Click here to switch to the map view.

The map label for this gene is dfrA [H]

Identifier: 89053020

GI number: 89053020

Start: 518690

End: 519688

Strand: Reverse

Name: dfrA [H]

Synonym: Jann_0529

Alternate gene names: 89053020

Gene position: 519688-518690 (Counterclockwise)

Preceding gene: 89053021

Following gene: 89053019

Centisome position: 12.04

GC content: 62.96

Gene sequence:

>999_bases
ATGCCAAACCACGTCTTTCTGACCGGTGTCACCGGCTTCATCGCCAAACGCATCGCGCTGGACCTCCTGAATGGGGGGCA
TACAGTCACCGGCTCTTTGCGAAACCCGGCACGCGCCGATGAGGTGCGCGATGCCCTGCATCCACATCTGACAGACCCCG
GGTCGCTCGAACACCTGACATTCGCGACGCTCGACCTGACCCGTGATGAGGGCTGGACGGACGCCATGGCGGGCTGCGAC
ACGCTGATGCACACCGCCTCCCCCTTCCCCCTGTCCTCTCCCAAGGATGAGAATGACCTGATCCGTCCCGCTGTGGATGG
CACGCTGCGGGCGCTGAATGCGGCGAAAGACGCAGGTGTGACCCGGGTGATCCTGACGTCGTCTGTCGTGGCGATTGAGG
CCAATGACAGGCCCAACCCGCAGACGCCTGCCAATTGGACCGACGTCAACCACCCCCGCGCGACGCCCTACTATACATCC
AAGACGTTGGCAGAACGCGCGGCCTGGGATTTCGTGGCCGAACATCCGGAGATGCAGCTGACAACCATCAACCCGTCCCT
CGTTCTCGGCGCCCCGCTGGACGACAATTACGGTACGTCGCTGTCGCTGATTGAGCGGATGATGGCCGGAAAGGATCCGG
CTTTGCCGCCACTTGGGTTTGGGCTGGTGGATGTTGAGGATGTTTCAGCCATGCATATCGCCGCCATGGCGCGGCCTGAA
AGCGCCGGAAACCGCTACATCGCCTCGGCCGGGTCGATGATGATGCCCGCCATCGGCGCACATCTGAAAGGCATTTATCC
GGATCGCAAGATCGCCACGCGGCAGGCACCGGGATGGCTGCTGAGGGTGCTGGCACTCTTTGACCCGTCGATCCGTACGA
TTTTGCCACAACTGGGCCATATGCCGGAGTTCGACACCTCGGTCAGCATCCGTGATCTGGGGATCACGTTCACCCCCGCC
ACCACGGCCCTGAAGCGCGCCGCGGACGCCGTCACCTGA

Upstream 100 bases:

>100_bases
GCATAGGCCCGTTACCAATCGATTTCTGACATTCTGACAATTTGACTTGAATTTGTCAGAAGAAGCTTACCTATGGGGTG
TCTGATAGCGGAGCCTCCCC

Downstream 100 bases:

>100_bases
ATGCGGGCCTTTGTTGCTCTGCCTCTCACCGATCCGGCCATCGACGCTTTGACGCGAGTGCAATCCACGCTTCCCACGGG
CAAGCCAGTGCCGGAGGAGA

Product: dihydrokaempferol 4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPNHVFLTGVTGFIAKRIALDLLNGGHTVTGSLRNPARADEVRDALHPHLTDPGSLEHLTFATLDLTRDEGWTDAMAGCD
TLMHTASPFPLSSPKDENDLIRPAVDGTLRALNAAKDAGVTRVILTSSVVAIEANDRPNPQTPANWTDVNHPRATPYYTS
KTLAERAAWDFVAEHPEMQLTTINPSLVLGAPLDDNYGTSLSLIERMMAGKDPALPPLGFGLVDVEDVSAMHIAAMARPE
SAGNRYIASAGSMMMPAIGAHLKGIYPDRKIATRQAPGWLLRVLALFDPSIRTILPQLGHMPEFDTSVSIRDLGITFTPA
TTALKRAADAVT

Sequences:

>Translated_332_residues
MPNHVFLTGVTGFIAKRIALDLLNGGHTVTGSLRNPARADEVRDALHPHLTDPGSLEHLTFATLDLTRDEGWTDAMAGCD
TLMHTASPFPLSSPKDENDLIRPAVDGTLRALNAAKDAGVTRVILTSSVVAIEANDRPNPQTPANWTDVNHPRATPYYTS
KTLAERAAWDFVAEHPEMQLTTINPSLVLGAPLDDNYGTSLSLIERMMAGKDPALPPLGFGLVDVEDVSAMHIAAMARPE
SAGNRYIASAGSMMMPAIGAHLKGIYPDRKIATRQAPGWLLRVLALFDPSIRTILPQLGHMPEFDTSVSIRDLGITFTPA
TTALKRAADAVT
>Mature_331_residues
PNHVFLTGVTGFIAKRIALDLLNGGHTVTGSLRNPARADEVRDALHPHLTDPGSLEHLTFATLDLTRDEGWTDAMAGCDT
LMHTASPFPLSSPKDENDLIRPAVDGTLRALNAAKDAGVTRVILTSSVVAIEANDRPNPQTPANWTDVNHPRATPYYTSK
TLAERAAWDFVAEHPEMQLTTINPSLVLGAPLDDNYGTSLSLIERMMAGKDPALPPLGFGLVDVEDVSAMHIAAMARPES
AGNRYIASAGSMMMPAIGAHLKGIYPDRKIATRQAPGWLLRVLALFDPSIRTILPQLGHMPEFDTSVSIRDLGITFTPAT
TALKRAADAVT

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=332, Percent_Identity=35.8433734939759, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324421, Length=287, Percent_Identity=31.0104529616725, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321281, Length=314, Percent_Identity=31.8471337579618, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320750, Length=276, Percent_Identity=31.1594202898551, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321399, Length=264, Percent_Identity=31.0606060606061, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 35679; Mature: 35548

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHVFLTGVTGFIAKRIALDLLNGGHTVTGSLRNPARADEVRDALHPHLTDPGSLEHLT
CCCCEEEECHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEE
FATLDLTRDEGWTDAMAGCDTLMHTASPFPLSSPKDENDLIRPAVDGTLRALNAAKDAGV
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
TRVILTSSVVAIEANDRPNPQTPANWTDVNHPRATPYYTSKTLAERAAWDFVAEHPEMQL
EEEEEECEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
TTINPSLVLGAPLDDNYGTSLSLIERMMAGKDPALPPLGFGLVDVEDVSAMHIAAMARPE
EEECCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCC
SAGNRYIASAGSMMMPAIGAHLKGIYPDRKIATRQAPGWLLRVLALFDPSIRTILPQLGH
CCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCC
MPEFDTSVSIRDLGITFTPATTALKRAADAVT
CCCCCCCEEEEECCEEECCHHHHHHHHHHCCC
>Mature Secondary Structure 
PNHVFLTGVTGFIAKRIALDLLNGGHTVTGSLRNPARADEVRDALHPHLTDPGSLEHLT
CCCEEEECHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEE
FATLDLTRDEGWTDAMAGCDTLMHTASPFPLSSPKDENDLIRPAVDGTLRALNAAKDAGV
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
TRVILTSSVVAIEANDRPNPQTPANWTDVNHPRATPYYTSKTLAERAAWDFVAEHPEMQL
EEEEEECEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
TTINPSLVLGAPLDDNYGTSLSLIERMMAGKDPALPPLGFGLVDVEDVSAMHIAAMARPE
EEECCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCC
SAGNRYIASAGSMMMPAIGAHLKGIYPDRKIATRQAPGWLLRVLALFDPSIRTILPQLGH
CCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCC
MPEFDTSVSIRDLGITFTPATTALKRAADAVT
CCCCCCCEEEEECCEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]