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The map label for this gene is dctB [H]

Identifier: 89055033

GI number: 89055033

Start: 2546197

End: 2547963

Strand: Reverse

Name: dctB [H]

Synonym: Jann_2542

Alternate gene names: 89055033

Gene position: 2547963-2546197 (Counterclockwise)

Preceding gene: 89055034

Following gene: 89055032

Centisome position: 59.01

GC content: 64.52

Gene sequence:

>1767_bases
ATGAGCTTTGCTGATCGTCCCGCATCCCCGCCCCGGCGGGGCATTCGCCGCCCCTGGGTGCTGCGCGCGCTGATCGTGCT
GTTTCTGGGCGGTGCGATCGCGATCATCTGGATCTCCAACATCTGGCTGACAGAGCGGTTCACCGAGACGACGCGCAACC
GTGCGGAGGTGCGGCTGGCGCTCTATTCCGGCTCGGTCATGTCCGAGATCCGGCGCCATCAGGTTGTGCCCCTGCTCCTG
AGCCGCGACCCCTCCCTGATCCGGTCGCTGGAGGCCAATGACTACACACAGACCACGCAATATCTGATCTCCTACGTGGA
GGATATCGGCGCGGCGTCGATCCTGCTTCTGGACCGTGAGGGGCGGGTTGTGGCGGCGACAGACCGTGCGCGCATTTCCG
AGCGGCGCGACACGGACCCTTTCTTTGTTGAGGCCACACGGTCGCCGAACACCGTGTTCACCTCCAACGAGCCCGAAACG
GGGCGCTTTGATTTCACCTTCTCGCGCCGGATCGAGAACGGTGATGGCCTGCTGGGTGTCATCCTGGTGGAAATTGACCT
GGCCCGCCTGGTCGACCGCTGGCGCGGCGCGTCGGAGGCGGTGTTTCTGGCCAATTCCCAGGGCGATATCATCCTGTCGA
CAGAGGCGCGCTGGCGGGGCCGCACGGAGATTGAGGCGATGGCCCTGCGCGACCCGCCCTCGGCCATTCAGCGCGCGTTG
GAAGGGGCGGGGAACTGGGCGTTCGGCGACCCTGACCCCAACTTCTTCGGCGCGGCCACGATCCGGCTGGAGGCGCGTAT
CCCGTTTCGCGGGTGGCGCATCGTCAGCTACACGGCCTACACGTCGGTGCGGGAGCGGGTGAACGGGGTCCTGGCGCTGG
AGATTATGGGATTCGCCCTGGCGCTGGCCGCCGCATTTTACCTGCTGAGCCGTCAGGCCCGGTCACAGACGGCGGTGCTG
GCGCGCGAGTCGGCGGAGTTGCGCCGCCTCAACGGGCGCCTACAGCGAGAAATTGCCGAACGTCAAAGGGTTGAACGGAC
CCTGGAGGAGGCGGAACTGAGCCTTCAGCAGAGCCAAAAACTGGCCGCATTGGGGGAGATGTCGGCCGCCGTCTCGCACG
AGTTGAACCAGCCGCTGGCGGCGATGAAAACCTACCTCGCCGGGGCGCGGCTTTTGTTGCAGCGAAAACGCACCGAGGAG
GCCGCCTCCAGCTTCCAGCGGATCGATGACCTGATCGAGCGGATGGGCGCCATCACGCGGCAGCTGAAATCCTATGCCCG
CAAGGGCGGAGATGCCCTTGAGCCGGTCGATATGCGGGATGCTCTGAAGGGCGCTTTGACGATGATGGAGCCGCAATTGC
GCAGCGACACGGTCCACATCACGCAAAGCCTGCCGCGCAAGCCGGCCCTGGTCATGGCCGACCGTCTGCGGCTGGAGCAG
GTGATCATCAATTTGCTGCGCAATGCGCTGGACGCGATGGGCGGGGCCGATGAGAAGGAATTGGACCTGATTATCGTGGA
AGGTGACGAAGTTGTGTTGACCGTGCGCGACAGCGGACCGGGGATTGACGATCTGGATGCTTTGTTCGAGCCATTCTACA
CGACAAAGAAACCCGGAGAGGGGGTTGGTCTTGGGCTGGCCATCTCCTCGGGGATCGTGAAAGACCTTGGCGGGCGTCTG
TCGGCGCGCAACGGGGAACAGGAAGGCGCGGTGTTTGAGATCCGTCTGCCTGCGCTGGATGAGGCGCAGAGTGAAGCGGC
GGAATAA

Upstream 100 bases:

>100_bases
CGGCGCTGGTGGCGGCCTGACCCGGTGATCCGACGCCGCCGCGCCCGCATCCTGCGGGCCGCCCCTTGAGGCGCGTCGTG
GCGCGGCATAGTCTTCTTCC

Downstream 100 bases:

>100_bases
GGGATCTGCTGCGTGTAAAGGCTGGACACCGCGCGATGCGCTGCCCAACGGGCGGTAGCGCGTTAATACCCGCGCTGCGC
TGCCCGAAGGGTGGTCGCGC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSFADRPASPPRRGIRRPWVLRALIVLFLGGAIAIIWISNIWLTERFTETTRNRAEVRLALYSGSVMSEIRRHQVVPLLL
SRDPSLIRSLEANDYTQTTQYLISYVEDIGAASILLLDREGRVVAATDRARISERRDTDPFFVEATRSPNTVFTSNEPET
GRFDFTFSRRIENGDGLLGVILVEIDLARLVDRWRGASEAVFLANSQGDIILSTEARWRGRTEIEAMALRDPPSAIQRAL
EGAGNWAFGDPDPNFFGAATIRLEARIPFRGWRIVSYTAYTSVRERVNGVLALEIMGFALALAAAFYLLSRQARSQTAVL
ARESAELRRLNGRLQREIAERQRVERTLEEAELSLQQSQKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRKRTEE
AASSFQRIDDLIERMGAITRQLKSYARKGGDALEPVDMRDALKGALTMMEPQLRSDTVHITQSLPRKPALVMADRLRLEQ
VIINLLRNALDAMGGADEKELDLIIVEGDEVVLTVRDSGPGIDDLDALFEPFYTTKKPGEGVGLGLAISSGIVKDLGGRL
SARNGEQEGAVFEIRLPALDEAQSEAAE

Sequences:

>Translated_588_residues
MSFADRPASPPRRGIRRPWVLRALIVLFLGGAIAIIWISNIWLTERFTETTRNRAEVRLALYSGSVMSEIRRHQVVPLLL
SRDPSLIRSLEANDYTQTTQYLISYVEDIGAASILLLDREGRVVAATDRARISERRDTDPFFVEATRSPNTVFTSNEPET
GRFDFTFSRRIENGDGLLGVILVEIDLARLVDRWRGASEAVFLANSQGDIILSTEARWRGRTEIEAMALRDPPSAIQRAL
EGAGNWAFGDPDPNFFGAATIRLEARIPFRGWRIVSYTAYTSVRERVNGVLALEIMGFALALAAAFYLLSRQARSQTAVL
ARESAELRRLNGRLQREIAERQRVERTLEEAELSLQQSQKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRKRTEE
AASSFQRIDDLIERMGAITRQLKSYARKGGDALEPVDMRDALKGALTMMEPQLRSDTVHITQSLPRKPALVMADRLRLEQ
VIINLLRNALDAMGGADEKELDLIIVEGDEVVLTVRDSGPGIDDLDALFEPFYTTKKPGEGVGLGLAISSGIVKDLGGRL
SARNGEQEGAVFEIRLPALDEAQSEAAE
>Mature_587_residues
SFADRPASPPRRGIRRPWVLRALIVLFLGGAIAIIWISNIWLTERFTETTRNRAEVRLALYSGSVMSEIRRHQVVPLLLS
RDPSLIRSLEANDYTQTTQYLISYVEDIGAASILLLDREGRVVAATDRARISERRDTDPFFVEATRSPNTVFTSNEPETG
RFDFTFSRRIENGDGLLGVILVEIDLARLVDRWRGASEAVFLANSQGDIILSTEARWRGRTEIEAMALRDPPSAIQRALE
GAGNWAFGDPDPNFFGAATIRLEARIPFRGWRIVSYTAYTSVRERVNGVLALEIMGFALALAAAFYLLSRQARSQTAVLA
RESAELRRLNGRLQREIAERQRVERTLEEAELSLQQSQKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRKRTEEA
ASSFQRIDDLIERMGAITRQLKSYARKGGDALEPVDMRDALKGALTMMEPQLRSDTVHITQSLPRKPALVMADRLRLEQV
IINLLRNALDAMGGADEKELDLIIVEGDEVVLTVRDSGPGIDDLDALFEPFYTTKKPGEGVGLGLAISSGIVKDLGGRLS
ARNGEQEGAVFEIRLPALDEAQSEAAE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=31.6239316239316, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788549, Length=231, Percent_Identity=26.4069264069264, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081816, Length=260, Percent_Identity=31.1538461538462, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI48994928, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788393, Length=243, Percent_Identity=32.0987654320988, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 65284; Mature: 65153

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFADRPASPPRRGIRRPWVLRALIVLFLGGAIAIIWISNIWLTERFTETTRNRAEVRLA
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHCCHHEEEEE
LYSGSVMSEIRRHQVVPLLLSRDPSLIRSLEANDYTQTTQYLISYVEDIGAASILLLDRE
EECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
GRVVAATDRARISERRDTDPFFVEATRSPNTVFTSNEPETGRFDFTFSRRIENGDGLLGV
CCEEEECCHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCEEEE
ILVEIDLARLVDRWRGASEAVFLANSQGDIILSTEARWRGRTEIEAMALRDPPSAIQRAL
HHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHH
EGAGNWAFGDPDPNFFGAATIRLEARIPFRGWRIVSYTAYTSVRERVNGVLALEIMGFAL
HCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCEEHHHHHHHHH
ALAAAFYLLSRQARSQTAVLARESAELRRLNGRLQREIAERQRVERTLEEAELSLQQSQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRKRTEEAASSFQRIDDLIERMGAITR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLKSYARKGGDALEPVDMRDALKGALTMMEPQLRSDTVHITQSLPRKPALVMADRLRLEQ
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHH
VIINLLRNALDAMGGADEKELDLIIVEGDEVVLTVRDSGPGIDDLDALFEPFYTTKKPGE
HHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCC
GVGLGLAISSGIVKDLGGRLSARNGEQEGAVFEIRLPALDEAQSEAAE
CCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCC
>Mature Secondary Structure 
SFADRPASPPRRGIRRPWVLRALIVLFLGGAIAIIWISNIWLTERFTETTRNRAEVRLA
CCCCCCCCCHHHHCCCHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHCCHHEEEEE
LYSGSVMSEIRRHQVVPLLLSRDPSLIRSLEANDYTQTTQYLISYVEDIGAASILLLDRE
EECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
GRVVAATDRARISERRDTDPFFVEATRSPNTVFTSNEPETGRFDFTFSRRIENGDGLLGV
CCEEEECCHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCEEEE
ILVEIDLARLVDRWRGASEAVFLANSQGDIILSTEARWRGRTEIEAMALRDPPSAIQRAL
HHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHH
EGAGNWAFGDPDPNFFGAATIRLEARIPFRGWRIVSYTAYTSVRERVNGVLALEIMGFAL
HCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHCCEEHHHHHHHHH
ALAAAFYLLSRQARSQTAVLARESAELRRLNGRLQREIAERQRVERTLEEAELSLQQSQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRKRTEEAASSFQRIDDLIERMGAITR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLKSYARKGGDALEPVDMRDALKGALTMMEPQLRSDTVHITQSLPRKPALVMADRLRLEQ
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHH
VIINLLRNALDAMGGADEKELDLIIVEGDEVVLTVRDSGPGIDDLDALFEPFYTTKKPGE
HHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCC
GVGLGLAISSGIVKDLGGRLSARNGEQEGAVFEIRLPALDEAQSEAAE
CCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]