Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 89055292

GI number: 89055292

Start: 2814008

End: 2814790

Strand: Reverse

Name: rlmB [H]

Synonym: Jann_2801

Alternate gene names: 89055292

Gene position: 2814790-2814008 (Counterclockwise)

Preceding gene: 89055298

Following gene: 89055291

Centisome position: 65.19

GC content: 66.67

Gene sequence:

>783_bases
ATGGCCAGGAAACCAACCTGGGTGATCGATAAGGAGCGGGGAAAGCGCGCAGCGGCGGCAGAGACCATCTGGTTATTCGG
CCTGCATGCGGTGCGCGACGCGCTGGCCAACCCGGACCGTCAGGTTCTGCGGCTGGTGGTGTCGCTTAACGCGCAGACCA
AGCTGGCCGATGCCATTGCCGCGCGGGGGCTGGAGGCGGAAATTGCCGATCCGCGCAAATTTCCGGTGCCACTCGACCCG
GGCTCGGTCCATCAGGGCGCGGCGGTGGAGGTCAAGCCGTTGGATTGGGGTGACTGGCGGGCCCTGGCGCGGGGCGATGG
GGCGGAGCGGTCTGTCGTCGTGCTGCTGGACCGGGTCAGTGATCCGCATAATGTGGGTGCGATCCTGCGCTCTGCCGAGG
TGTTCGGCGCGCGGGCTGTGATCGCGCCCGCCCGCCATTCCGCACCGGAAACGGGTGCGCTCGCCAAAACCGCCTCCGGC
GCGCTGGAGCGGATGCCCTATGCGCGCATCGGCAATCTGGGGGACGCGATGGAGGCGCTGAAGGCCGATGGCTTTTGGAT
GGTCGGATTGGCAGAGGAGGGCGCGGCCCCGATCGACGCGGTCGCCGCCCGTCACACAGACAGCAACATCGCGTTGGTTT
TGGGGGCGGAGGGACCCGGATTGCGGGAGCGAACCCGAGGCCTCTGCGACGAATTGGCCAAAATCCCGTTCGCGCGCGAT
TTTGGGTCGCTCAATGTCTCAAATGCAGCCGCCGTGGCCCTATATGCCCTAACGAAAGGGTAG

Upstream 100 bases:

>100_bases
AATATCGACGCGTGCGGGGGGGGTCAAGTCCCAATGCAGCCTTGCGCTTGCCCCATTGCCTGCGCAGTAAGGAGGCAACA
CGAAAGAGGGGTGAGGGTTC

Downstream 100 bases:

>100_bases
CTCTATGCCACATGCCACGAAGATCGCGACCTGCTGCTATTGCGGGCGACGTCAGACACTGAGGCCCACCGCGCGCGACG
GGCATGAGCTGGCCTGCGGC

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MARKPTWVIDKERGKRAAAAETIWLFGLHAVRDALANPDRQVLRLVVSLNAQTKLADAIAARGLEAEIADPRKFPVPLDP
GSVHQGAAVEVKPLDWGDWRALARGDGAERSVVVLLDRVSDPHNVGAILRSAEVFGARAVIAPARHSAPETGALAKTASG
ALERMPYARIGNLGDAMEALKADGFWMVGLAEEGAAPIDAVAARHTDSNIALVLGAEGPGLRERTRGLCDELAKIPFARD
FGSLNVSNAAAVALYALTKG

Sequences:

>Translated_260_residues
MARKPTWVIDKERGKRAAAAETIWLFGLHAVRDALANPDRQVLRLVVSLNAQTKLADAIAARGLEAEIADPRKFPVPLDP
GSVHQGAAVEVKPLDWGDWRALARGDGAERSVVVLLDRVSDPHNVGAILRSAEVFGARAVIAPARHSAPETGALAKTASG
ALERMPYARIGNLGDAMEALKADGFWMVGLAEEGAAPIDAVAARHTDSNIALVLGAEGPGLRERTRGLCDELAKIPFARD
FGSLNVSNAAAVALYALTKG
>Mature_259_residues
ARKPTWVIDKERGKRAAAAETIWLFGLHAVRDALANPDRQVLRLVVSLNAQTKLADAIAARGLEAEIADPRKFPVPLDPG
SVHQGAAVEVKPLDWGDWRALARGDGAERSVVVLLDRVSDPHNVGAILRSAEVFGARAVIAPARHSAPETGALAKTASGA
LERMPYARIGNLGDAMEALKADGFWMVGLAEEGAAPIDAVAARHTDSNIALVLGAEGPGLRERTRGLCDELAKIPFARDF
GSLNVSNAAAVALYALTKG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=259, Percent_Identity=30.1158301158301, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=34.7107438016529, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17555908, Length=253, Percent_Identity=26.4822134387352, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324775, Length=255, Percent_Identity=24.7058823529412, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27441; Mature: 27309

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKPTWVIDKERGKRAAAAETIWLFGLHAVRDALANPDRQVLRLVVSLNAQTKLADAIA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHH
ARGLEAEIADPRKFPVPLDPGSVHQGAAVEVKPLDWGDWRALARGDGAERSVVVLLDRVS
HCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCEEEEEEECCC
DPHNVGAILRSAEVFGARAVIAPARHSAPETGALAKTASGALERMPYARIGNLGDAMEAL
CCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCHHHHHHHH
KADGFWMVGLAEEGAAPIDAVAARHTDSNIALVLGAEGPGLRERTRGLCDELAKIPFARD
HCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
FGSLNVSNAAAVALYALTKG
CCCCCCCCHHEEEEEEEECC
>Mature Secondary Structure 
ARKPTWVIDKERGKRAAAAETIWLFGLHAVRDALANPDRQVLRLVVSLNAQTKLADAIA
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHH
ARGLEAEIADPRKFPVPLDPGSVHQGAAVEVKPLDWGDWRALARGDGAERSVVVLLDRVS
HCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCEEEEEEECCC
DPHNVGAILRSAEVFGARAVIAPARHSAPETGALAKTASGALERMPYARIGNLGDAMEAL
CCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCHHHHHHHH
KADGFWMVGLAEEGAAPIDAVAARHTDSNIALVLGAEGPGLRERTRGLCDELAKIPFARD
HCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
FGSLNVSNAAAVALYALTKG
CCCCCCCCHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA