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The map label for this gene is prfB

Identifier: 89055024

GI number: 89055024

Start: 2532747

End: 2533874

Strand: Reverse

Name: prfB

Synonym: Jann_2533

Alternate gene names: 89055024

Gene position: 2533874-2532747 (Counterclockwise)

Preceding gene: 89055025

Following gene: 89055023

Centisome position: 58.68

GC content: 60.28

Gene sequence:

>1128_bases
ATGCGGGCCGAGACACAAGCCAATATCGAGAAGATCCGCAATTCCCTGACCCTTCTGGGGCAGCGGATGGACATAGAGAC
CGCGCCCCACCGGTTGGAGGAATTCAACGCCCGCGTCGAAGATCCGAACCTTTGGAATGATCCTGAGAAGGCGCAGAAAT
TGATGCGCGACCGGCAGGTGCTCGTCGACAAGATGAAGCAGTACGAGACGATCAGGCAGGGTGTGGACGACAACGCCGAG
CTGATCGAGCTGGGAGAGATGGAGGACGATGCCGAGGTCGTGGCCGAGGCCGAAGCGGCTTTGACGGCGCTGGTTGACGA
GGCCGCCGCCAAGGAGCTTGAGGCGCTGCTGGATGGGGAGGCCGACAGCAACGACACGTTTCTTGAGATCAATTCGGGCG
CGGGGGGCACGGAAAGCTGTGACTGGGCGTCGATGCTGGCGCGGATGTACACGCGGTGGGCCGAGAAGCAGGGCTATGAC
GTGGATCTGCAAAGCTTCAGCGCGGGCGAAGAGGCGGGGATCAAATCCGCCGTCTACAAGATCAGCGGGCACAACGCCTA
TGGTTGGTTGAAGTCCGAGAGCGGCGTGCACCGTCTGGTACGGATTTCACCTTTCGACAGCGCCGCCAAGCGGCACACGT
CTTTTACGTCGGTGAAGGTTTATCCGGTCGTGGACGACAACATCGAGATTGAGGTAAACCCCTCTGACATCCGCATCGAC
ACCTATCGCTCGTCCGGGGCCGGTGGCCAGCACGTGAACACCACGGATTCCGCGGTTCGTATCACCCACCATCCGACCGG
TATCGTGGTCACAAGTTCGGAGAAATCCCAACACCAGAACCGCGATATCGCCATGAAGGCGTTGAAATCGCGGCTCTATC
AGATGGAACTGGATAAGCGCTCGGCCAAGGTGAACGAGGTCCATGAGAATGCGGGCGATGCGGGCTGGGGCAACCAGATT
CGATCCTATGTGTTGCAGCCCTACCAGATGGTGAAGGACCTGCGGACGGCTTTTGAAACCTCCGACACGCAAGGGGTGCT
GGACGGCAATATCGATGGGCTGATGGCGAGCGTCTTGGCGATGGACGTGTCCGGCAAGAGCCGGGCCGAGGCCCGGGGTG
ATGACTGA

Upstream 100 bases:

>100_bases
GCCTTGCAAGGCTTTGGGACGCCCGGAGGGCGCGGTCGTGCATTGAAGGTCGGGGGGCGCTGGTCTATCCGTTGGGAAAC
GGAACACCAGGGGGGCGGAC

Downstream 100 bases:

>100_bases
GGCGGGGCCGTGGGGCCGCTTCATCGTCCATTGCGCACGGGGCGGGGACCGGAATTTGAGTTGTATTTGCCCAGATGAAG
GAGGGAGCAGCCGTGGCGCT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRAETQANIEKIRNSLTLLGQRMDIETAPHRLEEFNARVEDPNLWNDPEKAQKLMRDRQVLVDKMKQYETIRQGVDDNAE
LIELGEMEDDAEVVAEAEAALTALVDEAAAKELEALLDGEADSNDTFLEINSGAGGTESCDWASMLARMYTRWAEKQGYD
VDLQSFSAGEEAGIKSAVYKISGHNAYGWLKSESGVHRLVRISPFDSAAKRHTSFTSVKVYPVVDDNIEIEVNPSDIRID
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQMELDKRSAKVNEVHENAGDAGWGNQI
RSYVLQPYQMVKDLRTAFETSDTQGVLDGNIDGLMASVLAMDVSGKSRAEARGDD

Sequences:

>Translated_375_residues
MRAETQANIEKIRNSLTLLGQRMDIETAPHRLEEFNARVEDPNLWNDPEKAQKLMRDRQVLVDKMKQYETIRQGVDDNAE
LIELGEMEDDAEVVAEAEAALTALVDEAAAKELEALLDGEADSNDTFLEINSGAGGTESCDWASMLARMYTRWAEKQGYD
VDLQSFSAGEEAGIKSAVYKISGHNAYGWLKSESGVHRLVRISPFDSAAKRHTSFTSVKVYPVVDDNIEIEVNPSDIRID
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQMELDKRSAKVNEVHENAGDAGWGNQI
RSYVLQPYQMVKDLRTAFETSDTQGVLDGNIDGLMASVLAMDVSGKSRAEARGDD
>Mature_375_residues
MRAETQANIEKIRNSLTLLGQRMDIETAPHRLEEFNARVEDPNLWNDPEKAQKLMRDRQVLVDKMKQYETIRQGVDDNAE
LIELGEMEDDAEVVAEAEAALTALVDEAAAKELEALLDGEADSNDTFLEINSGAGGTESCDWASMLARMYTRWAEKQGYD
VDLQSFSAGEEAGIKSAVYKISGHNAYGWLKSESGVHRLVRISPFDSAAKRHTSFTSVKVYPVVDDNIEIEVNPSDIRID
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQMELDKRSAKVNEVHENAGDAGWGNQI
RSYVLQPYQMVKDLRTAFETSDTQGVLDGNIDGLMASVLAMDVSGKSRAEARGDD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=243, Percent_Identity=37.037037037037, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI34577120, Length=251, Percent_Identity=37.4501992031873, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI166795305, Length=147, Percent_Identity=42.1768707482993, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=52.3668639053254, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1787462, Length=341, Percent_Identity=36.3636363636364, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=201, Percent_Identity=33.8308457711443, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=310, Percent_Identity=32.5806451612903, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19921226, Length=220, Percent_Identity=40, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41527; Mature: 41527

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAETQANIEKIRNSLTLLGQRMDIETAPHRLEEFNARVEDPNLWNDPEKAQKLMRDRQV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LVDKMKQYETIRQGVDDNAELIELGEMEDDAEVVAEAEAALTALVDEAAAKELEALLDGE
HHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADSNDTFLEINSGAGGTESCDWASMLARMYTRWAEKQGYDVDLQSFSAGEEAGIKSAVYK
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHCHHHHEEE
ISGHNAYGWLKSESGVHRLVRISPFDSAAKRHTSFTSVKVYPVVDDNIEIEVNPSDIRID
ECCCCEEEEEECCCCCEEEEEECCCCHHHHHCCCCCEEEEEEEECCCEEEEECCCCEEEE
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQMELDKR
EEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SAKVNEVHENAGDAGWGNQIRSYVLQPYQMVKDLRTAFETSDTQGVLDGNIDGLMASVLA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
MDVSGKSRAEARGDD
HCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAETQANIEKIRNSLTLLGQRMDIETAPHRLEEFNARVEDPNLWNDPEKAQKLMRDRQV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LVDKMKQYETIRQGVDDNAELIELGEMEDDAEVVAEAEAALTALVDEAAAKELEALLDGE
HHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADSNDTFLEINSGAGGTESCDWASMLARMYTRWAEKQGYDVDLQSFSAGEEAGIKSAVYK
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHCHHHHEEE
ISGHNAYGWLKSESGVHRLVRISPFDSAAKRHTSFTSVKVYPVVDDNIEIEVNPSDIRID
ECCCCEEEEEECCCCCEEEEEECCCCHHHHHCCCCCEEEEEEEECCCEEEEECCCCEEEE
TYRSSGAGGQHVNTTDSAVRITHHPTGIVVTSSEKSQHQNRDIAMKALKSRLYQMELDKR
EEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SAKVNEVHENAGDAGWGNQIRSYVLQPYQMVKDLRTAFETSDTQGVLDGNIDGLMASVLA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
MDVSGKSRAEARGDD
HCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA