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The map label for this gene is 89055023

Identifier: 89055023

GI number: 89055023

Start: 2531281

End: 2532672

Strand: Reverse

Name: 89055023

Synonym: Jann_2532

Alternate gene names: NA

Gene position: 2532672-2531281 (Counterclockwise)

Preceding gene: 89055024

Following gene: 89055022

Centisome position: 58.65

GC content: 67.82

Gene sequence:

>1392_bases
ATGAAGGAGGGAGCAGCCGTGGCGCTTGACCTCTGGCGGCTGACGGCAACGGAGCTGTCGCGAAAGATCGCCGCGCGGGA
GGTGTCTTGCGTCGAGTTGATGCGCGCGACGCTTGCCCGGATCGATGCGGTGAACGGCGCGGTCAACGCTATCGTCTCCC
TGCGCGACCGGGAGGCGTTGATGGGAGAGGCGCGGGTGGCCGATGCCGTGCGCGGCGACGGCTGGCTTCACGGCATTCCG
GTGGCGATCAAGGATCTGGTGGCGCTGAAGGGGCTGCGCTCCACCTGGGGGTCGCGGATCCTCGCGGAGTTTGTGCCAGA
AGCGGACGACGCCTTGGCTCGTGCCTTGCGCGGCGCGGGCGCGATTGTGATCGGCAAAACCAATGTGCCGGAATACGGCT
TGGGGTCCCATTCCACAAACGGTGTGTTTGGGGTCACGCGCAATCCGTTCGATCTGTCGCGCACCGCTGGCGGGTCATCG
GGCGGGGCCGGGGCAGCCCTTGCCACCGGCATGGTGAGCGTCGCGGATGGCTCGGACATGATGGGCAGCCTGCGCAATCC
GGCGGCCTGGAATGACGTCTATGGGTTTCGGCCCACCGTCGGATTGGTTCCGGGAGAGCCGCGTGACAACGTGCTGTTGC
ACCGCCTGGCGACGCTGGGGCCCATGGGGCGCAGTATCGAGGACGTGACGGCGTTGCTGGCTACGCTCTCTGGGCAGAGG
ATCGACATACCCGAACCGCCAAAGTCGCCTCGGGTGGCGTGGCTTGGCGATTGGGGCGGCGCGTATCTTATGGATGACGG
GCTTCTGGACGCGGGCGCGCAGGCGTTGGGGCAGGCGGAGGCGCTCGGTTGGCAGGTTGAGGCCGTCAAGCCGCCCATGC
CAGCAGAAGACCTGTGGCAAAGTTGGACCGTGCTGCGGTCCTTCGTCGTCGCGCAAGACCTGGCCCGCCACTGGCGTGAC
CCGGATGCGCGCAAGGTGTTGAACGCGCAAGCCATTTGGGAGATCGAGCAGGGCCTCGCCCTGACCGGCGACGTAGTGGA
GCGGGCCGCCGCAATCCGCCGCGATTGGCTAGCAGTGTTGGCGGAACTGTTCAGGCGCTTAGATGCGCTCGTGCTGCCTG
CCACGCAAATGTGGCCGTTCCCGGCGGAGTGGGATTGGCCCCGCGCGATCTCTGGCACATCCACGGACACCTACCACCGA
TGGATGGAATGCGTGGTGCCCGCCAGCCTCGCGGGCGTGCCCGCTTTGGCGGTTCCCGGCGGCTACGGGGCGACGGGTCT
GCCCCACGGCCTGCAAATTATCGGCCCGCCGGGCGCGGATGCGAAGATCCTCGCCTTGGGGGCGGCGTGGGAGGCGATGG
TCGGCCCCCGAACGGTGCGCGATCCCACCTGA

Upstream 100 bases:

>100_bases
GGGCCGAGGCCCGGGGTGATGACTGAGGCGGGGCCGTGGGGCCGCTTCATCGTCCATTGCGCACGGGGCGGGGACCGGAA
TTTGAGTTGTATTTGCCCAG

Downstream 100 bases:

>100_bases
GCGACAGGCTTTGCACCACCGCATCCCCTTCGCTACACTGGCTGCGGGAGGATTTGCCATGTCTGACATCGCGACACACC
CTGTTGGAGAGGGCGCGCCC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKEGAAVALDLWRLTATELSRKIAAREVSCVELMRATLARIDAVNGAVNAIVSLRDREALMGEARVADAVRGDGWLHGIP
VAIKDLVALKGLRSTWGSRILAEFVPEADDALARALRGAGAIVIGKTNVPEYGLGSHSTNGVFGVTRNPFDLSRTAGGSS
GGAGAALATGMVSVADGSDMMGSLRNPAAWNDVYGFRPTVGLVPGEPRDNVLLHRLATLGPMGRSIEDVTALLATLSGQR
IDIPEPPKSPRVAWLGDWGGAYLMDDGLLDAGAQALGQAEALGWQVEAVKPPMPAEDLWQSWTVLRSFVVAQDLARHWRD
PDARKVLNAQAIWEIEQGLALTGDVVERAAAIRRDWLAVLAELFRRLDALVLPATQMWPFPAEWDWPRAISGTSTDTYHR
WMECVVPASLAGVPALAVPGGYGATGLPHGLQIIGPPGADAKILALGAAWEAMVGPRTVRDPT

Sequences:

>Translated_463_residues
MKEGAAVALDLWRLTATELSRKIAAREVSCVELMRATLARIDAVNGAVNAIVSLRDREALMGEARVADAVRGDGWLHGIP
VAIKDLVALKGLRSTWGSRILAEFVPEADDALARALRGAGAIVIGKTNVPEYGLGSHSTNGVFGVTRNPFDLSRTAGGSS
GGAGAALATGMVSVADGSDMMGSLRNPAAWNDVYGFRPTVGLVPGEPRDNVLLHRLATLGPMGRSIEDVTALLATLSGQR
IDIPEPPKSPRVAWLGDWGGAYLMDDGLLDAGAQALGQAEALGWQVEAVKPPMPAEDLWQSWTVLRSFVVAQDLARHWRD
PDARKVLNAQAIWEIEQGLALTGDVVERAAAIRRDWLAVLAELFRRLDALVLPATQMWPFPAEWDWPRAISGTSTDTYHR
WMECVVPASLAGVPALAVPGGYGATGLPHGLQIIGPPGADAKILALGAAWEAMVGPRTVRDPT
>Mature_463_residues
MKEGAAVALDLWRLTATELSRKIAAREVSCVELMRATLARIDAVNGAVNAIVSLRDREALMGEARVADAVRGDGWLHGIP
VAIKDLVALKGLRSTWGSRILAEFVPEADDALARALRGAGAIVIGKTNVPEYGLGSHSTNGVFGVTRNPFDLSRTAGGSS
GGAGAALATGMVSVADGSDMMGSLRNPAAWNDVYGFRPTVGLVPGEPRDNVLLHRLATLGPMGRSIEDVTALLATLSGQR
IDIPEPPKSPRVAWLGDWGGAYLMDDGLLDAGAQALGQAEALGWQVEAVKPPMPAEDLWQSWTVLRSFVVAQDLARHWRD
PDARKVLNAQAIWEIEQGLALTGDVVERAAAIRRDWLAVLAELFRRLDALVLPATQMWPFPAEWDWPRAISGTSTDTYHR
WMECVVPASLAGVPALAVPGGYGATGLPHGLQIIGPPGADAKILALGAAWEAMVGPRTVRDPT

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=25.2049180327869, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=26.2135922330097, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI166795287, Length=212, Percent_Identity=31.1320754716981, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=26.4112903225806, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17538252, Length=523, Percent_Identity=25.2390057361377, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=30.0970873786408, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=25, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17538254, Length=279, Percent_Identity=27.9569892473118, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=226, Percent_Identity=30.5309734513274, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=196, Percent_Identity=36.2244897959184, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=196, Percent_Identity=36.2244897959184, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=242, Percent_Identity=28.9256198347107, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319685, Length=156, Percent_Identity=35.2564102564103, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=24.8826291079812, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=26.9306930693069, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648113, Length=432, Percent_Identity=28.7037037037037, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24644968, Length=501, Percent_Identity=25.3493013972056, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652985, Length=245, Percent_Identity=33.8775510204082, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19922090, Length=245, Percent_Identity=33.8775510204082, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24652981, Length=245, Percent_Identity=33.8775510204082, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24652983, Length=245, Percent_Identity=33.8775510204082, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24648435, Length=242, Percent_Identity=33.4710743801653, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648437, Length=242, Percent_Identity=33.4710743801653, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45550774, Length=242, Percent_Identity=33.4710743801653, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356731, Length=239, Percent_Identity=30.1255230125523, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=34.3373493975904, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=34.3373493975904, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 49170; Mature: 49170

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEGAAVALDLWRLTATELSRKIAAREVSCVELMRATLARIDAVNGAVNAIVSLRDREAL
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGEARVADAVRGDGWLHGIPVAIKDLVALKGLRSTWGSRILAEFVPEADDALARALRGAG
HHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
AIVIGKTNVPEYGLGSHSTNGVFGVTRNPFDLSRTAGGSSGGAGAALATGMVSVADGSDM
EEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHCCCCCCCCCHHHHHHCCEEECCCHHH
MGSLRNPAAWNDVYGFRPTVGLVPGEPRDNVLLHRLATLGPMGRSIEDVTALLATLSGQR
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
IDIPEPPKSPRVAWLGDWGGAYLMDDGLLDAGAQALGQAEALGWQVEAVKPPMPAEDLWQ
CCCCCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHH
SWTVLRSFVVAQDLARHWRDPDARKVLNAQAIWEIEQGLALTGDVVERAAAIRRDWLAVL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AELFRRLDALVLPATQMWPFPAEWDWPRAISGTSTDTYHRWMECVVPASLAGVPALAVPG
HHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
GYGATGLPHGLQIIGPPGADAKILALGAAWEAMVGPRTVRDPT
CCCCCCCCCCCEEECCCCCCCEEEEECCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKEGAAVALDLWRLTATELSRKIAAREVSCVELMRATLARIDAVNGAVNAIVSLRDREAL
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGEARVADAVRGDGWLHGIPVAIKDLVALKGLRSTWGSRILAEFVPEADDALARALRGAG
HHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
AIVIGKTNVPEYGLGSHSTNGVFGVTRNPFDLSRTAGGSSGGAGAALATGMVSVADGSDM
EEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHCCCCCCCCCHHHHHHCCEEECCCHHH
MGSLRNPAAWNDVYGFRPTVGLVPGEPRDNVLLHRLATLGPMGRSIEDVTALLATLSGQR
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
IDIPEPPKSPRVAWLGDWGGAYLMDDGLLDAGAQALGQAEALGWQVEAVKPPMPAEDLWQ
CCCCCCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHH
SWTVLRSFVVAQDLARHWRDPDARKVLNAQAIWEIEQGLALTGDVVERAAAIRRDWLAVL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AELFRRLDALVLPATQMWPFPAEWDWPRAISGTSTDTYHRWMECVVPASLAGVPALAVPG
HHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
GYGATGLPHGLQIIGPPGADAKILALGAAWEAMVGPRTVRDPT
CCCCCCCCCCCEEECCCCCCCEEEEECCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]