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The map label for this gene is linC [H]

Identifier: 89053949

GI number: 89053949

Start: 1427403

End: 1428125

Strand: Direct

Name: linC [H]

Synonym: Jann_1458

Alternate gene names: 89053949

Gene position: 1427403-1428125 (Clockwise)

Preceding gene: 89053948

Following gene: 89053951

Centisome position: 33.06

GC content: 64.59

Gene sequence:

>723_bases
GTGAGCGATCTTGCGGGCAAGGTGATCCTGGTGACCGGTGCCGCGCGCGGAATAGGCGCGGCGATTGCCTCGGCCTGTGT
GGATGCAGGGGCGGAGGTGATTGGGGTAGATCGCGACCCGGTGCCCGGTGGGATTTTGTCGGATCTATCGCAAGCCGCCG
CCCCGGAGGCGGTTGTGGCGGAGGCGCTGGACCGTTCTGGTCGGCTCGACGGGCTTGTGAATGCGGCAGGCGTGACCACC
CGCGCGTCTTTTCTGGACGCGGATGTTGCGCGGTTCGATCAGGTCATGGCCGTGAACCTGCGTGCGCCGTTCTTCCTGAT
GGCCGGGGTGATTGCACACCTTCGGGGGCGCGATGCGCCAGGATCCATCGTAAACATCCAGTCAATGAACGCCCATTGCG
GCGTGCCGGATCTGGCGATTTATGCTGCCAGCAAAGGCGGGTTGCAAACGCTGACGAGGAACGCGGCGCAGACCCATATG
GCGGACCGTATTCGGGTGAACGGCATCAACCTTGGCTGGGTCGCGACCGAGACCGAGCGTGCAGTTCATGGTGACAAAGG
CGTGCGTTGGCTGGACGGCGAGGCGGCCAGACAGCCATTGGGACGCTTCGTGACAGACGTGGAATGCGCGGCGCAGGCCG
TCTGGATGTTGTCCGATGCCGCCCGCCCGATGAGCGGTGTGTGTCTTGATCTGGAGCAATGGATCGCAGGCGCACCGCCC
TAG

Upstream 100 bases:

>100_bases
GGTTCAATTACAAGACCGGCGCTGCCCTGCGCACGCCCGCCTGTGTGGCCTTGGCGACCTATCCGGCCCGGGTTGAGGGC
GGGCGGATCCAGGTTCAGGT

Downstream 100 bases:

>100_bases
CGCGGTGGCGCGGTAGACGCGCGCACGACCAGGTCCACCGACAACAGATCTTCCGGCGGCAGGGGCGCTTGCGGCGCGCT
GATCCGCTCCAACAGACGCG

Product: short chain dehydrogenase

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSDLAGKVILVTGAARGIGAAIASACVDAGAEVIGVDRDPVPGGILSDLSQAAAPEAVVAEALDRSGRLDGLVNAAGVTT
RASFLDADVARFDQVMAVNLRAPFFLMAGVIAHLRGRDAPGSIVNIQSMNAHCGVPDLAIYAASKGGLQTLTRNAAQTHM
ADRIRVNGINLGWVATETERAVHGDKGVRWLDGEAARQPLGRFVTDVECAAQAVWMLSDAARPMSGVCLDLEQWIAGAPP

Sequences:

>Translated_240_residues
MSDLAGKVILVTGAARGIGAAIASACVDAGAEVIGVDRDPVPGGILSDLSQAAAPEAVVAEALDRSGRLDGLVNAAGVTT
RASFLDADVARFDQVMAVNLRAPFFLMAGVIAHLRGRDAPGSIVNIQSMNAHCGVPDLAIYAASKGGLQTLTRNAAQTHM
ADRIRVNGINLGWVATETERAVHGDKGVRWLDGEAARQPLGRFVTDVECAAQAVWMLSDAARPMSGVCLDLEQWIAGAPP
>Mature_239_residues
SDLAGKVILVTGAARGIGAAIASACVDAGAEVIGVDRDPVPGGILSDLSQAAAPEAVVAEALDRSGRLDGLVNAAGVTTR
ASFLDADVARFDQVMAVNLRAPFFLMAGVIAHLRGRDAPGSIVNIQSMNAHCGVPDLAIYAASKGGLQTLTRNAAQTHMA
DRIRVNGINLGWVATETERAVHGDKGVRWLDGEAARQPLGRFVTDVECAAQAVWMLSDAARPMSGVCLDLEQWIAGAPP

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=243, Percent_Identity=34.156378600823, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI5031737, Length=239, Percent_Identity=29.7071129707113, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI33667109, Length=196, Percent_Identity=31.1224489795918, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI59889578, Length=228, Percent_Identity=29.8245614035088, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19923817, Length=250, Percent_Identity=25.6, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI32483357, Length=239, Percent_Identity=28.4518828451883, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790717, Length=245, Percent_Identity=32.6530612244898, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=27.710843373494, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786812, Length=241, Percent_Identity=29.4605809128631, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787335, Length=242, Percent_Identity=30.1652892561983, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788459, Length=199, Percent_Identity=29.6482412060301, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082160, Length=246, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082100, Length=245, Percent_Identity=27.7551020408163, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=29.0983606557377, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=244, Percent_Identity=31.1475409836066, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=187, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=231, Percent_Identity=26.4069264069264, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=204, Percent_Identity=32.843137254902, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=243, Percent_Identity=27.5720164609054, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=248, Percent_Identity=27.8225806451613, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=204, Percent_Identity=32.843137254902, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=258, Percent_Identity=28.2945736434109, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=242, Percent_Identity=30.1652892561983, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=28.6259541984733, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=200, Percent_Identity=30, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=243, Percent_Identity=26.3374485596708, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=249, Percent_Identity=26.9076305220884, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=200, Percent_Identity=27, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI23397609, Length=239, Percent_Identity=32.2175732217573, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643142, Length=250, Percent_Identity=32, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28571526, Length=250, Percent_Identity=26.8, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644337, Length=248, Percent_Identity=31.4516129032258, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639444, Length=243, Percent_Identity=31.2757201646091, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355319, Length=242, Percent_Identity=26.8595041322314, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 24832; Mature: 24700

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLAGKVILVTGAARGIGAAIASACVDAGAEVIGVDRDPVPGGILSDLSQAAAPEAVVA
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHH
EALDRSGRLDGLVNAAGVTTRASFLDADVARFDQVMAVNLRAPFFLMAGVIAHLRGRDAP
HHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCCC
GSIVNIQSMNAHCGVPDLAIYAASKGGLQTLTRNAAQTHMADRIRVNGINLGWVATETER
CCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCEEEEEEECCCHH
AVHGDKGVRWLDGEAARQPLGRFVTDVECAAQAVWMLSDAARPMSGVCLDLEQWIAGAPP
HHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHCCCCC
>Mature Secondary Structure 
SDLAGKVILVTGAARGIGAAIASACVDAGAEVIGVDRDPVPGGILSDLSQAAAPEAVVA
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHH
EALDRSGRLDGLVNAAGVTTRASFLDADVARFDQVMAVNLRAPFFLMAGVIAHLRGRDAP
HHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHCCCCCC
GSIVNIQSMNAHCGVPDLAIYAASKGGLQTLTRNAAQTHMADRIRVNGINLGWVATETER
CCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCEEEEEEECCCHH
AVHGDKGVRWLDGEAARQPLGRFVTDVECAAQAVWMLSDAARPMSGVCLDLEQWIAGAPP
HHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]