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The map label for this gene is miaA

Identifier: 89054952

GI number: 89054952

Start: 2454720

End: 2455586

Strand: Direct

Name: miaA

Synonym: Jann_2461

Alternate gene names: 89054952

Gene position: 2454720-2455586 (Clockwise)

Preceding gene: 89054938

Following gene: 89054953

Centisome position: 56.85

GC content: 68.63

Gene sequence:

>867_bases
TTGATAAACATCAACAACATTAACCCAGAGAAGCCGGTTCTGATCGTGGGCCCCACGGCCTCCGGCAAAACGTCGCTGGC
CATTGCCATCGCGCAGGCCCAGGGCCGTGTTGTGGTCAACGCCGATGCGTTGCAGGTCTATGACGGCTGGCGCGTGCTGA
CGGCCCGTCCCACGCCGGAGGAGGAGGACGCCGTCCCCCATCACCTCTACGGCCATGTGCCCTTTACCGCCCATTACGAC
GTCGGCCAATGGTTGCAGGATCTGACCCCCTTCCTTGCGCAGAACCCGGTCATCGTCGGTGGCACCGGCCTTTACTTTCG
GGCCCTCACGCAGGGCCTCGCCGACATCCCCGCTATCCCGCCGGACATCCGCGCCGAGGCGGACGCGCGCTCCCACACCC
AGCGCCTGGCTGACCTCCACGAAGGCGACCCCGCGCTTGTCGCCCGCATCGATTGCGACAACCCCATGCGCGTGCAGCGC
GGGTGGGAGGTGCTGCGCGCCACCGGGCGGCCCCTGTCGGCCTGGCAGGATGAGACACCGCCCCCGCTCCTGCCCCTCGG
CAACACCACCCCCCTCGCCCTGATGCCGGACCGCGACTGGCTCAACGCCCGCATCGCACAGCGCTTTGACCAGATGCTCA
ACGAAGGTGCTTTGGATGAAGCGCGCGCCAACCTCCCCCGCTGGTCCAGCGCGGGCGGGGCCGCGAAGGCCATCGGCGCG
CCCGAGCTTATCGCCCACCTACACGGAACCCTGCCCCTTGCCGCCGCGCGCGACGCCGCCGTCACGGCCACCCGCCAATA
CGCCAAGCGCCAACGCAGCTGGCAACGCAGCAATACCCAGGCCTGGCAGAGCGTCCCTCTGCCATAG

Upstream 100 bases:

>100_bases
CCTTGTTTGATCTTGCGGCGCGAGAATGGTCTAAATGGCGCGCCGGTTCAACGCCCCATCCGCCACACTCCGCGCAACTT
GAAGAACGACCTACAAAACA

Downstream 100 bases:

>100_bases
GGGCTCCTGTTTCATCTGGGCCGGTACAAGTCCGGGGGGAATGCGATCTTTTTCCTCGAAAAAAGACGCAGGGGGGCAGC
GCCCCCTTTGCGACATCGAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MININNINPEKPVLIVGPTASGKTSLAIAIAQAQGRVVVNADALQVYDGWRVLTARPTPEEEDAVPHHLYGHVPFTAHYD
VGQWLQDLTPFLAQNPVIVGGTGLYFRALTQGLADIPAIPPDIRAEADARSHTQRLADLHEGDPALVARIDCDNPMRVQR
GWEVLRATGRPLSAWQDETPPPLLPLGNTTPLALMPDRDWLNARIAQRFDQMLNEGALDEARANLPRWSSAGGAAKAIGA
PELIAHLHGTLPLAAARDAAVTATRQYAKRQRSWQRSNTQAWQSVPLP

Sequences:

>Translated_288_residues
MININNINPEKPVLIVGPTASGKTSLAIAIAQAQGRVVVNADALQVYDGWRVLTARPTPEEEDAVPHHLYGHVPFTAHYD
VGQWLQDLTPFLAQNPVIVGGTGLYFRALTQGLADIPAIPPDIRAEADARSHTQRLADLHEGDPALVARIDCDNPMRVQR
GWEVLRATGRPLSAWQDETPPPLLPLGNTTPLALMPDRDWLNARIAQRFDQMLNEGALDEARANLPRWSSAGGAAKAIGA
PELIAHLHGTLPLAAARDAAVTATRQYAKRQRSWQRSNTQAWQSVPLP
>Mature_288_residues
MININNINPEKPVLIVGPTASGKTSLAIAIAQAQGRVVVNADALQVYDGWRVLTARPTPEEEDAVPHHLYGHVPFTAHYD
VGQWLQDLTPFLAQNPVIVGGTGLYFRALTQGLADIPAIPPDIRAEADARSHTQRLADLHEGDPALVARIDCDNPMRVQR
GWEVLRATGRPLSAWQDETPPPLLPLGNTTPLALMPDRDWLNARIAQRFDQMLNEGALDEARANLPRWSSAGGAAKAIGA
PELIAHLHGTLPLAAARDAAVTATRQYAKRQRSWQRSNTQAWQSVPLP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=24.9190938511327, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790613, Length=298, Percent_Identity=32.5503355704698, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=23.1788079470199, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=28.7313432835821, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_JANSC (Q28PI4)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510403.1
- ProteinModelPortal:   Q28PI4
- SMR:   Q28PI4
- STRING:   Q28PI4
- GeneID:   3934918
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2461
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   JSP290400:JANN_2461-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 31387; Mature: 31387

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MININNINPEKPVLIVGPTASGKTSLAIAIAQAQGRVVVNADALQVYDGWRVLTARPTPE
CEECCCCCCCCCEEEECCCCCCCCEEEEEEEECCCEEEEECCEEEEECCEEEEEECCCCC
EEDAVPHHLYGHVPFTAHYDVGQWLQDLTPFLAQNPVIVGGTGLYFRALTQGLADIPAIP
CCCCCCCCEECCCCEEEECCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCC
PDIRAEADARSHTQRLADLHEGDPALVARIDCDNPMRVQRGWEVLRATGRPLSAWQDETP
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
PPLLPLGNTTPLALMPDRDWLNARIAQRFDQMLNEGALDEARANLPRWSSAGGAAKAIGA
CCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHCCH
PELIAHLHGTLPLAAARDAAVTATRQYAKRQRSWQRSNTQAWQSVPLP
HHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure
MININNINPEKPVLIVGPTASGKTSLAIAIAQAQGRVVVNADALQVYDGWRVLTARPTPE
CEECCCCCCCCCEEEECCCCCCCCEEEEEEEECCCEEEEECCEEEEECCEEEEEECCCCC
EEDAVPHHLYGHVPFTAHYDVGQWLQDLTPFLAQNPVIVGGTGLYFRALTQGLADIPAIP
CCCCCCCCEECCCCEEEECCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCC
PDIRAEADARSHTQRLADLHEGDPALVARIDCDNPMRVQRGWEVLRATGRPLSAWQDETP
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
PPLLPLGNTTPLALMPDRDWLNARIAQRFDQMLNEGALDEARANLPRWSSAGGAAKAIGA
CCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHCCH
PELIAHLHGTLPLAAARDAAVTATRQYAKRQRSWQRSNTQAWQSVPLP
HHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA