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The map label for this gene is maiA [H]

Identifier: 89053928

GI number: 89053928

Start: 1405207

End: 1405839

Strand: Reverse

Name: maiA [H]

Synonym: Jann_1437

Alternate gene names: 89053928

Gene position: 1405839-1405207 (Counterclockwise)

Preceding gene: 89053929

Following gene: 89053927

Centisome position: 32.56

GC content: 66.98

Gene sequence:

>633_bases
ATGATCCTTTATTCCTACTGGCGATCGACCACATCTTACCGTGTCCGCGCGGCGCTGAACCTCAAGGGCATCGCCTATGA
GCAGCGCCCGGTGGACCTCGTGGCAGGCGATCAGCGCGCGCCCGACTACGCCGTTCTCAACCCCGGCCTTGGCGTCCCGG
CCCTCACCCTGGACGACGGCACAACCCTGACCCAAAGCCTCGCGATCATCGACTACCTCGATGCGACCTACCCAGAGCCG
TCTCTCCTGCCCGCTGACCCGCTCCTGCGCGCCCGCGTCCTCGCCGCGGCCCACACCGTCGCGCTCGACATCCACCCGGT
GAACAACCTGCGCGTCATCGGCCGCCTGAAATCCCACCATGCCGCAACCCCGGAGGACGCCCAGATCTGGATGCGCCACT
GGATGGCCGAGGGCTTCACCGCCTTGGAAACCCTACTGCCGGACCGTGCCACCTTTGCCTTCACCGATACGCCGACCCTT
GCCGACCTCTGCATTGTCGCGCAATGCTACAACGCCCATCGCTGGGGTCTGCCGCTGACACCCTTCCCGCACATCCAACG
CATCGAGGCCGCCTGCCGCGCCACCCCCGAAATCGCCGCCGCCGCACCAGAGGCGCAACCCGACGCACCCTAA

Upstream 100 bases:

>100_bases
CCGTGTGGTCTGATCCCGATACCGAAAAGCTGATGGGTGATGGTCTCGTGGATGGTCAGCGGATGATCATGGGCGGGTTT
GAGATCCTTCTGGACGTCAC

Downstream 100 bases:

>100_bases
CCCGCCCGTCCTCTTGCCCAAAACGCGCCCCGCCGTCCCCGGATATGGGGCGCCGATCCGCCACACGCCCTAACGAAAGC
CCGACATGTCCGACCTGATC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MILYSYWRSTTSYRVRAALNLKGIAYEQRPVDLVAGDQRAPDYAVLNPGLGVPALTLDDGTTLTQSLAIIDYLDATYPEP
SLLPADPLLRARVLAAAHTVALDIHPVNNLRVIGRLKSHHAATPEDAQIWMRHWMAEGFTALETLLPDRATFAFTDTPTL
ADLCIVAQCYNAHRWGLPLTPFPHIQRIEAACRATPEIAAAAPEAQPDAP

Sequences:

>Translated_210_residues
MILYSYWRSTTSYRVRAALNLKGIAYEQRPVDLVAGDQRAPDYAVLNPGLGVPALTLDDGTTLTQSLAIIDYLDATYPEP
SLLPADPLLRARVLAAAHTVALDIHPVNNLRVIGRLKSHHAATPEDAQIWMRHWMAEGFTALETLLPDRATFAFTDTPTL
ADLCIVAQCYNAHRWGLPLTPFPHIQRIEAACRATPEIAAAAPEAQPDAP
>Mature_210_residues
MILYSYWRSTTSYRVRAALNLKGIAYEQRPVDLVAGDQRAPDYAVLNPGLGVPALTLDDGTTLTQSLAIIDYLDATYPEP
SLLPADPLLRARVLAAAHTVALDIHPVNNLRVIGRLKSHHAATPEDAQIWMRHWMAEGFTALETLLPDRATFAFTDTPTL
ADLCIVAQCYNAHRWGLPLTPFPHIQRIEAACRATPEIAAAAPEAQPDAP

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=41.5094339622642, Blast_Score=150, Evalue=9e-37,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=38.125, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=40.3846153846154, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17556142, Length=211, Percent_Identity=40.7582938388626, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17510461, Length=211, Percent_Identity=37.4407582938389, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24645375, Length=212, Percent_Identity=40.0943396226415, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21355859, Length=212, Percent_Identity=40.0943396226415, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45553325, Length=212, Percent_Identity=40.0943396226415, Blast_Score=153, Evalue=8e-38,
Organism=Drosophila melanogaster, GI21355857, Length=213, Percent_Identity=38.9671361502347, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23012; Mature: 23012

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYSYWRSTTSYRVRAALNLKGIAYEQRPVDLVAGDQRAPDYAVLNPGLGVPALTLDDG
CEEEEEECCCCCEEEEEEEEECCEEECCCCCEEEECCCCCCCEEEECCCCCCCEEEECCC
TTLTQSLAIIDYLDATYPEPSLLPADPLLRARVLAAAHTVALDIHPVNNLRVIGRLKSHH
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCEEEEEEHHHCC
AATPEDAQIWMRHWMAEGFTALETLLPDRATFAFTDTPTLADLCIVAQCYNAHRWGLPLT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCC
PFPHIQRIEAACRATPEIAAAAPEAQPDAP
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MILYSYWRSTTSYRVRAALNLKGIAYEQRPVDLVAGDQRAPDYAVLNPGLGVPALTLDDG
CEEEEEECCCCCEEEEEEEEECCEEECCCCCEEEECCCCCCCEEEECCCCCCCEEEECCC
TTLTQSLAIIDYLDATYPEPSLLPADPLLRARVLAAAHTVALDIHPVNNLRVIGRLKSHH
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCEEEEEEHHHCC
AATPEDAQIWMRHWMAEGFTALETLLPDRATFAFTDTPTLADLCIVAQCYNAHRWGLPLT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCC
PFPHIQRIEAACRATPEIAAAAPEAQPDAP
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]