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The map label for this gene is merA [H]

Identifier: 89053136

GI number: 89053136

Start: 615356

End: 616774

Strand: Direct

Name: merA [H]

Synonym: Jann_0645

Alternate gene names: 89053136

Gene position: 615356-616774 (Clockwise)

Preceding gene: 89053135

Following gene: 89053137

Centisome position: 14.25

GC content: 61.31

Gene sequence:

>1419_bases
ATGTCTACGATTGAAACTGATATCTGCGTCATCGGCGCGGGCTCTGGCGGTTTGTCCGTTGCGGCGGGTGCTGTCCAAAT
GGGCGCGAAGGTGGTCCTTCTGGAGGGGCACCTGATGGGGGGCGATTGCCTGAACTTTGGCTGCGTCCCCTCCAAGGCGT
TGCTGGCGGCCGGTCACAAGGCGCATGAGACCAGCGAAGCCGCGTTTGGCGTTGCGGGGCATGAGCCGTCCCCGGATTAC
GCGGCGGCGAAAGATCACGTCCAAGCGGTGATCGACGAGATTGCCCCCGTCGATTCGCAGGAGCGGTTTGAGGGCCTTGG
CGTTCATGTGATCCGCGAATTTGGGCGGTTCATCTCGGAGTCTGAAGTTCAGGCGGGCGCGCATACAATCAAGGCGCGCC
GGTTCGTGATCGCCACCGGCTCTCGCCCCTTTGTGCCGCCGATCCCGGGGCTGGACACGGTGGAGTACCACACCAACGAG
ACCATCTTTGACCTGCGCGAGCGTCCCGATCATCTGATCATCATCGGTGGCGGCCCCATCGGGATGGAGATGGCACAGGC
CCATCGGCGGCTTGGGTCGCGCGTCACCGTGCTGGAAGGTGCCAAAGCCATGGGTAAGGATGACCCGGAGGCGGCCGCCA
TCGTGCTGGACAACCTGCGCGCGGAAGGGATCGAGATTGTGGAGGGCGCTTTGGCCTCCCAGATCAAGGGATCCGATGGG
TCGGTAACGGTCGAGACAAAGGATGGCGCGAGCTATGAGGGCTCCCACCTGCTGATGGCCGTGGGTCGTGCGGTCAATGT
CGATAAGCTGGATCTGGAGAAAGCGGGCGTGGAATATGACCGCTCCGGCGTGAAAGTGGGCGATGATCTGCGATCCACCA
ACAAGCGGGTTTATGCCGTGGGCGATGTGGCCGGTGGCGCGCAGTTCACCCATGTCGCGGGCTACCACGCAGGCGTTATC
ATCCGTCCGATGCTGTTCGGCCTGCCTGCCAAAGCACGCAAAGATCATATTCCATGGGCCACCTACACCTCGCCGGAACT
GGCGCAGGTCGGCCTGACGGAAGCAGAGGCGAAGGAGCAACACGGCGATAACGTCTTCATCGCGAAAGCGGAGTTCGAGC
ACAATGACCGGGGCATCGCCACCGGGCAAACCAAGGGCTTTGTCAAAGTCATGGTCGTTAAGGGAAAGCCCGTGGGTGCC
ACAATCGTCGGCCCCCAGGCCGGAGAGTTGATTGGCATTTGGTCGCTGGCCATTGCAAACAAGCTGAAGATGAGTGCCGT
GGCCAATATGATCGCCCCCTATCCGACATTGGGAGAGATCAACAAACGCGCTGCCAGTGCCTATTTCACCCCCAAGCTGT
TCGACAGTGCGCTGGTGAAAAAGGTCGTGCGGTTTGTGCAAAGGTTCGGTCGGCCTTAG

Upstream 100 bases:

>100_bases
TTTCGAGCCGCAGATCCTCCTGCCGATCCTCGGCCTCTGTGCTCTGGCGACGTTGCCGATTATCCTCAAGGCCGTGCGCG
GCAAGAAGGGCCTCTGATCC

Downstream 100 bases:

>100_bases
ATTAGGAGACAGCCCGGGCTCCGGGACCAAGAAGGAGCCGTTCGTGGCCAACTCACTCTCTGGGCGATTTCTGATCCTCA
CCATCATCTTCGTGATGCTG

Product: pyridine nucleotide-disulfide oxidoreductase dimerisation protein

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTIETDICVIGAGSGGLSVAAGAVQMGAKVVLLEGHLMGGDCLNFGCVPSKALLAAGHKAHETSEAAFGVAGHEPSPDY
AAAKDHVQAVIDEIAPVDSQERFEGLGVHVIREFGRFISESEVQAGAHTIKARRFVIATGSRPFVPPIPGLDTVEYHTNE
TIFDLRERPDHLIIIGGGPIGMEMAQAHRRLGSRVTVLEGAKAMGKDDPEAAAIVLDNLRAEGIEIVEGALASQIKGSDG
SVTVETKDGASYEGSHLLMAVGRAVNVDKLDLEKAGVEYDRSGVKVGDDLRSTNKRVYAVGDVAGGAQFTHVAGYHAGVI
IRPMLFGLPAKARKDHIPWATYTSPELAQVGLTEAEAKEQHGDNVFIAKAEFEHNDRGIATGQTKGFVKVMVVKGKPVGA
TIVGPQAGELIGIWSLAIANKLKMSAVANMIAPYPTLGEINKRAASAYFTPKLFDSALVKKVVRFVQRFGRP

Sequences:

>Translated_472_residues
MSTIETDICVIGAGSGGLSVAAGAVQMGAKVVLLEGHLMGGDCLNFGCVPSKALLAAGHKAHETSEAAFGVAGHEPSPDY
AAAKDHVQAVIDEIAPVDSQERFEGLGVHVIREFGRFISESEVQAGAHTIKARRFVIATGSRPFVPPIPGLDTVEYHTNE
TIFDLRERPDHLIIIGGGPIGMEMAQAHRRLGSRVTVLEGAKAMGKDDPEAAAIVLDNLRAEGIEIVEGALASQIKGSDG
SVTVETKDGASYEGSHLLMAVGRAVNVDKLDLEKAGVEYDRSGVKVGDDLRSTNKRVYAVGDVAGGAQFTHVAGYHAGVI
IRPMLFGLPAKARKDHIPWATYTSPELAQVGLTEAEAKEQHGDNVFIAKAEFEHNDRGIATGQTKGFVKVMVVKGKPVGA
TIVGPQAGELIGIWSLAIANKLKMSAVANMIAPYPTLGEINKRAASAYFTPKLFDSALVKKVVRFVQRFGRP
>Mature_471_residues
STIETDICVIGAGSGGLSVAAGAVQMGAKVVLLEGHLMGGDCLNFGCVPSKALLAAGHKAHETSEAAFGVAGHEPSPDYA
AAKDHVQAVIDEIAPVDSQERFEGLGVHVIREFGRFISESEVQAGAHTIKARRFVIATGSRPFVPPIPGLDTVEYHTNET
IFDLRERPDHLIIIGGGPIGMEMAQAHRRLGSRVTVLEGAKAMGKDDPEAAAIVLDNLRAEGIEIVEGALASQIKGSDGS
VTVETKDGASYEGSHLLMAVGRAVNVDKLDLEKAGVEYDRSGVKVGDDLRSTNKRVYAVGDVAGGAQFTHVAGYHAGVII
RPMLFGLPAKARKDHIPWATYTSPELAQVGLTEAEAKEQHGDNVFIAKAEFEHNDRGIATGQTKGFVKVMVVKGKPVGAT
IVGPQAGELIGIWSLAIANKLKMSAVANMIAPYPTLGEINKRAASAYFTPKLFDSALVKKVVRFVQRFGRP

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=31.3432835820896, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=28.1318681318681, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI22035672, Length=438, Percent_Identity=25.7990867579909, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI291045266, Length=433, Percent_Identity=26.3279445727483, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=24.4493392070485, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=24.4493392070485, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=24.4493392070485, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=24.4493392070485, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=24.4493392070485, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=25.0585480093677, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=31.9654427645788, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=30.5747126436782, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87081717, Length=442, Percent_Identity=28.9592760180996, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=27.0742358078603, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789065, Length=203, Percent_Identity=28.0788177339901, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=30.3347280334728, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=29.3248945147679, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=28.0334728033473, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=385, Percent_Identity=27.5324675324675, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=385, Percent_Identity=27.5324675324675, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=31.25, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=26.9807280513919, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=25.0517598343685, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358499, Length=480, Percent_Identity=32.2916666666667, Blast_Score=168, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=28.2426778242678, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=28.2426778242678, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=28.2426778242678, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=24.468085106383, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49908; Mature: 49776

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIETDICVIGAGSGGLSVAAGAVQMGAKVVLLEGHLMGGDCLNFGCVPSKALLAAGHK
CCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECEEECCCCCCCCCCCCHHHHHCCCC
AHETSEAAFGVAGHEPSPDYAAAKDHVQAVIDEIAPVDSQERFEGLGVHVIREFGRFISE
CCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
SEVQAGAHTIKARRFVIATGSRPFVPPIPGLDTVEYHTNETIFDLRERPDHLIIIGGGPI
HHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCEEEHHHCCCCEEEEECCCCC
GMEMAQAHRRLGSRVTVLEGAKAMGKDDPEAAAIVLDNLRAEGIEIVEGALASQIKGSDG
CHHHHHHHHHHCCEEEEEECCHHCCCCCCCHHHHEEHHHCCCCHHHHHHHHHHHCCCCCC
SVTVETKDGASYEGSHLLMAVGRAVNVDKLDLEKAGVEYDRSGVKVGDDLRSTNKRVYAV
CEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCEECCHHHCCCCEEEEE
GDVAGGAQFTHVAGYHAGVIIRPMLFGLPAKARKDHIPWATYTSPELAQVGLTEAEAKEQ
ECCCCCCCEEEECCCCCCHHHHHHHHCCCCCHHCCCCCEEECCCCHHHHCCCCHHHHHHH
HGDNVFIAKAEFEHNDRGIATGQTKGFVKVMVVKGKPVGATIVGPQAGELIGIWSLAIAN
CCCEEEEEEEECCCCCCCEECCCCCCEEEEEEEECCCCCEEEECCCCCCEEEHHHHHHHH
KLKMSAVANMIAPYPTLGEINKRAASAYFTPKLFDSALVKKVVRFVQRFGRP
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STIETDICVIGAGSGGLSVAAGAVQMGAKVVLLEGHLMGGDCLNFGCVPSKALLAAGHK
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECEEECCCCCCCCCCCCHHHHHCCCC
AHETSEAAFGVAGHEPSPDYAAAKDHVQAVIDEIAPVDSQERFEGLGVHVIREFGRFISE
CCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
SEVQAGAHTIKARRFVIATGSRPFVPPIPGLDTVEYHTNETIFDLRERPDHLIIIGGGPI
HHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCEEECCCCEEEHHHCCCCEEEEECCCCC
GMEMAQAHRRLGSRVTVLEGAKAMGKDDPEAAAIVLDNLRAEGIEIVEGALASQIKGSDG
CHHHHHHHHHHCCEEEEEECCHHCCCCCCCHHHHEEHHHCCCCHHHHHHHHHHHCCCCCC
SVTVETKDGASYEGSHLLMAVGRAVNVDKLDLEKAGVEYDRSGVKVGDDLRSTNKRVYAV
CEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCEECCHHHCCCCEEEEE
GDVAGGAQFTHVAGYHAGVIIRPMLFGLPAKARKDHIPWATYTSPELAQVGLTEAEAKEQ
ECCCCCCCEEEECCCCCCHHHHHHHHCCCCCHHCCCCCEEECCCCHHHHCCCCHHHHHHH
HGDNVFIAKAEFEHNDRGIATGQTKGFVKVMVVKGKPVGATIVGPQAGELIGIWSLAIAN
CCCEEEEEEEECCCCCCCEECCCCCCEEEEEEEECCCCCEEEECCCCCCEEEHHHHHHHH
KLKMSAVANMIAPYPTLGEINKRAASAYFTPKLFDSALVKKVVRFVQRFGRP
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]