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The map label for this gene is opuAB [H]

Identifier: 89053990

GI number: 89053990

Start: 1469888

End: 1470871

Strand: Direct

Name: opuAB [H]

Synonym: Jann_1499

Alternate gene names: 89053990

Gene position: 1469888-1470871 (Clockwise)

Preceding gene: 89053989

Following gene: 89053991

Centisome position: 34.04

GC content: 60.47

Gene sequence:

>984_bases
ATGTGGTGGCAGAATTTTCGGCAGTGGTGGCGCGACGACGGCATCTCGGAATGCTCGTTCCCCAATGATCCGGATGTCAC
CGGCCCGATCACCTTCCTGACCGAGTGCAAATTGCGCATCGGCGACGGGGCGGAGGCGGTGTTTGACTGGCTGCAAAGCA
ACGGCGCGTTCATTTTTGACGCGATGGCCGTGGCGCTGGAAGCGATGATCGAGGTGTTTCTGGCCATCCTGCAAGAACCG
CCCAACACCTCTTGGTTTCAGGATATCCGCCTGCGCGACGCGGCATTTCTCTACCCGCAGGACTGGCATCCCTTTCTGGT
CATTGCGATTTTCACGGCGCTCGCCTGGTGGATGCATCGCGGTTGGAAAGTGCCCATGCTGGTGGCCGTCGGGTTCCTGT
TCATCGTTAATCAGGACTATTGGGAGGAAACGACTGAAAGCCTGACCCTCGTCCTGTCGGCCTGCGTCGTCTGCATGGGC
GTGGGCGTGCCCATCGGCATCGCCGTCGCTCACAGGCCCCGCCTCTATGCGTGGATGCGGCCCGTTCTGGACCTCATGCA
AACCCTGCCGACCTTCGTCTATCTCATTCCGGCCATTGTGTTTTTCGGCATCGGCATGGTGCCGGGCCTGATCGCTACGG
TGATCTTCGTTCTGCCCGCGCCGATCCGGCTGACCCATCTCGGCATCTCCTCTACACCGCAAAGCCTGCTTGAGGCGGCG
GATGCATTCGGCGCGACCGGCTCCCAAAAGCTCTGGAAAGTAGAGCTGCCCTACGCGCTGCCGCAGATCATGACGGGCCT
GAACCAGACGATCATGTTGTCGCTCTCGATGGTGGTGATCGCAGCGCTTGTGGGGGCGGACGGATTGGGTGTGCCCGTGG
TGCGCGCGCTCAATCAGGTGAATACGGGCCTCGGATTTGAGAGCGGATTTATCATCGTCGTCGTGGCCATCGTGCTGGAC
CGAACCTTGAGGGTGAAAACATGA

Upstream 100 bases:

>100_bases
GCGAAGCGGTTCGCCCCCGTGAAGCGCAAGCCGTTGGATCGGCCCTTTAATATCCCGCCCCGGCACGGCATGCTGGGGCC
GGACTGTAAGGGGCACGGAC

Downstream 100 bases:

>100_bases
AGCTGAACGGTCAATGTCATTGTGGCGCGGTGCGCTTTACGGCTGAATTGAAGGGCGGGTTGGCGTCCGCGCGGCGCTGC
GATTGCTCGCTGTGCACGAT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MWWQNFRQWWRDDGISECSFPNDPDVTGPITFLTECKLRIGDGAEAVFDWLQSNGAFIFDAMAVALEAMIEVFLAILQEP
PNTSWFQDIRLRDAAFLYPQDWHPFLVIAIFTALAWWMHRGWKVPMLVAVGFLFIVNQDYWEETTESLTLVLSACVVCMG
VGVPIGIAVAHRPRLYAWMRPVLDLMQTLPTFVYLIPAIVFFGIGMVPGLIATVIFVLPAPIRLTHLGISSTPQSLLEAA
DAFGATGSQKLWKVELPYALPQIMTGLNQTIMLSLSMVVIAALVGADGLGVPVVRALNQVNTGLGFESGFIIVVVAIVLD
RTLRVKT

Sequences:

>Translated_327_residues
MWWQNFRQWWRDDGISECSFPNDPDVTGPITFLTECKLRIGDGAEAVFDWLQSNGAFIFDAMAVALEAMIEVFLAILQEP
PNTSWFQDIRLRDAAFLYPQDWHPFLVIAIFTALAWWMHRGWKVPMLVAVGFLFIVNQDYWEETTESLTLVLSACVVCMG
VGVPIGIAVAHRPRLYAWMRPVLDLMQTLPTFVYLIPAIVFFGIGMVPGLIATVIFVLPAPIRLTHLGISSTPQSLLEAA
DAFGATGSQKLWKVELPYALPQIMTGLNQTIMLSLSMVVIAALVGADGLGVPVVRALNQVNTGLGFESGFIIVVVAIVLD
RTLRVKT
>Mature_327_residues
MWWQNFRQWWRDDGISECSFPNDPDVTGPITFLTECKLRIGDGAEAVFDWLQSNGAFIFDAMAVALEAMIEVFLAILQEP
PNTSWFQDIRLRDAAFLYPQDWHPFLVIAIFTALAWWMHRGWKVPMLVAVGFLFIVNQDYWEETTESLTLVLSACVVCMG
VGVPIGIAVAHRPRLYAWMRPVLDLMQTLPTFVYLIPAIVFFGIGMVPGLIATVIFVLPAPIRLTHLGISSTPQSLLEAA
DAFGATGSQKLWKVELPYALPQIMTGLNQTIMLSLSMVVIAALVGADGLGVPVVRALNQVNTGLGFESGFIIVVVAIVLD
RTLRVKT

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=218, Percent_Identity=45.8715596330275, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788449, Length=229, Percent_Identity=31.0043668122271, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=29.8507462686567, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786564, Length=157, Percent_Identity=32.484076433121, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36270; Mature: 36270

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWWQNFRQWWRDDGISECSFPNDPDVTGPITFLTECKLRIGDGAEAVFDWLQSNGAFIFD
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
AMAVALEAMIEVFLAILQEPPNTSWFQDIRLRDAAFLYPQDWHPFLVIAIFTALAWWMHR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHC
GWKVPMLVAVGFLFIVNQDYWEETTESLTLVLSACVVCMGVGVPIGIAVAHRPRLYAWMR
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
PVLDLMQTLPTFVYLIPAIVFFGIGMVPGLIATVIFVLPAPIRLTHLGISSTPQSLLEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
DAFGATGSQKLWKVELPYALPQIMTGLNQTIMLSLSMVVIAALVGADGLGVPVVRALNQV
HHHCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NTGLGFESGFIIVVVAIVLDRTLRVKT
HCCCCCCCCHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MWWQNFRQWWRDDGISECSFPNDPDVTGPITFLTECKLRIGDGAEAVFDWLQSNGAFIFD
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
AMAVALEAMIEVFLAILQEPPNTSWFQDIRLRDAAFLYPQDWHPFLVIAIFTALAWWMHR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHC
GWKVPMLVAVGFLFIVNQDYWEETTESLTLVLSACVVCMGVGVPIGIAVAHRPRLYAWMR
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
PVLDLMQTLPTFVYLIPAIVFFGIGMVPGLIATVIFVLPAPIRLTHLGISSTPQSLLEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
DAFGATGSQKLWKVELPYALPQIMTGLNQTIMLSLSMVVIAALVGADGLGVPVVRALNQV
HHHCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NTGLGFESGFIIVVVAIVLDRTLRVKT
HCCCCCCCCHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]