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The map label for this gene is metH [H]

Identifier: 89055038

GI number: 89055038

Start: 2550489

End: 2551511

Strand: Direct

Name: metH [H]

Synonym: Jann_2547

Alternate gene names: 89055038

Gene position: 2550489-2551511 (Clockwise)

Preceding gene: 89055037

Following gene: 89055039

Centisome position: 59.07

GC content: 65.3

Gene sequence:

>1023_bases
ATGACCAATGCCCTCTCCCGTATGCTGTCCACCCGAGATTGGCTTCTGGCGGATGGCGCGACGGGCACAACGTTGTTCAA
CATGGGGCTGCAATCGGGCGACGCGCCGGAATTGTGGAACGTCGACCATCCCGACCGGATCACCCAACTTTACAAGGGCG
CGGTCGATGCGGGCTCGGACCTGTTTCTGACCAATTCCTTCGGCGGCACGGCGGCCCGGTTGAAGCTGCACGACGCGCAG
ACCCGCGTGTTCGAGCTGAACAAAGCCGCCGCCGAGATTGGCCGCGAGGTGGCCGACAGCGCCGGTCACGATGTGATCGT
AGCGGGCTCCGTCGGTCCGACCGGAGAGATCATGGTGCCCATGGGCGCGCTGACCCACGCGCTGGCGGTTGAGATGTTTC
ACGAACAGGCGGAGGGTCTGAAGGCCGGCGGCGCGGATGTGCTGTGGGCCGAGACGATCTCCGCCCCCGACGAATACAAA
GCCGCATCTGAGGCCGCGAAACTGGCCGAGATGCCATGGTGCGGCACGATGAGTTTCGACACCGCAGGCCGCACGATGAT
GGGCCTGACATCGGCTGCGATGTCGTCAATGGTTGAGAAACTTGACCATGCACCGCTGGCATATGGCGCAAACTGCGGCG
TCGGCGCATCGGATCTGTTGCGGACAATCTTGGGGTTCCGCGCCTCGGGGTCAGAGCGGCCCGTGATCGCCAAGGGCAAC
GCTGGCATCCCGAAATACGTGGACGGCCATATCCATTACGACGGCACGCCCGAATTGATGGCCCGCTACGCCGTCCTGGC
CCGCGATTGCGGCGCGACGATCATCGGCGGGTGTTGTGGCACGACGCCAGAGCATCTGGAGAAGATGCGCGCGGCGCTGG
AGACGGTGCCCCGCGGCAATGCCCCAAGCCTGGAACAGATCGCCAAGGAACTGGGCGGCTTCTCATCGGGCGTCGACGGC
ACGGAGGAAGGCGGCGGCGGCCCGACCCGCGAACGTCGCGGCCGCAGACGCGTCCGCAATTGA

Upstream 100 bases:

>100_bases
AGCACGGCTCATGAAAATGATGCATACTTTCTATCTTGCTCATAACGCGCGTCCGGGACATGCCTCGGGCGCGCGTCTCT
CATAGCAAGGCCTTAACCCC

Downstream 100 bases:

>100_bases
TCCGTTTGTTTACGTGCCTTTAACCAATCTCTGGTGCTCTGCATGGTATGGATGATCTGTTTTCCCTCGCCGCACCCGTG
CCCGCTACTATCGAGGCCGA

Product: methionine synthase I

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTNALSRMLSTRDWLLADGATGTTLFNMGLQSGDAPELWNVDHPDRITQLYKGAVDAGSDLFLTNSFGGTAARLKLHDAQ
TRVFELNKAAAEIGREVADSAGHDVIVAGSVGPTGEIMVPMGALTHALAVEMFHEQAEGLKAGGADVLWAETISAPDEYK
AASEAAKLAEMPWCGTMSFDTAGRTMMGLTSAAMSSMVEKLDHAPLAYGANCGVGASDLLRTILGFRASGSERPVIAKGN
AGIPKYVDGHIHYDGTPELMARYAVLARDCGATIIGGCCGTTPEHLEKMRAALETVPRGNAPSLEQIAKELGGFSSGVDG
TEEGGGGPTRERRGRRRVRN

Sequences:

>Translated_340_residues
MTNALSRMLSTRDWLLADGATGTTLFNMGLQSGDAPELWNVDHPDRITQLYKGAVDAGSDLFLTNSFGGTAARLKLHDAQ
TRVFELNKAAAEIGREVADSAGHDVIVAGSVGPTGEIMVPMGALTHALAVEMFHEQAEGLKAGGADVLWAETISAPDEYK
AASEAAKLAEMPWCGTMSFDTAGRTMMGLTSAAMSSMVEKLDHAPLAYGANCGVGASDLLRTILGFRASGSERPVIAKGN
AGIPKYVDGHIHYDGTPELMARYAVLARDCGATIIGGCCGTTPEHLEKMRAALETVPRGNAPSLEQIAKELGGFSSGVDG
TEEGGGGPTRERRGRRRVRN
>Mature_339_residues
TNALSRMLSTRDWLLADGATGTTLFNMGLQSGDAPELWNVDHPDRITQLYKGAVDAGSDLFLTNSFGGTAARLKLHDAQT
RVFELNKAAAEIGREVADSAGHDVIVAGSVGPTGEIMVPMGALTHALAVEMFHEQAEGLKAGGADVLWAETISAPDEYKA
ASEAAKLAEMPWCGTMSFDTAGRTMMGLTSAAMSSMVEKLDHAPLAYGANCGVGASDLLRTILGFRASGSERPVIAKGNA
GIPKYVDGHIHYDGTPELMARYAVLARDCGATIIGGCCGTTPEHLEKMRAALETVPRGNAPSLEQIAKELGGFSSGVDGT
EEGGGGPTRERRGRRRVRN

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=284, Percent_Identity=30.6338028169014, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI13162290, Length=331, Percent_Identity=25.9818731117825, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790450, Length=328, Percent_Identity=28.9634146341463, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=322, Percent_Identity=31.9875776397516, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 35721; Mature: 35590

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNALSRMLSTRDWLLADGATGTTLFNMGLQSGDAPELWNVDHPDRITQLYKGAVDAGSD
CCHHHHHHHHCCCEEEECCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
LFLTNSFGGTAARLKLHDAQTRVFELNKAAAEIGREVADSAGHDVIVAGSVGPTGEIMVP
EEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEC
MGALTHALAVEMFHEQAEGLKAGGADVLWAETISAPDEYKAASEAAKLAEMPWCGTMSFD
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TAGRTMMGLTSAAMSSMVEKLDHAPLAYGANCGVGASDLLRTILGFRASGSERPVIAKGN
CCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCC
AGIPKYVDGHIHYDGTPELMARYAVLARDCGATIIGGCCGTTPEHLEKMRAALETVPRGN
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCC
APSLEQIAKELGGFSSGVDGTEEGGGGPTRERRGRRRVRN
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TNALSRMLSTRDWLLADGATGTTLFNMGLQSGDAPELWNVDHPDRITQLYKGAVDAGSD
CHHHHHHHHCCCEEEECCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
LFLTNSFGGTAARLKLHDAQTRVFELNKAAAEIGREVADSAGHDVIVAGSVGPTGEIMVP
EEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEC
MGALTHALAVEMFHEQAEGLKAGGADVLWAETISAPDEYKAASEAAKLAEMPWCGTMSFD
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
TAGRTMMGLTSAAMSSMVEKLDHAPLAYGANCGVGASDLLRTILGFRASGSERPVIAKGN
CCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCC
AGIPKYVDGHIHYDGTPELMARYAVLARDCGATIIGGCCGTTPEHLEKMRAALETVPRGN
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCC
APSLEQIAKELGGFSSGVDGTEEGGGGPTRERRGRRRVRN
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA