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The map label for this gene is eutB [H]

Identifier: 89055068

GI number: 89055068

Start: 2586875

End: 2588251

Strand: Reverse

Name: eutB [H]

Synonym: Jann_2577

Alternate gene names: 89055068

Gene position: 2588251-2586875 (Counterclockwise)

Preceding gene: 89055070

Following gene: 89055067

Centisome position: 59.94

GC content: 65.0

Gene sequence:

>1377_bases
ATGGGCTACCGGACAGACATAAGCGGCACGCGCTACACCTTCGCCTCGCTCAAGCGGCTGTTGGCGAAGGCCTCGCCCGA
GCGGAGCGGGGACCATCTGGCTGGGTTGGCCGCCGATAGCCCGTTGGAGCGGTTGGCGGCACAGATGTGTCTGGCCGATG
CGCCTCTGCGCGATTTTCTGGACGATGTGCTGGAGGTTGACGGGGATGCGGTCTCTCGCCTGATCGCTGACCAGCATGAC
GCGCAGGCGTTCGAGAGCATAAGCGCGCTGACCGTGGGCGGGTTCCGGGACTGGCTGCTGGACCCTGGCGTCACCGGCGT
GGAACTGGCCCAGATCGCATGGGGTCTGACGCCCGAGATGGTGGCGGCTGTCTCCAAGATCATGCGGGCGCAGGATCTGA
TCGCGGTTGCCGCCAAGATCGAGGTCGTGACGCGGTTCCGGTCGACCATTGGCCTGCGCGGACGGCTCTCCACCCGCAAC
CAACCCAATCACCCCACCGATGACAGCCGGGGGATCGCGTTGTCGGCCCTGGACGGGCTGCTGATGGGGGCAGGGGATGC
TGTGATCGGGGTGAACCCAGCCACCGATAGTGTGGAAGACTTCACCCGCGTATGCGAGGTGTTGGAGGCGATCAGAACCC
AGTTGGACATCCCCACGCAGCATTGCTGTCTGGGTCACGTCACCACCGCTTTGGCTGCGATGGAGCGCGGCGCGCCGGTG
GATCTTGTGTTCCAGTCTGTCGCGGGCAGCCAGGCGGCGAACGACGGCTTCGGCGTCACGCTGGAGATGCTGGACGAGGC
CCACGCCGCCGCCCGCGCGTTGGGCCGAGCGCCCGACGGCGCCAACCTGATGTATTTCGAGACGGGGCAAGGTGCAGCCC
TGTCAGCGAACGCCCATCACGGCATGGACCAGCAAACGATGGAGACCCGCGCCTATGCCGTCGCACGCCGCTACAAGCCG
CTGCTGGTCAACACGGTCGTGGGTTTCATCGGGCCGGAATACCTCGCGGACGGGAAACAGATCATCCGCGCGGGGTTGGA
GGATCACTTCTGCGGCAAGCTCCTGGGTCTGCCGATGGGGGTGGATGTGTGCTACACCAACCATTCCTACGCCGATCAGG
AAGACATGGATATCCTGCTGACGACCCTTGCCGCGGCGGGCGTCAGTTTCGTAATCACCGTGCCGGGGGCCGATGATATC
ATGCTGAATTACCAATCCCTGTCTTTCCACGATGCCCACTTCATCCGGGACACCCTGGGTCGCCCGCCGGCCCCAGAATT
TGCCGCCTGGATGGAGGCCAACGGCATCGGCTCTGCGGATGTGCGGTTGCCGCCGTTGGAGACGGCTCTGGCGCAATTGA
GGCTGCCGGGATCATGA

Upstream 100 bases:

>100_bases
TGCGGCACGGCGAGTAACATTTGCATGAGACACCCGTGAGCGCGCGTGCTTCCCCGGCCGTGATTTGCGTGTAAGCTGGT
GCCAAATCAAGGACATGGCC

Downstream 100 bases:

>100_bases
GTGACCATCTGCCCGGGCACCTATCCGCCATCACGCAAGCGCGCCTGACCCTCGGGCCGCGCCGCCGCACGCAGGACACG
CGCACGGCGCTAGCCTTTGC

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MGYRTDISGTRYTFASLKRLLAKASPERSGDHLAGLAADSPLERLAAQMCLADAPLRDFLDDVLEVDGDAVSRLIADQHD
AQAFESISALTVGGFRDWLLDPGVTGVELAQIAWGLTPEMVAAVSKIMRAQDLIAVAAKIEVVTRFRSTIGLRGRLSTRN
QPNHPTDDSRGIALSALDGLLMGAGDAVIGVNPATDSVEDFTRVCEVLEAIRTQLDIPTQHCCLGHVTTALAAMERGAPV
DLVFQSVAGSQAANDGFGVTLEMLDEAHAAARALGRAPDGANLMYFETGQGAALSANAHHGMDQQTMETRAYAVARRYKP
LLVNTVVGFIGPEYLADGKQIIRAGLEDHFCGKLLGLPMGVDVCYTNHSYADQEDMDILLTTLAAAGVSFVITVPGADDI
MLNYQSLSFHDAHFIRDTLGRPPAPEFAAWMEANGIGSADVRLPPLETALAQLRLPGS

Sequences:

>Translated_458_residues
MGYRTDISGTRYTFASLKRLLAKASPERSGDHLAGLAADSPLERLAAQMCLADAPLRDFLDDVLEVDGDAVSRLIADQHD
AQAFESISALTVGGFRDWLLDPGVTGVELAQIAWGLTPEMVAAVSKIMRAQDLIAVAAKIEVVTRFRSTIGLRGRLSTRN
QPNHPTDDSRGIALSALDGLLMGAGDAVIGVNPATDSVEDFTRVCEVLEAIRTQLDIPTQHCCLGHVTTALAAMERGAPV
DLVFQSVAGSQAANDGFGVTLEMLDEAHAAARALGRAPDGANLMYFETGQGAALSANAHHGMDQQTMETRAYAVARRYKP
LLVNTVVGFIGPEYLADGKQIIRAGLEDHFCGKLLGLPMGVDVCYTNHSYADQEDMDILLTTLAAAGVSFVITVPGADDI
MLNYQSLSFHDAHFIRDTLGRPPAPEFAAWMEANGIGSADVRLPPLETALAQLRLPGS
>Mature_457_residues
GYRTDISGTRYTFASLKRLLAKASPERSGDHLAGLAADSPLERLAAQMCLADAPLRDFLDDVLEVDGDAVSRLIADQHDA
QAFESISALTVGGFRDWLLDPGVTGVELAQIAWGLTPEMVAAVSKIMRAQDLIAVAAKIEVVTRFRSTIGLRGRLSTRNQ
PNHPTDDSRGIALSALDGLLMGAGDAVIGVNPATDSVEDFTRVCEVLEAIRTQLDIPTQHCCLGHVTTALAAMERGAPVD
LVFQSVAGSQAANDGFGVTLEMLDEAHAAARALGRAPDGANLMYFETGQGAALSANAHHGMDQQTMETRAYAVARRYKPL
LVNTVVGFIGPEYLADGKQIIRAGLEDHFCGKLLGLPMGVDVCYTNHSYADQEDMDILLTTLAAAGVSFVITVPGADDIM
LNYQSLSFHDAHFIRDTLGRPPAPEFAAWMEANGIGSADVRLPPLETALAQLRLPGS

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=437, Percent_Identity=47.8260869565217, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 48809; Mature: 48678

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYRTDISGTRYTFASLKRLLAKASPERSGDHLAGLAADSPLERLAAQMCLADAPLRDFL
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHH
DDVLEVDGDAVSRLIADQHDAQAFESISALTVGGFRDWLLDPGVTGVELAQIAWGLTPEM
HHHHHCCHHHHHHHHHCHHHHHHHHHHHHHEECCHHHHHCCCCCCHHHHHHHHHCCCHHH
VAAVSKIMRAQDLIAVAAKIEVVTRFRSTIGLRGRLSTRNQPNHPTDDSRGIALSALDGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH
LMGAGDAVIGVNPATDSVEDFTRVCEVLEAIRTQLDIPTQHCCLGHVTTALAAMERGAPV
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCH
DLVFQSVAGSQAANDGFGVTLEMLDEAHAAARALGRAPDGANLMYFETGQGAALSANAHH
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCC
GMDQQTMETRAYAVARRYKPLLVNTVVGFIGPEYLADGKQIIRAGLEDHFCGKLLGLPMG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCHHHHHHHCCCHHHHHHHHCCCCC
VDVCYTNHSYADQEDMDILLTTLAAAGVSFVITVPGADDIMLNYQSLSFHDAHFIRDTLG
CEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCC
RPPAPEFAAWMEANGIGSADVRLPPLETALAQLRLPGS
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
GYRTDISGTRYTFASLKRLLAKASPERSGDHLAGLAADSPLERLAAQMCLADAPLRDFL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHH
DDVLEVDGDAVSRLIADQHDAQAFESISALTVGGFRDWLLDPGVTGVELAQIAWGLTPEM
HHHHHCCHHHHHHHHHCHHHHHHHHHHHHHEECCHHHHHCCCCCCHHHHHHHHHCCCHHH
VAAVSKIMRAQDLIAVAAKIEVVTRFRSTIGLRGRLSTRNQPNHPTDDSRGIALSALDGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH
LMGAGDAVIGVNPATDSVEDFTRVCEVLEAIRTQLDIPTQHCCLGHVTTALAAMERGAPV
HHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCH
DLVFQSVAGSQAANDGFGVTLEMLDEAHAAARALGRAPDGANLMYFETGQGAALSANAHH
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCC
GMDQQTMETRAYAVARRYKPLLVNTVVGFIGPEYLADGKQIIRAGLEDHFCGKLLGLPMG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCHHHHHHHCCCHHHHHHHHCCCCC
VDVCYTNHSYADQEDMDILLTTLAAAGVSFVITVPGADDIMLNYQSLSFHDAHFIRDTLG
CEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCC
RPPAPEFAAWMEANGIGSADVRLPPLETALAQLRLPGS
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]