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The map label for this gene is mltB [H]

Identifier: 89055359

GI number: 89055359

Start: 2889862

End: 2891139

Strand: Direct

Name: mltB [H]

Synonym: Jann_2868

Alternate gene names: 89055359

Gene position: 2889862-2891139 (Clockwise)

Preceding gene: 89055356

Following gene: 89055367

Centisome position: 66.93

GC content: 66.67

Gene sequence:

>1278_bases
ATGCGGATCACACGGCGAACATTTGGGGCGGGTGCAGGGTCTTTGGCCCTGAGCGGCTGTGTCGGCGGCCTTGGCGGCGG
GCTCGGTGCTGGGTTGGGCGGCGGGATCGGGACGCCGGGCGATGGCGGGCCGTCCACGGATCCGGACTTCAGGCCGCAAC
CCAATGCCTCCTACGACGCCTGGCTGGACGGCTTTCGGGATCGGGCGCAGGCGGCGGGGATCTCGGAGGATGCCCTGACG
CGCGGATTGCGCGGTGTGGGTTACTTGCCTGGCGTGGTCGAACGGGACCGCAATCAAACGGAATTTCGCCGCTCGACCGA
GGATTATCTGGCCCTTGTGGCGGGCGACGACGATGTCAGCCTGGGGCGGTCGCGCTTCAACGCGCAGCGCAGCGTGCTGG
CGGATGTGGAGGCGCGGTACGGCGTCCCCGCCGAGGTGTGCTGTGCGGTCTGGGGTGTTGAGAGCCGCTATGGCACGCGG
TTGGGGGACATTCCGGTGATCTCGGCCGTGTCCACGCTGGCCTGGGAAGGGCGGCGCGGGGACTTCTTTGAAGCGCAGAC
CATTGCCGCCCTCCGCATCATCCAGAATGGCGACACGACCACCGACCGCATGCTGGGATCCTGGGCCGGAGCGATGGGGC
ATACGCAATTGATTCCAACGACTTACGAGGAATATGCCGTGGACTTCCGGGGCGATGGGCGGCGCGACATCTGGACCAAT
GATCCCACCGATGCCTTCGCCTCCACGGCCAGCTATTTCAGCCGGTTCAACTGGCGCACGGGTCAGCCCTGGGGCATGGA
GGTGGAGCTTCCGTCCGGGTTCAGCGCCGCCACCGGGCGCAACAATCGCCGCTCTGTGGCGAGTTGGAGCGGCCTTGGGG
TTCGGCGCGCGGGGGGCGGTGCGTTGCCGGATCATGGGCAAGCGGCCATTCATGCCCCCGGCGGCGCGGGCGCACCTGCA
TGGATTTTGTATCACAATTTCAACGTTATACTCCGCTATAACGCCTCCACCAATTACGGGATCGGCGTGGGATACCTCTC
GGATCGGATCGCGGGCGGTGGGCCGTTGCGGCAGAGTTTCGGGGCCGATGCCAGCGGGCTGACGCAGGCGCAGCGGAGAG
AATTACAGGAGCGCCTCAACAGCGCCGGTTATGATGCGGGCACGCCCGATGGGGTCATCGGATCGGGCACCGAGGCCGCG
ATTGAGGCCTATCAATCGGCCAACGGATTGCCGGTGACCGGAGAGCCCTCGGCGAGCCTTCTGCGGCGATTGCGGTAG

Upstream 100 bases:

>100_bases
GCAGATGATTGCTCGCAAGCCCGCAAGAGCTTCGGTAAGCTTGCAAAATGCGCTGATGCGCCGATACCTCTGGGCAAACC
GAACATAAAGGGCTGACGCG

Downstream 100 bases:

>100_bases
CGATTTGGCGTGTAGTGAACCCGTAGTGATACCGTAGTGATACCGTAGTTTTTCTGTAACTACACGATTTTTGCACTCAA
TCCGTCAGAATCTCAGCCAA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRITRRTFGAGAGSLALSGCVGGLGGGLGAGLGGGIGTPGDGGPSTDPDFRPQPNASYDAWLDGFRDRAQAAGISEDALT
RGLRGVGYLPGVVERDRNQTEFRRSTEDYLALVAGDDDVSLGRSRFNAQRSVLADVEARYGVPAEVCCAVWGVESRYGTR
LGDIPVISAVSTLAWEGRRGDFFEAQTIAALRIIQNGDTTTDRMLGSWAGAMGHTQLIPTTYEEYAVDFRGDGRRDIWTN
DPTDAFASTASYFSRFNWRTGQPWGMEVELPSGFSAATGRNNRRSVASWSGLGVRRAGGGALPDHGQAAIHAPGGAGAPA
WILYHNFNVILRYNASTNYGIGVGYLSDRIAGGGPLRQSFGADASGLTQAQRRELQERLNSAGYDAGTPDGVIGSGTEAA
IEAYQSANGLPVTGEPSASLLRRLR

Sequences:

>Translated_425_residues
MRITRRTFGAGAGSLALSGCVGGLGGGLGAGLGGGIGTPGDGGPSTDPDFRPQPNASYDAWLDGFRDRAQAAGISEDALT
RGLRGVGYLPGVVERDRNQTEFRRSTEDYLALVAGDDDVSLGRSRFNAQRSVLADVEARYGVPAEVCCAVWGVESRYGTR
LGDIPVISAVSTLAWEGRRGDFFEAQTIAALRIIQNGDTTTDRMLGSWAGAMGHTQLIPTTYEEYAVDFRGDGRRDIWTN
DPTDAFASTASYFSRFNWRTGQPWGMEVELPSGFSAATGRNNRRSVASWSGLGVRRAGGGALPDHGQAAIHAPGGAGAPA
WILYHNFNVILRYNASTNYGIGVGYLSDRIAGGGPLRQSFGADASGLTQAQRRELQERLNSAGYDAGTPDGVIGSGTEAA
IEAYQSANGLPVTGEPSASLLRRLR
>Mature_425_residues
MRITRRTFGAGAGSLALSGCVGGLGGGLGAGLGGGIGTPGDGGPSTDPDFRPQPNASYDAWLDGFRDRAQAAGISEDALT
RGLRGVGYLPGVVERDRNQTEFRRSTEDYLALVAGDDDVSLGRSRFNAQRSVLADVEARYGVPAEVCCAVWGVESRYGTR
LGDIPVISAVSTLAWEGRRGDFFEAQTIAALRIIQNGDTTTDRMLGSWAGAMGHTQLIPTTYEEYAVDFRGDGRRDIWTN
DPTDAFASTASYFSRFNWRTGQPWGMEVELPSGFSAATGRNNRRSVASWSGLGVRRAGGGALPDHGQAAIHAPGGAGAPA
WILYHNFNVILRYNASTNYGIGVGYLSDRIAGGGPLRQSFGADASGLTQAQRRELQERLNSAGYDAGTPDGVIGSGTEAA
IEAYQSANGLPVTGEPSASLLRRLR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=242, Percent_Identity=31.8181818181818, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44851; Mature: 44851

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITRRTFGAGAGSLALSGCVGGLGGGLGAGLGGGIGTPGDGGPSTDPDFRPQPNASYDA
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WLDGFRDRAQAAGISEDALTRGLRGVGYLPGVVERDRNQTEFRRSTEDYLALVAGDDDVS
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHHCCCCEEEEEECCCCHH
LGRSRFNAQRSVLADVEARYGVPAEVCCAVWGVESRYGTRLGDIPVISAVSTLAWEGRRG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHCCCCCC
DFFEAQTIAALRIIQNGDTTTDRMLGSWAGAMGHTQLIPTTYEEYAVDFRGDGRRDIWTN
CCHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCEECCCCHHHHEEECCCCCCCCCCCC
DPTDAFASTASYFSRFNWRTGQPWGMEVELPSGFSAATGRNNRRSVASWSGLGVRRAGGG
CCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCEEECCCC
ALPDHGQAAIHAPGGAGAPAWILYHNFNVILRYNASTNYGIGVGYLSDRIAGGGPLRQSF
CCCCCCCEEEECCCCCCCCEEEEEECCEEEEEECCCCCCCEEEHHHHHHCCCCCCHHHHH
GADASGLTQAQRRELQERLNSAGYDAGTPDGVIGSGTEAAIEAYQSANGLPVTGEPSASL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHH
LRRLR
HHHCC
>Mature Secondary Structure
MRITRRTFGAGAGSLALSGCVGGLGGGLGAGLGGGIGTPGDGGPSTDPDFRPQPNASYDA
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WLDGFRDRAQAAGISEDALTRGLRGVGYLPGVVERDRNQTEFRRSTEDYLALVAGDDDVS
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHHCCCCEEEEEECCCCHH
LGRSRFNAQRSVLADVEARYGVPAEVCCAVWGVESRYGTRLGDIPVISAVSTLAWEGRRG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHCCCCCC
DFFEAQTIAALRIIQNGDTTTDRMLGSWAGAMGHTQLIPTTYEEYAVDFRGDGRRDIWTN
CCHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCEECCCCHHHHEEECCCCCCCCCCCC
DPTDAFASTASYFSRFNWRTGQPWGMEVELPSGFSAATGRNNRRSVASWSGLGVRRAGGG
CCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHCCCCEEECCCC
ALPDHGQAAIHAPGGAGAPAWILYHNFNVILRYNASTNYGIGVGYLSDRIAGGGPLRQSF
CCCCCCCEEEECCCCCCCCEEEEEECCEEEEEECCCCCCCEEEHHHHHHCCCCCCHHHHH
GADASGLTQAQRRELQERLNSAGYDAGTPDGVIGSGTEAAIEAYQSANGLPVTGEPSASL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHH
LRRLR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]