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The map label for this gene is vsr [H]

Identifier: 89055348

GI number: 89055348

Start: 2877430

End: 2877900

Strand: Reverse

Name: vsr [H]

Synonym: Jann_2857

Alternate gene names: 89055348

Gene position: 2877900-2877430 (Counterclockwise)

Preceding gene: 89055351

Following gene: 89055346

Centisome position: 66.65

GC content: 56.48

Gene sequence:

>471_bases
ATGGTAGACATCGTTTCGGCGGAAACGCGATCGAGGATGATGGCTTCGATCCGCCGAAGGAACACCAAACCGGAAATGGT
GGTTCGCCAAGAACTGCATGCGGCAGGATTCAGGTTCCGGCTGGACGTCCGATCTTTGCCTGGTTCTCCCGATATCGTGC
TGCCGAAATGGCGAACGGCCATTTTTGTCCACGGTTGCTTCTGGCATCGTCATGCCGGGTGCAAGTATGCGAGCACTCCA
GCGACACGTCCCAAATTCTGGCAGGCGAAGTTCGAAGCAAATGTCCGGCGGGATTGCAGCAACGAAAGAGCCTTAATCGA
AATGGGCTGGAAGGTCGCCATTGTATGGGAATGTGGGCTCGCACAAGGCGTCAGGGCCGACACGATCTGCGGTCTCTTCG
CATTTATTCGGCATCCAAGCCCGTCTCCGACCAGATTGTTTTTCCCGGAAACGCCGCCGCGTCCCATCTGA

Upstream 100 bases:

>100_bases
CGATAATGCCAAAGCTTCCGTCCATTCATTTCACCTTTCTCGAGCAACCTTCTAAACTGAAGACTGCAGGCCGTGCCAGG
GGCAAAGGGGGCACTCATCG

Downstream 100 bases:

>100_bases
GTGGAGGATTTCGGCGGGGCTGGGGGAGCGGGCTCTGGTCGTGACCGATTGTCAAATCAAACTTTTTCGCGCGGATCGCA
TCTTCGCCGGTATCAACATC

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MVDIVSAETRSRMMASIRRRNTKPEMVVRQELHAAGFRFRLDVRSLPGSPDIVLPKWRTAIFVHGCFWHRHAGCKYASTP
ATRPKFWQAKFEANVRRDCSNERALIEMGWKVAIVWECGLAQGVRADTICGLFAFIRHPSPSPTRLFFPETPPRPI

Sequences:

>Translated_156_residues
MVDIVSAETRSRMMASIRRRNTKPEMVVRQELHAAGFRFRLDVRSLPGSPDIVLPKWRTAIFVHGCFWHRHAGCKYASTP
ATRPKFWQAKFEANVRRDCSNERALIEMGWKVAIVWECGLAQGVRADTICGLFAFIRHPSPSPTRLFFPETPPRPI
>Mature_156_residues
MVDIVSAETRSRMMASIRRRNTKPEMVVRQELHAAGFRFRLDVRSLPGSPDIVLPKWRTAIFVHGCFWHRHAGCKYASTP
ATRPKFWQAKFEANVRRDCSNERALIEMGWKVAIVWECGLAQGVRADTICGLFAFIRHPSPSPTRLFFPETPPRPI

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=120, Percent_Identity=45, Blast_Score=106, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17905; Mature: 17905

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDIVSAETRSRMMASIRRRNTKPEMVVRQELHAAGFRFRLDVRSLPGSPDIVLPKWRTA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCEECCCCCEE
IFVHGCFWHRHAGCKYASTPATRPKFWQAKFEANVRRDCSNERALIEMGWKVAIVWECGL
EEEEEHHHHHCCCCEECCCCCCCCCEEHHHHHCCHHCCCCCCCEEEECCCEEEEEEECCC
AQGVRADTICGLFAFIRHPSPSPTRLFFPETPPRPI
CCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MVDIVSAETRSRMMASIRRRNTKPEMVVRQELHAAGFRFRLDVRSLPGSPDIVLPKWRTA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCEECCCCCEE
IFVHGCFWHRHAGCKYASTPATRPKFWQAKFEANVRRDCSNERALIEMGWKVAIVWECGL
EEEEEHHHHHCCCCEECCCCCCCCCEEHHHHHCCHHCCCCCCCEEEECCCEEEEEEECCC
AQGVRADTICGLFAFIRHPSPSPTRLFFPETPPRPI
CCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]