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The map label for this gene is glgX [H]

Identifier: 89055607

GI number: 89055607

Start: 3152393

End: 3154489

Strand: Direct

Name: glgX [H]

Synonym: Jann_3116

Alternate gene names: 89055607

Gene position: 3152393-3154489 (Clockwise)

Preceding gene: 89055606

Following gene: 89055608

Centisome position: 73.01

GC content: 61.56

Gene sequence:

>2097_bases
ATGCTTGAAAACTTTCACGTAGAGGCGGGCGCACCCAATCCTCTGGGCGCGACCTTCGATGGCGGCGGCGTGAACTTCGC
CGTCTTCTCCAGGCACGCCACACAGGTGATGCTATGCCTGTTTAATGAGGCCGGAGAAGAGACGCATCTGATCGCCATGC
CCGAGCGAGACGGCCATGTCTGGCACGGCTATGTCCCCGGAATGGGACCGGGCCAGCAATACGGCTACCGCGTTCACGGA
CCCTATGCGCCCGAAGATGGGCACCGCTTCAACCCCTATAAGCTGGTGCTCGACCCCTACGCAAAACGCCTGACCGGGCA
GCCGATCTGGAACGATGCGCTTTACGGCTATGTCCATGGCAAAAGCGATCTGACGTTCGACACACGCGACAGCGCGCCCT
ACATGCCGCGCTGCATTGTCACCGATCCCGCCTTCAGCTGGGGGGCGGATGCGGCCCCGCGTGTTCCGATGGACGAGACC
GTATTCTACGAGGCCCATGTGAAAGGCCTTACCGCGACCCATCCGGGGGTTGAGGCACCCGGCACCTACCTTGGCATGGC
GTCCCCGCCCATCCTCGACCACCTTACCGATTTGGGCGTGACCACGGTGGAGCTGTTGCCCGTCCAGGCCTTCGTCGACG
ACCGCTTCCTGGTCGACAAGGGTCTGCGCAACTACTGGGGCTACATGACCTATGGCTTCTTTGCGCCAGAGCCTCGGTAC
ATGTCCAACGGGTCCATCGCGGAGTTCCAACAGATGGTGGCGCGCTACCATTCGGCCGGGATCGAGGTCATTCTGGACGT
CGTCTACAATCACACGGCGGAGGGGTCCGAATTTGGTCCGACACTCGCATTCCGGGGCCTCGACAACGCCAGCTACTATC
GGTTGACCGATGACAAACGCTACTACGTCAACGATGCGGGCTGTGGAAACTCATTGAATTTTGATAATCCCTTCACCCTG
CGCATGGTCATGGATTCCCTGCGGTATTGGGTTGAGGTCATGCATATCGACGGCTTCCGGTTCGATTTGTGCACATCCCT
TGGCCGCACAGATGGCGTCTTTGACCGCGACGGACCCTTCTTCCGGGCGATCCGGCAAGACCCTGTCCTGAACCGTGTGA
AACTGATTGCGGAGCCGTGGGATCTGGGCCCTGACGGCTACCAACTGGGGGCCTATCAGGCCCCCTTCGGCGAATGGAAT
GACAAATTTCGCGACGACACGCGGCGGTTCTGGCGCGGAGACGCAGGCATGGTGGCGGCGATGGCCGGGCGGCTTTCGGG
CTCGGCCATGCATTTCGACCACGATGGGCGCGCAGCCACGGCCTCGGTCAATTTCATCACGGCCCACGACGGCTTCACCC
TGTCGGATCTGGTCAGCTACCGCGAGAAACACAACGAGGCCAATGGAGAGGATAATCGCGACGGGCATTCCAACAACCAT
TCCGACAATTTCGGGGTGGAGGGTGAGACGGATGACCCCGATATCCTCGCCGCCCGTACTCAGCGCCGCCGCAACCTGAT
CGCCACGCTGATGTTGTCGCAAGGCACGCCGCTGATGCTGGCGGGCGATGAAATCGGCAATACCCAGGGCGGCAACAACA
ACACCTATTGTCAGGATAGCGAGATCGGCTGGGTCGATTGGGCGGGTCGCGACGATGCCTTCTATGCCTTCTGCAAGGCC
GCGATTGCGTTTCGCAAAGCTCACCCGATCCTGCGACAGCGGCGGTTCCTGCACTCCAAGCCGCGCTTCATTGACGGCGC
GCCGGATCTGTACTGGCGCAGGACCGATGGCACGCCGATGCGCCAGACCGATTGGGACAATCCGGACCTGAAGATCATCG
TGGCCGAGATGCGCATGGCCTCGGGCACACCCGCCTACATCAAGCGCGAAGGCGCGCTGCTGGTCGTCCTGAATGTGGGC
GGCGCCGCTGACATTACCCTGCCTACACTGGATACCGGCCAATGGGTCCGCGTTTTCGACAGCGCACAACCCGACCCAAC
CGCCCTGCCCCCCGGCGCAGACCCGCAGATCGACGCCAATTGCGTCGTCGTCTATGAGCTCCAACCCAACACGGAAGGAC
ATAATCATGCAGCCTGA

Upstream 100 bases:

>100_bases
GATGGTGCGCAACGCCATGAAGCAGCCGGTGGGGTGGGACATGTCTGCCTCCCGCTACCATGCGCTTTATAAGGCCACCG
CCCGCAAATGATCGATCGCT

Downstream 100 bases:

>100_bases
TACCGTTGCCACGCAGCCCATCGCGGGCCAGAAGCCCGGCACATCCGGCCTTCGCAAGAAAACCCGCACCTTCATGGAGC
CGCACTTTCTGGAAAACTAC

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MLENFHVEAGAPNPLGATFDGGGVNFAVFSRHATQVMLCLFNEAGEETHLIAMPERDGHVWHGYVPGMGPGQQYGYRVHG
PYAPEDGHRFNPYKLVLDPYAKRLTGQPIWNDALYGYVHGKSDLTFDTRDSAPYMPRCIVTDPAFSWGADAAPRVPMDET
VFYEAHVKGLTATHPGVEAPGTYLGMASPPILDHLTDLGVTTVELLPVQAFVDDRFLVDKGLRNYWGYMTYGFFAPEPRY
MSNGSIAEFQQMVARYHSAGIEVILDVVYNHTAEGSEFGPTLAFRGLDNASYYRLTDDKRYYVNDAGCGNSLNFDNPFTL
RMVMDSLRYWVEVMHIDGFRFDLCTSLGRTDGVFDRDGPFFRAIRQDPVLNRVKLIAEPWDLGPDGYQLGAYQAPFGEWN
DKFRDDTRRFWRGDAGMVAAMAGRLSGSAMHFDHDGRAATASVNFITAHDGFTLSDLVSYREKHNEANGEDNRDGHSNNH
SDNFGVEGETDDPDILAARTQRRRNLIATLMLSQGTPLMLAGDEIGNTQGGNNNTYCQDSEIGWVDWAGRDDAFYAFCKA
AIAFRKAHPILRQRRFLHSKPRFIDGAPDLYWRRTDGTPMRQTDWDNPDLKIIVAEMRMASGTPAYIKREGALLVVLNVG
GAADITLPTLDTGQWVRVFDSAQPDPTALPPGADPQIDANCVVVYELQPNTEGHNHAA

Sequences:

>Translated_698_residues
MLENFHVEAGAPNPLGATFDGGGVNFAVFSRHATQVMLCLFNEAGEETHLIAMPERDGHVWHGYVPGMGPGQQYGYRVHG
PYAPEDGHRFNPYKLVLDPYAKRLTGQPIWNDALYGYVHGKSDLTFDTRDSAPYMPRCIVTDPAFSWGADAAPRVPMDET
VFYEAHVKGLTATHPGVEAPGTYLGMASPPILDHLTDLGVTTVELLPVQAFVDDRFLVDKGLRNYWGYMTYGFFAPEPRY
MSNGSIAEFQQMVARYHSAGIEVILDVVYNHTAEGSEFGPTLAFRGLDNASYYRLTDDKRYYVNDAGCGNSLNFDNPFTL
RMVMDSLRYWVEVMHIDGFRFDLCTSLGRTDGVFDRDGPFFRAIRQDPVLNRVKLIAEPWDLGPDGYQLGAYQAPFGEWN
DKFRDDTRRFWRGDAGMVAAMAGRLSGSAMHFDHDGRAATASVNFITAHDGFTLSDLVSYREKHNEANGEDNRDGHSNNH
SDNFGVEGETDDPDILAARTQRRRNLIATLMLSQGTPLMLAGDEIGNTQGGNNNTYCQDSEIGWVDWAGRDDAFYAFCKA
AIAFRKAHPILRQRRFLHSKPRFIDGAPDLYWRRTDGTPMRQTDWDNPDLKIIVAEMRMASGTPAYIKREGALLVVLNVG
GAADITLPTLDTGQWVRVFDSAQPDPTALPPGADPQIDANCVVVYELQPNTEGHNHAA
>Mature_698_residues
MLENFHVEAGAPNPLGATFDGGGVNFAVFSRHATQVMLCLFNEAGEETHLIAMPERDGHVWHGYVPGMGPGQQYGYRVHG
PYAPEDGHRFNPYKLVLDPYAKRLTGQPIWNDALYGYVHGKSDLTFDTRDSAPYMPRCIVTDPAFSWGADAAPRVPMDET
VFYEAHVKGLTATHPGVEAPGTYLGMASPPILDHLTDLGVTTVELLPVQAFVDDRFLVDKGLRNYWGYMTYGFFAPEPRY
MSNGSIAEFQQMVARYHSAGIEVILDVVYNHTAEGSEFGPTLAFRGLDNASYYRLTDDKRYYVNDAGCGNSLNFDNPFTL
RMVMDSLRYWVEVMHIDGFRFDLCTSLGRTDGVFDRDGPFFRAIRQDPVLNRVKLIAEPWDLGPDGYQLGAYQAPFGEWN
DKFRDDTRRFWRGDAGMVAAMAGRLSGSAMHFDHDGRAATASVNFITAHDGFTLSDLVSYREKHNEANGEDNRDGHSNNH
SDNFGVEGETDDPDILAARTQRRRNLIATLMLSQGTPLMLAGDEIGNTQGGNNNTYCQDSEIGWVDWAGRDDAFYAFCKA
AIAFRKAHPILRQRRFLHSKPRFIDGAPDLYWRRTDGTPMRQTDWDNPDLKIIVAEMRMASGTPAYIKREGALLVVLNVG
GAADITLPTLDTGQWVRVFDSAQPDPTALPPGADPQIDANCVVVYELQPNTEGHNHAA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=251, Percent_Identity=24.3027888446215, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI2367229, Length=613, Percent_Identity=45.8401305057096, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6320826, Length=200, Percent_Identity=26.5, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77731; Mature: 77731

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENFHVEAGAPNPLGATFDGGGVNFAVFSRHATQVMLCLFNEAGEETHLIAMPERDGHV
CCCCEEEECCCCCCCCCEECCCCEEEEEECCCHHHEEHHHHHCCCCCEEEEEECCCCCCE
WHGYVPGMGPGQQYGYRVHGPYAPEDGHRFNPYKLVLDPYAKRLTGQPIWNDALYGYVHG
EEEECCCCCCCHHCCEEECCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
KSDLTFDTRDSAPYMPRCIVTDPAFSWGADAAPRVPMDETVFYEAHVKGLTATHPGVEAP
CCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCCCCCC
GTYLGMASPPILDHLTDLGVTTVELLPVQAFVDDRFLVDKGLRNYWGYMTYGFFAPEPRY
CCEECCCCCHHHHHHHHCCCEEEEEEEEHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCC
MSNGSIAEFQQMVARYHSAGIEVILDVVYNHTAEGSEFGPTLAFRGLDNASYYRLTDDKR
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCE
YYVNDAGCGNSLNFDNPFTLRMVMDSLRYWVEVMHIDGFRFDLCTSLGRTDGVFDRDGPF
EEEECCCCCCCCCCCCCEEHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCCCCCCHH
FRAIRQDPVLNRVKLIAEPWDLGPDGYQLGAYQAPFGEWNDKFRDDTRRFWRGDAGMVAA
HHHHHCCCHHHHHHEEECCCCCCCCCEECCCEECCCCCCCHHHHHHHHHHHCCCCCHHHH
MAGRLSGSAMHFDHDGRAATASVNFITAHDGFTLSDLVSYREKHNEANGEDNRDGHSNNH
HHHHCCCCEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
SDNFGVEGETDDPDILAARTQRRRNLIATLMLSQGTPLMLAGDEIGNTQGGNNNTYCQDS
CCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEECC
EIGWVDWAGRDDAFYAFCKAAIAFRKAHPILRQRRFLHSKPRFIDGAPDLYWRRTDGTPM
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCCCC
RQTDWDNPDLKIIVAEMRMASGTPAYIKREGALLVVLNVGGAADITLPTLDTGQWVRVFD
CCCCCCCCCEEEEEEEEHHCCCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCEEEEEC
SAQPDPTALPPGADPQIDANCVVVYELQPNTEGHNHAA
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLENFHVEAGAPNPLGATFDGGGVNFAVFSRHATQVMLCLFNEAGEETHLIAMPERDGHV
CCCCEEEECCCCCCCCCEECCCCEEEEEECCCHHHEEHHHHHCCCCCEEEEEECCCCCCE
WHGYVPGMGPGQQYGYRVHGPYAPEDGHRFNPYKLVLDPYAKRLTGQPIWNDALYGYVHG
EEEECCCCCCCHHCCEEECCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
KSDLTFDTRDSAPYMPRCIVTDPAFSWGADAAPRVPMDETVFYEAHVKGLTATHPGVEAP
CCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCCCCCC
GTYLGMASPPILDHLTDLGVTTVELLPVQAFVDDRFLVDKGLRNYWGYMTYGFFAPEPRY
CCEECCCCCHHHHHHHHCCCEEEEEEEEHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCC
MSNGSIAEFQQMVARYHSAGIEVILDVVYNHTAEGSEFGPTLAFRGLDNASYYRLTDDKR
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEECCCE
YYVNDAGCGNSLNFDNPFTLRMVMDSLRYWVEVMHIDGFRFDLCTSLGRTDGVFDRDGPF
EEEECCCCCCCCCCCCCEEHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCCCCCCCHH
FRAIRQDPVLNRVKLIAEPWDLGPDGYQLGAYQAPFGEWNDKFRDDTRRFWRGDAGMVAA
HHHHHCCCHHHHHHEEECCCCCCCCCEECCCEECCCCCCCHHHHHHHHHHHCCCCCHHHH
MAGRLSGSAMHFDHDGRAATASVNFITAHDGFTLSDLVSYREKHNEANGEDNRDGHSNNH
HHHHCCCCEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
SDNFGVEGETDDPDILAARTQRRRNLIATLMLSQGTPLMLAGDEIGNTQGGNNNTYCQDS
CCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCCCCCCCEEECC
EIGWVDWAGRDDAFYAFCKAAIAFRKAHPILRQRRFLHSKPRFIDGAPDLYWRRTDGTPM
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCCCC
RQTDWDNPDLKIIVAEMRMASGTPAYIKREGALLVVLNVGGAADITLPTLDTGQWVRVFD
CCCCCCCCCEEEEEEEEHHCCCCCCEEEECCCEEEEEECCCCCEEEECCCCCCCEEEEEC
SAQPDPTALPPGADPQIDANCVVVYELQPNTEGHNHAA
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]