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The map label for this gene is pucL [H]

Identifier: 89055099

GI number: 89055099

Start: 2623884

End: 2625296

Strand: Reverse

Name: pucL [H]

Synonym: Jann_2608

Alternate gene names: 89055099

Gene position: 2625296-2623884 (Counterclockwise)

Preceding gene: 89055101

Following gene: 89055098

Centisome position: 60.8

GC content: 62.49

Gene sequence:

>1413_bases
GTGAACCGCTACCCACGCGATATGTCCGGATACGGGGCCAACCCACCCGCCGCCAATTGGCCGGGAGGGGCAAAGATCGC
TGTCAACTTTGTGCTTAATTATGAGGAGGGTGGCGAGAACAACATCCTCCACGGCGATGGGGCGTCCGAGGCGTTTTTGT
CGGAGATCACCGGGGCCGCCCCCTGGACGGGGCAGCGCCATTGGAACATGGAATCGCTATACGAATATGGCGCGCGCGCC
GGGTTCTGGCGCGTCCATCGCATCCTGAACGACGTGCCTGTGACGGTCTACGGTGTGGCGACAGCGCTGGCCCGCGCGCC
GGAACAGGTGGCCGCAATGACGGCTGCGGGTTGGGAAATTGCCAGCCACGGCCTCAAGTGGGTGGAGCATAAGGACATGC
CCGCGGAGGAGGAACGCGCCCAGATCGTTGAAGCGATCCGCCTGCACACCGAAGTGACGGGCCAGCCGCCGCGCGGTTGG
TACACGGGGCGCTGCTCGATGAACACCGTTGATCTGGCCGCGGAAGAGGGCGATCTGGCCTATATCTCAGACGATTACGC
CGATGATCTCCCCTATTGGGTGACGGCGGGCGGCCAGGATCAGCTGATCTTGCCCTACACGATGGATTGCAACGACATGC
GCTTTGCGATCCAGGCGGGCTTCACCACAGGTGCTGATTTTGAGGCGATGCTGCGCGATGCGTTCGACACGCTTTATGCA
GAAGGTCAGGCAGGTGCGCCCAAGATGATGACCATCGGGCTGCATTGTCGCATCATCGGGCGCCCAAGTCGGGCGGCCGC
GTTGAAACGTGTGCTGGAGTATATGCAAGGTCACGATGATGTCTGGTTCGCGACCCGCGAGGACATTGCGGACCATTGGG
CGGCAGAGCATCCCCCTCGCCACCGCGCGCGCCCTTCGCAGATGGACCGGGACGCCTTCGTGGAGGCATTTGGCGGCATC
TTCGAGCATTCCCCATGGATCGCGGAGGGGGCCCACGCGCTGGAACTTGGACCCATGCATGACAGTGCGGTTGGCGTCCA
CTCGGCCCTTGCACGGGTGTTCCGCTCGGCGTCCGAGGGGCAACGTTTGGATGTCCTGAAGGCGCATCCCGATCTGGCGG
GCAAGCTGGCGGCTGCGGGCAAACTGACAGCAGAATCGACCGCCGAGCAAGCGGGCGCGGGGCTGGATCTGCTGACCGAT
GAGGAGCGCGCAACCTTCCAAACCCTCAACGCGCAATACGTGGATCGCCACGGCTTTCCTTTCATCATCGCCGTCAAAGA
CAACACCAAAGCGTCTATTCTTGAGGCATTCCACACCCGCATCGCCAACCCACGCGACATCGAATTCACCGAGGCCTGCA
AACAGGTCGAACGGATTGCCGAGTTGCGGCTGTTGGAGAAACTGGGCCAATGA

Upstream 100 bases:

>100_bases
GGTGCAAAGCAAGGTGGACCGAAGGCTCCTTCAAGAATAATTTGAAAAATGTTTCCATTTTCTTGGAATAGAAGTCGTTA
ACATCGAAAGGATCTCGCTC

Downstream 100 bases:

>100_bases
TCCCGCTGACCGCACAGCCCCTGACATCCACGGCCTTCGCCCCCTTCGGCGACGTGCTGGAGGCCGAGGGTTCGCCTGAC
AAGATCATTAACGAGGGGCG

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNRYPRDMSGYGANPPAANWPGGAKIAVNFVLNYEEGGENNILHGDGASEAFLSEITGAAPWTGQRHWNMESLYEYGARA
GFWRVHRILNDVPVTVYGVATALARAPEQVAAMTAAGWEIASHGLKWVEHKDMPAEEERAQIVEAIRLHTEVTGQPPRGW
YTGRCSMNTVDLAAEEGDLAYISDDYADDLPYWVTAGGQDQLILPYTMDCNDMRFAIQAGFTTGADFEAMLRDAFDTLYA
EGQAGAPKMMTIGLHCRIIGRPSRAAALKRVLEYMQGHDDVWFATREDIADHWAAEHPPRHRARPSQMDRDAFVEAFGGI
FEHSPWIAEGAHALELGPMHDSAVGVHSALARVFRSASEGQRLDVLKAHPDLAGKLAAAGKLTAESTAEQAGAGLDLLTD
EERATFQTLNAQYVDRHGFPFIIAVKDNTKASILEAFHTRIANPRDIEFTEACKQVERIAELRLLEKLGQ

Sequences:

>Translated_470_residues
MNRYPRDMSGYGANPPAANWPGGAKIAVNFVLNYEEGGENNILHGDGASEAFLSEITGAAPWTGQRHWNMESLYEYGARA
GFWRVHRILNDVPVTVYGVATALARAPEQVAAMTAAGWEIASHGLKWVEHKDMPAEEERAQIVEAIRLHTEVTGQPPRGW
YTGRCSMNTVDLAAEEGDLAYISDDYADDLPYWVTAGGQDQLILPYTMDCNDMRFAIQAGFTTGADFEAMLRDAFDTLYA
EGQAGAPKMMTIGLHCRIIGRPSRAAALKRVLEYMQGHDDVWFATREDIADHWAAEHPPRHRARPSQMDRDAFVEAFGGI
FEHSPWIAEGAHALELGPMHDSAVGVHSALARVFRSASEGQRLDVLKAHPDLAGKLAAAGKLTAESTAEQAGAGLDLLTD
EERATFQTLNAQYVDRHGFPFIIAVKDNTKASILEAFHTRIANPRDIEFTEACKQVERIAELRLLEKLGQ
>Mature_470_residues
MNRYPRDMSGYGANPPAANWPGGAKIAVNFVLNYEEGGENNILHGDGASEAFLSEITGAAPWTGQRHWNMESLYEYGARA
GFWRVHRILNDVPVTVYGVATALARAPEQVAAMTAAGWEIASHGLKWVEHKDMPAEEERAQIVEAIRLHTEVTGQPPRGW
YTGRCSMNTVDLAAEEGDLAYISDDYADDLPYWVTAGGQDQLILPYTMDCNDMRFAIQAGFTTGADFEAMLRDAFDTLYA
EGQAGAPKMMTIGLHCRIIGRPSRAAALKRVLEYMQGHDDVWFATREDIADHWAAEHPPRHRARPSQMDRDAFVEAFGGI
FEHSPWIAEGAHALELGPMHDSAVGVHSALARVFRSASEGQRLDVLKAHPDLAGKLAAAGKLTAESTAEQAGAGLDLLTD
EERATFQTLNAQYVDRHGFPFIIAVKDNTKASILEAFHTRIANPRDIEFTEACKQVERIAELRLLEKLGQ

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=164, Percent_Identity=34.7560975609756, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 51773; Mature: 51773

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYPRDMSGYGANPPAANWPGGAKIAVNFVLNYEEGGENNILHGDGASEAFLSEITGAA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCHHHHHHHHCCCC
PWTGQRHWNMESLYEYGARAGFWRVHRILNDVPVTVYGVATALARAPEQVAAMTAAGWEI
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCHHH
ASHGLKWVEHKDMPAEEERAQIVEAIRLHTEVTGQPPRGWYTGRCSMNTVDLAAEEGDLA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCCEE
YISDDYADDLPYWVTAGGQDQLILPYTMDCNDMRFAIQAGFTTGADFEAMLRDAFDTLYA
EEECCCCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHC
EGQAGAPKMMTIGLHCRIIGRPSRAAALKRVLEYMQGHDDVWFATREDIADHWAAEHPPR
CCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCC
HRARPSQMDRDAFVEAFGGIFEHSPWIAEGAHALELGPMHDSAVGVHSALARVFRSASEG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
QRLDVLKAHPDLAGKLAAAGKLTAESTAEQAGAGLDLLTDEERATFQTLNAQYVDRHGFP
CEEEEEECCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHCHHHHHCCCCE
FIIAVKDNTKASILEAFHTRIANPRDIEFTEACKQVERIAELRLLEKLGQ
EEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRYPRDMSGYGANPPAANWPGGAKIAVNFVLNYEEGGENNILHGDGASEAFLSEITGAA
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCHHHHHHHHCCCC
PWTGQRHWNMESLYEYGARAGFWRVHRILNDVPVTVYGVATALARAPEQVAAMTAAGWEI
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCHHH
ASHGLKWVEHKDMPAEEERAQIVEAIRLHTEVTGQPPRGWYTGRCSMNTVDLAAEEGDLA
HHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCCEE
YISDDYADDLPYWVTAGGQDQLILPYTMDCNDMRFAIQAGFTTGADFEAMLRDAFDTLYA
EEECCCCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHC
EGQAGAPKMMTIGLHCRIIGRPSRAAALKRVLEYMQGHDDVWFATREDIADHWAAEHPPR
CCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCC
HRARPSQMDRDAFVEAFGGIFEHSPWIAEGAHALELGPMHDSAVGVHSALARVFRSASEG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
QRLDVLKAHPDLAGKLAAAGKLTAESTAEQAGAGLDLLTDEERATFQTLNAQYVDRHGFP
CEEEEEECCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHCHHHHHCCCCE
FIIAVKDNTKASILEAFHTRIANPRDIEFTEACKQVERIAELRLLEKLGQ
EEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]