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The map label for this gene is glyS

Identifier: 89055222

GI number: 89055222

Start: 2743519

End: 2745738

Strand: Reverse

Name: glyS

Synonym: Jann_2731

Alternate gene names: 89055222

Gene position: 2745738-2743519 (Counterclockwise)

Preceding gene: 89055223

Following gene: 89055221

Centisome position: 63.59

GC content: 60.41

Gene sequence:

>2220_bases
ATGCCTGATCTTTTGCTGGAACTCTTTTCCGAGGAAATCCCCGCCCGGATGCAGGCGCGCGCGAGCGCTGATGTGAAGCG
TCTGGTGACCGATGGTTTGGTCGAGGCCGGGCTGACCTATGCCTCCGCCGGCAGTTTCGTCACCCCCCGCCGTCTGGTCC
TGACCGTGGAGGGTTTGACGGACAACTCGCCTACAACCACAGAAGACCGGCGTGGTCCCAAAGTTGGCGCGCCCGACAAG
GCCCTTGAAGGGTTTTTGCGTGGCGCAGGCGTCGCGCGTGAGGATCTGGAGGAGCGTGAGGACAAAAAAGGCACTTTCTA
CTACGCGAAGGTCACGTCCGAGGGCCGCGCGACGTCCGACATCATCGCCGACCTGATCCCGCAGATCGTGCGCAATTTCC
CGTGGCCCAAATCCATGCGGTGGGGCACGGGGTCTTTGCGTTGGGTGCGTCCATTGCAGTCCATCCTGTGCCTGTTGACC
CGCGAAGATGGCTCAGACGTGGTGAATTTCGACGTGGACGGCATCGCATCCGGCAACACAACCGAAGGCCACCGCTTCAT
GGCGCCTGGCCGGTTTAGCGTGTCGTCGTTCGATGATTACTCTGCCAAACTGCGCGCGGCCAAGGTGGTTCTGTCGCCGC
AGGACCGGTCTGACGCGATCTGGCACGACGCCACCAATATGGCCTTCGCGGCGGGGTTGGAGGTTGTTGAAGACGCGGGC
CTACTGACGGAGGTTGCGGGCCTCGTGGAATGGCCCGTTGTGTTGATGGGAGAGATCGCCGAGGGTTTCTTGGGCCTGCC
CGCGGAGGTCCTGCAAACCTCCATGCGCGAACACCAGAAGTTTTTCTCGGTCCGCAACCCGGCAACGGGCCGGATCGAAC
GGTTTATCACCGTGGCGAACCGGGAGACGGCGGACGAGGGCGCTACCATCCTCGCGGGGAATTCCAAGGTGCTGTCCGCG
CGTTTGGCGGACGGGAAATTCTTCTGGGACAACGACCTGCGCATCGCCCGTGATGGTATGGGTGCATGGCGCAAGGCATT
GGAAAACGTGACGTTCGAGCGGCGCTTGGGATCACAGGCCGAACGTGTCGAGCGTATCGCGACCCTCGCCCTAGAGATAG
CCCCCATGGTCGGCGCGGACCCGACGGCGGCGGAAACCGCTGCGCGGATCGCCAAGGCGGATCTGTCGTCCGAGATGGTC
TACGAGTTCCCCGAATTGCAAGGCACGATGGGCAAATACTACGCGTTGGAAGCGGGCCTCGACCCGGCGATTGCCGCCGT
GGCCGAAGACCACTACGCGCCTTTGGGCCCGACCGATAATGTTCCCACGGCGCCTTTGAGTATTGCGGTGGCATTGGCTG
ACAAACTTGACCTGCTGACAGGCTTCTGGGCGATTGATGAGAAGCCAACCGGCTCTAAAGATCCGTTTGCGTTGAGGCGT
GCGGCGCTGGGGGTTATCCGGATTGTTTTGGATGGTGGTTTGCGAGTGAGACTAGACCGGCTCTTCGATATGCAACTTGT
CGTGCACAAGGGGGCGATTATCGACCCAATAGACCGCGATGAAGTTGCTGAACTTGTCCGAGAAATCGCAAATCATGGGG
TATTTGGGGCGGCTTGGAAGGCAGTGAAAGAGTCGCGAGGCGGGCTCCCGTCATCCGGCTTTATTGAGATGAAAGAACAA
GTTCCTGACAAATCTGATGATCTCCTCGCCTTCTTCCACGACCGCCTCAAAGTTCATCTCAAGGCCGAGAACATCCGCCA
CGACGTCATTGACGCCGCCCTCTCCATGCCATCCAACGACGACCTCAGCCTCGTCGTGGCCCGTGCCCGCGCGCTGCAAG
CCTTCCTCGACACGCCCGACGGCGAAAACCTGATCCAGGGTTACAAACGCTCCGCCAACATCCTCGCCCAGGCCGAGGCC
AAGGACGGCGTTGAATACCGCTATGGCGCGGACGTGAAATATGCCGAAACCGATGAAGAACGCGCGCTCTTCACCGCTCT
GGACGTGGCGCGGACAGCCATTGCGCCCGCATTGGCCGAGGAGCGGTTTGAGGAAGCGATGACCGAAATGGCGAAACTCC
GCGCACCCATTGATGCGTTCTTTGAAGCTGTTCAAGTCAACGCCGACAATGATATTGTACGGCGCAACAGGCTGAATTTG
CTCAATGATATTGCTCAGACCTGTGGGGCCGTTGCGGACTTGACCAGGATCGAAGGCTGA

Upstream 100 bases:

>100_bases
AACGCAACGCATCGCGCGGCGTGGACCGCGTGGTGGCGATCTTTCCCGACGGGCGCGGCTACGCCTGGACGCAACTCAAC
GGCACTTTGGAGTAAGTCCC

Downstream 100 bases:

>100_bases
CGCCGCAGCGCAGCAACACCCCGCGCAAAACTGTCACATGAGTGTCCGGTTTACTTTACACTCATCCCTCGCGCTGTATC
TTTTGCCGGTATCAATTCGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MPDLLLELFSEEIPARMQARASADVKRLVTDGLVEAGLTYASAGSFVTPRRLVLTVEGLTDNSPTTTEDRRGPKVGAPDK
ALEGFLRGAGVAREDLEEREDKKGTFYYAKVTSEGRATSDIIADLIPQIVRNFPWPKSMRWGTGSLRWVRPLQSILCLLT
REDGSDVVNFDVDGIASGNTTEGHRFMAPGRFSVSSFDDYSAKLRAAKVVLSPQDRSDAIWHDATNMAFAAGLEVVEDAG
LLTEVAGLVEWPVVLMGEIAEGFLGLPAEVLQTSMREHQKFFSVRNPATGRIERFITVANRETADEGATILAGNSKVLSA
RLADGKFFWDNDLRIARDGMGAWRKALENVTFERRLGSQAERVERIATLALEIAPMVGADPTAAETAARIAKADLSSEMV
YEFPELQGTMGKYYALEAGLDPAIAAVAEDHYAPLGPTDNVPTAPLSIAVALADKLDLLTGFWAIDEKPTGSKDPFALRR
AALGVIRIVLDGGLRVRLDRLFDMQLVVHKGAIIDPIDRDEVAELVREIANHGVFGAAWKAVKESRGGLPSSGFIEMKEQ
VPDKSDDLLAFFHDRLKVHLKAENIRHDVIDAALSMPSNDDLSLVVARARALQAFLDTPDGENLIQGYKRSANILAQAEA
KDGVEYRYGADVKYAETDEERALFTALDVARTAIAPALAEERFEEAMTEMAKLRAPIDAFFEAVQVNADNDIVRRNRLNL
LNDIAQTCGAVADLTRIEG

Sequences:

>Translated_739_residues
MPDLLLELFSEEIPARMQARASADVKRLVTDGLVEAGLTYASAGSFVTPRRLVLTVEGLTDNSPTTTEDRRGPKVGAPDK
ALEGFLRGAGVAREDLEEREDKKGTFYYAKVTSEGRATSDIIADLIPQIVRNFPWPKSMRWGTGSLRWVRPLQSILCLLT
REDGSDVVNFDVDGIASGNTTEGHRFMAPGRFSVSSFDDYSAKLRAAKVVLSPQDRSDAIWHDATNMAFAAGLEVVEDAG
LLTEVAGLVEWPVVLMGEIAEGFLGLPAEVLQTSMREHQKFFSVRNPATGRIERFITVANRETADEGATILAGNSKVLSA
RLADGKFFWDNDLRIARDGMGAWRKALENVTFERRLGSQAERVERIATLALEIAPMVGADPTAAETAARIAKADLSSEMV
YEFPELQGTMGKYYALEAGLDPAIAAVAEDHYAPLGPTDNVPTAPLSIAVALADKLDLLTGFWAIDEKPTGSKDPFALRR
AALGVIRIVLDGGLRVRLDRLFDMQLVVHKGAIIDPIDRDEVAELVREIANHGVFGAAWKAVKESRGGLPSSGFIEMKEQ
VPDKSDDLLAFFHDRLKVHLKAENIRHDVIDAALSMPSNDDLSLVVARARALQAFLDTPDGENLIQGYKRSANILAQAEA
KDGVEYRYGADVKYAETDEERALFTALDVARTAIAPALAEERFEEAMTEMAKLRAPIDAFFEAVQVNADNDIVRRNRLNL
LNDIAQTCGAVADLTRIEG
>Mature_738_residues
PDLLLELFSEEIPARMQARASADVKRLVTDGLVEAGLTYASAGSFVTPRRLVLTVEGLTDNSPTTTEDRRGPKVGAPDKA
LEGFLRGAGVAREDLEEREDKKGTFYYAKVTSEGRATSDIIADLIPQIVRNFPWPKSMRWGTGSLRWVRPLQSILCLLTR
EDGSDVVNFDVDGIASGNTTEGHRFMAPGRFSVSSFDDYSAKLRAAKVVLSPQDRSDAIWHDATNMAFAAGLEVVEDAGL
LTEVAGLVEWPVVLMGEIAEGFLGLPAEVLQTSMREHQKFFSVRNPATGRIERFITVANRETADEGATILAGNSKVLSAR
LADGKFFWDNDLRIARDGMGAWRKALENVTFERRLGSQAERVERIATLALEIAPMVGADPTAAETAARIAKADLSSEMVY
EFPELQGTMGKYYALEAGLDPAIAAVAEDHYAPLGPTDNVPTAPLSIAVALADKLDLLTGFWAIDEKPTGSKDPFALRRA
ALGVIRIVLDGGLRVRLDRLFDMQLVVHKGAIIDPIDRDEVAELVREIANHGVFGAAWKAVKESRGGLPSSGFIEMKEQV
PDKSDDLLAFFHDRLKVHLKAENIRHDVIDAALSMPSNDDLSLVVARARALQAFLDTPDGENLIQGYKRSANILAQAEAK
DGVEYRYGADVKYAETDEERALFTALDVARTAIAPALAEERFEEAMTEMAKLRAPIDAFFEAVQVNADNDIVRRNRLNLL
NDIAQTCGAVADLTRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=747, Percent_Identity=31.9946452476573, Blast_Score=335, Evalue=4e-93,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80724; Mature: 80593

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELFSEEIPARMQARASADVKRLVTDGLVEAGLTYASAGSFVTPRRLVLTVEGLT
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCC
DNSPTTTEDRRGPKVGAPDKALEGFLRGAGVAREDLEEREDKKGTFYYAKVTSEGRATSD
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHH
IIADLIPQIVRNFPWPKSMRWGTGSLRWVRPLQSILCLLTREDGSDVVNFDVDGIASGNT
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
TEGHRFMAPGRFSVSSFDDYSAKLRAAKVVLSPQDRSDAIWHDATNMAFAAGLEVVEDAG
CCCCEEECCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHCH
LLTEVAGLVEWPVVLMGEIAEGFLGLPAEVLQTSMREHQKFFSVRNPATGRIERFITVAN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEECC
RETADEGATILAGNSKVLSARLADGKFFWDNDLRIARDGMGAWRKALENVTFERRLGSQA
CCCCCCCCEEEECCCCEEEEEECCCEEEECCCCEEECCCHHHHHHHHHCCHHHHHHCHHH
ERVERIATLALEIAPMVGADPTAAETAARIAKADLSSEMVYEFPELQGTMGKYYALEAGL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHCHHHHCCCCCEEEEECCC
DPAIAAVAEDHYAPLGPTDNVPTAPLSIAVALADKLDLLTGFWAIDEKPTGSKDPFALRR
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHH
AALGVIRIVLDGGLRVRLDRLFDMQLVVHKGAIIDPIDRDEVAELVREIANHGVFGAAWK
HHHHHHHHEECCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
AVKESRGGLPSSGFIEMKEQVPDKSDDLLAFFHDRLKVHLKAENIRHDVIDAALSMPSND
HHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCC
DLSLVVARARALQAFLDTPDGENLIQGYKRSANILAQAEAKDGVEYRYGADVKYAETDEE
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHEEECCCCCCCCEEECCCCEECCCCCH
RALFTALDVARTAIAPALAEERFEEAMTEMAKLRAPIDAFFEAVQVNADNDIVRRNRLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHH
LNDIAQTCGAVADLTRIEG
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDLLLELFSEEIPARMQARASADVKRLVTDGLVEAGLTYASAGSFVTPRRLVLTVEGLT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCC
DNSPTTTEDRRGPKVGAPDKALEGFLRGAGVAREDLEEREDKKGTFYYAKVTSEGRATSD
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHH
IIADLIPQIVRNFPWPKSMRWGTGSLRWVRPLQSILCLLTREDGSDVVNFDVDGIASGNT
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
TEGHRFMAPGRFSVSSFDDYSAKLRAAKVVLSPQDRSDAIWHDATNMAFAAGLEVVEDAG
CCCCEEECCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHCH
LLTEVAGLVEWPVVLMGEIAEGFLGLPAEVLQTSMREHQKFFSVRNPATGRIERFITVAN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEECC
RETADEGATILAGNSKVLSARLADGKFFWDNDLRIARDGMGAWRKALENVTFERRLGSQA
CCCCCCCCEEEECCCCEEEEEECCCEEEECCCCEEECCCHHHHHHHHHCCHHHHHHCHHH
ERVERIATLALEIAPMVGADPTAAETAARIAKADLSSEMVYEFPELQGTMGKYYALEAGL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHCHHHHCCCCCEEEEECCC
DPAIAAVAEDHYAPLGPTDNVPTAPLSIAVALADKLDLLTGFWAIDEKPTGSKDPFALRR
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHH
AALGVIRIVLDGGLRVRLDRLFDMQLVVHKGAIIDPIDRDEVAELVREIANHGVFGAAWK
HHHHHHHHEECCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
AVKESRGGLPSSGFIEMKEQVPDKSDDLLAFFHDRLKVHLKAENIRHDVIDAALSMPSND
HHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCCCC
DLSLVVARARALQAFLDTPDGENLIQGYKRSANILAQAEAKDGVEYRYGADVKYAETDEE
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHEEECCCCCCCCEEECCCCEECCCCCH
RALFTALDVARTAIAPALAEERFEEAMTEMAKLRAPIDAFFEAVQVNADNDIVRRNRLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHH
LNDIAQTCGAVADLTRIEG
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA