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The map label for this gene is potB [H]

Identifier: 89054893

GI number: 89054893

Start: 2402591

End: 2403679

Strand: Reverse

Name: potB [H]

Synonym: Jann_2402

Alternate gene names: 89054893

Gene position: 2403679-2402591 (Counterclockwise)

Preceding gene: 89054894

Following gene: 89054892

Centisome position: 55.67

GC content: 62.26

Gene sequence:

>1089_bases
GTGGCGGGAACAAAGGACAGGTGCTTGGGGCAGGGGCTATCACAGGAGGCGAAGCGGGGGCTGATCACGGTCAGTCCGCC
GCTGTTATTCGCGCTGCTGCTGCTGGCGGTGCCGCTTCTGACGATTATCCTGTTCAGCTTCTGGACGCAGAACTTCCTGG
AGATCGACCGGACGTTCACGCTTGAGAATTACCGCGAGGCGCTGACCGATCCGCTTTATCGCGCGCTGATGTTGCGGAGC
CTGCGGATCTCGGCCTCCGTCACCGCCGTCACGGTCGTGCTGGCCTTCCCGATCGCCTATTATATCAGCTTCCATGTGCG
CCCGGAAAAGAAGGCGCTGTGGCTGTTTCTGATTACCATTCCGTTCTGGACCAGCTACCTGATCCGCATCTTCCTGTGGC
GGGTGATCCTGGCCTATGACGGGCCGATCAACGGCACGCTTCTGGGCTTGGGCGTGATCGAGGAGCCGTTGTCGTTTATC
CTCTACAATGCCAATGCGGTCGTCATCACGCTTGCCCATGCCTTCGCGCCCTTCGCGATCCTGCCGATTTTCGTGAGCCT
TGAGAAGATCGATCGGTCCCTGCTGGAGGCGGCGCGGGATCTTGGCGAGGCGACGGTGATGACCTTTGCGCGCGTCACCC
TGCCGCTGGCGATGCCGGGGGTGCTGGCGGCTGTCCTGATCGTGTTCATCCCGACGGTGGGCGATTACGTCACGCCGCGG
CTGGTGGGCGGGTCCGGCGGGACGATGATCGCCAACATGATCTATGTGCAGATCTTTGACCTCTCGAACCGGCCCATGGG
CGCGACTTTGGCCGTCTTTGCAATGGCTTGCGTGACGATCACCCTGGCCTGGGGAATTATGCATATCCGCTTCTGGGTGC
ATCTGAATGACCGCTATCGCGGGGGCGCGCGCGGATTGGCGGTGGCGGGCGTGGCTGCCGTTGTCGACATCGGCATCCTG
TGGTGGCTTTTGGTCAACGGCGACACCTATTTCGGGCCCGCAGGCACCCTTGGCCTGATGGCGGCGATCATGGCGGGGGG
CGCGATTGCCATCGCGGTCTTTGTGCCAAAGGGCAGGTGGGGGATATGA

Upstream 100 bases:

>100_bases
CGTGGCAGAAAATTTGAACGTCCGGCAAGCATCGAAAGGCACGCCCTCGCCTTGCTCTCCATCCCCCCTTGCGGTGGCCC
CGTGATCCGGTGATTGTCGG

Downstream 100 bases:

>100_bases
GGTTTTCAGCCCTTGGCATCTTCGCCGTGCTCTACCTGATCTTCCTTTATGCACCGATCATCCTGCTGCCGATTTTCGCG
TTCAACGACGGCACAATTAT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAGTKDRCLGQGLSQEAKRGLITVSPPLLFALLLLAVPLLTIILFSFWTQNFLEIDRTFTLENYREALTDPLYRALMLRS
LRISASVTAVTVVLAFPIAYYISFHVRPEKKALWLFLITIPFWTSYLIRIFLWRVILAYDGPINGTLLGLGVIEEPLSFI
LYNANAVVITLAHAFAPFAILPIFVSLEKIDRSLLEAARDLGEATVMTFARVTLPLAMPGVLAAVLIVFIPTVGDYVTPR
LVGGSGGTMIANMIYVQIFDLSNRPMGATLAVFAMACVTITLAWGIMHIRFWVHLNDRYRGGARGLAVAGVAAVVDIGIL
WWLLVNGDTYFGPAGTLGLMAAIMAGGAIAIAVFVPKGRWGI

Sequences:

>Translated_362_residues
MAGTKDRCLGQGLSQEAKRGLITVSPPLLFALLLLAVPLLTIILFSFWTQNFLEIDRTFTLENYREALTDPLYRALMLRS
LRISASVTAVTVVLAFPIAYYISFHVRPEKKALWLFLITIPFWTSYLIRIFLWRVILAYDGPINGTLLGLGVIEEPLSFI
LYNANAVVITLAHAFAPFAILPIFVSLEKIDRSLLEAARDLGEATVMTFARVTLPLAMPGVLAAVLIVFIPTVGDYVTPR
LVGGSGGTMIANMIYVQIFDLSNRPMGATLAVFAMACVTITLAWGIMHIRFWVHLNDRYRGGARGLAVAGVAAVVDIGIL
WWLLVNGDTYFGPAGTLGLMAAIMAGGAIAIAVFVPKGRWGI
>Mature_361_residues
AGTKDRCLGQGLSQEAKRGLITVSPPLLFALLLLAVPLLTIILFSFWTQNFLEIDRTFTLENYREALTDPLYRALMLRSL
RISASVTAVTVVLAFPIAYYISFHVRPEKKALWLFLITIPFWTSYLIRIFLWRVILAYDGPINGTLLGLGVIEEPLSFIL
YNANAVVITLAHAFAPFAILPIFVSLEKIDRSLLEAARDLGEATVMTFARVTLPLAMPGVLAAVLIVFIPTVGDYVTPRL
VGGSGGTMIANMIYVQIFDLSNRPMGATLAVFAMACVTITLAWGIMHIRFWVHLNDRYRGGARGLAVAGVAAVVDIGILW
WLLVNGDTYFGPAGTLGLMAAIMAGGAIAIAVFVPKGRWGI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=264, Percent_Identity=38.2575757575758, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1787080, Length=235, Percent_Identity=36.1702127659575, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787713, Length=259, Percent_Identity=33.5907335907336, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 39534; Mature: 39403

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTKDRCLGQGLSQEAKRGLITVSPPLLFALLLLAVPLLTIILFSFWTQNFLEIDRTFT
CCCCCCHHHHCCCCHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LENYREALTDPLYRALMLRSLRISASVTAVTVVLAFPIAYYISFHVRPEKKALWLFLITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEHHH
PFWTSYLIRIFLWRVILAYDGPINGTLLGLGVIEEPLSFILYNANAVVITLAHAFAPFAI
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHEEECCCEEEEEHHHHHHHHHH
LPIFVSLEKIDRSLLEAARDLGEATVMTFARVTLPLAMPGVLAAVLIVFIPTVGDYVTPR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCE
LVGGSGGTMIANMIYVQIFDLSNRPMGATLAVFAMACVTITLAWGIMHIRFWVHLNDRYR
EECCCCCHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
GGARGLAVAGVAAVVDIGILWWLLVNGDTYFGPAGTLGLMAAIMAGGAIAIAVFVPKGRW
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCC
GI
CC
>Mature Secondary Structure 
AGTKDRCLGQGLSQEAKRGLITVSPPLLFALLLLAVPLLTIILFSFWTQNFLEIDRTFT
CCCCCHHHHCCCCHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LENYREALTDPLYRALMLRSLRISASVTAVTVVLAFPIAYYISFHVRPEKKALWLFLITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEHHH
PFWTSYLIRIFLWRVILAYDGPINGTLLGLGVIEEPLSFILYNANAVVITLAHAFAPFAI
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHEEECCCEEEEEHHHHHHHHHH
LPIFVSLEKIDRSLLEAARDLGEATVMTFARVTLPLAMPGVLAAVLIVFIPTVGDYVTPR
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCE
LVGGSGGTMIANMIYVQIFDLSNRPMGATLAVFAMACVTITLAWGIMHIRFWVHLNDRYR
EECCCCCHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCC
GGARGLAVAGVAAVVDIGILWWLLVNGDTYFGPAGTLGLMAAIMAGGAIAIAVFVPKGRW
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCC
GI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]