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The map label for this gene is prpB [C]

Identifier: 89054091

GI number: 89054091

Start: 1582838

End: 1583668

Strand: Direct

Name: prpB [C]

Synonym: Jann_1600

Alternate gene names: 89054091

Gene position: 1582838-1583668 (Clockwise)

Preceding gene: 89054090

Following gene: 89054092

Centisome position: 36.66

GC content: 69.19

Gene sequence:

>831_bases
ATGACCTTGTCCCAACGACACGAGGCCTTCGCGCGCCTTCACCAGAGTGGGTGTTTCGTGATGCCCAACCCCTGGGATCG
CGGCTCGGCCCGGATGATGGCGGCGTCGGGTGCTTTGGCGCTGGCCACGACCTCGTCGGGTCACGCGTTCACGCTGGGAC
GCGCGGATATGGGCGGGGTGGACCGCGATGAGGCACTGGCCCACGCCCAGGACATCCTCAGCGCCACCGCGCTGCCGGTC
TCGGGCGATTTCGAGAACGGCTTCGGCGATGATCCAGACACGGTGGCCGAGACGATCCGCATGGCGGCGGAGGTCGGCCT
CTCGGGCTGCGGGATCGAGGATATGGCGTTTGAAGGCGGGCTGCGCGCTTATGACTTTGACCTGAGCGTCGAGCGAATCA
AAGCGGCGGTGGCGGCGGTTCAAAGCCTTGGGCGGCCCTTCGTGCTGACGGCCCGCGCCGATGGGGTGATGAACGGGCTC
TACGATCTGGACGAGGGGATTGCCCGGCTGAAGGCGTTTGAGGCGGCGGGCGCGGAGTGTCTGTACCTGCCGGTCCCGCC
GGGGCGCGCCGAATTGGCGCGGGTGCTGGCGGCGGTGAGCGCCCCGGTCAACGTGTTGGCGGCGGGCGCGTTGCGCGATC
TGCCGGTGGGCGATCTGGCCGCCATGGGCGTGCGCCGCGTGTCCCTGGGCGGGCAGGTGGCGCGTGTGACGCACGCCGCC
ATCCGCGACGCGATGGCGGGCGTGATGGCCGGGGATCTGACGGCGCTGAAAGGCAGTGCTTCCGGGGATGAGATTGATGC
CATGCTGGGGAAGGGTGCCGGAGATGACTGA

Upstream 100 bases:

>100_bases
CCACGCGGATCTGGTTCAACCATTGGACCGGGCGGCTTTATCTGGCGCTTGTCTTGATCCCACATAAAATCATTGTAAAA
CTCGCACTTAGGAGGTTGGC

Downstream 100 bases:

>100_bases
CATCATGGAGAAAAAAGGTCGCCGGGGCGGGGGTCGTGCGGCACGCGTTGCGGCGCGGGCCGCGGCCTTGCCGGACGCGA
TGCGCCCAATCCGGCCCGGC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTLSQRHEAFARLHQSGCFVMPNPWDRGSARMMAASGALALATTSSGHAFTLGRADMGGVDRDEALAHAQDILSATALPV
SGDFENGFGDDPDTVAETIRMAAEVGLSGCGIEDMAFEGGLRAYDFDLSVERIKAAVAAVQSLGRPFVLTARADGVMNGL
YDLDEGIARLKAFEAAGAECLYLPVPPGRAELARVLAAVSAPVNVLAAGALRDLPVGDLAAMGVRRVSLGGQVARVTHAA
IRDAMAGVMAGDLTALKGSASGDEIDAMLGKGAGDD

Sequences:

>Translated_276_residues
MTLSQRHEAFARLHQSGCFVMPNPWDRGSARMMAASGALALATTSSGHAFTLGRADMGGVDRDEALAHAQDILSATALPV
SGDFENGFGDDPDTVAETIRMAAEVGLSGCGIEDMAFEGGLRAYDFDLSVERIKAAVAAVQSLGRPFVLTARADGVMNGL
YDLDEGIARLKAFEAAGAECLYLPVPPGRAELARVLAAVSAPVNVLAAGALRDLPVGDLAAMGVRRVSLGGQVARVTHAA
IRDAMAGVMAGDLTALKGSASGDEIDAMLGKGAGDD
>Mature_275_residues
TLSQRHEAFARLHQSGCFVMPNPWDRGSARMMAASGALALATTSSGHAFTLGRADMGGVDRDEALAHAQDILSATALPVS
GDFENGFGDDPDTVAETIRMAAEVGLSGCGIEDMAFEGGLRAYDFDLSVERIKAAVAAVQSLGRPFVLTARADGVMNGLY
DLDEGIARLKAFEAAGAECLYLPVPPGRAELARVLAAVSAPVNVLAAGALRDLPVGDLAAMGVRRVSLGGQVARVTHAAI
RDAMAGVMAGDLTALKGSASGDEIDAMLGKGAGDD

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28329; Mature: 28198

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSQRHEAFARLHQSGCFVMPNPWDRGSARMMAASGALALATTSSGHAFTLGRADMGGV
CCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCC
DRDEALAHAQDILSATALPVSGDFENGFGDDPDTVAETIRMAAEVGLSGCGIEDMAFEGG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
LRAYDFDLSVERIKAAVAAVQSLGRPFVLTARADGVMNGLYDLDEGIARLKAFEAAGAEC
CEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCE
LYLPVPPGRAELARVLAAVSAPVNVLAAGALRDLPVGDLAAMGVRRVSLGGQVARVTHAA
EEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IRDAMAGVMAGDLTALKGSASGDEIDAMLGKGAGDD
HHHHHHHHHHCCHHHCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TLSQRHEAFARLHQSGCFVMPNPWDRGSARMMAASGALALATTSSGHAFTLGRADMGGV
CCCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCC
DRDEALAHAQDILSATALPVSGDFENGFGDDPDTVAETIRMAAEVGLSGCGIEDMAFEGG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
LRAYDFDLSVERIKAAVAAVQSLGRPFVLTARADGVMNGLYDLDEGIARLKAFEAAGAEC
CEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCE
LYLPVPPGRAELARVLAAVSAPVNVLAAGALRDLPVGDLAAMGVRRVSLGGQVARVTHAA
EEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IRDAMAGVMAGDLTALKGSASGDEIDAMLGKGAGDD
HHHHHHHHHHCCHHHCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]