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The map label for this gene is proB

Identifier: 89054775

GI number: 89054775

Start: 2289765

End: 2290871

Strand: Direct

Name: proB

Synonym: Jann_2284

Alternate gene names: 89054775

Gene position: 2289765-2290871 (Clockwise)

Preceding gene: 161898395

Following gene: 89054776

Centisome position: 53.03

GC content: 67.12

Gene sequence:

>1107_bases
ATGGCGTCCCTGACGGGGGCCAAGCGCGTTGTCGTCAAGATCGGCTCGGCCCTGTTGGTGGACCGACACACGGGCGATTT
GCGTGCCGATTGGCTGGCGGCTTTGGCCGATGACATCGCAGCGTTGAAGGGGGCGGGCACGGACGTCATTCTGGTCTCCT
CCGGTGCGATTGCATTGGGTCGTGGCGTGTTGGCGTTGGGCGATGGGGCCCTGTCGCTGGATGAGGCGCAGGCCGCGGCG
GCGGTGGGGCAGATCCGGCTGGCGCGCGCGTATGAGGAGGTTCTGGCCCCCCTCGGCCTGACGTCGGCGCAGATCCTGCT
GACGCTGGACGACAGCGCGGATCGCAGGCGTTACCTCAACACCCGCGCCACCTTCGCGGCCCTTCTGGCGCGCGGGGCCG
TGCCGATTGTGAACGAGAACGACACCATCGCGACGGATGAGATCCGCTATGGCGACAACGACCGTTTGGCCGCCAACGTG
GCCGTGATGGCGGGGGCGGATATCTGTGTTTTGTTGTCGGATGTGGACGGGCTCTACACCGCCAACCCCAACGACGACAC
CACGGCGGCGCATTTGCCGGTGATCGACACCATCACACCGGAGATCGAGGCGATGGCGGGGGATGCGGGCTCGGGCCTGT
CAAAGGGTGGGATGAAAACCAAGGTTCTGGCCGCACGCACCGCGACGGCGGCGGGCTGCGCCATGGCGATCACCCAGGGG
GCGATTGCGAACCCGCTGCGCGCATTGGATGACGGGGCAAGGGCCACGTGGTTCACGGCCACCGTGTCACCGCAACAGGC
CCGCAAGGCGTGGATCGGGGCGATGAAACCCAAAGGGTCTCTGCGTTTGGATGCGGGCGCGGCTGCGGCGCTGAGCCGCG
GGAAGTCGCTGCTGCCTGCGGGCGTCGTTGCCGTCACAGGCGATTTCCTGCGCGGCGATCCGGTCCGGCTGGAAGCCCCC
GATGGAGCCGCCATTGGCATCGGCCTGAGCCGCTACGGCGCGAAAGAGGCCCTTGCCATCCAAGGCCATCAATCCGATGA
AATCCCGGAGATCCTGGGCTATCCAGGTCGCGCCGCCCTGGTGCACCGTGACGATATGGTGCTCTAG

Upstream 100 bases:

>100_bases
GCGTTGCCCGCACCGGCGTGACGGAGGTGTTGCGCGTCTTGCGGGCGCGGGTCGATGCGGACCGGAAAGCGGCCCGGGAT
GCGCTGGAGGAGCCGGACCG

Downstream 100 bases:

>100_bases
CCCCTTCACAGACGCTCAAAACCCAATATCCTGTCCCCTCCGCCCCTGCATCGAGGTGTCCCATGAAAGACCTGACCGAT
AACGTCCCCGCCCTGATGGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MASLTGAKRVVVKIGSALLVDRHTGDLRADWLAALADDIAALKGAGTDVILVSSGAIALGRGVLALGDGALSLDEAQAAA
AVGQIRLARAYEEVLAPLGLTSAQILLTLDDSADRRRYLNTRATFAALLARGAVPIVNENDTIATDEIRYGDNDRLAANV
AVMAGADICVLLSDVDGLYTANPNDDTTAAHLPVIDTITPEIEAMAGDAGSGLSKGGMKTKVLAARTATAAGCAMAITQG
AIANPLRALDDGARATWFTATVSPQQARKAWIGAMKPKGSLRLDAGAAAALSRGKSLLPAGVVAVTGDFLRGDPVRLEAP
DGAAIGIGLSRYGAKEALAIQGHQSDEIPEILGYPGRAALVHRDDMVL

Sequences:

>Translated_368_residues
MASLTGAKRVVVKIGSALLVDRHTGDLRADWLAALADDIAALKGAGTDVILVSSGAIALGRGVLALGDGALSLDEAQAAA
AVGQIRLARAYEEVLAPLGLTSAQILLTLDDSADRRRYLNTRATFAALLARGAVPIVNENDTIATDEIRYGDNDRLAANV
AVMAGADICVLLSDVDGLYTANPNDDTTAAHLPVIDTITPEIEAMAGDAGSGLSKGGMKTKVLAARTATAAGCAMAITQG
AIANPLRALDDGARATWFTATVSPQQARKAWIGAMKPKGSLRLDAGAAAALSRGKSLLPAGVVAVTGDFLRGDPVRLEAP
DGAAIGIGLSRYGAKEALAIQGHQSDEIPEILGYPGRAALVHRDDMVL
>Mature_367_residues
ASLTGAKRVVVKIGSALLVDRHTGDLRADWLAALADDIAALKGAGTDVILVSSGAIALGRGVLALGDGALSLDEAQAAAA
VGQIRLARAYEEVLAPLGLTSAQILLTLDDSADRRRYLNTRATFAALLARGAVPIVNENDTIATDEIRYGDNDRLAANVA
VMAGADICVLLSDVDGLYTANPNDDTTAAHLPVIDTITPEIEAMAGDAGSGLSKGGMKTKVLAARTATAAGCAMAITQGA
IANPLRALDDGARATWFTATVSPQQARKAWIGAMKPKGSLRLDAGAAAALSRGKSLLPAGVVAVTGDFLRGDPVRLEAPD
GAAIGIGLSRYGAKEALAIQGHQSDEIPEILGYPGRAALVHRDDMVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=298, Percent_Identity=30.2013422818792, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI62912457, Length=296, Percent_Identity=30.4054054054054, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786437, Length=368, Percent_Identity=40.7608695652174, Blast_Score=240, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI17569967, Length=254, Percent_Identity=34.6456692913386, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=254, Percent_Identity=34.6456692913386, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=426, Percent_Identity=32.3943661971831, Blast_Score=185, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=31.8289786223278, Blast_Score=173, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21357643, Length=292, Percent_Identity=30.4794520547945, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_JANSC (Q28Q11)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510226.1
- ProteinModelPortal:   Q28Q11
- SMR:   Q28Q11
- STRING:   Q28Q11
- GeneID:   3934740
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2284
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q28Q11
- ProtClustDB:   PRK05429
- BioCyc:   JSP290400:JANN_2284-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 37655; Mature: 37524

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLTGAKRVVVKIGSALLVDRHTGDLRADWLAALADDIAALKGAGTDVILVSSGAIALG
CCCCCCHHEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHC
RGVLALGDGALSLDEAQAAAAVGQIRLARAYEEVLAPLGLTSAQILLTLDDSADRRRYLN
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHH
TRATFAALLARGAVPIVNENDTIATDEIRYGDNDRLAANVAVMAGADICVLLSDVDGLYT
HHHHHHHHHHCCCCEEECCCCCEEECCEEECCCCCEEEEEEEEECCCEEEEEECCCCEEE
ANPNDDTTAAHLPVIDTITPEIEAMAGDAGSGLSKGGMKTKVLAARTATAAGCAMAITQG
CCCCCCCCEEECCEEECCCCCHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCC
AIANPLRALDDGARATWFTATVSPQQARKAWIGAMKPKGSLRLDAGAAAALSRGKSLLPA
HHHCHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCC
GVVAVTGDFLRGDPVRLEAPDGAAIGIGLSRYGAKEALAIQGHQSDEIPEILGYPGRAAL
CEEEEECHHHCCCCEEEECCCCCEEEEEHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEE
VHRDDMVL
EECCCCCC
>Mature Secondary Structure 
ASLTGAKRVVVKIGSALLVDRHTGDLRADWLAALADDIAALKGAGTDVILVSSGAIALG
CCCCCHHEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHC
RGVLALGDGALSLDEAQAAAAVGQIRLARAYEEVLAPLGLTSAQILLTLDDSADRRRYLN
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHH
TRATFAALLARGAVPIVNENDTIATDEIRYGDNDRLAANVAVMAGADICVLLSDVDGLYT
HHHHHHHHHHCCCCEEECCCCCEEECCEEECCCCCEEEEEEEEECCCEEEEEECCCCEEE
ANPNDDTTAAHLPVIDTITPEIEAMAGDAGSGLSKGGMKTKVLAARTATAAGCAMAITQG
CCCCCCCCEEECCEEECCCCCHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCC
AIANPLRALDDGARATWFTATVSPQQARKAWIGAMKPKGSLRLDAGAAAALSRGKSLLPA
HHHCHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCCC
GVVAVTGDFLRGDPVRLEAPDGAAIGIGLSRYGAKEALAIQGHQSDEIPEILGYPGRAAL
CEEEEECHHHCCCCEEEECCCCCEEEEEHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEE
VHRDDMVL
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA