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The map label for this gene is potD-A [H]

Identifier: 89055725

GI number: 89055725

Start: 3282153

End: 3283190

Strand: Direct

Name: potD-A [H]

Synonym: Jann_3234

Alternate gene names: 89055725

Gene position: 3282153-3283190 (Clockwise)

Preceding gene: 89055724

Following gene: 89055726

Centisome position: 76.01

GC content: 60.79

Gene sequence:

>1038_bases
ATGACACATCGACTGACTTTGAGCCTTGCGACCGTTTCCGCCGCTGCCATTCTGGCCGCCACGCCCGCCTTGGCGCAGTC
CCGCACCTTCACCGTTTCCTGGTGGGGGTTCAACGGCGACGCGTTGCAGGAAATCATTATCGATCCGTTCCAGGAGATGT
GCGATTGCGAAGTCATCTTCGAGACGGGCAACAACTCCGACCGCCTCAGCCGCCTGCAATTGCGGGAGGGAGCGGGCGTG
GACGTGATCTACCTGACAGATCGCTTCTCACAATTGGGGATCGAGTTGGGTCTGTTCCAACCAATCGACCGCGCCCAGGT
GCCCAATATCGACGAAATCTATGACCTGGCCCAAGCGCCACAGGGTGAATACGGCCCCGCCTATTCCATCGGGCGCGTTG
GTATCGTCTACAATGCCGACGAAGTCGAAGCACCGATCACGTCCTGGGATGATCTGTGGCGCGAAGATCTGGCAGGCTCC
GTCTCACTGCCCGGCATCACGACGACGGCAGGGCCGATGGTCGTCCTTCGTGCCGGCGCGCGCGCGGGTGTCGACGCTTA
CGAGGACCCGGACGCCGCCTTTGCGGAAGTGGCCGAGATCGTGCCCAACGTGCTGACCAACTACAACACCGGATCCGAGA
TGATTAACCTCTTCTCCACCGGGGAAATCACCGTTTCCATGGCGCAGGATTTCGCGTTGGGGCGTTTGCAGGCCGCCGTC
CCGTCGGTCGTGTGGGCGGATCTGGAGGATGGCGCGATTGCCACGCTTAACACGATAAACATCCCCACCGGGTCTGAGAA
TGTGGAGCTGGCGCACCAGTTCCTCAACTTCGTGCTGTCCGCTGAAATCCAGCAAGAGCTGGCCGAGCGCGGCGTCGATG
CGCCGGTCAACAACGGCGTTGATCTGACCGAGGAGCAGGCCGCGCCCTGGACCTATGGCGATGAGATGATCGCAGGTCTG
AACCGCATGGACTACGTGCCGATGAACGCCAATCTCGACGACTGGATCAATCGCTGGAACGAAGTCTTCGGCATGTAA

Upstream 100 bases:

>100_bases
CCGGTAGAACTTGCACCTCCGCAAATTGCTCCATACGCTTGAGTTATTACAAGGGGTGCTCGCGTCGTCTGTAGAAGTGC
CCGGAGAACAGGGGAAATCC

Downstream 100 bases:

>100_bases
ACGCGTGTTGGCCAGGACATTCCGGGGCGGATCCCTGCCCCGGAGATCTGGCCTCTGCCCAACGGAAGCGCCCCAATGAA
ACGCCTTGCAGGCTGGATGT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTHRLTLSLATVSAAAILAATPALAQSRTFTVSWWGFNGDALQEIIIDPFQEMCDCEVIFETGNNSDRLSRLQLREGAGV
DVIYLTDRFSQLGIELGLFQPIDRAQVPNIDEIYDLAQAPQGEYGPAYSIGRVGIVYNADEVEAPITSWDDLWREDLAGS
VSLPGITTTAGPMVVLRAGARAGVDAYEDPDAAFAEVAEIVPNVLTNYNTGSEMINLFSTGEITVSMAQDFALGRLQAAV
PSVVWADLEDGAIATLNTINIPTGSENVELAHQFLNFVLSAEIQQELAERGVDAPVNNGVDLTEEQAAPWTYGDEMIAGL
NRMDYVPMNANLDDWINRWNEVFGM

Sequences:

>Translated_345_residues
MTHRLTLSLATVSAAAILAATPALAQSRTFTVSWWGFNGDALQEIIIDPFQEMCDCEVIFETGNNSDRLSRLQLREGAGV
DVIYLTDRFSQLGIELGLFQPIDRAQVPNIDEIYDLAQAPQGEYGPAYSIGRVGIVYNADEVEAPITSWDDLWREDLAGS
VSLPGITTTAGPMVVLRAGARAGVDAYEDPDAAFAEVAEIVPNVLTNYNTGSEMINLFSTGEITVSMAQDFALGRLQAAV
PSVVWADLEDGAIATLNTINIPTGSENVELAHQFLNFVLSAEIQQELAERGVDAPVNNGVDLTEEQAAPWTYGDEMIAGL
NRMDYVPMNANLDDWINRWNEVFGM
>Mature_344_residues
THRLTLSLATVSAAAILAATPALAQSRTFTVSWWGFNGDALQEIIIDPFQEMCDCEVIFETGNNSDRLSRLQLREGAGVD
VIYLTDRFSQLGIELGLFQPIDRAQVPNIDEIYDLAQAPQGEYGPAYSIGRVGIVYNADEVEAPITSWDDLWREDLAGSV
SLPGITTTAGPMVVLRAGARAGVDAYEDPDAAFAEVAEIVPNVLTNYNTGSEMINLFSTGEITVSMAQDFALGRLQAAVP
SVVWADLEDGAIATLNTINIPTGSENVELAHQFLNFVLSAEIQQELAERGVDAPVNNGVDLTEEQAAPWTYGDEMIAGLN
RMDYVPMNANLDDWINRWNEVFGM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=252, Percent_Identity=24.2063492063492, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787078, Length=313, Percent_Identity=23.0031948881789, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37527; Mature: 37396

Theoretical pI: Translated: 3.71; Mature: 3.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRLTLSLATVSAAAILAATPALAQSRTFTVSWWGFNGDALQEIIIDPFQEMCDCEVIF
CCEEEEEEHHHHHHHHHHHHCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
ETGNNSDRLSRLQLREGAGVDVIYLTDRFSQLGIELGLFQPIDRAQVPNIDEIYDLAQAP
ECCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCCEECCCCCCCCCCCCCHHHHHHHHHCC
QGEYGPAYSIGRVGIVYNADEVEAPITSWDDLWREDLAGSVSLPGITTTAGPMVVLRAGA
CCCCCCCCCCCCEEEEECCHHCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCEEEEECCC
RAGVDAYEDPDAAFAEVAEIVPNVLTNYNTGSEMINLFSTGEITVSMAQDFALGRLQAAV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCEEEEEHHHHHHHHHHHHH
PSVVWADLEDGAIATLNTINIPTGSENVELAHQFLNFVLSAEIQQELAERGVDAPVNNGV
HHHEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DLTEEQAAPWTYGDEMIAGLNRMDYVPMNANLDDWINRWNEVFGM
CCCCCCCCCCCCCHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
THRLTLSLATVSAAAILAATPALAQSRTFTVSWWGFNGDALQEIIIDPFQEMCDCEVIF
CEEEEEEHHHHHHHHHHHHCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
ETGNNSDRLSRLQLREGAGVDVIYLTDRFSQLGIELGLFQPIDRAQVPNIDEIYDLAQAP
ECCCCHHHHHHHHHHCCCCEEEEEEECCHHHCCCEECCCCCCCCCCCCCHHHHHHHHHCC
QGEYGPAYSIGRVGIVYNADEVEAPITSWDDLWREDLAGSVSLPGITTTAGPMVVLRAGA
CCCCCCCCCCCCEEEEECCHHCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCEEEEECCC
RAGVDAYEDPDAAFAEVAEIVPNVLTNYNTGSEMINLFSTGEITVSMAQDFALGRLQAAV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCEEEEEHHHHHHHHHHHHH
PSVVWADLEDGAIATLNTINIPTGSENVELAHQFLNFVLSAEIQQELAERGVDAPVNNGV
HHHEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DLTEEQAAPWTYGDEMIAGLNRMDYVPMNANLDDWINRWNEVFGM
CCCCCCCCCCCCCHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]