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The map label for this gene is proA

Identifier: 89054776

GI number: 89054776

Start: 2290934

End: 2292202

Strand: Direct

Name: proA

Synonym: Jann_2285

Alternate gene names: 89054776

Gene position: 2290934-2292202 (Clockwise)

Preceding gene: 89054775

Following gene: 89054777

Centisome position: 53.06

GC content: 63.59

Gene sequence:

>1269_bases
ATGAAAGACCTGACCGATAACGTCCCCGCCCTGATGGCCGAAATCGGCGCGCGCGCCAAAAATGCCAGCGCAGAACTGGC
ATTTGCCTCGCCAGAGGCCAAGTTTACGGCGCTGACGGCGGCGGCGGATGCCGTCTGGGCGCGCCGGGCTGACATCATCG
CGGCGAATGAGAAAGACATGGCGTTCGGGCGCGAGAAGGGGCTTTCGGACGCGATGCTGGACCGGCTGATGCTGGATGAG
GCCCGGGTGGAGGGGATCGTGACAGGCTTGCGCGCGGTCGCAGGGCAGGAGGATCCCGTGGGTCACGTGATGACCGAATG
GGAGATGGAGAGCGGGCTGCACATCAAGCGCGTTCGCACGCCCCTCGGCGTGATCGGTGTGATCTATGAGAGCCGCCCCA
ATGTAACGGCGGATGCGGGCGCGTTGTGCCTGAAATCCGGCAACGCGGTGATCTTGCGGGGCGGATCGGAAAGCTTCCAT
TCCTCGGGCGCGCTGGCGGATTGTCTGCAAGAGGGGCTGCGGACGGCGGGCCTGCCGGAAGATGCCGTCCAGCGCGTCCC
CACCCGCGACCGCGCCGCCGTGTCCGAGATGTTGGCGATGACCGAGACCATCGACGTGATCGTGCCGCGCGGCGGCAAGG
GCCTTGTGGCGCTGGTGCAGCGAGAGGCGCGGGTGCCCGTCTTTGCGCATCTGGAGGGGATTTGCCACATCTACCTCGAC
GCCTCCGCCGATCCGGAAAAGGCGCTGCGTGTGGTCTTGAACGCCAAGACCCGCCGGACCGGGATATGTGGCTCGGCCGA
GTGTCTGCTGATCCACCAAGACATCGCCGACACCATTGGTCAGGGTGTTGTACGGGCTTTAATTGATGCAGGCGTAGAGG
TCCGCGGCGATAAAAGTATTGAGGAAATTGAAGGGGTTACGCGCGCAAGTGAAAGTGATTGGGGCCAGGAATTCCTCGAC
ATGATCATCGCCGCGCGGGTTGTCGAGGATCTGGACGACGCGCTTGATCACATCCGCACCTATGGCTCCAACCATACCGA
CGCAATCATTGCCGAGGACGACGCGGCCGTCGCGCGGTTCTTCCAGCGGCTCGACAGCGCGATCCTGATACACAACGCCT
CGACCCAGTTTGCCGATGGCGGGGAGTTTGGGATGGGCGCGGAGATCGGGATTGCGACGGGCAAGATGCATGCACGGGGT
CCGGTGGGGTCAGAGCAGTTGACCTCGTTCAAATATCTGGTGACGGGCGATGGGACAATTCGCGGCTGA

Upstream 100 bases:

>100_bases
GCGCCGCCCTGGTGCACCGTGACGATATGGTGCTCTAGCCCCTTCACAGACGCTCAAAACCCAATATCCTGTCCCCTCCG
CCCCTGCATCGAGGTGTCCC

Downstream 100 bases:

>100_bases
CGGCGCGTTGGACGGGTTGCGGTAGTGGGGGCCTGCCCCCACACCTCCGGGATATTTATGGAACGAGGAAGAGGAGGGGG
CCTGCGATGAGCGATGGGCG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKDLTDNVPALMAEIGARAKNASAELAFASPEAKFTALTAAADAVWARRADIIAANEKDMAFGREKGLSDAMLDRLMLDE
ARVEGIVTGLRAVAGQEDPVGHVMTEWEMESGLHIKRVRTPLGVIGVIYESRPNVTADAGALCLKSGNAVILRGGSESFH
SSGALADCLQEGLRTAGLPEDAVQRVPTRDRAAVSEMLAMTETIDVIVPRGGKGLVALVQREARVPVFAHLEGICHIYLD
ASADPEKALRVVLNAKTRRTGICGSAECLLIHQDIADTIGQGVVRALIDAGVEVRGDKSIEEIEGVTRASESDWGQEFLD
MIIAARVVEDLDDALDHIRTYGSNHTDAIIAEDDAAVARFFQRLDSAILIHNASTQFADGGEFGMGAEIGIATGKMHARG
PVGSEQLTSFKYLVTGDGTIRG

Sequences:

>Translated_422_residues
MKDLTDNVPALMAEIGARAKNASAELAFASPEAKFTALTAAADAVWARRADIIAANEKDMAFGREKGLSDAMLDRLMLDE
ARVEGIVTGLRAVAGQEDPVGHVMTEWEMESGLHIKRVRTPLGVIGVIYESRPNVTADAGALCLKSGNAVILRGGSESFH
SSGALADCLQEGLRTAGLPEDAVQRVPTRDRAAVSEMLAMTETIDVIVPRGGKGLVALVQREARVPVFAHLEGICHIYLD
ASADPEKALRVVLNAKTRRTGICGSAECLLIHQDIADTIGQGVVRALIDAGVEVRGDKSIEEIEGVTRASESDWGQEFLD
MIIAARVVEDLDDALDHIRTYGSNHTDAIIAEDDAAVARFFQRLDSAILIHNASTQFADGGEFGMGAEIGIATGKMHARG
PVGSEQLTSFKYLVTGDGTIRG
>Mature_422_residues
MKDLTDNVPALMAEIGARAKNASAELAFASPEAKFTALTAAADAVWARRADIIAANEKDMAFGREKGLSDAMLDRLMLDE
ARVEGIVTGLRAVAGQEDPVGHVMTEWEMESGLHIKRVRTPLGVIGVIYESRPNVTADAGALCLKSGNAVILRGGSESFH
SSGALADCLQEGLRTAGLPEDAVQRVPTRDRAAVSEMLAMTETIDVIVPRGGKGLVALVQREARVPVFAHLEGICHIYLD
ASADPEKALRVVLNAKTRRTGICGSAECLLIHQDIADTIGQGVVRALIDAGVEVRGDKSIEEIEGVTRASESDWGQEFLD
MIIAARVVEDLDDALDHIRTYGSNHTDAIIAEDDAAVARFFQRLDSAILIHNASTQFADGGEFGMGAEIGIATGKMHARG
PVGSEQLTSFKYLVTGDGTIRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=406, Percent_Identity=36.9458128078818, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI21361368, Length=406, Percent_Identity=36.9458128078818, Blast_Score=258, Evalue=8e-69,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=43.75, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17569967, Length=411, Percent_Identity=34.5498783454988, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17569969, Length=411, Percent_Identity=34.5498783454988, Blast_Score=252, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=37.995337995338, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI21357643, Length=416, Percent_Identity=37.7403846153846, Blast_Score=281, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_JANSC (Q28Q10)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510227.1
- HSSP:   P54886
- ProteinModelPortal:   Q28Q10
- SMR:   Q28Q10
- STRING:   Q28Q10
- GeneID:   3934741
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2285
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- PhylomeDB:   Q28Q10
- ProtClustDB:   PRK00197
- BioCyc:   JSP290400:JANN_2285-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44909; Mature: 44909

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLTDNVPALMAEIGARAKNASAELAFASPEAKFTALTAAADAVWARRADIIAANEKDM
CCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCHH
AFGREKGLSDAMLDRLMLDEARVEGIVTGLRAVAGQEDPVGHVMTEWEMESGLHIKRVRT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEHHC
PLGVIGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSSGALADCLQEGLRTAGLPE
CCCEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCH
DAVQRVPTRDRAAVSEMLAMTETIDVIVPRGGKGLVALVQREARVPVFAHLEGICHIYLD
HHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHEEHHHCCCCHHEECCCEEEEEEE
ASADPEKALRVVLNAKTRRTGICGSAECLLIHQDIADTIGQGVVRALIDAGVEVRGDKSI
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEECCCCCH
EEIEGVTRASESDWGQEFLDMIIAARVVEDLDDALDHIRTYGSNHTDAIIAEDDAAVARF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
FQRLDSAILIHNASTQFADGGEFGMGAEIGIATGKMHARGPVGSEQLTSFKYLVTGDGTI
HHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEECCEECCCCCCHHHHCEEEEEEECCCCC
RG
CC
>Mature Secondary Structure
MKDLTDNVPALMAEIGARAKNASAELAFASPEAKFTALTAAADAVWARRADIIAANEKDM
CCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCHH
AFGREKGLSDAMLDRLMLDEARVEGIVTGLRAVAGQEDPVGHVMTEWEMESGLHIKRVRT
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEHHC
PLGVIGVIYESRPNVTADAGALCLKSGNAVILRGGSESFHSSGALADCLQEGLRTAGLPE
CCCEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCH
DAVQRVPTRDRAAVSEMLAMTETIDVIVPRGGKGLVALVQREARVPVFAHLEGICHIYLD
HHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHEEHHHCCCCHHEECCCEEEEEEE
ASADPEKALRVVLNAKTRRTGICGSAECLLIHQDIADTIGQGVVRALIDAGVEVRGDKSI
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEECCCCCH
EEIEGVTRASESDWGQEFLDMIIAARVVEDLDDALDHIRTYGSNHTDAIIAEDDAAVARF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
FQRLDSAILIHNASTQFADGGEFGMGAEIGIATGKMHARGPVGSEQLTSFKYLVTGDGTI
HHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEECCEECCCCCCHHHHCEEEEEEECCCCC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA