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The map label for this gene is phoB [H]

Identifier: 89054785

GI number: 89054785

Start: 2300776

End: 2301462

Strand: Direct

Name: phoB [H]

Synonym: Jann_2294

Alternate gene names: 89054785

Gene position: 2300776-2301462 (Clockwise)

Preceding gene: 89054784

Following gene: 89054790

Centisome position: 53.28

GC content: 63.76

Gene sequence:

>687_bases
ATGGCCGGTGAACCCCTTGTTCTGGTGGTGGAGGATGAGCCCGCGCAGCGCGAGGTTTTGGCCTATAACATCCGGGCCGA
GGGCTTTGACGTCGTGACCGCCGCGGCGGGCGATGAGGCGCTGGTAGCGGTGCGCGAAACACCCCCCGATGTGATCGTGC
TGGACTGGATGCTGCCCCATGTCTCAGGGATTGAGGTTTGCCGCCAACTGAAGATGGGGTCCGAGACCAAGAAGATCCCG
GTCATCATGCTGTCGGCGCGGTCCGAGGACACGGACAAGGTGAGGGGTCTTGAGACCGGCGCGGATGACTACATCACCAA
GCCGTATTCCGTGGCCGAATTGCTCGCCCGCCTGCGCACGCAGTTGCGCCGTGTGCGCCCCTCCACTGTCGCGGAGCGTT
TGGAATATGAAGACATCGCCATCGACCTGACCGAACACCGCGTCTATCGCGGCGGCGATACGCTGTCCCTTGGCCCTACA
GAGTTTCGCCTGCTTAGCGCGTTCATGGAACGTCCGGGCCGCGTGTGGTCGCGGGAGCAATTGCTGGATCGGGTCTGGGG
CCGGGACATCTACGTCGATAGCCGCACGGTCGATGTCCATGTGGGTCGCCTGCGCAAGGCCCTGAAGGTTGGGGAGGCCG
GTGATCCCATCCGCACCGTGCGCGGGGCTGGCTACGCTTTAGGTTAA

Upstream 100 bases:

>100_bases
CTCCGTGGCCGAACAGGTCATATATCTGGTGACCGGCGAGATGCCGGACGAGGACCGCCCGAAAGAAGACAAGACCCCCT
ACGTGCAAGGGGCCTGACCC

Downstream 100 bases:

>100_bases
CGTCAGTCCGTAAGTCTTTGCTGCAGAATTGAAAAACACATCCTGATGCGCGTCCTCAGGCACCATGTCCAGATGGACAT
CGCGGCAGGTCTCATACCGC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MAGEPLVLVVEDEPAQREVLAYNIRAEGFDVVTAAAGDEALVAVRETPPDVIVLDWMLPHVSGIEVCRQLKMGSETKKIP
VIMLSARSEDTDKVRGLETGADDYITKPYSVAELLARLRTQLRRVRPSTVAERLEYEDIAIDLTEHRVYRGGDTLSLGPT
EFRLLSAFMERPGRVWSREQLLDRVWGRDIYVDSRTVDVHVGRLRKALKVGEAGDPIRTVRGAGYALG

Sequences:

>Translated_228_residues
MAGEPLVLVVEDEPAQREVLAYNIRAEGFDVVTAAAGDEALVAVRETPPDVIVLDWMLPHVSGIEVCRQLKMGSETKKIP
VIMLSARSEDTDKVRGLETGADDYITKPYSVAELLARLRTQLRRVRPSTVAERLEYEDIAIDLTEHRVYRGGDTLSLGPT
EFRLLSAFMERPGRVWSREQLLDRVWGRDIYVDSRTVDVHVGRLRKALKVGEAGDPIRTVRGAGYALG
>Mature_227_residues
AGEPLVLVVEDEPAQREVLAYNIRAEGFDVVTAAAGDEALVAVRETPPDVIVLDWMLPHVSGIEVCRQLKMGSETKKIPV
IMLSARSEDTDKVRGLETGADDYITKPYSVAELLARLRTQLRRVRPSTVAERLEYEDIAIDLTEHRVYRGGDTLSLGPTE
FRLLSAFMERPGRVWSREQLLDRVWGRDIYVDSRTVDVHVGRLRKALKVGEAGDPIRTVRGAGYALG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=52.5114155251142, Blast_Score=230, Evalue=5e-62,
Organism=Escherichia coli, GI1788394, Length=223, Percent_Identity=41.2556053811659, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=41.7777777777778, Blast_Score=153, Evalue=8e-39,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=39.1111111111111, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=37.10407239819, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=33.9130434782609, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=33.0316742081448, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=32.8947368421053, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=32.7510917030568, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=35.3711790393013, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=35.7142857142857, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=31.0502283105023, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=30.6034482758621, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=32.4074074074074, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=32.7586206896552, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25425; Mature: 25294

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGEPLVLVVEDEPAQREVLAYNIRAEGFDVVTAAAGDEALVAVRETPPDVIVLDWMLPH
CCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCEEEEEECCCCCEEEEECCCCC
VSGIEVCRQLKMGSETKKIPVIMLSARSEDTDKVRGLETGADDYITKPYSVAELLARLRT
CHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHH
QLRRVRPSTVAERLEYEDIAIDLTEHRVYRGGDTLSLGPTEFRLLSAFMERPGRVWSREQ
HHHHCCHHHHHHHHCHHHEEEEHHHCEEECCCCEEEECHHHHHHHHHHHHCCCCCCCHHH
LLDRVWGRDIYVDSRTVDVHVGRLRKALKVGEAGDPIRTVRGAGYALG
HHHHHCCCEEEECCEEEEEEHHHHHHHHHCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
AGEPLVLVVEDEPAQREVLAYNIRAEGFDVVTAAAGDEALVAVRETPPDVIVLDWMLPH
CCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCEEEEEECCCCCEEEEECCCCC
VSGIEVCRQLKMGSETKKIPVIMLSARSEDTDKVRGLETGADDYITKPYSVAELLARLRT
CHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCHHCCCCHHHHHHHHHHHH
QLRRVRPSTVAERLEYEDIAIDLTEHRVYRGGDTLSLGPTEFRLLSAFMERPGRVWSREQ
HHHHCCHHHHHHHHCHHHEEEEHHHCEEECCCCEEEECHHHHHHHHHHHHCCCCCCCHHH
LLDRVWGRDIYVDSRTVDVHVGRLRKALKVGEAGDPIRTVRGAGYALG
HHHHHCCCEEEECCEEEEEEHHHHHHHHHCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]