTGATGGACGTGTGCCGCATTGAAGAT
CCCGAAATGAAAGAGGTGCGCCCGGGCCGTTTCGTCGCCTGCCACCTGCACAGTTAA

Upstream 100 bases:

>100_bases
CCCCATGCATTTGATCGGTGCGCGCGCGAAAACCCTCAGCTGGCCTCCGTCGGTGAGAATGACCACAACGCGGCCTGTTT
CCTGGACGGAGGATCGACAG

Downstream 100 bases:

>100_bases
AAGCAACAAGATCGCCCCAAGGCGGCACGAGCAAACTGACAAAGACCGACCACGACCAACAAGGAGTGAGAGACACAGTA
ATGAAACGTACATCCATTCT

Product: oligopeptide/dipeptide ABC transporter ATP-binding protein-like protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLDDTSAAARKLVEVRNLRMYFPIRTGLLRRHTGDVKAVDGVNFDIYEGETLGLVGESGCGKSTCGRAVIRLYEPTDGSI
QLENKELAWSNIKARDIAQRAERVASVVFFVAFALTLVGLFFAFPAIALGFATYPILTVVALTLFLAATSLYYYIPKWIY
RSVVATREGVSTRDFAPSERELRRIRPTMQMVFQDPQASLNPRMTVAAIIGEPLSEHTTLSKAERNDRIYELLDAVGLNR
DFANRYPHEFSGGQRQRIGIARALALNPKFIVCDEPIAALDVSIQAQVVNLLEDLQEKLGLTYLFISHDLSMVRHIADRV
AVMYLGKVAELAPRDALFSDPLHPYTKALLSAVPEPDPASHDVAERVILQGDVPSPSNPPKGCNFCTRCPAVMDVCRIED
PEMKEVRPGRFVACHLHS

Sequences:

>Translated_418_residues
MLDDTSAAARKLVEVRNLRMYFPIRTGLLRRHTGDVKAVDGVNFDIYEGETLGLVGESGCGKSTCGRAVIRLYEPTDGSI
QLENKELAWSNIKARDIAQRAERVASVVFFVAFALTLVGLFFAFPAIALGFATYPILTVVALTLFLAATSLYYYIPKWIY
RSVVATREGVSTRDFAPSERELRRIRPTMQMVFQDPQASLNPRMTVAAIIGEPLSEHTTLSKAERNDRIYELLDAVGLNR
DFANRYPHEFSGGQRQRIGIARALALNPKFIVCDEPIAALDVSIQAQVVNLLEDLQEKLGLTYLFISHDLSMVRHIADRV
AVMYLGKVAELAPRDALFSDPLHPYTKALLSAVPEPDPASHDVAERVILQGDVPSPSNPPKGCNFCTRCPAVMDVCRIED
PEMKEVRPGRFVACHLHS
>Mature_418_residues
MLDDTSAAARKLVEVRNLRMYFPIRTGLLRRHTGDVKAVDGVNFDIYEGETLGLVGESGCGKSTCGRAVIRLYEPTDGSI
QLENKELAWSNIKARDIAQRAERVASVVFFVAFALTLVGLFFAFPAIALGFATYPILTVVALTLFLAATSLYYYIPKWIY
RSVVATREGVSTRDFAPSERELRRIRPTMQMVFQDPQASLNPRMTVAAIIGEPLSEHTTLSKAERNDRIYELLDAVGLNR
DFANRYPHEFSGGQRQRIGIARALALNPKFIVCDEPIAALDVSIQAQVVNLLEDLQEKLGLTYLFISHDLSMVRHIADRV
AVMYLGKVAELAPRDALFSDPLHPYTKALLSAVPEPDPASHDVAERVILQGDVPSPSNPPKGCNFCTRCPAVMDVCRIED
PEMKEVRPGRFVACHLHS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG4608

COG function: function code E; ABC-type oligopeptide transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI171184400, Length=134, Percent_Identity=37.3134328358209, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787500, Length=229, Percent_Identity=56.3318777292576, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1789962, Length=238, Percent_Identity=51.2605042016807, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI145693107, Length=233, Percent_Identity=49.7854077253219, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787758, Length=211, Percent_Identity=49.2890995260664, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789963, Length=242, Percent_Identity=42.5619834710744, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI48994883, Length=239, Percent_Identity=41.8410041841004, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788506, Length=175, Percent_Identity=48.5714285714286, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787547, Length=164, Percent_Identity=42.0731707317073, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787759, Length=223, Percent_Identity=36.322869955157, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787548, Length=247, Percent_Identity=33.1983805668016, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789891, Length=172, Percent_Identity=40.1162790697674, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1786398, Length=180, Percent_Identity=38.8888888888889, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI48994997, Length=180, Percent_Identity=36.1111111111111, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789032, Length=177, Percent_Identity=37.8531073446328, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788644, Length=174, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786872, Length=192, Percent_Identity=32.2916666666667, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081791, Length=169, Percent_Identity=34.9112426035503, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788225, Length=189, Percent_Identity=36.5079365079365, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787089, Length=180, Percent_Identity=36.1111111111111, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787370, Length=162, Percent_Identity=34.5679012345679, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789890, Length=173, Percent_Identity=35.2601156069364, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789672, Length=174, Percent_Identity=33.9080459770115, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1787712, Length=184, Percent_Identity=33.1521739130435, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788761, Length=130, Percent_Identity=36.1538461538462, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787029, Length=160, Percent_Identity=36.875, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1788450, Length=167, Percent_Identity=31.1377245508982, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87081709, Length=161, Percent_Identity=31.055900621118, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790467, Length=196, Percent_Identity=27.0408163265306, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081834, Length=110, Percent_Identity=39.0909090909091, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790162, Length=178, Percent_Identity=26.9662921348315, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786563, Length=107, Percent_Identity=38.3177570093458, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787164, Length=148, Percent_Identity=35.1351351351351, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87082267, Length=212, Percent_Identity=26.8867924528302, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789586, Length=180, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786253, Length=174, Percent_Identity=29.3103448275862, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789873, Length=139, Percent_Identity=32.3741007194245, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786345, Length=147, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787105, Length=93, Percent_Identity=38.7096774193548, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789593, Length=132, Percent_Identity=31.0606060606061, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI48995001, Length=141, Percent_Identity=34.0425531914894, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790544, Length=156, Percent_Identity=32.0512820512821, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=161, Percent_Identity=32.2981366459627, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558664, Length=154, Percent_Identity=34.4155844155844, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=136, Percent_Identity=33.8235294117647, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193209708, Length=93, Percent_Identity=40.8602150537634, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324985, Length=140, Percent_Identity=31.4285714285714, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136662, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641565, Length=90, Percent_Identity=42.2222222222222, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363262, Length=118, Percent_Identity=35.5932203389831, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653245, Length=118, Percent_Identity=35.5932203389831, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 46434; Mature: 46434

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDTSAAARKLVEVRNLRMYFPIRTGLLRRHTGDVKAVDGVNFDIYEGETLGLVGESGC
CCCCHHHHHHHHHHHHHHHEEECHHHHHHHHCCCCEEEECCCCEEEECCCEEEEECCCCC
GKSTCGRAVIRLYEPTDGSIQLENKELAWSNIKARDIAQRAERVASVVFFVAFALTLVGL
CHHHHHHHEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFAFPAIALGFATYPILTVVALTLFLAATSLYYYIPKWIYRSVVATREGVSTRDFAPSER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ELRRIRPTMQMVFQDPQASLNPRMTVAAIIGEPLSEHTTLSKAERNDRIYELLDAVGLNR
HHHHHHHHHHHHHCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFANRYPHEFSGGQRQRIGIARALALNPKFIVCDEPIAALDVSIQAQVVNLLEDLQEKLG
HHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
LTYLFISHDLSMVRHIADRVAVMYLGKVAELAPRDALFSDPLHPYTKALLSAVPEPDPAS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCCC
HDVAERVILQGDVPSPSNPPKGCNFCTRCPAVMDVCRIEDPEMKEVRPGRFVACHLHS
HHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCCCHHHCCCCCEEEEEECC
>Mature Secondary Structure
MLDDTSAAARKLVEVRNLRMYFPIRTGLLRRHTGDVKAVDGVNFDIYEGETLGLVGESGC
CCCCHHHHHHHHHHHHHHHEEECHHHHHHHHCCCCEEEECCCCEEEECCCEEEEECCCCC
GKSTCGRAVIRLYEPTDGSIQLENKELAWSNIKARDIAQRAERVASVVFFVAFALTLVGL
CHHHHHHHEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFAFPAIALGFATYPILTVVALTLFLAATSLYYYIPKWIYRSVVATREGVSTRDFAPSER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ELRRIRPTMQMVFQDPQASLNPRMTVAAIIGEPLSEHTTLSKAERNDRIYELLDAVGLNR
HHHHHHHHHHHHHCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFANRYPHEFSGGQRQRIGIARALALNPKFIVCDEPIAALDVSIQAQVVNLLEDLQEKLG
HHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
LTYLFISHDLSMVRHIADRVAVMYLGKVAELAPRDALFSDPLHPYTKALLSAVPEPDPAS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCCCC
HDVAERVILQGDVPSPSNPPKGCNFCTRCPAVMDVCRIEDPEMKEVRPGRFVACHLHS
HHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCCCHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]