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The map label for this gene is dctD [H]

Identifier: 89055032

GI number: 89055032

Start: 2544718

End: 2546034

Strand: Reverse

Name: dctD [H]

Synonym: Jann_2541

Alternate gene names: 89055032

Gene position: 2546034-2544718 (Counterclockwise)

Preceding gene: 89055033

Following gene: 89055031

Centisome position: 58.96

GC content: 63.1

Gene sequence:

>1317_bases
ATGAAAATCGCGATTGTAGATGATGAGCAGGACATGCGGCAGTCGATCAGCCAGTGGCTGGCGCTGTCGGGCTTTGACAC
CGAAACCTATTCCAGCGCGGAAGAGGCATTGAAGGCCATCGGCTCGGATTTTCCGGGCGTTGTGGTCACGGACGTGAAAA
TGCCCGGCATGGACGGCATGGCCTTCCTGAAGCGGCTGATGGGTGTGGATAGCGGCATCCCGGTCATCCTGATCACGGGC
CACGGCGATGTGCCGATGGCGGTGGAGGCGATGCGGATCGGGGCCTATGATTTCTTGGAAAAGCCGTTCAACCCGGATCG
GATGACGGATCTGGCCAAGCGGGCGGGGCAGCAGCGCCGCTTGACGCTGGACAACCGTGCCCTGCGGCGGGAACTCAGCG
ACGGCACCGTTTTGATGCGCAAGCTGATCGGGTCGAGCCCGGTGATGGAACGGTTGCGCGAAGATATTCTGGATCTTGGC
CAGGCCGACGGTCATGTGCTGATCACCGGCGAGACGGGCACCGGCAAAACGCTTGTCGCCCATGCGCTGCATGCCGTGGG
TCCGCGCGCGGGTAAGCATTTCGTCGTGCTGTCCTGTGCGGCCCATGAGGAGGAGGAGCTGGCCTCACAACTCTTCGGTC
CCGTCGGCGAAGATCAGGACAAGCCGCTGGTGGAGCAGGCGCGCGGCGGCACGCTGGTGCTGGAGGATGTGGAGGCGCTG
TCGCAAGCCCTGCAAGCGCGCCTTCTGACGTTCATCAACGATCAGGGCACGCCGGCCGAGACGCGCATCGTGGCCATTTG
CAACGCGCCCGAGCCCGATCAATGCGAAGGCGCGCTCCGGCCCGATCTGTTCTACCGCCTTGCCGCCATGCGGATCGATC
TGCCGCCCCTGCGCCAGCGGGGCGAGGATATCCTGACGCTGTTCACCCGCTTCGGGGAGCAATTCAGCGAGGAATACGGG
ACGGAAGCCCCCCAAGTCACCGCGCAGGAAGCGGCACAGCTTCTGCAAGCGCCCTGGCCCGGCAATGTGCGCCAACTGAT
CAACATCTCCGAGCGCGCGATCCTGCAACAAAGGCGTGGGTCGGGGTCCATCGCGTCGCTGCTGATGAGCGACAACACCC
AGATCGCACCGCAGGCGATGGGGGAGGGCAAGCCCCTGAAGGAATATGTGGAGGCGTTCGAGAGGATGCTGATCGATAAC
ACCATGCGGCGTCATCGCGGCTCCATCGCGAGTGTCATGGATGAGTTGTGCCTGCCGCGCCGGACGTTGAATGAGAAGAT
GGCAAAGTATGGGTTGAGCCGGTCCGATTATCTGTGA

Upstream 100 bases:

>100_bases
TTAATACCCGCGCTGCGCTGCCCGAAGGGTGGTCGCGCATGAATACCCGCGCTCAAGTAGCCCGAAGGGCGGTAGCGCAT
AAAAAAAGGACACCTTCGCC

Downstream 100 bases:

>100_bases
AGGGCCGTGGTTGCAAGGTTGCAACCGGGGGTTAAGCCGCTGAAATACAATCCATATCCTGATCTCTTTAACCTTTTGCG
GGGCGTTTCGTGATGGGGGA

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKIAIVDDEQDMRQSISQWLALSGFDTETYSSAEEALKAIGSDFPGVVVTDVKMPGMDGMAFLKRLMGVDSGIPVILITG
HGDVPMAVEAMRIGAYDFLEKPFNPDRMTDLAKRAGQQRRLTLDNRALRRELSDGTVLMRKLIGSSPVMERLREDILDLG
QADGHVLITGETGTGKTLVAHALHAVGPRAGKHFVVLSCAAHEEEELASQLFGPVGEDQDKPLVEQARGGTLVLEDVEAL
SQALQARLLTFINDQGTPAETRIVAICNAPEPDQCEGALRPDLFYRLAAMRIDLPPLRQRGEDILTLFTRFGEQFSEEYG
TEAPQVTAQEAAQLLQAPWPGNVRQLINISERAILQQRRGSGSIASLLMSDNTQIAPQAMGEGKPLKEYVEAFERMLIDN
TMRRHRGSIASVMDELCLPRRTLNEKMAKYGLSRSDYL

Sequences:

>Translated_438_residues
MKIAIVDDEQDMRQSISQWLALSGFDTETYSSAEEALKAIGSDFPGVVVTDVKMPGMDGMAFLKRLMGVDSGIPVILITG
HGDVPMAVEAMRIGAYDFLEKPFNPDRMTDLAKRAGQQRRLTLDNRALRRELSDGTVLMRKLIGSSPVMERLREDILDLG
QADGHVLITGETGTGKTLVAHALHAVGPRAGKHFVVLSCAAHEEEELASQLFGPVGEDQDKPLVEQARGGTLVLEDVEAL
SQALQARLLTFINDQGTPAETRIVAICNAPEPDQCEGALRPDLFYRLAAMRIDLPPLRQRGEDILTLFTRFGEQFSEEYG
TEAPQVTAQEAAQLLQAPWPGNVRQLINISERAILQQRRGSGSIASLLMSDNTQIAPQAMGEGKPLKEYVEAFERMLIDN
TMRRHRGSIASVMDELCLPRRTLNEKMAKYGLSRSDYL
>Mature_438_residues
MKIAIVDDEQDMRQSISQWLALSGFDTETYSSAEEALKAIGSDFPGVVVTDVKMPGMDGMAFLKRLMGVDSGIPVILITG
HGDVPMAVEAMRIGAYDFLEKPFNPDRMTDLAKRAGQQRRLTLDNRALRRELSDGTVLMRKLIGSSPVMERLREDILDLG
QADGHVLITGETGTGKTLVAHALHAVGPRAGKHFVVLSCAAHEEEELASQLFGPVGEDQDKPLVEQARGGTLVLEDVEAL
SQALQARLLTFINDQGTPAETRIVAICNAPEPDQCEGALRPDLFYRLAAMRIDLPPLRQRGEDILTLFTRFGEQFSEEYG
TEAPQVTAQEAAQLLQAPWPGNVRQLINISERAILQQRRGSGSIASLLMSDNTQIAPQAMGEGKPLKEYVEAFERMLIDN
TMRRHRGSIASVMDELCLPRRTLNEKMAKYGLSRSDYL

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=32.9004329004329, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=32.4786324786325, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=30.6843267108168, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=28.6975717439294, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787583, Length=307, Percent_Identity=34.2019543973941, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=31.055900621118, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87082152, Length=297, Percent_Identity=32.6599326599327, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=30.5810397553517, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1786524, Length=300, Percent_Identity=33, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=30.4985337243402, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081872, Length=234, Percent_Identity=34.6153846153846, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081858, Length=287, Percent_Identity=26.8292682926829, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=104, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48257; Mature: 48257

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIVDDEQDMRQSISQWLALSGFDTETYSSAEEALKAIGSDFPGVVVTDVKMPGMDGM
CEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHH
AFLKRLMGVDSGIPVILITGHGDVPMAVEAMRIGAYDFLEKPFNPDRMTDLAKRAGQQRR
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCHHHHHHHHHCCCCCE
LTLDNRALRRELSDGTVLMRKLIGSSPVMERLREDILDLGQADGHVLITGETGTGKTLVA
ECHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
HALHAVGPRAGKHFVVLSCAAHEEEELASQLFGPVGEDQDKPLVEQARGGTLVLEDVEAL
HHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEHHHHHHH
SQALQARLLTFINDQGTPAETRIVAICNAPEPDQCEGALRPDLFYRLAAMRIDLPPLRQR
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHC
GEDILTLFTRFGEQFSEEYGTEAPQVTAQEAAQLLQAPWPGNVRQLINISERAILQQRRG
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
SGSIASLLMSDNTQIAPQAMGEGKPLKEYVEAFERMLIDNTMRRHRGSIASVMDELCLPR
CCHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
RTLNEKMAKYGLSRSDYL
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIAIVDDEQDMRQSISQWLALSGFDTETYSSAEEALKAIGSDFPGVVVTDVKMPGMDGM
CEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHH
AFLKRLMGVDSGIPVILITGHGDVPMAVEAMRIGAYDFLEKPFNPDRMTDLAKRAGQQRR
HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCHHHHHHCCCCCHHHHHHHHHCCCCCE
LTLDNRALRRELSDGTVLMRKLIGSSPVMERLREDILDLGQADGHVLITGETGTGKTLVA
ECHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
HALHAVGPRAGKHFVVLSCAAHEEEELASQLFGPVGEDQDKPLVEQARGGTLVLEDVEAL
HHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEHHHHHHH
SQALQARLLTFINDQGTPAETRIVAICNAPEPDQCEGALRPDLFYRLAAMRIDLPPLRQR
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHC
GEDILTLFTRFGEQFSEEYGTEAPQVTAQEAAQLLQAPWPGNVRQLINISERAILQQRRG
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
SGSIASLLMSDNTQIAPQAMGEGKPLKEYVEAFERMLIDNTMRRHRGSIASVMDELCLPR
CCHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
RTLNEKMAKYGLSRSDYL
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]