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The map label for this gene is mcl2 [H]

Identifier: 89053307

GI number: 89053307

Start: 773991

End: 774854

Strand: Reverse

Name: mcl2 [H]

Synonym: Jann_0816

Alternate gene names: 89053307

Gene position: 774854-773991 (Counterclockwise)

Preceding gene: 89053329

Following gene: 89053306

Centisome position: 17.94

GC content: 64.47

Gene sequence:

>864_bases
ATGCCCATGTCTCAGCCCTGCCGATCCGTCCTCTATATCCCCGCCTCCCGCGCCCGCGCGATGGAGAAGGCCAGGACGCT
GCCGGTCGACGCGATAATCTTCGACCTGGAAGATGCCGTCGTCGCCGACGAAAAGGTCGCCGCGCGCGACACCCTGATCG
GCACCCTGAACGATGGCGACTTCGGCCCGCGCCAACAGATCATCCGCATCAACGGCTTTGACACCCAATGGGGGGCCGAC
GACCTGAACGCCATCGCGCGGGCCGCGCCCCAGGCCGTTCTTCTGCCCAAGGTCGACGGCGCCAGCGACATTGAGGCGCT
CGCGGTCCTGCTAGACGCCCACCCGCAGGCAAAAGACACCCGGATCTGGGCGATGATGGAAACGCCTCTGGGCATCCTGA
ACGCCGCCGAAATCGCGTGCGCGCCCCGCATGGCTGGCTTTGTGATGGGCACCAATGACCTCGCCAAGGATCTGACCGCG
CGCACCCGCGATGCGATGCAGACCTCCCTGCAACTCTGCGTTCTGGCGGCCCGCGCCCATGGCATTCTCTGCGTTGACGG
TGTCTATAATGCCTTCAAGGACGACGATGGTCTGCGGGCCGAATGCCAGCAGGGACGTGACTTTGGCTTTGACGGCAAAA
CGCTGATCCACCCGGCGCAGATTGCCATCGCCAACGCCACCTTCGGCCCGACAGAGCAGGATATCGCACTGGCCCGCCGC
CAGATTGAGGCCTATGAGGCCGCGCTGACCAAAGGCGAAGGCGTCGCCGTTGTGGATGGTAAGATTGTCGAGAACCTGCA
CGTTGAAACCGCCCGCGCCACCCTGGCGAAATCAGAGGCCATCGCCGCATTGGAGACCGCATGA

Upstream 100 bases:

>100_bases
CCCGCGAACTTTTGTTGCGCGTCATTCTGCGCTGCAGCAAGACCCTTGCGCTGGCGGCGATCATGTGGTCCCAAAAGGGT
TGAACATAGCCACAGGCCAA

Downstream 100 bases:

>100_bases
TATCCTGGATCCTACTTATCGCGGGGCTCGCCCTGTGGGTCGCAGCGCACGCCTGGAAACGAGTCGCGCCCGCGCATCGG
GCCAGCTTCGGGGACAAGGG

Product: Citryl-CoA lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPMSQPCRSVLYIPASRARAMEKARTLPVDAIIFDLEDAVVADEKVAARDTLIGTLNDGDFGPRQQIIRINGFDTQWGAD
DLNAIARAAPQAVLLPKVDGASDIEALAVLLDAHPQAKDTRIWAMMETPLGILNAAEIACAPRMAGFVMGTNDLAKDLTA
RTRDAMQTSLQLCVLAARAHGILCVDGVYNAFKDDDGLRAECQQGRDFGFDGKTLIHPAQIAIANATFGPTEQDIALARR
QIEAYEAALTKGEGVAVVDGKIVENLHVETARATLAKSEAIAALETA

Sequences:

>Translated_287_residues
MPMSQPCRSVLYIPASRARAMEKARTLPVDAIIFDLEDAVVADEKVAARDTLIGTLNDGDFGPRQQIIRINGFDTQWGAD
DLNAIARAAPQAVLLPKVDGASDIEALAVLLDAHPQAKDTRIWAMMETPLGILNAAEIACAPRMAGFVMGTNDLAKDLTA
RTRDAMQTSLQLCVLAARAHGILCVDGVYNAFKDDDGLRAECQQGRDFGFDGKTLIHPAQIAIANATFGPTEQDIALARR
QIEAYEAALTKGEGVAVVDGKIVENLHVETARATLAKSEAIAALETA
>Mature_286_residues
PMSQPCRSVLYIPASRARAMEKARTLPVDAIIFDLEDAVVADEKVAARDTLIGTLNDGDFGPRQQIIRINGFDTQWGADD
LNAIARAAPQAVLLPKVDGASDIEALAVLLDAHPQAKDTRIWAMMETPLGILNAAEIACAPRMAGFVMGTNDLAKDLTAR
TRDAMQTSLQLCVLAARAHGILCVDGVYNAFKDDDGLRAECQQGRDFGFDGKTLIHPAQIAIANATFGPTEQDIALARRQ
IEAYEAALTKGEGVAVVDGKIVENLHVETARATLAKSEAIAALETA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=294, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI87081764, Length=281, Percent_Identity=34.8754448398576, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17557458, Length=282, Percent_Identity=27.3049645390071, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30663; Mature: 30532

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSQPCRSVLYIPASRARAMEKARTLPVDAIIFDLEDAVVADEKVAARDTLIGTLNDGD
CCCCCCCHHEEECCCHHHHHHHHHHCCCHHEEEEECCHHHHCCCHHHHHHHEEEECCCCC
FGPRQQIIRINGFDTQWGADDLNAIARAAPQAVLLPKVDGASDIEALAVLLDAHPQAKDT
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
RIWAMMETPLGILNAAEIACAPRMAGFVMGTNDLAKDLTARTRDAMQTSLQLCVLAARAH
EEEEEECCCCCHHHHHHHHHCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GILCVDGVYNAFKDDDGLRAECQQGRDFGFDGKTLIHPAQIAIANATFGPTEQDIALARR
CEEEEHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHH
QIEAYEAALTKGEGVAVVDGKIVENLHVETARATLAKSEAIAALETA
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PMSQPCRSVLYIPASRARAMEKARTLPVDAIIFDLEDAVVADEKVAARDTLIGTLNDGD
CCCCCCHHEEECCCHHHHHHHHHHCCCHHEEEEECCHHHHCCCHHHHHHHEEEECCCCC
FGPRQQIIRINGFDTQWGADDLNAIARAAPQAVLLPKVDGASDIEALAVLLDAHPQAKDT
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
RIWAMMETPLGILNAAEIACAPRMAGFVMGTNDLAKDLTARTRDAMQTSLQLCVLAARAH
EEEEEECCCCCHHHHHHHHHCHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GILCVDGVYNAFKDDDGLRAECQQGRDFGFDGKTLIHPAQIAIANATFGPTEQDIALARR
CEEEEHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHH
QIEAYEAALTKGEGVAVVDGKIVENLHVETARATLAKSEAIAALETA
HHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA