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The map label for this gene is ntrB [H]

Identifier: 89054728

GI number: 89054728

Start: 2241755

End: 2242822

Strand: Direct

Name: ntrB [H]

Synonym: Jann_2237

Alternate gene names: 89054728

Gene position: 2241755-2242822 (Clockwise)

Preceding gene: 89054727

Following gene: 89054729

Centisome position: 51.92

GC content: 62.92

Gene sequence:

>1068_bases
ATGAGCACGCTGACGAACGCGCTTTGGGTCTCCCTCCCAATCCCTGCGCTGATCCTCGACGCCGAGGATCGCATCCGCGA
CCTTAATCCCGTGGCCGAAACGTTCCTGAATGCGTCACAAAAGTCCCTGGATGGGGCGGTGATGTGGGACAGGATCCACA
TGTCGACGCCGCTCAAGGACAGCGTGGCGCGGGTGCGCAATTCAGCGTCCTCCCTGCATGTGAACGCGGTGGATGTGGGC
TCGGGCACGCGCAAACCGGTGTCATGTGACGTGCAGATCTCGGCCCTTGCCGACCGGCCCGACCACGTTCTTGTGCTGAT
GGAAAACCGGGAGCTTGTGGGCCGCATGGACCGTGCGATGTCGTCAAAAACGGCGGCGAAATCGGCGATCGGCATGGCCG
AGATGCTGGCCCATGAGATCAAGAACCCCCTGGCCGGCATCACCGGGGCGGCGCAGCTTATTTCCATGAACGCAAATGCC
GAGGATCGGGAGTTGACCGACCTGATCGTGGCAGAGACCCGTCGCGTGCTGAAATTGTTGGAGCAGGTCGAGGATTTCGG
CAATGTGCGCCCGCCCGAGCGACGCGCCGTCAACATCCATGACGTGCTGGACCACGCGCGGCGCTCTGCCTCCGTCGGGT
TTGCCGCGCATATGAATATCATTGAGGATTACGACCCGTCGCTGCCCCACACATGGGCGGACCCCGATCAATTGCAGCAA
GTCATCATGAACCTGCTGAAGAACGCCGCGGAGGCCGGGCAATCCGGCGGCACGATCTGCCTGCGGACGTTTTACGAGAT
GTCGTTGAAGGTACGCCGCCCCGATGGCTCCGCCGCCGCCGTCCCGCTTCAGATCGAAGTGAGCGATGACGGCTCGGGTA
TCGCGCCCGAACTGGTCAAGGACGTGTTTGAACCGTTCGTGTCCGGCCGCGAGAATGGCACGGGCCTGGGCCTTGCGCTG
ATCTCGAAGATCGTGGCCGACCATGATGGGTGGGTGGCCGTCGACAGCGTGCCGGGCCGCACCACGTTCCGCATTTCCCT
GCCAATTGCACCCAAGGAGGCCTCGTGA

Upstream 100 bases:

>100_bases
CGCCCGCATTGCGCAAAGAGGTATTGACCACCAAAGATCCCGGTGCCGTGTTGAAGGCCCTGCCGCTGGCCCTCGCACAA
ACGTCCCAAAGGGCCGCCGC

Downstream 100 bases:

>100_bases
TGCCCACGCAAAGCCCCCGTGCGCCACGACACACCCTATTGAAAGGCCGCTGATCCATGGACGGAACCGTACTTGTCGCC
GATGATGACCGCACGATCCG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSTLTNALWVSLPIPALILDAEDRIRDLNPVAETFLNASQKSLDGAVMWDRIHMSTPLKDSVARVRNSASSLHVNAVDVG
SGTRKPVSCDVQISALADRPDHVLVLMENRELVGRMDRAMSSKTAAKSAIGMAEMLAHEIKNPLAGITGAAQLISMNANA
EDRELTDLIVAETRRVLKLLEQVEDFGNVRPPERRAVNIHDVLDHARRSASVGFAAHMNIIEDYDPSLPHTWADPDQLQQ
VIMNLLKNAAEAGQSGGTICLRTFYEMSLKVRRPDGSAAAVPLQIEVSDDGSGIAPELVKDVFEPFVSGRENGTGLGLAL
ISKIVADHDGWVAVDSVPGRTTFRISLPIAPKEAS

Sequences:

>Translated_355_residues
MSTLTNALWVSLPIPALILDAEDRIRDLNPVAETFLNASQKSLDGAVMWDRIHMSTPLKDSVARVRNSASSLHVNAVDVG
SGTRKPVSCDVQISALADRPDHVLVLMENRELVGRMDRAMSSKTAAKSAIGMAEMLAHEIKNPLAGITGAAQLISMNANA
EDRELTDLIVAETRRVLKLLEQVEDFGNVRPPERRAVNIHDVLDHARRSASVGFAAHMNIIEDYDPSLPHTWADPDQLQQ
VIMNLLKNAAEAGQSGGTICLRTFYEMSLKVRRPDGSAAAVPLQIEVSDDGSGIAPELVKDVFEPFVSGRENGTGLGLAL
ISKIVADHDGWVAVDSVPGRTTFRISLPIAPKEAS
>Mature_354_residues
STLTNALWVSLPIPALILDAEDRIRDLNPVAETFLNASQKSLDGAVMWDRIHMSTPLKDSVARVRNSASSLHVNAVDVGS
GTRKPVSCDVQISALADRPDHVLVLMENRELVGRMDRAMSSKTAAKSAIGMAEMLAHEIKNPLAGITGAAQLISMNANAE
DRELTDLIVAETRRVLKLLEQVEDFGNVRPPERRAVNIHDVLDHARRSASVGFAAHMNIIEDYDPSLPHTWADPDQLQQV
IMNLLKNAAEAGQSGGTICLRTFYEMSLKVRRPDGSAAAVPLQIEVSDDGSGIAPELVKDVFEPFVSGRENGTGLGLALI
SKIVADHDGWVAVDSVPGRTTFRISLPIAPKEAS

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=346, Percent_Identity=32.9479768786127, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788549, Length=347, Percent_Identity=25.9365994236311, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=29.9107142857143, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=28.8617886178862, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=378, Percent_Identity=21.6931216931217, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=254, Percent_Identity=25.9842519685039, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=27.5109170305677, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=25.6521739130435, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=23.8683127572016, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38385; Mature: 38254

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTNALWVSLPIPALILDAEDRIRDLNPVAETFLNASQKSLDGAVMWDRIHMSTPLKD
CCCCCCHHEEECCCCEEEECCHHHHHCCCHHHHHHHCCHHHCCCCCEEEEEHCCCCCHHH
SVARVRNSASSLHVNAVDVGSGTRKPVSCDVQISALADRPDHVLVLMENRELVGRMDRAM
HHHHHHCCCCCEEEEEEECCCCCCCCEEECEEEEEECCCCCEEEEEECCCHHHHHHHHHH
SSKTAAKSAIGMAEMLAHEIKNPLAGITGAAQLISMNANAEDRELTDLIVAETRRVLKLL
HHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
EQVEDFGNVRPPERRAVNIHDVLDHARRSASVGFAAHMNIIEDYDPSLPHTWADPDQLQQ
HHHHHHCCCCCCHHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
VIMNLLKNAAEAGQSGGTICLRTFYEMSLKVRRPDGSAAAVPLQIEVSDDGSGIAPELVK
HHHHHHHHHHHCCCCCCHHHHHHHHHCEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHH
DVFEPFVSGRENGTGLGLALISKIVADHDGWVAVDSVPGRTTFRISLPIAPKEAS
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
STLTNALWVSLPIPALILDAEDRIRDLNPVAETFLNASQKSLDGAVMWDRIHMSTPLKD
CCCCCHHEEECCCCEEEECCHHHHHCCCHHHHHHHCCHHHCCCCCEEEEEHCCCCCHHH
SVARVRNSASSLHVNAVDVGSGTRKPVSCDVQISALADRPDHVLVLMENRELVGRMDRAM
HHHHHHCCCCCEEEEEEECCCCCCCCEEECEEEEEECCCCCEEEEEECCCHHHHHHHHHH
SSKTAAKSAIGMAEMLAHEIKNPLAGITGAAQLISMNANAEDRELTDLIVAETRRVLKLL
HHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
EQVEDFGNVRPPERRAVNIHDVLDHARRSASVGFAAHMNIIEDYDPSLPHTWADPDQLQQ
HHHHHHCCCCCCHHHEEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
VIMNLLKNAAEAGQSGGTICLRTFYEMSLKVRRPDGSAAAVPLQIEVSDDGSGIAPELVK
HHHHHHHHHHHCCCCCCHHHHHHHHHCEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHH
DVFEPFVSGRENGTGLGLALISKIVADHDGWVAVDSVPGRTTFRISLPIAPKEAS
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]