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The map label for this gene is rlmI [H]

Identifier: 89055180

GI number: 89055180

Start: 2698798

End: 2699994

Strand: Reverse

Name: rlmI [H]

Synonym: Jann_2689

Alternate gene names: 89055180

Gene position: 2699994-2698798 (Counterclockwise)

Preceding gene: 89055181

Following gene: 89055179

Centisome position: 62.53

GC content: 66.58

Gene sequence:

>1197_bases
ATGACAGCGCCCTCTTTTCCCGTCCTTCGCCTGAAACCCAAAGCCGATGCGCGCCGCATCCGCCATGGCCATCCCTGGGC
CTGGGCCGATGATCTCGTGCTGGATCGCCGTTCCAAGGCCGTGCCTGCGGGCGCGTTGGCCATTTTGGAGGATGCGGAGC
GTCAGCGCATCGGCGTGGGGGTGGCAACGGTCGGCGCGCGCATTGGCCTACGAGTGCTGGACCGCGCGCCTGATGCGGAG
ATCAACGTGAATTGGCTGCGCACGAGGATTACACGCGCCACAGCCCTGCGAGATGCCCTCTACGACGCGCCGTTCTATCG
CCTGATCCATGCCGAAGGGGACGGTTTGCCCGGCCTGATCGTCGATCGGTTCGGCGACACGCTGGTGATGCAGCCCAATG
CGATCTGGCTGGAGGAGCGGCTGGACGATCTGCAAACGCTGCTGCTGGAGGCCACAGGGGCCTCGACGCTGATCAAGAAC
GGCACGTCGCGCGCCCGTGCATTGGAAGATTTGCCGGAGGAGATGGTGACCCTGACTGGCACCGCGCCCGCCGGTCCGCT
GGAGGTGCCAATGACCGGTGCGCTCTATATGGCGGATGTGATGGGCGGTCAGAAAACCGGCCTGTTCTACGACCAACGGC
CCAATCACGCATTCGTTGCGGGGCTCGCAACGGGCAAACGTGTGCTGGACGTGTTCACCCATGTGGGCGGGTTTGCTTTG
GCGGCGCTTGCGGCGGGGGCCGACACGGCCCTGGCCGTCGACGCTTCAGACCCGGCGCTGCAATTGGCGGGGCAGGGGGC
GAAAGCCATGGGGTGCGCCGAGCGGTTCGCAACGCGCCAAGGCGATGCCTTCGCGGTGATGGAGGCGTTGGCCGCAGAGG
GGGAGACCTTCGACATCGTCATCGCCGACCCGCCCGCCTTCGCGCCGTCCAAACAGGCGCTGGACAAGGGGTTGCGGGCT
TATGAGCGGGTCGCGCGGCTGGCCACGTCGCTGGTGGCCGAGGGTGGCACGCTGATGCTGTGCTCGTGTTCTCACGCGGC
GGATCTGGGCAAATTCCGCGCCTCCTGCCTGCGCGGCATCGGACGTGCGGGACGCGAGCCGCGCATCATCAATACCGGGT
TTGCGGGGCCGGATCATCCGGTCCATCCCAGCCTGAGCGACACGGGCTACCTGAAGGCCCTCGCGTTCCACTTATGA

Upstream 100 bases:

>100_bases
TTGGGTGACCGAGGAATTCATTGGCCCGGCCGATGCACCGCTGATTGTCGCCGCCAACTGAGGCTTTCGCACCCTTGACG
AACCCGGTAGAGGGGGCGCC

Downstream 100 bases:

>100_bases
TCGCGGTCATCGACGCCTGCGCGCTGTATCCGACCGTGCTGCGTCAGATCCTGTTGGGTTGCGCGGAGCAGGATATGTTC
ACCCCGATCTGGTCTGACCG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTAPSFPVLRLKPKADARRIRHGHPWAWADDLVLDRRSKAVPAGALAILEDAERQRIGVGVATVGARIGLRVLDRAPDAE
INVNWLRTRITRATALRDALYDAPFYRLIHAEGDGLPGLIVDRFGDTLVMQPNAIWLEERLDDLQTLLLEATGASTLIKN
GTSRARALEDLPEEMVTLTGTAPAGPLEVPMTGALYMADVMGGQKTGLFYDQRPNHAFVAGLATGKRVLDVFTHVGGFAL
AALAAGADTALAVDASDPALQLAGQGAKAMGCAERFATRQGDAFAVMEALAAEGETFDIVIADPPAFAPSKQALDKGLRA
YERVARLATSLVAEGGTLMLCSCSHAADLGKFRASCLRGIGRAGREPRIINTGFAGPDHPVHPSLSDTGYLKALAFHL

Sequences:

>Translated_398_residues
MTAPSFPVLRLKPKADARRIRHGHPWAWADDLVLDRRSKAVPAGALAILEDAERQRIGVGVATVGARIGLRVLDRAPDAE
INVNWLRTRITRATALRDALYDAPFYRLIHAEGDGLPGLIVDRFGDTLVMQPNAIWLEERLDDLQTLLLEATGASTLIKN
GTSRARALEDLPEEMVTLTGTAPAGPLEVPMTGALYMADVMGGQKTGLFYDQRPNHAFVAGLATGKRVLDVFTHVGGFAL
AALAAGADTALAVDASDPALQLAGQGAKAMGCAERFATRQGDAFAVMEALAAEGETFDIVIADPPAFAPSKQALDKGLRA
YERVARLATSLVAEGGTLMLCSCSHAADLGKFRASCLRGIGRAGREPRIINTGFAGPDHPVHPSLSDTGYLKALAFHL
>Mature_397_residues
TAPSFPVLRLKPKADARRIRHGHPWAWADDLVLDRRSKAVPAGALAILEDAERQRIGVGVATVGARIGLRVLDRAPDAEI
NVNWLRTRITRATALRDALYDAPFYRLIHAEGDGLPGLIVDRFGDTLVMQPNAIWLEERLDDLQTLLLEATGASTLIKNG
TSRARALEDLPEEMVTLTGTAPAGPLEVPMTGALYMADVMGGQKTGLFYDQRPNHAFVAGLATGKRVLDVFTHVGGFALA
ALAAGADTALAVDASDPALQLAGQGAKAMGCAERFATRQGDAFAVMEALAAEGETFDIVIADPPAFAPSKQALDKGLRAY
ERVARLATSLVAEGGTLMLCSCSHAADLGKFRASCLRGIGRAGREPRIINTGFAGPDHPVHPSLSDTGYLKALAFHL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=380, Percent_Identity=29.2105263157895, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42299; Mature: 42168

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSFPVLRLKPKADARRIRHGHPWAWADDLVLDRRSKAVPAGALAILEDAERQRIGVG
CCCCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCH
VATVGARIGLRVLDRAPDAEINVNWLRTRITRATALRDALYDAPFYRLIHAEGDGLPGLI
HHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHH
VDRFGDTLVMQPNAIWLEERLDDLQTLLLEATGASTLIKNGTSRARALEDLPEEMVTLTG
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHEEEEC
TAPAGPLEVPMTGALYMADVMGGQKTGLFYDQRPNHAFVAGLATGKRVLDVFTHVGGFAL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHH
AALAAGADTALAVDASDPALQLAGQGAKAMGCAERFATRQGDAFAVMEALAAEGETFDIV
HHHHCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
IADPPAFAPSKQALDKGLRAYERVARLATSLVAEGGTLMLCSCSHAADLGKFRASCLRGI
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHH
GRAGREPRIINTGFAGPDHPVHPSLSDTGYLKALAFHL
HHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TAPSFPVLRLKPKADARRIRHGHPWAWADDLVLDRRSKAVPAGALAILEDAERQRIGVG
CCCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCH
VATVGARIGLRVLDRAPDAEINVNWLRTRITRATALRDALYDAPFYRLIHAEGDGLPGLI
HHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHH
VDRFGDTLVMQPNAIWLEERLDDLQTLLLEATGASTLIKNGTSRARALEDLPEEMVTLTG
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHEEEEC
TAPAGPLEVPMTGALYMADVMGGQKTGLFYDQRPNHAFVAGLATGKRVLDVFTHVGGFAL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHH
AALAAGADTALAVDASDPALQLAGQGAKAMGCAERFATRQGDAFAVMEALAAEGETFDIV
HHHHCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
IADPPAFAPSKQALDKGLRAYERVARLATSLVAEGGTLMLCSCSHAADLGKFRASCLRGI
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHH
GRAGREPRIINTGFAGPDHPVHPSLSDTGYLKALAFHL
HHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA