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The map label for this gene is gltD [H]

Identifier: 89055204

GI number: 89055204

Start: 2722115

End: 2723455

Strand: Reverse

Name: gltD [H]

Synonym: Jann_2713

Alternate gene names: 89055204

Gene position: 2723455-2722115 (Counterclockwise)

Preceding gene: 89055209

Following gene: 89055203

Centisome position: 63.07

GC content: 64.65

Gene sequence:

>1341_bases
ATGCGCAACACTCCTTTCACCCCCGGCATCGCGCCCGCGCGCCTTGAGGCGACCGAGCTGGCGCAGAATTTCAGCGATCT
CCACGCGCCGCTCGATGGGCACGAGGCGCTGGTTGCCGCTGATCGATGCTACTTCTGCCATGACGCGCCCTGCATCACGG
CTTGCCCGACCTCCATCGATATCCCCCTGTTCATCCGCCAGATCGCGACCGGCACGCCAGAGGCTGCGGCGCGCACGATC
TTGGATCAGAACATTCTGGGCGGCATGTGCGCCCGCGTGTGTCCGACAGAGACACTCTGTGAGGAGGCCTGCGTGCGCGA
AGCCGCCGAAGGCAAGCCGGTGCTGATCGGTCAGCTCCAACGCTATGCCACCGACATTGTGATGGACGCGGGCGTACATC
CGTTCCAGCGTGCCGAGGTCACCGGCAAGAAGGTGGCTGTGGTCGGGGCCGGGCCTGCGGGGCTGGCCTGCGCCCATCGC
CTGGCGATGCATGGCCACAGCGTCGATATTTTCGACAAACGCGCCAAGGGTGGGGGGCTAAATGAATTCGGCATCGCCGC
CTACAAGAGCACCGATGATTTCGCCCAGGCCGAGGTGGATTGGCTGATGCAGATCGGCGGCATTCGTATGCTGACCGACC
GTGCCCTGGGGCAGGACCTGACGCTGGCGGAATTGCAGGAGGATTACGACGCGGTGTTCCTTGGTATGGGTCTTGCGGGC
GTGAATGCGCTTCGCGCCGAGGGTGAGGCACGCGATGGTGTCGGCGATGCCGTTGAATTCATTGCAGATCTGCGCCAGGC
CGATGATCTGGGCGTCCTGCCCGTTGGTCGCAATGTGGTGGTGATCGGCGGCGGCATGACGGCGATTGATGCGGCGGTGC
AGTCGAAACTTCTGGGCGCGGAAAGCGTGACGCTCGTTTATCGCAGGGGGCGGGAGGCGATGCCTGCCAGCCGGTATGAA
CAGGATCTCGCGGCCTCCAAAGGTGTGCGGATGATCTTCAACGCGATGCCGGTTGAGGTGCTTGGCAACGGCGCGGCAAC
GGCGCTTCGGTGCGAATATACCCGCGCGGAAGGCCGCAGCCTGACGGGCACCGGAGAGACCTTCGATATCCCCGCCGATC
AGGTCCTCAAGGCCATTGGTCAAACGCTGGACGGAGCACCCGGGGCGGTGGCGCTGGACGGTGGCAAGATTGCCGTCACC
GGGGCCGGACGCACCAGCGTGGATGGCGTTTGGGCCGGTGGCGATTGCGCGTCGGGCGGGGAAGACCTGACCGTGACCGC
CGTGGCGGAAGGCCGCGACGCGGCGGAGGATATTCATATCGTATTGACAGGTAAGGAATAA

Upstream 100 bases:

>100_bases
GGCAAAAATCTGCCTCGCTCCGAAATTTTACCATTTGATAAATTTTTGAAGCCGTGTCAGCGTGATTCCCGACCAAGCCA
TCACGTAATAGCGAGGCCCC

Downstream 100 bases:

>100_bases
ATCATGGCTGACCTGACCTCCAACTTCATCGGCATCACTTCGCCCAACCCCTTCTGGCTCGCCTCCGCGCCGCCGACGGA
CAAGGAATACAACGTCCGCC

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MRNTPFTPGIAPARLEATELAQNFSDLHAPLDGHEALVAADRCYFCHDAPCITACPTSIDIPLFIRQIATGTPEAAARTI
LDQNILGGMCARVCPTETLCEEACVREAAEGKPVLIGQLQRYATDIVMDAGVHPFQRAEVTGKKVAVVGAGPAGLACAHR
LAMHGHSVDIFDKRAKGGGLNEFGIAAYKSTDDFAQAEVDWLMQIGGIRMLTDRALGQDLTLAELQEDYDAVFLGMGLAG
VNALRAEGEARDGVGDAVEFIADLRQADDLGVLPVGRNVVVIGGGMTAIDAAVQSKLLGAESVTLVYRRGREAMPASRYE
QDLAASKGVRMIFNAMPVEVLGNGAATALRCEYTRAEGRSLTGTGETFDIPADQVLKAIGQTLDGAPGAVALDGGKIAVT
GAGRTSVDGVWAGGDCASGGEDLTVTAVAEGRDAAEDIHIVLTGKE

Sequences:

>Translated_446_residues
MRNTPFTPGIAPARLEATELAQNFSDLHAPLDGHEALVAADRCYFCHDAPCITACPTSIDIPLFIRQIATGTPEAAARTI
LDQNILGGMCARVCPTETLCEEACVREAAEGKPVLIGQLQRYATDIVMDAGVHPFQRAEVTGKKVAVVGAGPAGLACAHR
LAMHGHSVDIFDKRAKGGGLNEFGIAAYKSTDDFAQAEVDWLMQIGGIRMLTDRALGQDLTLAELQEDYDAVFLGMGLAG
VNALRAEGEARDGVGDAVEFIADLRQADDLGVLPVGRNVVVIGGGMTAIDAAVQSKLLGAESVTLVYRRGREAMPASRYE
QDLAASKGVRMIFNAMPVEVLGNGAATALRCEYTRAEGRSLTGTGETFDIPADQVLKAIGQTLDGAPGAVALDGGKIAVT
GAGRTSVDGVWAGGDCASGGEDLTVTAVAEGRDAAEDIHIVLTGKE
>Mature_446_residues
MRNTPFTPGIAPARLEATELAQNFSDLHAPLDGHEALVAADRCYFCHDAPCITACPTSIDIPLFIRQIATGTPEAAARTI
LDQNILGGMCARVCPTETLCEEACVREAAEGKPVLIGQLQRYATDIVMDAGVHPFQRAEVTGKKVAVVGAGPAGLACAHR
LAMHGHSVDIFDKRAKGGGLNEFGIAAYKSTDDFAQAEVDWLMQIGGIRMLTDRALGQDLTLAELQEDYDAVFLGMGLAG
VNALRAEGEARDGVGDAVEFIADLRQADDLGVLPVGRNVVVIGGGMTAIDAAVQSKLLGAESVTLVYRRGREAMPASRYE
QDLAASKGVRMIFNAMPVEVLGNGAATALRCEYTRAEGRSLTGTGETFDIPADQVLKAIGQTLDGAPGAVALDGGKIAVT
GAGRTSVDGVWAGGDCASGGEDLTVTAVAEGRDAAEDIHIVLTGKE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=31.9383259911894, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI237757300, Length=115, Percent_Identity=41.7391304347826, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789606, Length=458, Percent_Identity=36.2445414847162, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87082180, Length=462, Percent_Identity=34.6320346320346, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788468, Length=424, Percent_Identity=34.9056603773585, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1788811, Length=463, Percent_Identity=35.2051835853132, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789243, Length=405, Percent_Identity=29.1358024691358, Blast_Score=115, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71984108, Length=455, Percent_Identity=31.4285714285714, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=452, Percent_Identity=27.4336283185841, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320030, Length=459, Percent_Identity=27.4509803921569, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640763, Length=457, Percent_Identity=34.5733041575492, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI18858217, Length=457, Percent_Identity=34.5733041575492, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24665547, Length=476, Percent_Identity=30.0420168067227, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24665543, Length=476, Percent_Identity=30.0420168067227, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28574881, Length=477, Percent_Identity=30.398322851153, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24665539, Length=477, Percent_Identity=30.398322851153, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 46518; Mature: 46518

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTPFTPGIAPARLEATELAQNFSDLHAPLDGHEALVAADRCYFCHDAPCITACPTSID
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEECCCCCEEECCCCCC
IPLFIRQIATGTPEAAARTILDQNILGGMCARVCPTETLCEEACVREAAEGKPVLIGQLQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHH
RYATDIVMDAGVHPFQRAEVTGKKVAVVGAGPAGLACAHRLAMHGHSVDIFDKRAKGGGL
HHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCC
NEFGIAAYKSTDDFAQAEVDWLMQIGGIRMLTDRALGQDLTLAELQEDYDAVFLGMGLAG
CCCCEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHCCCCCHHHHHHHHHHEEEECCCHHH
VNALRAEGEARDGVGDAVEFIADLRQADDLGVLPVGRNVVVIGGGMTAIDAAVQSKLLGA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHCCC
ESVTLVYRRGREAMPASRYEQDLAASKGVRMIFNAMPVEVLGNGAATALRCEYTRAEGRS
CCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCEEECCCCCEEEEEEEECCCCCE
LTGTGETFDIPADQVLKAIGQTLDGAPGAVALDGGKIAVTGAGRTSVDGVWAGGDCASGG
ECCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCEEEECCCCCCCC
EDLTVTAVAEGRDAAEDIHIVLTGKE
CCEEEEEEECCCCCCCCEEEEEECCC
>Mature Secondary Structure
MRNTPFTPGIAPARLEATELAQNFSDLHAPLDGHEALVAADRCYFCHDAPCITACPTSID
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEECCCCCEEECCCCCC
IPLFIRQIATGTPEAAARTILDQNILGGMCARVCPTETLCEEACVREAAEGKPVLIGQLQ
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHH
RYATDIVMDAGVHPFQRAEVTGKKVAVVGAGPAGLACAHRLAMHGHSVDIFDKRAKGGGL
HHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCC
NEFGIAAYKSTDDFAQAEVDWLMQIGGIRMLTDRALGQDLTLAELQEDYDAVFLGMGLAG
CCCCEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHCCCCCHHHHHHHHHHEEEECCCHHH
VNALRAEGEARDGVGDAVEFIADLRQADDLGVLPVGRNVVVIGGGMTAIDAAVQSKLLGA
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHCCC
ESVTLVYRRGREAMPASRYEQDLAASKGVRMIFNAMPVEVLGNGAATALRCEYTRAEGRS
CCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCEEECCCCCEEEEEEEECCCCCE
LTGTGETFDIPADQVLKAIGQTLDGAPGAVALDGGKIAVTGAGRTSVDGVWAGGDCASGG
ECCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCEEEECCCCCCCC
EDLTVTAVAEGRDAAEDIHIVLTGKE
CCEEEEEEECCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]