Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 89055260

GI number: 89055260

Start: 2784986

End: 2785969

Strand: Reverse

Name: mraW

Synonym: Jann_2769

Alternate gene names: 89055260

Gene position: 2785969-2784986 (Counterclockwise)

Preceding gene: 89055261

Following gene: 89055259

Centisome position: 64.52

GC content: 61.48

Gene sequence:

>984_bases
ATGACAGACGAGATCGACAAAGCCCCGCACATCCCGGTTCTCTTGGGCGCCATCCTGCGCGAAGTGGCGCCGGTTTCTGG
CGTATGGTTGGACGGGACATTCGGCGCTGGTGGATACGCCCGTGGCTTGCTTGAGGGTGGGGCCGATACGGTGATTGGTG
TGGACCGCGATCCCCTGGCCTTCGAGATGGCTGCAGATTGGGCCGGGTCCTACGGCGATGCCCTGCGTCTGAAAGAGGGC
ACGTTTTCGAACCTCGATACCTTGGCAGACGAGCCGTTGGACGGCGTCGCATTGGATCTGGGCGTGTCGTCCATGCAGCT
GGATCTGGCTGAGCGTGGGTTCTCCTTCATGCGCGACGGGCCGCTGGATATGCGGATGGGTCAAATTGGTGTTTCTGCTG
AAGATTTGGTTAACGACGCGCCCGAAAAACTTCTGGCCGATATCCTATTCCAATATGGGGAGGAGCGTGCTGCGCGCCGG
ATTGCCAAGGCAATCGTCGCAGAGCGGTTGAAGTGTCGCATCAACTCCACGCTGCAACTGGCTGGTATCGTTGAGAAGTG
TTTGCCAAGTAAGAAGCCGGGGCAAAGCCATCCCGCGACGCGCAGCTTCCAGGCGATCCGTATTGCGGTCAACGATGAAT
TTGGTCAACTGGCCCAAGGCCTGATGGCGGCCGAACGCGCCCTTAGGCCTGGCGGCAAACTCGCAGTTGTCACGTTTCAC
TCATCCGAGGACCGGATCGTCAAGAAATTCATGTCCGAGCGCTCCAGCACCGGGGGCGGGGGCTCTCGCTATGCGCCGGA
GGCCGCGGCAAAGGTACCCGGTTTCACGCTCACCCCCCGCAAAGCCATTCCGCCAGACGCCGATGAAATCGCGCGCAACA
CCCGCGCCCGCTCGGCCAAACTGCGCATCGCCACACGTACCGATGCGCCCGCGCAGCCCGTGGCGCCAGAGACCCTCGGG
TTGCCACAATTGGAGGGTTTTTGA

Upstream 100 bases:

>100_bases
TGGCCGCTCTGCTGGATCAGGCCGCGGAAGGCAAAGAATTCTTCAATCCCATTTCACTGGCGAACCCGCCCAAACCCACC
CCGGTGACAGACAATGCCGA

Downstream 100 bases:

>100_bases
GATGCGCTTCATCACGTTGATCGCCATTATCATCGTTGTGGCCTTGGGCAACTGGGCCTACCATCAGACAATCCAAACCC
AGATGACGGACCGTGACGTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTDEIDKAPHIPVLLGAILREVAPVSGVWLDGTFGAGGYARGLLEGGADTVIGVDRDPLAFEMAADWAGSYGDALRLKEG
TFSNLDTLADEPLDGVALDLGVSSMQLDLAERGFSFMRDGPLDMRMGQIGVSAEDLVNDAPEKLLADILFQYGEERAARR
IAKAIVAERLKCRINSTLQLAGIVEKCLPSKKPGQSHPATRSFQAIRIAVNDEFGQLAQGLMAAERALRPGGKLAVVTFH
SSEDRIVKKFMSERSSTGGGGSRYAPEAAAKVPGFTLTPRKAIPPDADEIARNTRARSAKLRIATRTDAPAQPVAPETLG
LPQLEGF

Sequences:

>Translated_327_residues
MTDEIDKAPHIPVLLGAILREVAPVSGVWLDGTFGAGGYARGLLEGGADTVIGVDRDPLAFEMAADWAGSYGDALRLKEG
TFSNLDTLADEPLDGVALDLGVSSMQLDLAERGFSFMRDGPLDMRMGQIGVSAEDLVNDAPEKLLADILFQYGEERAARR
IAKAIVAERLKCRINSTLQLAGIVEKCLPSKKPGQSHPATRSFQAIRIAVNDEFGQLAQGLMAAERALRPGGKLAVVTFH
SSEDRIVKKFMSERSSTGGGGSRYAPEAAAKVPGFTLTPRKAIPPDADEIARNTRARSAKLRIATRTDAPAQPVAPETLG
LPQLEGF
>Mature_326_residues
TDEIDKAPHIPVLLGAILREVAPVSGVWLDGTFGAGGYARGLLEGGADTVIGVDRDPLAFEMAADWAGSYGDALRLKEGT
FSNLDTLADEPLDGVALDLGVSSMQLDLAERGFSFMRDGPLDMRMGQIGVSAEDLVNDAPEKLLADILFQYGEERAARRI
AKAIVAERLKCRINSTLQLAGIVEKCLPSKKPGQSHPATRSFQAIRIAVNDEFGQLAQGLMAAERALRPGGKLAVVTFHS
SEDRIVKKFMSERSSTGGGGSRYAPEAAAKVPGFTLTPRKAIPPDADEIARNTRARSAKLRIATRTDAPAQPVAPETLGL
PQLEGF

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=39.1044776119403, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=42.7807486631016, Blast_Score=131, Evalue=6e-31,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=42.4528301886792, Blast_Score=202, Evalue=3e-53,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=41.6666666666667, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=41.6666666666667, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_JANSC (Q28NM6)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510711.1
- ProteinModelPortal:   Q28NM6
- SMR:   Q28NM6
- STRING:   Q28NM6
- GeneID:   3935233
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2769
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q28NM6
- ProtClustDB:   PRK00050
- BioCyc:   JSP290400:JANN_2769-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34865; Mature: 34734

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 82-82 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEIDKAPHIPVLLGAILREVAPVSGVWLDGTFGAGGYARGLLEGGADTVIGVDRDPLA
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCC
FEMAADWAGSYGDALRLKEGTFSNLDTLADEPLDGVALDLGVSSMQLDLAERGFSFMRDG
HHHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEECCCCHHHHHHHHHCHHHHCCC
PLDMRMGQIGVSAEDLVNDAPEKLLADILFQYGEERAARRIAKAIVAERLKCRINSTLQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHH
AGIVEKCLPSKKPGQSHPATRSFQAIRIAVNDEFGQLAQGLMAAERALRPGGKLAVVTFH
HHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
SSEDRIVKKFMSERSSTGGGGSRYAPEAAAKVPGFTLTPRKAIPPDADEIARNTRARSAK
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCEE
LRIATRTDAPAQPVAPETLGLPQLEGF
EEEEECCCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
TDEIDKAPHIPVLLGAILREVAPVSGVWLDGTFGAGGYARGLLEGGADTVIGVDRDPLA
CCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCC
FEMAADWAGSYGDALRLKEGTFSNLDTLADEPLDGVALDLGVSSMQLDLAERGFSFMRDG
HHHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEECCCCHHHHHHHHHCHHHHCCC
PLDMRMGQIGVSAEDLVNDAPEKLLADILFQYGEERAARRIAKAIVAERLKCRINSTLQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHH
AGIVEKCLPSKKPGQSHPATRSFQAIRIAVNDEFGQLAQGLMAAERALRPGGKLAVVTFH
HHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
SSEDRIVKKFMSERSSTGGGGSRYAPEAAAKVPGFTLTPRKAIPPDADEIARNTRARSAK
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEECCCCCCCCCHHHHHHHHHHCCEE
LRIATRTDAPAQPVAPETLGLPQLEGF
EEEEECCCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA