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The map label for this gene is mcbR [H]

Identifier: 89053329

GI number: 89053329

Start: 793841

End: 794494

Strand: Reverse

Name: mcbR [H]

Synonym: Jann_0838

Alternate gene names: 89053329

Gene position: 794494-793841 (Counterclockwise)

Preceding gene: 89053330

Following gene: 89053307

Centisome position: 18.4

GC content: 67.28

Gene sequence:

>654_bases
ATGCTGATGAACCCCCGCCCCACGGAAACCGGCGCCGCCGTGGCCCATGACCGGGTCTACCGCGCATTGCGCTCTCGCAT
CATGTACGGCGAGGTTGATCCGGGTCAGGCAATGACGTTGCGCGGCGTGGGGCGCGAGTTTGGCGTGTCGATGACGCCTG
CGCGGGAGGCGGTGCGCCGCCTGGTCAGCGAGGGTGCGCTGTTCCTGTCAGCGTCGGGCCGGGTCTCGACGCCGGAACTG
TCGAACGAGCGGATTGAAGAGCTGGCGTCCCTGCGTGCCTTGCTGGAGCCGGAATTGGCCTCCCGCGCCCTGCCCCGTGC
GCATCCCGCGCTGATTGAACGGATGGAGGCGATCAACGCACAAATCGCGCAGGTCATCGCGAAGCGGGATGCGGTGGCCT
ACATCAAGTCAAATCTGGAATTCCACAGGACGCTGTATCTGCGCGCCCAGGCCCCGGCGATGCTGGCGCTGGCAGAGACG
GTCTGGCTGCAAATGGGCCCCACCATGCGCGCCCTCTACGGTCGGTTGAAACGCACCGAATCGCCGCCGCATCACCGGCT
GATCCTCGCGGCGCTGCGCGCGGGAGATGAGCCGGGACTGCGGCTGGCCCTGCGGGCTGACGTCACACAGGGCCTGCGAC
TTTTGGCGAACTAG

Upstream 100 bases:

>100_bases
TGCAAACAATTGTGCCCGACCACAGACCAGCACCGCCGCTGGATGCGCGCCCACGGGGCGGAGCTTCTGGCCTGGCGGTT
TGAGGCGCAGGAGTGATGCC

Downstream 100 bases:

>100_bases
GATTGCGCCGCGCGCCGTCAGGCGTGGATCGCGCCGTCGCCGCAGGCAAGTGCTGCTTCGCGCACGGCCTCCGAATACGT
TGGGTGGGCGTGGCACGTCA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MLMNPRPTETGAAVAHDRVYRALRSRIMYGEVDPGQAMTLRGVGREFGVSMTPAREAVRRLVSEGALFLSASGRVSTPEL
SNERIEELASLRALLEPELASRALPRAHPALIERMEAINAQIAQVIAKRDAVAYIKSNLEFHRTLYLRAQAPAMLALAET
VWLQMGPTMRALYGRLKRTESPPHHRLILAALRAGDEPGLRLALRADVTQGLRLLAN

Sequences:

>Translated_217_residues
MLMNPRPTETGAAVAHDRVYRALRSRIMYGEVDPGQAMTLRGVGREFGVSMTPAREAVRRLVSEGALFLSASGRVSTPEL
SNERIEELASLRALLEPELASRALPRAHPALIERMEAINAQIAQVIAKRDAVAYIKSNLEFHRTLYLRAQAPAMLALAET
VWLQMGPTMRALYGRLKRTESPPHHRLILAALRAGDEPGLRLALRADVTQGLRLLAN
>Mature_217_residues
MLMNPRPTETGAAVAHDRVYRALRSRIMYGEVDPGQAMTLRGVGREFGVSMTPAREAVRRLVSEGALFLSASGRVSTPEL
SNERIEELASLRALLEPELASRALPRAHPALIERMEAINAQIAQVIAKRDAVAYIKSNLEFHRTLYLRAQAPAMLALAET
VWLQMGPTMRALYGRLKRTESPPHHRLILAALRAGDEPGLRLALRADVTQGLRLLAN

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=201, Percent_Identity=27.8606965174129, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 23961; Mature: 23961

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMNPRPTETGAAVAHDRVYRALRSRIMYGEVDPGQAMTLRGVGREFGVSMTPAREAVRR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCHHHCCCCCHHHHHHHH
LVSEGALFLSASGRVSTPELSNERIEELASLRALLEPELASRALPRAHPALIERMEAINA
HHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHH
QIAQVIAKRDAVAYIKSNLEFHRTLYLRAQAPAMLALAETVWLQMGPTMRALYGRLKRTE
HHHHHHHHHHHHHHHHHCCCEEHEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
SPPHHRLILAALRAGDEPGLRLALRADVTQGLRLLAN
CCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MLMNPRPTETGAAVAHDRVYRALRSRIMYGEVDPGQAMTLRGVGREFGVSMTPAREAVRR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCHHHCCCCCHHHHHHHH
LVSEGALFLSASGRVSTPELSNERIEELASLRALLEPELASRALPRAHPALIERMEAINA
HHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHH
QIAQVIAKRDAVAYIKSNLEFHRTLYLRAQAPAMLALAETVWLQMGPTMRALYGRLKRTE
HHHHHHHHHHHHHHHHHCCCEEHEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
SPPHHRLILAALRAGDEPGLRLALRADVTQGLRLLAN
CCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]