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The map label for this gene is ntrC [H]

Identifier: 89054729

GI number: 89054729

Start: 2242879

End: 2244258

Strand: Direct

Name: ntrC [H]

Synonym: Jann_2238

Alternate gene names: 89054729

Gene position: 2242879-2244258 (Clockwise)

Preceding gene: 89054728

Following gene: 89054730

Centisome position: 51.94

GC content: 62.61

Gene sequence:

>1380_bases
ATGGACGGAACCGTACTTGTCGCCGATGATGACCGCACGATCCGCACCGTGTTGACCCAGGCCCTGACGCGCGCAGGCTG
CAAGGTCCACGCGACTTCCAGCCTTGTGACGCTGATGCGCTGGGTGGAGGAGGGAAAGGGCGATCTGGTGATCTCCGACG
TGGTCATGCCCGATGGCAATGGTCTCGACACCTTGCCCCAGATTACCGAGGCGCGACCGGGCCTGCCGGTGATTATCATT
TCGGCCCAGAACACGATCATGACGGCGATCCAGGCGACCGAAAAGGACGCCTACGATTATCTGCCAAAACCGTTTGATCT
GCCGGATCTGATGAAACGGGCGGCGCGCGCGCTGGATCTCAAGCGCCGTGCGCCCGTCGCCGCGAAGGCATCGGAACCGG
CACTGCCCGCGACCGGTGAGGATCTGCCATTGGTGGGGCGCACGCCCGCGATGCAGGCCCTTTATCGCCTTGTGGCGCGG
GTGATGAATACCGATCTGACGGTGCTGGTCACAGGCGAAAGCGGCACCGGCAAGTCCTTGATCGCGCGTGCAATTCACGA
CTTCTCGGACCGTCGGACATTGCCTTTCGTCGTGGCTTCCGCCGCTGATCTGCGCGGCGCGGATGGGCCCTCGATGATCC
TCAGTCGCGCCAAGGGCGGCTCGATCCTGTTTGATGAGGTCGGCGATCTTGATGAGGAGGCGCAGGCCAAAATCGTCCGC
ATGCTTGATGCGATGCCGCAGGCCAATCCGCGCATCATGGCGACCTCCCAGAAAGACCTGACCGAGAAGATGGAGGAGGG
GCGCTTTCGCCAGGATCTCTACTATCGCTTGGGCGGTGTCGGGATCGCGGTGCCGGCGTTGCGCGAACGGGTCGATGACA
TCCCGCTTCTGGCAAGCCACTTTCTGGCGCGGGCGGAGCGGGACGGGGCCGCCCCCCGCCGGTTCTCGGACGCTGCGATG
GAACTGGTGCGCGCCTATTCCTGGCCCGGCAATGTGCGCCAGCTGGAAAACTCCATCCGCCGCCTGACGGTCACCGCGCA
GGTCGAAGAGATCAGCCGCGCGGAAGTGGAGGTCGTTTTGGGCTCGCAACCGGCGATTGAGCCGCTGATGTCGGGCGCGA
GTGAGGGCGACAAACTCAGCGCATCGGTGGCCAAACATCTGCGCCGCTACTTCGATCTTCACGGCGGCGTCCTGCCTGCA
CCCGGCCTATATGCCCGTATTCTAAAGGAAGTTGAGGCCCCATTGATCGAAATCGCACTGGATGCGACGGCGGGCAATCA
AGCTAAATGTGCCGATCTTCTGGGGATCAACCGCAATACCTTGCGTAAAAAGATCACCGATCTGGATATTCGCGTGACAC
GCCGCCGCAAACTGGTGTAA

Upstream 100 bases:

>100_bases
CGTTCCGCATTTCCCTGCCAATTGCACCCAAGGAGGCCTCGTGATGCCCACGCAAAGCCCCCGTGCGCCACGACACACCC
TATTGAAAGGCCGCTGATCC

Downstream 100 bases:

>100_bases
TAAAGCCACACGCGTGGCACATCGGGAACAGGCATCCCGATGCGCACTGGCCCTGTCAGGCCAAGCAAGCGCGCGCCAGA
AGGATGCGGTGGGGGATGCG

Product: two component sigma54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLVTLMRWVEEGKGDLVISDVVMPDGNGLDTLPQITEARPGLPVIII
SAQNTIMTAIQATEKDAYDYLPKPFDLPDLMKRAARALDLKRRAPVAAKASEPALPATGEDLPLVGRTPAMQALYRLVAR
VMNTDLTVLVTGESGTGKSLIARAIHDFSDRRTLPFVVASAADLRGADGPSMILSRAKGGSILFDEVGDLDEEAQAKIVR
MLDAMPQANPRIMATSQKDLTEKMEEGRFRQDLYYRLGGVGIAVPALRERVDDIPLLASHFLARAERDGAAPRRFSDAAM
ELVRAYSWPGNVRQLENSIRRLTVTAQVEEISRAEVEVVLGSQPAIEPLMSGASEGDKLSASVAKHLRRYFDLHGGVLPA
PGLYARILKEVEAPLIEIALDATAGNQAKCADLLGINRNTLRKKITDLDIRVTRRRKLV

Sequences:

>Translated_459_residues
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLVTLMRWVEEGKGDLVISDVVMPDGNGLDTLPQITEARPGLPVIII
SAQNTIMTAIQATEKDAYDYLPKPFDLPDLMKRAARALDLKRRAPVAAKASEPALPATGEDLPLVGRTPAMQALYRLVAR
VMNTDLTVLVTGESGTGKSLIARAIHDFSDRRTLPFVVASAADLRGADGPSMILSRAKGGSILFDEVGDLDEEAQAKIVR
MLDAMPQANPRIMATSQKDLTEKMEEGRFRQDLYYRLGGVGIAVPALRERVDDIPLLASHFLARAERDGAAPRRFSDAAM
ELVRAYSWPGNVRQLENSIRRLTVTAQVEEISRAEVEVVLGSQPAIEPLMSGASEGDKLSASVAKHLRRYFDLHGGVLPA
PGLYARILKEVEAPLIEIALDATAGNQAKCADLLGINRNTLRKKITDLDIRVTRRRKLV
>Mature_459_residues
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLVTLMRWVEEGKGDLVISDVVMPDGNGLDTLPQITEARPGLPVIII
SAQNTIMTAIQATEKDAYDYLPKPFDLPDLMKRAARALDLKRRAPVAAKASEPALPATGEDLPLVGRTPAMQALYRLVAR
VMNTDLTVLVTGESGTGKSLIARAIHDFSDRRTLPFVVASAADLRGADGPSMILSRAKGGSILFDEVGDLDEEAQAKIVR
MLDAMPQANPRIMATSQKDLTEKMEEGRFRQDLYYRLGGVGIAVPALRERVDDIPLLASHFLARAERDGAAPRRFSDAAM
ELVRAYSWPGNVRQLENSIRRLTVTAQVEEISRAEVEVVLGSQPAIEPLMSGASEGDKLSASVAKHLRRYFDLHGGVLPA
PGLYARILKEVEAPLIEIALDATAGNQAKCADLLGINRNTLRKKITDLDIRVTRRRKLV

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=471, Percent_Identity=35.031847133758, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790299, Length=482, Percent_Identity=33.195020746888, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1788550, Length=481, Percent_Identity=30.7692307692308, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1788905, Length=474, Percent_Identity=32.7004219409283, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789233, Length=337, Percent_Identity=32.0474777448071, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082152, Length=249, Percent_Identity=35.3413654618474, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082117, Length=228, Percent_Identity=36.4035087719298, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=37.3913043478261, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787583, Length=220, Percent_Identity=32.7272727272727, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=31.4516129032258, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=26.969696969697, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081858, Length=323, Percent_Identity=25.6965944272446, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789828, Length=273, Percent_Identity=25.6410256410256, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786784, Length=127, Percent_Identity=32.2834645669291, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49952; Mature: 49952

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLVTLMRWVEEGKGDLVISDVVMPDGN
CCCCEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GLDTLPQITEARPGLPVIIISAQNTIMTAIQATEKDAYDYLPKPFDLPDLMKRAARALDL
CCCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KRRAPVAAKASEPALPATGEDLPLVGRTPAMQALYRLVARVMNTDLTVLVTGESGTGKSL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
IARAIHDFSDRRTLPFVVASAADLRGADGPSMILSRAKGGSILFDEVGDLDEEAQAKIVR
HHHHHHHHCCCCCCCEEEECHHHCCCCCCHHHHHHHCCCCCEEHHHHCCCCHHHHHHHHH
MLDAMPQANPRIMATSQKDLTEKMEEGRFRQDLYYRLGGVGIAVPALRERVDDIPLLASH
HHHHCCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHH
FLARAERDGAAPRRFSDAAMELVRAYSWPGNVRQLENSIRRLTVTAQVEEISRAEVEVVL
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEHHHHHHHHCEEEEEE
GSQPAIEPLMSGASEGDKLSASVAKHLRRYFDLHGGVLPAPGLYARILKEVEAPLIEIAL
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEE
DATAGNQAKCADLLGINRNTLRKKITDLDIRVTRRRKLV
ECCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEHHHHCCC
>Mature Secondary Structure
MDGTVLVADDDRTIRTVLTQALTRAGCKVHATSSLVTLMRWVEEGKGDLVISDVVMPDGN
CCCCEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCC
GLDTLPQITEARPGLPVIIISAQNTIMTAIQATEKDAYDYLPKPFDLPDLMKRAARALDL
CCCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KRRAPVAAKASEPALPATGEDLPLVGRTPAMQALYRLVARVMNTDLTVLVTGESGTGKSL
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
IARAIHDFSDRRTLPFVVASAADLRGADGPSMILSRAKGGSILFDEVGDLDEEAQAKIVR
HHHHHHHHCCCCCCCEEEECHHHCCCCCCHHHHHHHCCCCCEEHHHHCCCCHHHHHHHHH
MLDAMPQANPRIMATSQKDLTEKMEEGRFRQDLYYRLGGVGIAVPALRERVDDIPLLASH
HHHHCCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHH
FLARAERDGAAPRRFSDAAMELVRAYSWPGNVRQLENSIRRLTVTAQVEEISRAEVEVVL
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEHHHHHHHHCEEEEEE
GSQPAIEPLMSGASEGDKLSASVAKHLRRYFDLHGGVLPAPGLYARILKEVEAPLIEIAL
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEE
DATAGNQAKCADLLGINRNTLRKKITDLDIRVTRRRKLV
ECCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]