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The map label for this gene is gsiD [H]

Identifier: 89054956

GI number: 89054956

Start: 2459763

End: 2461235

Strand: Direct

Name: gsiD [H]

Synonym: Jann_2465

Alternate gene names: 89054956

Gene position: 2459763-2461235 (Clockwise)

Preceding gene: 89054955

Following gene: 89054957

Centisome position: 56.97

GC content: 62.05

Gene sequence:

>1473_bases
ATGGAACCTCTGACCTGGTCCGGCTCTTTCGGAGCCTTCCTCAACCCCCTTATGCTCGTGGCGCTGGCCATTTTCGTCCT
GGGCTTTCTTGCCAACACCGTTCTGACCATCGCAGGCGTGCGCGCCGGGGACCTGGAGTTCAACGGCGACGGCACCATGT
CGCTGCGCAAAACGCCGGTCGATTACGCGCTCGACACGATGAAATACGCGGTCCTGCTGTTTGCCCTGTGCTGTGTGGGC
TTCATCATCGGCGGCCTTGTCCTGCCCACGATCGAACAGAAGGGCATTCTTGGGGCCATGGCGCTGCGGCTGCTGCCCGT
TTGGATCGCCCTTATCGTGACCTTCGCGGTGTCGATCACCTTCAAGCGCAAACTTGGCCTCTACGGCAAGCTGTTCGACA
GCCCCATCGGCATGGCCGGGTTCATTCTGGTGATGTTCTGGATCTTCGCCGCGATCTTCTCGGGCGTATTCCCCCTGGTG
CCCACCCACGGGGCCATCGACCTGATTTCCTCCATGCGCAATGAGTTGCCGGGAACGCCGATCCCGGTCTCGGACGAGGG
CGAATATCCCTATTACCTTCTGGGCGGGGACAATCTGGGCCGCGACGTCTTCTCCCGCGTGTTCTTCGGGGCGGCGGACG
TCTTAAGCATCACGCCGGTGGCCACGCTCTTTGCCTTCATGGTCGGCATCACGCTCGGCCTGCCAGCGGGCTATTTCGGC
GGCAAGCTGGACACGATCCTGACCTTCATCGCCAATCTCGCGCTGGCCTTCCCCGTGATCCTGCTGTTCTTCCTGCTCGT
CACGCCCGAGATTATCGCGGCAGGCGTCCCGCAATACATGTCGATGGTGCTGTTCCTGTTTCCGATCCTGTTCCTGGTCG
TGCTGTTCAACTCGCGCTTCCACACGGATCCGGTGCGCCGGAACCTCTTTGTGGGGATCGCGGCGGCTTTGGGGTTGTGG
GCCTACCTCTCGCTCATCTCCAATGCGTCCGATGCCGATCTTCCGGCGCTCTTTCGCCTGTGGCCCGAAGCGCTGGACCT
CTTCGACATCGAAGGCAATCTGCTGATCGTCTTCGTGTCCGTGGTCTTCGTGAACTCGCCCACCGTGTTTCGCATCGTGC
GGGGCATCACGCTTGATGTGAAAACCCGCGACTATGTGGCCGCCGCCCAGACCCGCGGCGAAGGCCCGTGGTACATCATG
TTGTGGGAGATCCTGCCCAACGCGCGCGGGCCGCTGATCGTCGATTTCTGCCTGCGCATCGGCTACACGACGATCCTTCT
GGGCACATTGGGCTTCTTCGGCCTCGGCGTGTCCCCTGAATCCCCCGATTGGGGATCGACCATCAACGAAGGCCGTCGCC
TGCTGACGATCTACCCCCACCCGGCCCTGCCCCCCGCGCTGGCGCTGATGAGCCTCGTGCTTGGTCTGAACCTTCTGGCC
GACGGTTTGCGCGAAGAGAGCTTGAAGGATTGA

Upstream 100 bases:

>100_bases
ATCGTCGCGGTCTTCGTGGTGCTGCTGACCCAGCTGATCTCGGATATCGGCTACGTCTTCCTCAACCCCCGCATTCGTAT
CGCGTGAAGGAGCCTGATCC

Downstream 100 bases:

>100_bases
TGATGGTGCACCCGGCCCTCCTCGCGACGCTCTTCGCAGCCGCACCGTTCGGGCCAGCGGCCTGGGCCGACGACCTCGAC
CCAGACCTTCTGACCTGCAT

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MEPLTWSGSFGAFLNPLMLVALAIFVLGFLANTVLTIAGVRAGDLEFNGDGTMSLRKTPVDYALDTMKYAVLLFALCCVG
FIIGGLVLPTIEQKGILGAMALRLLPVWIALIVTFAVSITFKRKLGLYGKLFDSPIGMAGFILVMFWIFAAIFSGVFPLV
PTHGAIDLISSMRNELPGTPIPVSDEGEYPYYLLGGDNLGRDVFSRVFFGAADVLSITPVATLFAFMVGITLGLPAGYFG
GKLDTILTFIANLALAFPVILLFFLLVTPEIIAAGVPQYMSMVLFLFPILFLVVLFNSRFHTDPVRRNLFVGIAAALGLW
AYLSLISNASDADLPALFRLWPEALDLFDIEGNLLIVFVSVVFVNSPTVFRIVRGITLDVKTRDYVAAAQTRGEGPWYIM
LWEILPNARGPLIVDFCLRIGYTTILLGTLGFFGLGVSPESPDWGSTINEGRRLLTIYPHPALPPALALMSLVLGLNLLA
DGLREESLKD

Sequences:

>Translated_490_residues
MEPLTWSGSFGAFLNPLMLVALAIFVLGFLANTVLTIAGVRAGDLEFNGDGTMSLRKTPVDYALDTMKYAVLLFALCCVG
FIIGGLVLPTIEQKGILGAMALRLLPVWIALIVTFAVSITFKRKLGLYGKLFDSPIGMAGFILVMFWIFAAIFSGVFPLV
PTHGAIDLISSMRNELPGTPIPVSDEGEYPYYLLGGDNLGRDVFSRVFFGAADVLSITPVATLFAFMVGITLGLPAGYFG
GKLDTILTFIANLALAFPVILLFFLLVTPEIIAAGVPQYMSMVLFLFPILFLVVLFNSRFHTDPVRRNLFVGIAAALGLW
AYLSLISNASDADLPALFRLWPEALDLFDIEGNLLIVFVSVVFVNSPTVFRIVRGITLDVKTRDYVAAAQTRGEGPWYIM
LWEILPNARGPLIVDFCLRIGYTTILLGTLGFFGLGVSPESPDWGSTINEGRRLLTIYPHPALPPALALMSLVLGLNLLA
DGLREESLKD
>Mature_490_residues
MEPLTWSGSFGAFLNPLMLVALAIFVLGFLANTVLTIAGVRAGDLEFNGDGTMSLRKTPVDYALDTMKYAVLLFALCCVG
FIIGGLVLPTIEQKGILGAMALRLLPVWIALIVTFAVSITFKRKLGLYGKLFDSPIGMAGFILVMFWIFAAIFSGVFPLV
PTHGAIDLISSMRNELPGTPIPVSDEGEYPYYLLGGDNLGRDVFSRVFFGAADVLSITPVATLFAFMVGITLGLPAGYFG
GKLDTILTFIANLALAFPVILLFFLLVTPEIIAAGVPQYMSMVLFLFPILFLVVLFNSRFHTDPVRRNLFVGIAAALGLW
AYLSLISNASDADLPALFRLWPEALDLFDIEGNLLIVFVSVVFVNSPTVFRIVRGITLDVKTRDYVAAAQTRGEGPWYIM
LWEILPNARGPLIVDFCLRIGYTTILLGTLGFFGLGVSPESPDWGSTINEGRRLLTIYPHPALPPALALMSLVLGLNLLA
DGLREESLKD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=129, Percent_Identity=39.5348837209302, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787760, Length=358, Percent_Identity=25.6983240223464, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789964, Length=127, Percent_Identity=33.0708661417323, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787549, Length=119, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 53266; Mature: 53266

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00761 SPASE_I_3 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCC
>Mature Secondary Structure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HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA