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The map label for this gene is psdht [H]

Identifier: 89055229

GI number: 89055229

Start: 2751573

End: 2752526

Strand: Direct

Name: psdht [H]

Synonym: Jann_2738

Alternate gene names: 89055229

Gene position: 2751573-2752526 (Clockwise)

Preceding gene: 89055228

Following gene: 89055230

Centisome position: 63.72

GC content: 64.36

Gene sequence:

>954_bases
ATGATCCATCCAAATTCAGATGCCATCTCGGCTGCCGCCCCGCTGATCGCGCCCCATATCCGCACAACCCCGATTCTGAC
CGTTGAAGCGGCTGCGCTATCGCTCCCTGTTCCGATCACGCTGAAGCTGGAGCACACTCAGATCACCGGCTCGTTCAAAC
TGCGCGGCGCGTTCTACAACATGCTGACCCGCGATGTGCCCGCGGCTGGTGTCGTTGCGGCCTCCGGCGGGAACCACGGC
GCGGCTGTGGCCTATGCGGCCACGGCGCTGGGGCATAAAAGCCGCATCTTCGTGCCCGCCACCATCGCCAAGGAAGAAAA
ACTCAAACGGATGAGGGATTTCGGGGCAGAGGTCGTCCTGACCGAAGGCTCTGTCGGGGCCTGTATGGAGGACTACGCGG
CCTACGCCGAGACGTCCGGCGCGCTCTCTGTCCATCCCTACGATACGATCCCCACGTTGACGGGCCAGGGCACGCTCGCG
CGCGAAATCGAACAGCAGATGACGGAAGCCGATCTGGGCGGCATCGACACCATCCTCGTCTCGACCGGGGGCGGCGGGCT
GATTGGCGGTATCTCCGCGTGGTTCCGCGACCGGATCAAGGTCGTCTCGATCGAGACAGAAGGGACAAATACGCTAGAGA
GATCCCTGCGGGAGGGACCGGATCTCGACGTGCCCGCCAGCGGGATTGCGGCGGGATCACTGGGGGGGCCGCGCCTGGGG
ATCGACAGCTACGCGTTGATCAAGGCCTTCGTGGATGAGGCGATCATCTTACCCGACACAGAGGTTTTCACCGCCGCCAA
GCGATTGTGGGACGGCACGCGCCTGATCGGAGAGCCCGGCAGCGCCGTGGCCCTCGCCGCCCTCACCTCCGGCGCTTACG
TGCCCGAAAAGGACGAACGCGTCTGCGTGGTCCTTTGCGGCGGCAATGCAGAGCCCGATTGGTTCGCGACATGA

Upstream 100 bases:

>100_bases
ATAGCGCAAAGGCTTGCACTGAAGAGGGTTGACGCGCCTGCAAACGGCGCCGTTTCTCCACCCGATTGACGCGGCCCGCC
GCCGCGGTCATCGTCCGGTC

Downstream 100 bases:

>100_bases
GGGGGACGGCCATGGCCGCCCTCCTCTGCCTCCCGTCCGCTGCAATCGCACAATCTGATCTCAGCGCAGAGCTGGCCGAC
ACACTCGCGCCCGTGGCGGA

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIHPNSDAISAAAPLIAPHIRTTPILTVEAAALSLPVPITLKLEHTQITGSFKLRGAFYNMLTRDVPAAGVVAASGGNHG
AAVAYAATALGHKSRIFVPATIAKEEKLKRMRDFGAEVVLTEGSVGACMEDYAAYAETSGALSVHPYDTIPTLTGQGTLA
REIEQQMTEADLGGIDTILVSTGGGGLIGGISAWFRDRIKVVSIETEGTNTLERSLREGPDLDVPASGIAAGSLGGPRLG
IDSYALIKAFVDEAIILPDTEVFTAAKRLWDGTRLIGEPGSAVALAALTSGAYVPEKDERVCVVLCGGNAEPDWFAT

Sequences:

>Translated_317_residues
MIHPNSDAISAAAPLIAPHIRTTPILTVEAAALSLPVPITLKLEHTQITGSFKLRGAFYNMLTRDVPAAGVVAASGGNHG
AAVAYAATALGHKSRIFVPATIAKEEKLKRMRDFGAEVVLTEGSVGACMEDYAAYAETSGALSVHPYDTIPTLTGQGTLA
REIEQQMTEADLGGIDTILVSTGGGGLIGGISAWFRDRIKVVSIETEGTNTLERSLREGPDLDVPASGIAAGSLGGPRLG
IDSYALIKAFVDEAIILPDTEVFTAAKRLWDGTRLIGEPGSAVALAALTSGAYVPEKDERVCVVLCGGNAEPDWFAT
>Mature_317_residues
MIHPNSDAISAAAPLIAPHIRTTPILTVEAAALSLPVPITLKLEHTQITGSFKLRGAFYNMLTRDVPAAGVVAASGGNHG
AAVAYAATALGHKSRIFVPATIAKEEKLKRMRDFGAEVVLTEGSVGACMEDYAAYAETSGALSVHPYDTIPTLTGQGTLA
REIEQQMTEADLGGIDTILVSTGGGGLIGGISAWFRDRIKVVSIETEGTNTLERSLREGPDLDVPASGIAAGSLGGPRLG
IDSYALIKAFVDEAIILPDTEVFTAAKRLWDGTRLIGEPGSAVALAALTSGAYVPEKDERVCVVLCGGNAEPDWFAT

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=306, Percent_Identity=30.718954248366, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI19923959, Length=303, Percent_Identity=26.4026402640264, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1790207, Length=295, Percent_Identity=31.864406779661, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789505, Length=245, Percent_Identity=30.2040816326531, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17508781, Length=319, Percent_Identity=29.7805642633229, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71991565, Length=308, Percent_Identity=33.4415584415584, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537387, Length=275, Percent_Identity=28.7272727272727, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320930, Length=313, Percent_Identity=30.3514376996805, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322631, Length=336, Percent_Identity=28.2738095238095, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319788, Length=270, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322024, Length=190, Percent_Identity=27.3684210526316, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355833, Length=314, Percent_Identity=26.4331210191083, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 32996; Mature: 32996

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00626 RCC1_2 ; PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHPNSDAISAAAPLIAPHIRTTPILTVEAAALSLPVPITLKLEHTQITGSFKLRGAFYN
CCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHEECCCEEEEEEEEEEEECEEEEHHHHHH
MLTRDVPAAGVVAASGGNHGAAVAYAATALGHKSRIFVPATIAKEEKLKRMRDFGAEVVL
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCEEEE
TEGSVGACMEDYAAYAETSGALSVHPYDTIPTLTGQGTLAREIEQQMTEADLGGIDTILV
ECCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
STGGGGLIGGISAWFRDRIKVVSIETEGTNTLERSLREGPDLDVPASGIAAGSLGGPRLG
ECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
IDSYALIKAFVDEAIILPDTEVFTAAKRLWDGTRLIGEPGSAVALAALTSGAYVPEKDER
CCHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCC
VCVVLCGGNAEPDWFAT
EEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIHPNSDAISAAAPLIAPHIRTTPILTVEAAALSLPVPITLKLEHTQITGSFKLRGAFYN
CCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHEECCCEEEEEEEEEEEECEEEEHHHHHH
MLTRDVPAAGVVAASGGNHGAAVAYAATALGHKSRIFVPATIAKEEKLKRMRDFGAEVVL
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCEEEE
TEGSVGACMEDYAAYAETSGALSVHPYDTIPTLTGQGTLAREIEQQMTEADLGGIDTILV
ECCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
STGGGGLIGGISAWFRDRIKVVSIETEGTNTLERSLREGPDLDVPASGIAAGSLGGPRLG
ECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
IDSYALIKAFVDEAIILPDTEVFTAAKRLWDGTRLIGEPGSAVALAALTSGAYVPEKDER
CCHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCC
VCVVLCGGNAEPDWFAT
EEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]