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The map label for this gene is pckA [H]

Identifier: 89053026

GI number: 89053026

Start: 524727

End: 526334

Strand: Reverse

Name: pckA [H]

Synonym: Jann_0535

Alternate gene names: 89053026

Gene position: 526334-524727 (Counterclockwise)

Preceding gene: 89053034

Following gene: 89053025

Centisome position: 12.19

GC content: 61.01

Gene sequence:

>1608_bases
ATGACCCATGCCCTTTCAGGGGGACGCGTGAACCCCGCACATACGCTTGACCAGCAAGGTATCACAGGGCTCGGCTCGGT
CTATTACAACCTGCTGGAACCCGCCCTGATGCAGGCCGCCGTCGAACGCGGCGAAGGGCGCATCGGCAAGGGGGGCACGT
TCCTGGTCTCCACCGGTCAGTTCACCGGCCGCTCCCCCAACGACAAGTTCGTCGTCCGCACGCCCGAGGTTGAAGATCAT
ATCTGGTGGGACAACAACGCGCCCATGACCCCCGACGCATTTGATGCGCTCTACAGCGATATGCTGGAGCACATGAAGGG
CGGCGACTATTACGTGCAGGATCTGTTCGGCGGCGCAGATGCGGAGCATCGGCTTGACGTGCGCGTCGTGTCTGAACTCG
CCTGGCACAACCTCTTCATCCGGCATCTGCTGCGCCGTCCCGATGCGGAGGAGCTTGCGTCCTTCGTGCCGGAATTCACC
ATCATCAACTGCCCGAGCTTCAAGGCGGACCCAGCGCGTCACGGTACAAATTCCGAGACTGTGATCGCGCTGAATTTCGA
GCGGAAACTGATCCTGATCGGCAATACCGAATATGCGGGCGAGAACAAGAAATCCGTGTTCACGCTGCTGAACTACATTC
TGCCCGGCAAAGGTGTGATGGCCATGCATTGCTCGGCCAACCACGCCATCGGCAATCCGGATGACAGCGCGGTGTTCTTC
GGCCTCTCGGGCACCGGCAAGACGACCCTGTCCGCCGATCCCGGTCGGACGCTGATCGGCGATGATGAACATGGTTGGTC
CGATAGCGGCATCTTCAATTTTGAAGGGGGCTGCTATGCCAAGACCATCAACCTGCGCGAAGACGCGGAGCCTGAAATCT
TTGCAACGACGGCCAAGTTTTCCACCGTGATCGAGAACATGGTCTATGACGAAGATACGCTGGAGCTGGACTTTGACGAC
GACAGCCTGACGGCCAATATGCGCTGTGCCTACCCGATCGAGATGATCTCCAACGCGTCCGCCACCGGGCTTGGCGGCTC
GCCCAAGAACATCGTGATGCTGACCTGCGATGCCTTTGGCGTTCTGCCCCCGATTGCTCGGCTGTCGCCCGCCCAGGCGA
TGTACCACTTCCTGTCTGGCTTTACCGCAAAGGTCGCCGGGACAGAGCGTGGCGTGACCGAGCCGACGCCCACCTTCTCC
ACCTGTTTCGGCGCGCCCTTCATGCCGCGTCGACCCGAGGTCTATGGCGATCTGTTGCGCCAGAAGATTGCCGCCCATGG
CGCCACCTGTTGGCTGGTGAACACGGGCTGGACCGGCGGCGCGTTCGGGACCGGCTCCCGCATGCCGATCCGGGCCACAA
GGGCGTTGCTGACGGCAGCCCTCAACGGGACGTTGAACGCCGGAGATTTCCGCCGCGATCCAAACTTCGGCTTTGATGTA
CCAGTCCATTGTCACGGCGTGGCAGATCTGCTGTTGGACCCCCGCCGCACCTGGGACCGCCCCGACAGCTATGACCGTCA
GGCTCAACGCCTGGTCGACATGTTCTCCGATAACTTTGCCCAATATGTCGACCACATCGACGACGATGTGAAAGCGGCTG
CCATCTAA

Upstream 100 bases:

>100_bases
TCGCAAAAACGTCATGTTATAGCGGTCTCACGACGCAGCAATTGCGCTGTCTCGTCACCGAAACCAGGGCGTCAGGCCGC
CCGACAGGAGCTTCAGAGCC

Downstream 100 bases:

>100_bases
ACCCTGTGCATATCGCCCACGACTTACGCGGCCATCCCACGGGGTGGCCGTTCGCGTAACTGCGGGAAATCCAGATGTAG
GCAGAGGCAGTCTCGCGCGT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTHALSGGRVNPAHTLDQQGITGLGSVYYNLLEPALMQAAVERGEGRIGKGGTFLVSTGQFTGRSPNDKFVVRTPEVEDH
IWWDNNAPMTPDAFDALYSDMLEHMKGGDYYVQDLFGGADAEHRLDVRVVSELAWHNLFIRHLLRRPDAEELASFVPEFT
IINCPSFKADPARHGTNSETVIALNFERKLILIGNTEYAGENKKSVFTLLNYILPGKGVMAMHCSANHAIGNPDDSAVFF
GLSGTGKTTLSADPGRTLIGDDEHGWSDSGIFNFEGGCYAKTINLREDAEPEIFATTAKFSTVIENMVYDEDTLELDFDD
DSLTANMRCAYPIEMISNASATGLGGSPKNIVMLTCDAFGVLPPIARLSPAQAMYHFLSGFTAKVAGTERGVTEPTPTFS
TCFGAPFMPRRPEVYGDLLRQKIAAHGATCWLVNTGWTGGAFGTGSRMPIRATRALLTAALNGTLNAGDFRRDPNFGFDV
PVHCHGVADLLLDPRRTWDRPDSYDRQAQRLVDMFSDNFAQYVDHIDDDVKAAAI

Sequences:

>Translated_535_residues
MTHALSGGRVNPAHTLDQQGITGLGSVYYNLLEPALMQAAVERGEGRIGKGGTFLVSTGQFTGRSPNDKFVVRTPEVEDH
IWWDNNAPMTPDAFDALYSDMLEHMKGGDYYVQDLFGGADAEHRLDVRVVSELAWHNLFIRHLLRRPDAEELASFVPEFT
IINCPSFKADPARHGTNSETVIALNFERKLILIGNTEYAGENKKSVFTLLNYILPGKGVMAMHCSANHAIGNPDDSAVFF
GLSGTGKTTLSADPGRTLIGDDEHGWSDSGIFNFEGGCYAKTINLREDAEPEIFATTAKFSTVIENMVYDEDTLELDFDD
DSLTANMRCAYPIEMISNASATGLGGSPKNIVMLTCDAFGVLPPIARLSPAQAMYHFLSGFTAKVAGTERGVTEPTPTFS
TCFGAPFMPRRPEVYGDLLRQKIAAHGATCWLVNTGWTGGAFGTGSRMPIRATRALLTAALNGTLNAGDFRRDPNFGFDV
PVHCHGVADLLLDPRRTWDRPDSYDRQAQRLVDMFSDNFAQYVDHIDDDVKAAAI
>Mature_534_residues
THALSGGRVNPAHTLDQQGITGLGSVYYNLLEPALMQAAVERGEGRIGKGGTFLVSTGQFTGRSPNDKFVVRTPEVEDHI
WWDNNAPMTPDAFDALYSDMLEHMKGGDYYVQDLFGGADAEHRLDVRVVSELAWHNLFIRHLLRRPDAEELASFVPEFTI
INCPSFKADPARHGTNSETVIALNFERKLILIGNTEYAGENKKSVFTLLNYILPGKGVMAMHCSANHAIGNPDDSAVFFG
LSGTGKTTLSADPGRTLIGDDEHGWSDSGIFNFEGGCYAKTINLREDAEPEIFATTAKFSTVIENMVYDEDTLELDFDDD
SLTANMRCAYPIEMISNASATGLGGSPKNIVMLTCDAFGVLPPIARLSPAQAMYHFLSGFTAKVAGTERGVTEPTPTFST
CFGAPFMPRRPEVYGDLLRQKIAAHGATCWLVNTGWTGGAFGTGSRMPIRATRALLTAALNGTLNAGDFRRDPNFGFDVP
VHCHGVADLLLDPRRTWDRPDSYDRQAQRLVDMFSDNFAQYVDHIDDDVKAAAI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=537, Percent_Identity=44.87895716946, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322950, Length=506, Percent_Identity=45.4545454545455, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58512; Mature: 58381

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHALSGGRVNPAHTLDQQGITGLGSVYYNLLEPALMQAAVERGEGRIGKGGTFLVSTGQ
CCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
FTGRSPNDKFVVRTPEVEDHIWWDNNAPMTPDAFDALYSDMLEHMKGGDYYVQDLFGGAD
CCCCCCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHCCCC
AEHRLDVRVVSELAWHNLFIRHLLRRPDAEELASFVPEFTIINCPSFKADPARHGTNSET
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCE
VIALNFERKLILIGNTEYAGENKKSVFTLLNYILPGKGVMAMHCSANHAIGNPDDSAVFF
EEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEE
GLSGTGKTTLSADPGRTLIGDDEHGWSDSGIFNFEGGCYAKTINLREDAEPEIFATTAKF
EECCCCCEEECCCCCCCEECCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCEEEEHHHH
STVIENMVYDEDTLELDFDDDSLTANMRCAYPIEMISNASATGLGGSPKNIVMLTCDAFG
HHHHHHHHCCCCCEEEECCCCCCEECEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCC
VLPPIARLSPAQAMYHFLSGFTAKVAGTERGVTEPTPTFSTCFGAPFMPRRPEVYGDLLR
CCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHH
QKIAAHGATCWLVNTGWTGGAFGTGSRMPIRATRALLTAALNGTLNAGDFRRDPNFGFDV
HHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PVHCHGVADLLLDPRRTWDRPDSYDRQAQRLVDMFSDNFAQYVDHIDDDVKAAAI
CEEECCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
THALSGGRVNPAHTLDQQGITGLGSVYYNLLEPALMQAAVERGEGRIGKGGTFLVSTGQ
CCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
FTGRSPNDKFVVRTPEVEDHIWWDNNAPMTPDAFDALYSDMLEHMKGGDYYVQDLFGGAD
CCCCCCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHCCCC
AEHRLDVRVVSELAWHNLFIRHLLRRPDAEELASFVPEFTIINCPSFKADPARHGTNSET
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCE
VIALNFERKLILIGNTEYAGENKKSVFTLLNYILPGKGVMAMHCSANHAIGNPDDSAVFF
EEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEE
GLSGTGKTTLSADPGRTLIGDDEHGWSDSGIFNFEGGCYAKTINLREDAEPEIFATTAKF
EECCCCCEEECCCCCCCEECCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCEEEEHHHH
STVIENMVYDEDTLELDFDDDSLTANMRCAYPIEMISNASATGLGGSPKNIVMLTCDAFG
HHHHHHHHCCCCCEEEECCCCCCEECEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCC
VLPPIARLSPAQAMYHFLSGFTAKVAGTERGVTEPTPTFSTCFGAPFMPRRPEVYGDLLR
CCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHH
QKIAAHGATCWLVNTGWTGGAFGTGSRMPIRATRALLTAALNGTLNAGDFRRDPNFGFDV
HHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PVHCHGVADLLLDPRRTWDRPDSYDRQAQRLVDMFSDNFAQYVDHIDDDVKAAAI
CEEECCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA