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The map label for this gene is ynfL [H]

Identifier: 89055740

GI number: 89055740

Start: 3298990

End: 3299883

Strand: Direct

Name: ynfL [H]

Synonym: Jann_3249

Alternate gene names: 89055740

Gene position: 3298990-3299883 (Clockwise)

Preceding gene: 89055733

Following gene: 89055741

Centisome position: 76.4

GC content: 64.65

Gene sequence:

>894_bases
ATGCGTTCTGATCTGCGTCAGATCCGCTATTTCGTGGCCACGGCGGAGGAGCTTCACTTTCGCCGGGCCGCTGACCGTCT
GGGCATCGCGCAACCGGCGCTGAGCCGCGCGATCCAGGCGTTGGAGGCCGAGTTGGACGTGGCGCTGTTTGTGCGTACGA
ACCGCTCCGTCCAGATCACCACGGCGGGTCGCGTGTTTCTCGACGGCGCGCGGGAAATTCTGACCCTGACCGAGCGTATG
TTGGAAGAAACCCGCACCGCCCATGACGGGCGCATGGGCGCGTTGCGCATCGGCTATACCGACTTCGCCATCGCCGGAGA
GCTGCCGAGCCTGCTGAAGCACTTTCAGGAACGCCACCCGCGCGTGACCCTGAAGCCGCAGCACGGCGTCACGCAGACCC
AAATCCGGCTGTTGGAGGAGGGCAAGCTGGATCTGGGCTTTGTCACCGGTCCCATCGTGCGCGACGGCTTTGACCAGCGG
GTCGTCCAATCCGAAGCACTGCAAGCCGTTGTCCCCGACAGCCACCCCTTTGCCGGGCGCGCAACCCTGTCCATCGCGGA
CCTGGCGGAGGAGGATTTTGTCCACGGCAGTTCCAGGGATTGGCAACATTTCTACGACTATCTCTTGCCGATGTGTCGCC
GTGCCGGATTCTCGCCCCGGATCGTGCAGGAGGCCTTCAACACCGCCGGTATCCTTGGCCTCGTGTCCTGTGGGATGGGC
GTCACGGTGCTGACCGAACGGGTCTGTTCGGCCCTTGGTCCGGGGTTGGTCGCGATCCCGATCACGGACGCGGACGAACG
CCTGGTGACTTCCGCCATTTGGCGCAAGGACCACCTGTCTGGGCCCGCGCGACACTTCGTAGAGGATCTCTGTGCGCGGC
CGCCGCTTCCCTAA

Upstream 100 bases:

>100_bases
TGGATTCCTCCTTGTTGGTGATTGTTTTTCTTTCAGCTTTGCATAGGAAAAGCAGTGCGTATAATATCAATAATGGCATT
TTTGATACTCTATCGGCATC

Downstream 100 bases:

>100_bases
AGCGCTTGCCTTTGGCTGCATCTGCGATACCCCTTGGAGCGCGCGTCAGGAGGGCGGACATGTCAGAGATCACTGTTTTC
AAAGCGAAAACCATCGTCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRSDLRQIRYFVATAEELHFRRAADRLGIAQPALSRAIQALEAELDVALFVRTNRSVQITTAGRVFLDGAREILTLTERM
LEETRTAHDGRMGALRIGYTDFAIAGELPSLLKHFQERHPRVTLKPQHGVTQTQIRLLEEGKLDLGFVTGPIVRDGFDQR
VVQSEALQAVVPDSHPFAGRATLSIADLAEEDFVHGSSRDWQHFYDYLLPMCRRAGFSPRIVQEAFNTAGILGLVSCGMG
VTVLTERVCSALGPGLVAIPITDADERLVTSAIWRKDHLSGPARHFVEDLCARPPLP

Sequences:

>Translated_297_residues
MRSDLRQIRYFVATAEELHFRRAADRLGIAQPALSRAIQALEAELDVALFVRTNRSVQITTAGRVFLDGAREILTLTERM
LEETRTAHDGRMGALRIGYTDFAIAGELPSLLKHFQERHPRVTLKPQHGVTQTQIRLLEEGKLDLGFVTGPIVRDGFDQR
VVQSEALQAVVPDSHPFAGRATLSIADLAEEDFVHGSSRDWQHFYDYLLPMCRRAGFSPRIVQEAFNTAGILGLVSCGMG
VTVLTERVCSALGPGLVAIPITDADERLVTSAIWRKDHLSGPARHFVEDLCARPPLP
>Mature_297_residues
MRSDLRQIRYFVATAEELHFRRAADRLGIAQPALSRAIQALEAELDVALFVRTNRSVQITTAGRVFLDGAREILTLTERM
LEETRTAHDGRMGALRIGYTDFAIAGELPSLLKHFQERHPRVTLKPQHGVTQTQIRLLEEGKLDLGFVTGPIVRDGFDQR
VVQSEALQAVVPDSHPFAGRATLSIADLAEEDFVHGSSRDWQHFYDYLLPMCRRAGFSPRIVQEAFNTAGILGLVSCGMG
VTVLTERVCSALGPGLVAIPITDADERLVTSAIWRKDHLSGPARHFVEDLCARPPLP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=37.8472222222222, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=31.6981132075472, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=31.0344827586207, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790399, Length=277, Percent_Identity=28.158844765343, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI157672245, Length=238, Percent_Identity=31.5126050420168, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=27.0547945205479, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=298, Percent_Identity=24.1610738255034, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787601, Length=290, Percent_Identity=27.2413793103448, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787530, Length=301, Percent_Identity=28.2392026578073, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788748, Length=256, Percent_Identity=26.171875, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=235, Percent_Identity=27.2340425531915, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=160, Percent_Identity=31.25, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=26.2948207171315, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786713, Length=182, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=309, Percent_Identity=25.8899676375405, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787128, Length=125, Percent_Identity=32.8, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=248, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32942; Mature: 32942

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDLRQIRYFVATAEELHFRRAADRLGIAQPALSRAIQALEAELDVALFVRTNRSVQIT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEE
TAGRVFLDGAREILTLTERMLEETRTAHDGRMGALRIGYTDFAIAGELPSLLKHFQERHP
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
RVTLKPQHGVTQTQIRLLEEGKLDLGFVTGPIVRDGFDQRVVQSEALQAVVPDSHPFAGR
EEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
ATLSIADLAEEDFVHGSSRDWQHFYDYLLPMCRRAGFSPRIVQEAFNTAGILGLVSCGMG
EEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
VTVLTERVCSALGPGLVAIPITDADERLVTSAIWRKDHLSGPARHFVEDLCARPPLP
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRSDLRQIRYFVATAEELHFRRAADRLGIAQPALSRAIQALEAELDVALFVRTNRSVQIT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEE
TAGRVFLDGAREILTLTERMLEETRTAHDGRMGALRIGYTDFAIAGELPSLLKHFQERHP
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
RVTLKPQHGVTQTQIRLLEEGKLDLGFVTGPIVRDGFDQRVVQSEALQAVVPDSHPFAGR
EEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
ATLSIADLAEEDFVHGSSRDWQHFYDYLLPMCRRAGFSPRIVQEAFNTAGILGLVSCGMG
EEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
VTVLTERVCSALGPGLVAIPITDADERLVTSAIWRKDHLSGPARHFVEDLCARPPLP
HHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]