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The map label for this gene is yafV [C]

Identifier: 89053569

GI number: 89053569

Start: 1040333

End: 1041229

Strand: Reverse

Name: yafV [C]

Synonym: Jann_1078

Alternate gene names: 89053569

Gene position: 1041229-1040333 (Counterclockwise)

Preceding gene: 89053571

Following gene: 89053568

Centisome position: 24.11

GC content: 64.99

Gene sequence:

>897_bases
ATGACCGATACGTTGCGCGTTTCGATGGTTCAGATGACCTCGACGAATTCTCACTCGGACAATGTTCGGAGCCTTCGGCA
AGCGGCGCAGCAGGCGGCCGATCAGAATGCGGACATGCTGGCGCTGCCGGAAGCGGCCGGCCTCATGGATCGCGACAAGG
ATCACGCCCGCGCCCAGATTACCGGGGAAGGGGGTGATCCCTACATCACGGCCTGCCGGGAAGAGGCCGCGCGCCATGGC
ATCTGGGTCCATTCCGGGTCCTGCCCGGTCAAAGCGCCCGACGGGCGATACCTCAACCACACTGTTCTGGTGGCCCCCTC
CGGCGACATCGTCGCGCGCTACGACAAGATCCACCTGTTCGACGTGTTTCTGGACGGGCGCAGGGCAACGGGTGAATCGG
ACAGATATGCGCCAGGCTCCGAAGCCGTGGTGGCGGATACGCCGTTTGGCCCCATGGCGCTGTCGATCTGCTATGACCTT
CGGTTCCCGCACCTGTACCGCGACTATGCATTGGCCGGGTCCACGGTGATGTTCATTCCCTCCGCCTTCACCGTGCCCAC
GGGACGGGCCCATTGGGAGGTCTTGCTGCGCGCCCGCGCGATCGAGAACGGCGCCTATGTGATTGCAGCCGCGCAGGTGG
GTCACCACGCGGACGGGCGCGTGACGTGGGGCCATTCCCTGATCGTGTCGCCCTGGGGTGACGTGCTGGCCGATCTGGGG
GGAGACGCGCCGGGCCAGATCACGCTGGACCTGCCGCTGGGCGATGTCGTCGCCGCGCGTCGGCAGATCCCAAACCTGCA
AAACGCCCGCGATTACACGTTCCGCCACCTCAAGGGGGACGGGACCCACACGACGTCACCACAACACCAAGATACATCCA
CGGGAGCTATGACATGA

Upstream 100 bases:

>100_bases
CGACCTGTGAGAAATTCAGACAGATTGCAACAATTTTGTTCTTTTTTCTTGAGGGTTTCGCACCCTATTGTTGGGGGTCG
CACCAATCAAAGGGTTGAAA

Downstream 100 bases:

>100_bases
CTTTACGCAACCTGATGGCCATCACGCTTCTTGCCGGCTCGCTGGCGCTGCCTGCCGCAGCCCAGGAGCGCCCGGATCTG
GTCATCGCCGTCGACAACCT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTDTLRVSMVQMTSTNSHSDNVRSLRQAAQQAADQNADMLALPEAAGLMDRDKDHARAQITGEGGDPYITACREEAARHG
IWVHSGSCPVKAPDGRYLNHTVLVAPSGDIVARYDKIHLFDVFLDGRRATGESDRYAPGSEAVVADTPFGPMALSICYDL
RFPHLYRDYALAGSTVMFIPSAFTVPTGRAHWEVLLRARAIENGAYVIAAAQVGHHADGRVTWGHSLIVSPWGDVLADLG
GDAPGQITLDLPLGDVVAARRQIPNLQNARDYTFRHLKGDGTHTTSPQHQDTSTGAMT

Sequences:

>Translated_298_residues
MTDTLRVSMVQMTSTNSHSDNVRSLRQAAQQAADQNADMLALPEAAGLMDRDKDHARAQITGEGGDPYITACREEAARHG
IWVHSGSCPVKAPDGRYLNHTVLVAPSGDIVARYDKIHLFDVFLDGRRATGESDRYAPGSEAVVADTPFGPMALSICYDL
RFPHLYRDYALAGSTVMFIPSAFTVPTGRAHWEVLLRARAIENGAYVIAAAQVGHHADGRVTWGHSLIVSPWGDVLADLG
GDAPGQITLDLPLGDVVAARRQIPNLQNARDYTFRHLKGDGTHTTSPQHQDTSTGAMT
>Mature_297_residues
TDTLRVSMVQMTSTNSHSDNVRSLRQAAQQAADQNADMLALPEAAGLMDRDKDHARAQITGEGGDPYITACREEAARHGI
WVHSGSCPVKAPDGRYLNHTVLVAPSGDIVARYDKIHLFDVFLDGRRATGESDRYAPGSEAVVADTPFGPMALSICYDLR
FPHLYRDYALAGSTVMFIPSAFTVPTGRAHWEVLLRARAIENGAYVIAAAQVGHHADGRVTWGHSLIVSPWGDVLADLGG
DAPGQITLDLPLGDVVAARRQIPNLQNARDYTFRHLKGDGTHTTSPQHQDTSTGAMT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=272, Percent_Identity=37.1323529411765, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI297632350, Length=272, Percent_Identity=37.1323529411765, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI5031947, Length=272, Percent_Identity=37.1323529411765, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI9910460, Length=270, Percent_Identity=34.0740740740741, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=33.8461538461538, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786412, Length=192, Percent_Identity=31.7708333333333, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=234, Percent_Identity=38.034188034188, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322335, Length=307, Percent_Identity=28.6644951140065, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=27.3049645390071, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17933642, Length=267, Percent_Identity=37.0786516853933, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21355835, Length=265, Percent_Identity=26.4150943396226, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 32235; Mature: 32104

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTLRVSMVQMTSTNSHSDNVRSLRQAAQQAADQNADMLALPEAAGLMDRDKDHARAQI
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCCCCCCEEEE
TGEGGDPYITACREEAARHGIWVHSGSCPVKAPDGRYLNHTVLVAPSGDIVARYDKIHLF
ECCCCCCEEHHHHHHHHHCCEEEECCCCCCCCCCCCEECEEEEEECCCCEEEEECEEEEE
DVFLDGRRATGESDRYAPGSEAVVADTPFGPMALSICYDLRFPHLYRDYALAGSTVMFIP
EEEECCCCCCCCCCCCCCCCCEEEEECCCCHHEEEEHHHCCCCHHHHHHHHCCCEEEEEC
SAFTVPTGRAHWEVLLRARAIENGAYVIAAAQVGHHADGRVTWGHSLIVSPWGDVLADLG
CEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCEEEECCHHHHHHHHC
GDAPGQITLDLPLGDVVAARRQIPNLQNARDYTFRHLKGDGTHTTSPQHQDTSTGAMT
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTLRVSMVQMTSTNSHSDNVRSLRQAAQQAADQNADMLALPEAAGLMDRDKDHARAQI
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCCCCCCEEEE
TGEGGDPYITACREEAARHGIWVHSGSCPVKAPDGRYLNHTVLVAPSGDIVARYDKIHLF
ECCCCCCEEHHHHHHHHHCCEEEECCCCCCCCCCCCEECEEEEEECCCCEEEEECEEEEE
DVFLDGRRATGESDRYAPGSEAVVADTPFGPMALSICYDLRFPHLYRDYALAGSTVMFIP
EEEECCCCCCCCCCCCCCCCCEEEEECCCCHHEEEEHHHCCCCHHHHHHHHCCCEEEEEC
SAFTVPTGRAHWEVLLRARAIENGAYVIAAAQVGHHADGRVTWGHSLIVSPWGDVLADLG
CEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCEEEECCHHHHHHHHC
GDAPGQITLDLPLGDVVAARRQIPNLQNARDYTFRHLKGDGTHTTSPQHQDTSTGAMT
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]