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The map label for this gene is glyA

Identifier: 89055482

GI number: 89055482

Start: 3011924

End: 3013243

Strand: Direct

Name: glyA

Synonym: Jann_2991

Alternate gene names: 89055482

Gene position: 3011924-3013243 (Clockwise)

Preceding gene: 89055472

Following gene: 89055486

Centisome position: 69.75

GC content: 61.97

Gene sequence:

>1320_bases
ATGAACGCCCCCCATCGCGACGAGACAACCGCGTCCCACAGAGACGACGGATTTTTCACTGAAAGCCTGGAGTCCCGCGA
TCCCGAGATCTTCGCCGCCTCGCAAAAAGAGCTGGGACGCCAGCGCGACGAGATTGAGCTGATCGCGTCGGAGAACATCG
TGTCGGCCGCCGTGATGGAAGCCCAGGGCGGCGTGATGACCAACAAATACGCCGAGGGATATCCAGGGCGGCGCTATTAT
GGTGGCTGCCAATACGTTGATATCGCAGAGGAATTGGCGATTGATCGCGCCAAGCAGCTGTTCGGGTGCGACTTCGCCAA
CGTACAGCCCAACTCCGGGTCTCAGGCAAACCAGGGCGTGTTCACGGCCCTGCTGCAACCCGGCGACACGATCCTCGGCA
TGAGCCTTGATGCCGGCGGCCACCTCACCCACGGTGCACGCCCCAACCAATCGGGCAAGTGGTTCAACGCCGTGCAATAC
GGGGTGCGTGAAGGCGATCTGGAGATCGACTATGACCAGATCGCGGCGCTGGCCGCTGAGCACAAGCCCAAGATGATCAT
CGCAGGCGGCTCTGCCATTCCGCGGATCATCGATTTTGCCCGCATGCGCGAGATTGCCGACACGATTGGCGCATATCTCC
TTGTGGACATGGCACATTTCGCAGGAATGGTTGCGTCCGGCCATTACCCGTCGCCCTTCCCCCACGCCCATGTGGCAACG
ACCACGACGCACAAAACCCTGCGTGGCCCGCGCGGTGGCATGATCGTCACGAACGATGAGGCGATCGCCAAGAAGGTGAA
TTCCGCGATTTTCCCCGGTATCCAGGGCGGTCCCCTGATGCATGTGATTGCCGGTAAGGCTGTCGCGTTCGGCGAGGCGC
TGCGCCCTGAATTCAGGGATTATCAGACGCAGGTGATCGCCAACGCACAAGCGCTGGCAGCGCAGTTGATTAAGGGCGGT
CTGGACATCGTTACCGGCGGCACCGACACGCATTTGATGCTGGTGGATCTGCGCGCCAAAGGCGTTAAGGGCAACGCGAC
TGAAAAGGCCCTGGGCCGTGCCCACATCACCTGCAACAAGAACGGCATCCCCTTCGACACCGAAAAGCCGATGGTCACAT
CCGGTTTGCGCCTGGGCTCCCCCGCCGGGACCACCCGCGGGTTTGGCGAGGCGGAGTTCCGCCAGATCGCCGATTGGATC
GTGGAGGTCGTCGATGGCCTCGCGGCCAATGGGGAGGACGCCAATGACGCGGTCGAAGCCAAGGTCCGGGGGGAGGTGCA
GGCGCTTTGTGACCGCTTCCCGATCTATCCGAACCTGTGA

Upstream 100 bases:

>100_bases
GTTTTTCTCAACAACAGCGCCGCAATCCGGGCTTTCCCGATGCCTCAATCTGGCCTACATCCCCCTTGAATTCAACCACG
CCCAGTGCAGGAGCTTCCCC

Downstream 100 bases:

>100_bases
CACGGCTTTGAGGGGAGCCCGGAATTGATATACCATTGATATACCCCGCCGCGCGTTTGCTGTGTCTGGATCATGGTTAA
TGCGGGACACGAACAGGCCG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MNAPHRDETTASHRDDGFFTESLESRDPEIFAASQKELGRQRDEIELIASENIVSAAVMEAQGGVMTNKYAEGYPGRRYY
GGCQYVDIAEELAIDRAKQLFGCDFANVQPNSGSQANQGVFTALLQPGDTILGMSLDAGGHLTHGARPNQSGKWFNAVQY
GVREGDLEIDYDQIAALAAEHKPKMIIAGGSAIPRIIDFARMREIADTIGAYLLVDMAHFAGMVASGHYPSPFPHAHVAT
TTTHKTLRGPRGGMIVTNDEAIAKKVNSAIFPGIQGGPLMHVIAGKAVAFGEALRPEFRDYQTQVIANAQALAAQLIKGG
LDIVTGGTDTHLMLVDLRAKGVKGNATEKALGRAHITCNKNGIPFDTEKPMVTSGLRLGSPAGTTRGFGEAEFRQIADWI
VEVVDGLAANGEDANDAVEAKVRGEVQALCDRFPIYPNL

Sequences:

>Translated_439_residues
MNAPHRDETTASHRDDGFFTESLESRDPEIFAASQKELGRQRDEIELIASENIVSAAVMEAQGGVMTNKYAEGYPGRRYY
GGCQYVDIAEELAIDRAKQLFGCDFANVQPNSGSQANQGVFTALLQPGDTILGMSLDAGGHLTHGARPNQSGKWFNAVQY
GVREGDLEIDYDQIAALAAEHKPKMIIAGGSAIPRIIDFARMREIADTIGAYLLVDMAHFAGMVASGHYPSPFPHAHVAT
TTTHKTLRGPRGGMIVTNDEAIAKKVNSAIFPGIQGGPLMHVIAGKAVAFGEALRPEFRDYQTQVIANAQALAAQLIKGG
LDIVTGGTDTHLMLVDLRAKGVKGNATEKALGRAHITCNKNGIPFDTEKPMVTSGLRLGSPAGTTRGFGEAEFRQIADWI
VEVVDGLAANGEDANDAVEAKVRGEVQALCDRFPIYPNL
>Mature_439_residues
MNAPHRDETTASHRDDGFFTESLESRDPEIFAASQKELGRQRDEIELIASENIVSAAVMEAQGGVMTNKYAEGYPGRRYY
GGCQYVDIAEELAIDRAKQLFGCDFANVQPNSGSQANQGVFTALLQPGDTILGMSLDAGGHLTHGARPNQSGKWFNAVQY
GVREGDLEIDYDQIAALAAEHKPKMIIAGGSAIPRIIDFARMREIADTIGAYLLVDMAHFAGMVASGHYPSPFPHAHVAT
TTTHKTLRGPRGGMIVTNDEAIAKKVNSAIFPGIQGGPLMHVIAGKAVAFGEALRPEFRDYQTQVIANAQALAAQLIKGG
LDIVTGGTDTHLMLVDLRAKGVKGNATEKALGRAHITCNKNGIPFDTEKPMVTSGLRLGSPAGTTRGFGEAEFRQIADWI
VEVVDGLAANGEDANDAVEAKVRGEVQALCDRFPIYPNL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=419, Percent_Identity=47.0167064439141, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862352, Length=419, Percent_Identity=47.0167064439141, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862350, Length=419, Percent_Identity=47.0167064439141, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862348, Length=419, Percent_Identity=47.0167064439141, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862346, Length=419, Percent_Identity=46.0620525059666, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI22547186, Length=477, Percent_Identity=43.8155136268344, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI22547189, Length=462, Percent_Identity=41.7748917748918, Blast_Score=304, Evalue=9e-83,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=58.495145631068, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=412, Percent_Identity=46.3592233009709, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI25144729, Length=412, Percent_Identity=46.3592233009709, Blast_Score=347, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6319739, Length=431, Percent_Identity=43.8515081206497, Blast_Score=346, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6323087, Length=419, Percent_Identity=43.436754176611, Blast_Score=330, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24640005, Length=417, Percent_Identity=45.3237410071942, Blast_Score=354, Evalue=5e-98,
Organism=Drosophila melanogaster, GI221329721, Length=408, Percent_Identity=46.078431372549, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_JANSC (Q28N04)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510933.1
- ProteinModelPortal:   Q28N04
- SMR:   Q28N04
- STRING:   Q28N04
- GeneID:   3935461
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2991
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   DYDQIAA
- PhylomeDB:   Q28N04
- ProtClustDB:   PRK00011
- BioCyc:   JSP290400:JANN_2991-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 47046; Mature: 47046

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPHRDETTASHRDDGFFTESLESRDPEIFAASQKELGRQRDEIELIASENIVSAAVME
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCHHHHCCCCCCEEEEECCCHHHHHHHH
AQGGVMTNKYAEGYPGRRYYGGCQYVDIAEELAIDRAKQLFGCDFANVQPNSGSQANQGV
HCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
FTALLQPGDTILGMSLDAGGHLTHGARPNQSGKWFNAVQYGVREGDLEIDYDQIAALAAE
EEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEECHHHHHHHHHC
HKPKMIIAGGSAIPRIIDFARMREIADTIGAYLLVDMAHFAGMVASGHYPSPFPHAHVAT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TTTHKTLRGPRGGMIVTNDEAIAKKVNSAIFPGIQGGPLMHVIAGKAVAFGEALRPEFRD
CCCHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHHCHHHHH
YQTQVIANAQALAAQLIKGGLDIVTGGTDTHLMLVDLRAKGVKGNATEKALGRAHITCNK
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCCCCCHHHHCCCEEEEECC
NGIPFDTEKPMVTSGLRLGSPAGTTRGFGEAEFRQIADWIVEVVDGLAANGEDANDAVEA
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
KVRGEVQALCDRFPIYPNL
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAPHRDETTASHRDDGFFTESLESRDPEIFAASQKELGRQRDEIELIASENIVSAAVME
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCHHHHCCCCCCEEEEECCCHHHHHHHH
AQGGVMTNKYAEGYPGRRYYGGCQYVDIAEELAIDRAKQLFGCDFANVQPNSGSQANQGV
HCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
FTALLQPGDTILGMSLDAGGHLTHGARPNQSGKWFNAVQYGVREGDLEIDYDQIAALAAE
EEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEECHHHHHHHHHC
HKPKMIIAGGSAIPRIIDFARMREIADTIGAYLLVDMAHFAGMVASGHYPSPFPHAHVAT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
TTTHKTLRGPRGGMIVTNDEAIAKKVNSAIFPGIQGGPLMHVIAGKAVAFGEALRPEFRD
CCCHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHHCHHHHH
YQTQVIANAQALAAQLIKGGLDIVTGGTDTHLMLVDLRAKGVKGNATEKALGRAHITCNK
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCCCCCHHHHCCCEEEEECC
NGIPFDTEKPMVTSGLRLGSPAGTTRGFGEAEFRQIADWIVEVVDGLAANGEDANDAVEA
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
KVRGEVQALCDRFPIYPNL
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA