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The map label for this gene is dmlR [H]

Identifier: 89055101

GI number: 89055101

Start: 2625788

End: 2626666

Strand: Reverse

Name: dmlR [H]

Synonym: Jann_2610

Alternate gene names: 89055101

Gene position: 2626666-2625788 (Counterclockwise)

Preceding gene: 89055103

Following gene: 89055099

Centisome position: 60.83

GC content: 61.66

Gene sequence:

>879_bases
ATGTCTGCGGCGGGGCGCGATTTGCGTATTTCGCCAGCTGTCACGTCGTCTCGGATTTCGCAGCTGGAGGAGCATCTGAG
CGTGCGGCTGTTCCAGCGCACCACGCGCAGTCTGACGCCGACGGAACATGGCAAGGCCTTCTATTCCGGCGCGAAGGAAA
TCCTTGAATCGGTTGAGAATGCCGAAGCGCAAGTCATTGATATCACGGAGAACCCAAAGGGGTCACTCTATGTGGCTGCC
CCTTTGGGTGTGGGACGTCGCCTGATTGCCCCGGAGGTGCCGGGGTTCCTTGACCTTTATCCGGAGGTTCGCGTGCGCCT
GCGTCTGACCGACCGGAAAGTGGATCTGACGACCGAAGGCCTCGACCTCGCGTTTTTTCTGGGGGAGCCTGAGGACAGCA
CGCTTCGTATCCGCAAGATCGCGGACGTGGAACGGGTTCTGTGCGCCGCGCCAGACTACGTTGCGGCCCGCGGTATGCCT
GCGGGCGGTGCGGAGCTGACGTCTGGGGCGCATGAATGCCTCAACCTGCGATTTCCCGGCGCGACGGAATTCCAGTGGCA
CCTGAAGACACCGGACGGACCCAAGCGCTTCCGCATCCAGGGGCGCTACGAATCCGATGATGGTGATGTGCTGAATGACT
GGGCGTTGGCGGGTCAAGGCATTGTGATGAAACCGATTTTTGAGGTGGCCGAGCATCTGAAGGCGGGCCGGTTGGTGGCG
GTGGCGCAGGAAACGCCGCCGATGCCGATCCAGATGGCGTGCCTGTTCACCCATCGCCGGATGCAGGATCCGAAGACGCG
GCTCTTCATGGAATTCGTGATCAACCGCATTGGCGATCTGGTGCGGCAGGCCGAAAAGCTGGGTCAGCTGCCGCGATAG

Upstream 100 bases:

>100_bases
CTGCTATTGCAGGCAAAAGCCAAGCTGTATCAAAAAAGCCGGAACAATGTCCTATCTCGATAACATCCGAACCTTCGTGC
GTGTCTATGAATTGGGCTCC

Downstream 100 bases:

>100_bases
GTGGAATATCCGAAGGGCGAAAGCAGAAGCGGCACATGGTAATGGGCGTCTGCGTCGGACATGCCGAAGCGGATCGGGAT
TTGGTCCAGGAACAGGATCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSAAGRDLRISPAVTSSRISQLEEHLSVRLFQRTTRSLTPTEHGKAFYSGAKEILESVENAEAQVIDITENPKGSLYVAA
PLGVGRRLIAPEVPGFLDLYPEVRVRLRLTDRKVDLTTEGLDLAFFLGEPEDSTLRIRKIADVERVLCAAPDYVAARGMP
AGGAELTSGAHECLNLRFPGATEFQWHLKTPDGPKRFRIQGRYESDDGDVLNDWALAGQGIVMKPIFEVAEHLKAGRLVA
VAQETPPMPIQMACLFTHRRMQDPKTRLFMEFVINRIGDLVRQAEKLGQLPR

Sequences:

>Translated_292_residues
MSAAGRDLRISPAVTSSRISQLEEHLSVRLFQRTTRSLTPTEHGKAFYSGAKEILESVENAEAQVIDITENPKGSLYVAA
PLGVGRRLIAPEVPGFLDLYPEVRVRLRLTDRKVDLTTEGLDLAFFLGEPEDSTLRIRKIADVERVLCAAPDYVAARGMP
AGGAELTSGAHECLNLRFPGATEFQWHLKTPDGPKRFRIQGRYESDDGDVLNDWALAGQGIVMKPIFEVAEHLKAGRLVA
VAQETPPMPIQMACLFTHRRMQDPKTRLFMEFVINRIGDLVRQAEKLGQLPR
>Mature_291_residues
SAAGRDLRISPAVTSSRISQLEEHLSVRLFQRTTRSLTPTEHGKAFYSGAKEILESVENAEAQVIDITENPKGSLYVAAP
LGVGRRLIAPEVPGFLDLYPEVRVRLRLTDRKVDLTTEGLDLAFFLGEPEDSTLRIRKIADVERVLCAAPDYVAARGMPA
GGAELTSGAHECLNLRFPGATEFQWHLKTPDGPKRFRIQGRYESDDGDVLNDWALAGQGIVMKPIFEVAEHLKAGRLVAV
AQETPPMPIQMACLFTHRRMQDPKTRLFMEFVINRIGDLVRQAEKLGQLPR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=273, Percent_Identity=32.967032967033, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789440, Length=244, Percent_Identity=31.1475409836066, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=30.1038062283737, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=26.643598615917, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693193, Length=263, Percent_Identity=28.5171102661597, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=27.5735294117647, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787128, Length=272, Percent_Identity=26.8382352941176, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789173, Length=239, Percent_Identity=27.1966527196653, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=151, Percent_Identity=27.1523178807947, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32470; Mature: 32339

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAGRDLRISPAVTSSRISQLEEHLSVRLFQRTTRSLTPTEHGKAFYSGAKEILESVEN
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
AEAQVIDITENPKGSLYVAAPLGVGRRLIAPEVPGFLDLYPEVRVRLRLTDRKVDLTTEG
CCEEEEEECCCCCCCEEEEECCCCCCEECCCCCCCHHHCCCCEEEEEEEECCEEEEEECC
LDLAFFLGEPEDSTLRIRKIADVERVLCAAPDYVAARGMPAGGAELTSGAHECLNLRFPG
CEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHCCHHHHEEEECCC
ATEFQWHLKTPDGPKRFRIQGRYESDDGDVLNDWALAGQGIVMKPIFEVAEHLKAGRLVA
CCCEEEEEECCCCCCEEEEEECEECCCCCCHHHHHHCCCCEEHHHHHHHHHHHCCCCEEE
VAQETPPMPIQMACLFTHRRMQDPKTRLFMEFVINRIGDLVRQAEKLGQLPR
EECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAAGRDLRISPAVTSSRISQLEEHLSVRLFQRTTRSLTPTEHGKAFYSGAKEILESVEN
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
AEAQVIDITENPKGSLYVAAPLGVGRRLIAPEVPGFLDLYPEVRVRLRLTDRKVDLTTEG
CCEEEEEECCCCCCCEEEEECCCCCCEECCCCCCCHHHCCCCEEEEEEEECCEEEEEECC
LDLAFFLGEPEDSTLRIRKIADVERVLCAAPDYVAARGMPAGGAELTSGAHECLNLRFPG
CEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHCCHHHHEEEECCC
ATEFQWHLKTPDGPKRFRIQGRYESDDGDVLNDWALAGQGIVMKPIFEVAEHLKAGRLVA
CCCEEEEEECCCCCCEEEEEECEECCCCCCHHHHHHCCCCEEHHHHHHHHHHHCCCCEEE
VAQETPPMPIQMACLFTHRRMQDPKTRLFMEFVINRIGDLVRQAEKLGQLPR
EECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]